Deproteinizing Sample Preparation Kit - TCA ab204708 from Abcam

Product Specs
ItemDeproteinizing Sample Preparation Kit - TCA
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab204708
Quantity200 Test
Sample TypeUrine, Whole Blood, Tissue Extracts, Tissue Culture Media, Tissue Lysate
Assay Time0h 25m

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(41) Frequency-Dependent Antioxidant Responses in HT-1080 Human Fibrosarcoma Cells Exposed to Weak Radio Frequency FieldsAntioxidants (Basel)October 15, 2024Hakki Gurhan, Frank Barnesice. Deproteinization of cell lysates was performed using the Deproteinizing Sample Preparation Kit—TCA (Cat. No. ab204708, Abcam, Cambridge, UK). For samples with protein concentrations less than 25 mg/mL, 150 µL of the sample

(42) Stress triggers gut dysbiosis via CRH-CRHR1-mitochondria pathwaynpj Biofilms and MicrobiomesSeptember 30, 2024Yiming Zhang, Xiaoang Li, Siqi Lu, Huaizhu Guo, Zhuangyi Zhang, Haonan Zheng, Cunzheng Zhang, Jindong Zhang, Kun Wang, Fei Pei, et al.epithelium was isolated and then deproteinized using the Deproteinizing Sample Preparation Kit (Abcam, ab204708) following the manufacturer’s instructions. Lactate measurement in the colon epithelium lysates was

(43) Astrocytic pyruvate dehydrogenase kinase-lactic acid axis involvement in glia-neuron crosstalk contributes to morphine-induced hyperalgesia in miceFundamental ResearchJuly 1, 2024Xiaqing Ma, Qi Qi, Wenying Wang, Min Huang, Haiyan Wang, Limin Luo, Xiaotao Xu, Tifei Yuan, Haibo Shi, Wei Jiang, et al.L4 and L5 segments tissues/cell supernatants deproteinized by deproteinizing sample preparation kit (ab204708; Abcam). The soluble fraction was assayed using a Lactate Assay Kit (ab65331; Abcam). The samples were

(44) A positive feedback inhibition of isocitrate dehydrogenase 3β on paired-box gene 6 promotes Alzheimer-like pathologySignal Transduction and Targeted TherapyApril 29, 2024Xin Wang, Qian Liu, Hai-tao Yu, Jia-zhao Xie, Jun-ning Zhao, Zhi-ting Fang, Min Qu, Yao Zhang, Ying Yang, Jian-Zhi Wangmeasured utilizing the ATP Assay Kit (S0027, Beyotime). The Deproteinizing Sample Preparation Kit—TCA (ab204708, Abcam) was employed to remove proteins from the samples. Details of the reagents and resources utilized

(45) Autofluorescence is a biomarker of neural stem cell activation stateCell Stem CellApril 4, 2024Christopher S. Morrow, Kelsey Tweed, Sabina Farhadova, Alex J. Walsh, Bo P. Lear, Avtar Roopra, Ryan D. Risgaard, Payton C. Klosa, Zachary P. Arndt, Ella R. Peterson, et al.(Thermo Fisher Scientific) at 750 nm. Samples were deproteinized with Abcam’s deproteinization kit (ab204708). Then the samples were assayed (normalized by protein load) by preparing FAD reaction mix per the manufacturer’s

(46) Endocrine-disrupting compounds and their impact on human placental function: Evidence from placenta organ- on-chip studiesLab on a ChipMarch 9, 2024Manuel S. Vidal, Lauren S. Richardson, Ananth Kumar Kumar Kammala, Sungjin Kim, Po Yi Lam, Rahul Cherukuri, Tilu Jain Thomas, Mohammed Bettayeb, Arum Han, Ivan Rusyn, et al.× 20 min × 4 °C. Then the supernatants were deproteinized using a commercial deproteinization kit (ab204708, Abcam). Afterward, 50 uL of the supernatant was added into specified wells in a 96-well plate. Standards

(47) Vitamin‐C‐dependent downregulation of the citrate metabolism pathway potentiates pancreatic ductal adenocarcinoma growth arrestMolecular OncologyFebruary 29, 2024Aiora Cenigaonandia‐Campillo, Ana Garcia‐Bautista, Anxo Rio‐Vilariño, Arancha Cebrian, Laura del Puerto, José Antonio Pellicer, José Antonio Gabaldón, Horacio Pérez‐Sánchez, Miguel Carmena‐Bargueño, Carolina Meroño, et al.tissue was harvested and homogenized using a dounce homogenized. Samples were deproteinized using Abcam ab204708 kit according to manufacturer's instructions. 2.7 Inhibition growth (IG) assays IG assays were performed

(48) PARP-1 is a transcriptional rheostat of metabolic and bivalent genes during developmentLife Science AllianceFebruary 1, 2024Gbolahan Bamgbose, Alexei Tulincolorimetric signals at 570 nm, samples were deproteinized with a Deproteinizing Sample Preparation Kit (ab204708; Abcam). Signal quantification was carried out using a Biotek Cytation 3 Imager. Statistical analyses Results

(49) A role for the cystathionine-β-synthase /H2S axis in astrocyte dysfunction in the aging brainRedox BiologyDecember 1, 2023Anindya Dey, Pijush Kanti Pramanik, Shailendra Kumar Dhar Dwivedi, Fiifi Neizer-Ashun, Tamas Kiss, Abhrajit Ganguly, Heather Rice, Priyabrata Mukherjee, Chao Xu, Mohiuddin Ahmad, et al.at 13,000g for 15 min. Supernatant was deproteinized following manufacturer's instructions (Abcam, ab204708). Resultant supernatant was used to determine reduced GSH, total GSH and oxidized GSSG, which was calculated

(50) Neuronal Glycogen Breakdown Mitigates Tauopathy via Pentose Phosphate Pathway-Mediated Oxidative Stress ReductionResearch SquareDecember 1, 2023Sudipta Kapahi, Kenneth A. Wilson, Tyler A.U. Hilsabeck, Sydney Hilsabeck, Eric B. Alderfer, Jordan B Dammer, Jordan Burton, Samah Holtz, Enrique M. Carrera, Jennifer N. Carrera, et al.Deproteinized tissue lysate was used for the assay. Deproteinization was performed using a kit from Abcam (#ab204708). PKA activity was measured using a kit protocol (ThermoFischer# EIAPKA). For PKA activity, 80 fly heads

(51) Fenofibrate Decreases Ethanol-Induced Neuroinflammation and Oxidative Stress and Reduces Alcohol Relapse in Rats by a PPAR-α-Dependent MechanismAntioxidants (Basel)September 13, 2023Cristina Ibáñez, Tirso Acuña, María Elena Quintanilla, Diliana Pérez-Reytor, Paola Morales, Eduardo Karahaniandebris removal, and deproteinized to remove enzymes that could potentially metabolize glutathione (Deproteinizing Sample Preparation Kit—TCA, Abcam, Boston, MA, USA). 2.4. Determination of Microglia Immunoreactivity The other cerebral hemisphere

(52) Succinate mediates inflammation-induced adrenocortical dysfunctionElifeJuly 14, 2023Ivona Mateska, Anke Witt, Eman Hagag, Anupam Sinha, Canelif Yilmaz, Evangelia Thanou, Na Sun, Ourania Kolliniati, Maria Patschin, Heba Abdelmegeed, et al.100 μl ATP assay buffer. Samples were cleared using the Deproteinizing Sample Preparation Kit – TCA (ab204708, Abcam). Samples were incubated for 30 min with the ATP reaction mix and fluorescence (Ex/Em = 535/587

(53) A familial natural short sleep mutation promotes healthy aging and extends lifespan in DrosophilaResearch SquareJune 30, 2023Pritika Pandey, P. Kerr Wall, Stephen R. Lopez, Olga S. Dubuisson, Elizabeth R.M. Zunica, Wagner S. Dantas, John P. Kirwan, Christopher L. Axelrod, Alyssa E. Johnsonenzymatic assay (Abcam, ab204710). Isolated thorax muscles (~5/biological replicate) were deproteinated (Abcam, ab204708), homogenized in ice cold FAS assay buffer, and centrifuged at 4°C at 10,000 × g for 5 minutes to remove

(54) Limiting Mrs2-dependent mitochondrial Mg(2+) uptake induces metabolic programming in prolonged dietary stressCell reportsMarch 28, 2023Madaris TR, Venkatesan M, Maity S, Stein MC, Vishnu N, Venkateswaran MK, Davis JG, Ramachandran K, Uthayabalan S, Allen C, et al.When required, deproteination was accomplished using a trichloroacetic acid precipitation kit (Abcam ab204708) for tissue and cells, or 10,000 MWCO spin column for plasma. Gene expression studies (RT-qPCR, RNA-seq,

(55) Neutrophil-activating therapy for the treatment of cancerCancer cellFebruary 13, 2023Linde IL, Prestwood TR, Qiu J, Pilarowski G, Linde MH, Zhang X, Shen L, Reticker-Flynn NE, Chiu DK, Sheu LY, et al.and clarified lysate was deproteinized using the Deproteinizing Sample Preparation Kit – TCA (Abcam ab204708) according to the manufacturer’s instructions. Glutathione was detected using the GSH/GSSG Ratio Detection

(56) High glucose promotes regulatory T cell differentiationPloS oneJanuary 1, 2023Pitmon E, Meehan EV, Ahmadi E, Adler AJ, Wang K.deproteinized to remove LDH and other proteins using a Deproteinizing Sample Preparation Kit-TCA (Abcam, Cat # ab204708) prior to lactate measurement. To observe absorption of lactate by fully differentiated Treg cells,

(57) A Drosophila model of the neurological symptoms in Mpv17-related diseasesScientific ReportsDecember 31, 2022Atsushi Kodani, Mizuki Yamaguchi, Ririka Itoh, Man Anh Huynh, Hideki Yoshidafor 10 min. The supernatant was then transferred to a new tube, 10 µL of cold trichloroacetic acid (ab204708; Abcam) was added to the supernatant, and the sample was kept on ice for 15 min. After centrifugation

(58) Blocking autophagy with chloroquine aggravates lipid accumulation and reduces intracellular energy synthesis in hepatocellular carcinoma cells, both contributing to its anti-proliferative effectJournal of Cancer Research and Clinical OncologyDecember 1, 2022Fengming Xu, Hans-Michael Tautenhahn, Olaf Dirsch, Uta Dahmencells were harvested and lysed to prepare cells lysates. Deproteinizing Sample Preparation Kit (Abcam, #ab204708) was used to remove superfluous enzymes. 50 μl reaction mixture and background mixture were prepared

(59) FGF21 controls hepatic lipid metabolism via sex-dependent interorgan crosstalkJCI InsightOctober 10, 2022Aki T. Chaffin, Karlton R. Larson, Kuei-Pin Huang, Chih-Ting Wu, Nadejda Godoroja, Yanbin Fang, Devi Jayakrishnan, Karla A. Soto Sauza, Landon C. Sims, Niloufar Mohajerani, et al.including an optional deproteinization step using an additional commercially available kit (Abcam, catalog ab204708). Gene expression analysis. Tissues were collected at euthanasia and frozen in liquid nitrogen (liver,

(60) Hypoxic condition induced H3K27me3 modification of the LncRNA Tmem235 promoter thus supporting apoptosis of BMSCsApoptosis : an international journal on programmed cell deathOctober 1, 2022Zhang F, Luo H, Peng W, Wang L, Wang T, Xie Z, Zhang J, Dong W, Zheng X, Liu G, et al.and centrifuged at 4 °C and 15,000 g for 8 min. The collected supernatant was deproteinized with the Deproteinizing Sample Preparation Kit—TCA (Abcam) to remove interfering enzymes. Next, 5 μL of enzyme-depleted supernatant was added to a 96-well

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Deproteinizing Sample Preparation Kit - TCA from Abcam

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Deproteinizing Sample Preparation Kit - TCA from Abcam

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