MojoSort™ Mouse CD4 T Cell Isolation Kit 480033 from BioLegend

Product Specs
ItemMojoSort™ Mouse CD4 T Cell Isolation Kit
CompanyBioLegend
Price $654.00Supplier Page View Company Product Page
Catalog Number480033
Quantity200 tests
ApplicationsCell Separation (MojoSort™, ) - Quality tested
FormatCocktail: Phosphate-buffered solution containing 0.09% sodium azide, pH 7.2.
Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide
ReactivityMouse
Detection TargetCD4
Cell Type (standardized)CD4+ T Cell

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) IL-33 promotes transcriptional and metabolic adaptations of tissue-resident Th2 cellsJ ImmunolApril 16, 2026Anna K Kania, David E Sanin, Xinyue Gu, Mia Gidley, Eryk Kokosinski, Allen Smith, Erika Pearce, Edward J Pearcelymph nodes (mLN) of naive or Hpoly-infected mice using MojoSort™ Mouse CD4 T Cell Isolation Kit (Biolegend, 480033). The cells were counted and plated at 0.5 million cells/ml (1.2 ml in 12 well plate) in T cell media

(2) Simvastatin Alleviates ConA-Induced Autoimmune Hepatitis by Inhibiting CD4+ T Cell Activation via Calcium-NFATC3 PathwayInflammationJanuary 30, 2026Xiyu Wang, Tianhang Long, Longyang Zhou, Nan Xu, Peiyang Fang, Buer Li, Haozhe Xu, Guangyong Sun, Dong Zhang, Hua Jinerythrocyte lysis, and CD4+ T cells were isolated using the MojoSort™ Mouse CD4+ T Cell Isolation Kit (480033, Biolegend, USA). Sorted CD4+ T cells or splenocytes (5 × 10⁵ cells/well) were seeded in 96-well U-bottom

(3) CCL5 deficiency aggravates acute DSS-induced colitis by restricting IL-33-induced formation of Tregs in intestinal tractClinical ScienceJanuary 14, 2026Yang Luo, Ting-Yue Gong, Yong-Heng Zhao, Hao Li, Guang-Yao Ye, Zi-Zhen Zhang, Min-Hao Yu, Yan Zhang, Ming Zhongwere negatively selected from intestinal lymph nodes utilizing the Mouse CD4+ T Cell Isolation Kit (480033, BioLegend, San Diego, CA, USA). The lymphocytes, isolated as described above, were first adjusted to

(4) Distinct components of mRNA vaccines cooperate to instruct efficient germinal center responsesCellDecember 24, 2025Diana Castaño, Emily Bettini, Binod Kumar, Aleksey Chudnovskiy, Anna Siv, Giulia Protti, Sandra Nakadakari-Higa, Simona Ceglia, Nina De Luna, Joy E. Chiu, et al.al.83,https://www.bioconductor.org/packages/release/bioc/html/UCell.html Other,, MojosortTM Mouse CD4 T cell isolation kit,Biolegend,Cat#480033 Nunc Maxisorp flat-bottom 96-well plates,Thermo Scientific,Cat#442404 Qiagen RNeasy Micro kit,Qiagen,Cat#74004 SYBR

(5) CGRP restrains CD4+ T cell responses and allergic sensitizationFront ImmunolNovember 18, 2025Xiaoshi Li, Ying Zhang, Wenlong Chen, Qingren Meng, Ai Huang, Jiayi Pan, Duan Chen, Yue Xiao, Jialin Wei, Heng Sun, et al.were isolated from spleens or medLNs via the MojoSort Mouse CD4 T Cell Isolation Kit (Biolegend, USA, 480033). Total RNA was extracted via the FastPure Cell/Tissue Total RNA Isolation Kit V2 (Vazyme, China, RC112-01),

(6) Increased neutrophil senescence is associated with impaired immunosuppressive activity in systemic lupus erythematosusActa Biochimica et Biophysica SinicaNovember 1, 2025Lei Han, Fengling Huang, Qingchen Zhu, Huan Wang, Tianlin Lu, Chunyuan Xiao, Jing Xu, Xiaoyan Zhang, Yichuan Xiao, Xinfang HuangNK Cell Isolation kit (130-115-818; Miltenyi Biotec) or the MojoSort™ Mouse CD4 T-Cell Isolation kit (480033; Biolegend). The detailed steps were performed according to the manufacturer’s instructions. Cell co-culture NK cells

(7) A next-generation anti-CTLA-4 probody mitigates toxicity and enhances anti-tumor immunity in miceNat CommunOctober 10, 2025Weian Cao, Junfan Chen, Yutong Fu, Haitao Jiang, Yu Gao, Huiming Huang, Yang-Xin Fu, Wenyan Wangseparated, and total CD4 T cells were purified by MojoSort Mouse CD4 T Cell Isolation Kit (Biolegend, 480033). Foxp3-YFP Treg cells were then sorted by FACS Aria SORP based on the fluorescence of GFP. 0.1 million

(8) Genomic Analysis Defines Increased Circulating, Leukemia-Induced Macrophages That Promote Immune Suppression in Mouse Models of FGFR1-Driven LeukemogenesisCellsSeptember 30, 2025Ting Zhang, Atsuko Matsunaga, Xiaocui Lu, Hui Fang, Nandini Chatterjee, Ahmad Alimadadi, Stephanie F. Mori, Xuexiu Fang, Gavin Wang, Huidong Shi, et al.Spleen-derived CD4+ T cells were isolated using the MojoSort™ Mouse CD4 T Cell Isolation Kit (Biolegend, #480033) from the naïve C57BL6 mice. The isolated CD4+ T cells were first stained using the CFSE Cell Division Tracker Kit

(9) Activated T cells break tumor immunosuppression by macrophage re-educationCancer DiscoveryJuly 3, 2025Rosa Trotta, Silvia Rivis, Shikang Zhao, Marie-Pauline Orban, Sarah Trusso Cafarello, Iris Charatsidou, Joanna Pozniak, Jonas Dehairs, Lotte Vanheer, Carlos A. Pulido Vicuna, et al.mouse CD8+ T-cell Isolation Kit (BioLegend, 480035) or the mouse CD4+ T-cell Isolation Kit (BioLegend, 480033) according to the manufacturer’s instructions. For naïve T-cell activation, cells were cultured for

(10) KMT2D coordinates antiviral CD4+ T cell responses through opposing effects on T follicular helper and cytotoxic gene expressionCell ReportsJune 24, 2025Jonathan A. Cohen, Ashlyn A. Buzzelli, Gabrielle Quickstad, Lixin Yang, Ichiro Misumi, Karl B. Shpargel, Jason K. WhitmireCompensation Bead Kit,Thermofisher,Cat# A10346 MojoSort™ mouse CD4 T cell Isolation Kit,Biolegend,Cat# 480033 MojoSort™ Streptavidin Nanobeads,Biolegend,Cat# 480016 UltraComp eBeads™,Thermofisher,Cat# 01-2222-42 UltraComp

(11) Inducible protein degradation reveals inflammation-dependent function of the Treg cell lineage-defining transcription factor Foxp3Sci ImmunolJune 6, 2025Christina Jäger, Polina Dimitrova, Qiong Sun, Jesse Tennebroek, Elisa Marchiori, Markus Jaritz, Rene Rauschmeier, Guillem Estivill, Anna Obenauf, Meinrad Busslinger, et al.cells were enriched for CD4 T cells using the Mojosort™ Mouse CD4 T Cell Isolation Kit (BioLegend cat # 480033), stained with antibody cocktails as described, and sorted on BD Aria II. T cell transfer 3x105 FACS-purified

(12) Inhibition of EED-mediated histone methylation alleviates neuroinflammation by suppressing WNT-mediated dendritic cell migrationJournal of neuroinflammationApril 1, 2025Hong W, Ma H, Li Z, Du Y, Xia W, Yin H, Huang H, Sun Z, Gai R, Tong L, et al.isolated from the spleens and lymph nodes using the MojoSort™ Mouse CD4 T Cell Isolation Kit (Biolegend, 480033), following the manufacturer’s protocols. Subsequently, BMDCs and CD4+ T cells were co-cultured at a

(13) Maresin-1 promotes neuroprotection and modulates metabolic and inflammatory responses in disease-associated cell types in preclinical models of multiple sclerosisJournal of Biological ChemistryMarch 1, 2025Insha Zahoor, Mohammad Nematullah, Mohammad Ejaz Ahmed, Mena Fatma, Mir Sajad, Kameshwar Ayasolla, Mirela Cerghet, Suresh Palaniyandi, Veronica Ceci, Giulia Carrera, et al.(#130-110-443; ∼92% purity). CD4+ cells were also isolated from spleens using a CD4 isolation kit (BioLegend # 480033; ∼94% purity). Adoptive transfer For adoptive transfer experiments, EAE was induced in SJL mice, and

(14) CD301b+ dendritic cell-derived IL-2 dictates CD4+ T helper cell differentiationNat CommunFebruary 26, 2025Naoya Tatsumi, Jihad El-Fenej, Alejandro Davila-Pagan, Yosuke Kumamotonaïve OT-II strains with Mouse CD4+ T Cell Isolation Kit (STEMCELL Technologies 19852 or BioLegend 480033) and labeled with 1.0 µM CFSE (Thermo Fisher) or CTV (C34571, Thermo Fisher) according to the manufacturer’s

(15) CD4+ T cell–innate immune crosstalk is critical during Staphylococcus aureus craniotomy infectionJCI InsightFebruary 24, 2025Gunjan Kak, Zachary Van Roy, Rachel W. Fallet, Lee E. Korshoj, Tammy Kielianfrom spleens of 6- to 8-week-old C57BL/6J mice using CD4 naive (catalog 480040) or total CD4 (catalog 480033) T cell MojoSort isolation kits (both from BioLegend), respectively, for adoptive transfer into Rag1–/–

(16) NFAT single-deficient murine T cells reduce the risk of aGvHD while controlling cytomegalovirus infectioniScienceFebruary 21, 2025Nadine Hundhausen, Snigdha Majumder, Yin Xiao, Sigrun S. Haeusl, Helen Goehler, Rishav Seal, Cristina M. Chiarolla, Andreas Rosenwald, Matthias Eyrich, Luka Cicin-Sain, et al.manufacturer’s instructions. CD4+ or CD8+ T cells were subsequently isolated using the negative Isolation kit (480033 or 480035, Biolegend) according to the manufacturer’s instructions.3 Allogenic hematopoietic stem cell transplantation

(17) Imaging of biphasic signalosomes constructed by checkpoint receptor 2B4 in conventional and chimeric antigen receptor-T cellsiScienceJanuary 17, 2025Ryohei Matsushima, Ei Wakamatsu, Hiroaki Machiyama, Wataru Nishi, Yosuke Yoshida, Tetsushi Nishikawa, Hiroko Toyota, Masae Furuhata, Hitoshi Nishijima, Arata Takeuchi, et al.assays,Critical commercial assays,Critical commercial assays ,, Mojo Mouse CD4 T Cells Isolation Kit,BioLegend,480033 Mojo Mouse CD8 T Cells Isolation Kit,BioLegend,480035 APC Annexin V Apoptosis Detection Kit with 7-AAD,BioLegend,640930 TNF-α

(18) NF-κB c-Rel is a critical regulator of TLR7-induced inflammation in psoriasisEBioMedicineDecember 1, 2024Liu AR, Sarkar N, Cress JD, de Jesus TJ, Vadlakonda A, Centore JT, Griffith AD, Rohr B, McCormick TS, Cooper KD, et al.freshly harvested spleens of WT C57BL6/J mice by magnetic sorting with MojoSort CD4 T cell Isolation Kit (480033, BioLegend). Purified T cells were cocultured with BMDCs at a 4:1 ratio in a 96 well plate. For Th17

(19) Inhibitory co-receptor Lag3 supports Foxp3+ regulatory T cell function by restraining Myc-dependent metabolic programmingImmunityNovember 1, 2024Dongkyun Kim, Giha Kim, Rongzhen Yu, Juyeun Lee, Sohee Kim, Mia R. Gleason, Kevin Qiu, Elena Montauti, Li Lily Wang, Deyu Fang, et al.commercial assays,Critical commercial assays MojoSort™ Mouse CD4 T Cell Isolation Kit,Biolegend,Cat # 480033 Mouse T cell nucleofector kit,Amaxa,Cat # VPA-1006 PureYield™ Plasmid Miniprep,Promega,Cat # A1223 PureLink™ HiPure Plasmid Filter Maxiprep Kit

(20) PD-L1 restrains PD-1+Nrp1lo Treg cells to suppress inflammation-driven colorectal tumorigenesisCell ReportsOctober 22, 2024Dakota B. Poschel, John D. Klement, Alyssa D. Merting, Chunwan Lu, Yang Zhao, Dafeng Yang, Wei Xiao, Huabin Zhu, Ponnala Rajeshwari, Michael Toscano, et al.,Vector Laboratories,H3301–250 BD Inracellular staining kit,BD Biosciences,555028 CD4 cell isolation kit MojoSort,Biolegend,480033 ImmunoCult Mouse Treg Differentiation Supplement,Stemcell Technologies,10957 Chromium Single Cell

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(1)

11/16/2020Relatively Simple Kit but not Always Enriched CD4s Meng SunFor some experiments, we require a pure CD4 population from the beginning therefore we use quick kits like this for enriching CD4s.
(1)

1/31/2020CD4 Enrichment of Cultured Splenocytes Using MojoSort Troy TrevinoThe MojoSort Mouse CD4 T cell isolation kit works very well to enrich for CD4 cells in a primary splenocyte culture. When used in tandem with a dead cell separation medium like Lympholyte-M, high ... Read Review
(1)

12/27/2019Pan CD4 T Cell Isolation Kit from Mouse Wei XuYou can use this kit to get acceptable yield and purity to isolate CD4 cells from mouse with reasonable price.
(1)

4/17/2018Isolating CD4 T Cells Matthew SmithI used this kit side by side with StemCell Technologies Mouse CD4 negative selection kit. Biolegend's product returned similar cell numbers and purity and is MUCH cheaper. The protocol is slightly ... Read Review
(1)

9/7/2017Isolation Of Mouse CD4 T Cell (Negative Selection) Jeeho LeeThe purpose of using this kit is to purify the "Untouched" CD4 T cells from murine spleen and lymph nodes. Isolated T cells were cultured in vitro or were transferred into mice for in vivo study.

Four Key Considerations When Performing Magnetic Cell Separation
February 02, 2016
The separation of specific cells within a complex cell mixture is a challenge that researchers face on a daily basis. Three major techniques have emerged as most widely used: centrifugation-based, fluorescence-activated ... read more

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MojoSort™ Mouse CD4 T Cell Isolation Kit from BioLegend

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Four Key Considerations When Performing Magnetic Cell Separation

MojoSort™ Mouse CD4 T Cell Isolation Kit from BioLegend

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