Biolegend
MojoSort™ Mouse CD4 T Cell Isolation Kit
480033
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The purpose of using this kit is to purify the "Untouched" CD4 T cells from murine spleen and lymph nodes. Isolated T cells were cultured in vitro or were transferred into mice for in vivo study.
In vitro culture/adoptive transfer into mice for in vivo study
Mouse spleen and lymph nodes
1. Total splenocytes and lymph node cells were prepared by grinding with 70um nylon mesh. 2. RBCs were removed by ACK lysis3. Add Ab cocktail and incubate for 15-20 min at 4 degrees 4. Add magnetic beads and incubate for 15-20 min at 4 degrees 5. Put the tube in the magnetic stand for 5 mins 6. Non-attached cells (CD4+) were taken
During the procedure, it is important to remove any debris or aggregates.
Purification was very good. It is similar with kits from other companies such as StemCell Technolgies, BD and Miltenyi Biotec
N/A
High quality with low cost
As other kits, the magnetic stand is required.
Overall, this is good enough to isolate highly pure CD4 cells. In addition, it is not expensive like the products of other vendors.