ROS-Glo H2O2 Assay from Promega

Product Specs
ItemROS-Glo H2O2 Assay
CompanyPromega
Price $354.00Supplier Page View Company Product Page
Catalog NumberG8820
Quantity10ml
Reactivitymammal
Sample Typecells in culture, tissue
TargetH2O2
Time20 minutes
Molecular Weight~104kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(81) Liver X Receptor Inverse Agonist GAC0001E5 Impedes Glutaminolysis and Disrupts Redox Homeostasis in Breast Cancer CellsBiomoleculesFebruary 10, 2023Premaratne A, Ho C, Basu S, Khan AF, Bawa-Khalfe T, Lin CY.(Corning Inc., Corning, NY, USA, #4517). Freshly prepared 50 μL of ROS-GloTM (Promega, Madison, WI, USA, #G8820) solution was added and incubated for 20 min. After 20 min, luminescence was read using the PerkinElmer

(82) Mechanism of cis-Nerolidol-Induced Bladder Carcinoma Cell DeathCancersFebruary 3, 2023Glumac M, Čikeš Čulić V, Marinović-Terzić I, Radan M.(H2O2-Glo) Intracellular ROS levels were measured using the ROS-Glo H2O2 assay (Promega Corp., Madison, WI, USA, G8820) following the manufacturer’s instructions. Briefly, TCCSUP cells were treated with nerolidol (50 mg/L)

(83) Loss of LKB1-NUAK1 signalling enhances NF-κB activity in a spheroid model of high-grade serous ovarian cancerScientific ReportsDecember 1, 2022Adrian Buensuceso, Jamie Lee Fritz, Olga Collins, Yudith Ramos Valdés, Matthew J. Borrelli, Gabriel E. DiMattia, Trevor G. ShepherdCayman Chemical (cat. 10010266; Ann Arbor, MI, USA). ROS-Glo H2O2 Assay was purchased from Promega (cat. G8820). Generation of STK11KO and NUAK1KO cell lines Generation of OVCAR8-STK11KO16 and OVCAR8-NUAK1KO20

(84) Efflux-linked accelerated evolution of antibiotic resistance at a population edgeMolecular CellNovember 9, 2022Souvik Bhattacharyya, Madhumita Bhattacharyya, Dylan M. Pfannenstiel, Anjan K. Nandi, YuneSahng Hwang, Khang Ho, Rasika M. HarsheyA1120 Measure-IT™ High-Sensitivity Nitrite Assay Kit,Thermo,Cat# M36051 ROS-Glo™ H2O2 Assay,Promega,Cat# G8820 LIVE/DEAD™ BacLight™ Bacterial Viability Kit,Thermo,Cat# L7007 CellROX™ Green Reagent,Thermo,Cat#

(85) Inhibition of autophagy with chloroquine dysregulates mitochondrial quality control and energetics in adipocytesArchives of pharmacal researchOctober 1, 2022Javaid HMA, Lim H, Shin S, Huh JY.measurement Hydrogen peroxide was measured using a commercially available ROS-Glo H2O2 assay kit (G8820, Promega; Madison, WI, USA) in accordance with the manufacturer’s instructions. Briefly, after the cells

(86) The Anti-Proliferative Lichen-Compound Protolichesterinic Acid Inhibits Oxidative Phosphorylation and Is Processed via the Mercapturic Pathway in Cancer CellsPlanta MedicaSeptember 1, 2022Freyr JÃ³hannsson, Paulina Cherek, Maonian Xu, Ã“ttar Rolfsson, Helga M. Ã–gmundsdÃ³ttirNADPH] and determine their ratio. H 2 O 2 was measured by using the ROS-Glo H 2 O 2 Assay kit (cat. no. G8820; Promega) according to the manufacturerʼs instructions. H 2 O 2 substrate solution was added for 2 h

(87) CCAAT/Enhancer-Binding Protein Delta Regulates Glioblastoma Survival through Catalase-Mediated Hydrogen Peroxide ClearanceOxid Med Cell LongevAugust 18, 2022Hong-Yi Lin, Sher-Wei Lim, Tsung-I Hsu, Wen-Bin Yang, Chi-Chen Huang, Yu-Ting Tsai, Wen-Chang Chang, Chiung-Yuan Koat a density of 5,000 cells per well. H2O2 levels were detected by using an ROS-Glo™ H2O2 assay kit (G8820, Promega) according to the manufacturer's instructions. Samples were incubated with H2O2 substrate solution

(88) Argonaute 2 modulates EGFR-RAS signaling to promote mutant HRAS and NRAS-driven malignanciesPNAS NexusJuly 1, 2022Siebenaler RF, Chugh S, Waninger JJ, Dommeti VL, Kenum C, Mody M, Gautam A, Patel N, Chu A, Bawa P, et al.seeded on a 96-well plate. Reactive oxygen species (ROS) were detected with the ROS-Glo H2O2 Assay Cat# G8820 (Promega) following the manufacturer's instructions, approximately 24 hours following adherence to dish. Molecular dynamics simulations

(89) Extracellular Signal-Regulated Kinase 1 Alone Is Dispensable for Hyperoxia-Mediated Alveolar and Pulmonary Vascular Simplification in Neonatal MiceAntioxidants (Basel, Switzerland)June 8, 2022Menon RT, Thapa S, Shrestha AK, Barrios R, Shivanna B.Generation The H2O2 production was quantified by the ROS-Glo™ H2O2 Assay (Promega, Madison, WI, USA; G8820) according to the manufacturer’s protocol. Briefly, cells grown on 96-well plates to 60–70% confluence

(90) A topological refactoring design strategy yields highly stable granulopoietic proteinsNature communicationsMay 26, 2022Skokowa J, Hernandez Alvarez B, Coles M, Ritter M, Nasri M, Haaf J, Aghaallaei N, Xu Y, Mir P, Krahl AC, et al.peroxide (H2O2), a reactive oxygen species (ROS), was measured with the ROS-Glo H2O2 Assay kit (Promega, #G8820) according to the manufacturer’s protocol. Quantitative analysis of NETosis formation Ex vivo generated

(91) Identification of Synergistic Drug Combinations to Target KRAS-Driven Chemoradioresistant Cancers Utilizing Tumoroid Models of Colorectal Adenocarcinoma and Recurrent GlioblastomaFrontiers in OncologyMay 18, 2022Kshama Gupta, Jeremy C. Jones, Virginea De Araujo Farias, Yuri Mackeyev, Pankaj K. Singh, Alfredo Quiñones-Hinojosa, Sunil KrishnanSpheroid growth and health were evaluated based on the increase in ROS levels (ROS-Glo™ H2O2 Assay, G8820, Promega), change in spheroid cell viability (CellTiter-Glo®, G7572, Promega), and induction of caspase

(92) Cannabinoid receptor 1 antagonist genistein attenuates marijuana-induced vascular inflammationCellMay 12, 2022Wei TT, Chandy M, Nishiga M, Zhang A, Kumar KK, Thomas D, Manhas A, Rhee S, Justesen JM, Chen IY, et al.2000,Thermo Fisher Scientific,11668019 CellTiter-Glo 2.0 Assay,Promega,G9241 ROS-Glo™ H2O2 Assay,Promega,G8820 GTPase-Glo™ Assay,Promega,V7681 "Cell ROX™ Deep Red Reagent, for oxidative stress detection",Thermo Fisher Scientific

(93) NADH oxidase of Mycoplasma hyopneumoniae functions as a potential mediator of virulenceBMC veterinary researchApril 2, 2022Hao F, Xie X, Feng Z, Chen R, Wei Y, Liu J, Xiong Q, Shao G, Lin J.independent replicates. Reactive oxygen species (ROS) were measured using a ROS-Glo™ H2O2 Assay (Cat No. G8820, Promega, Madison, WI, USA) according to the manufacturer’s instructions, and RLUs were recorded using

(94) DRG2 Depletion Promotes Endothelial Cell Senescence and Vascular Endothelial DysfunctionInternational journal of molecular sciencesMarch 6, 2022Le AN, Park SS, Le MX, Lee UH, Ko BK, Lim HR, Yu R, Choi SH, Lee BJ, Ham SY, et al.Shinjuku, Japan). 4.18. Measurement of ROS Levels H2O2 level was measured using a ROS-Glo H2O2 Assay (G8820, Promega) according to the protocol supplied by the manufacturer. Briefly, 1 × 104 cells/well were seeded

(95) Metabolic profiling of prostate cancer in skeletal microenvironments identifies G6PD as a key mediator of growth and survivalScience advancesFebruary 25, 2022Whitburn J, Rao SR, Morris EV, Tabata S, Hirayama A, Soga T, Edwards JR, Kaya Z, Palmer C, Hamdy FC, et al.to measure mitochondrial superoxide. Total ROS levels were measured using a ROS-Glo Kit (catalog no. G8820, Promega) and glutathione levels were measured using a GSH Glo kit (catalog no. V6911, Promega, Wisconsin,

(96) The diaryl-imidazopyridazine anti-plasmodial compound, MMV652103, exhibits anti-breast cancer activityEXCLI journalJanuary 1, 2022Neumann-Mufweba A, Kimani S, Khan SF, Chibale K, Prince S.stress in breast cancer cells, the levels of H2O2 were measured using the ROS-Glo™ H2O2 assay kit (G8820, Promega, Wisconsin, USA), according to the manufacturer's instructions. Briefly, cells (2500-4500 cells

(97) RARG variant predictive of doxorubicin-induced cardiotoxicity identifies a cardioprotective therapyCell stem cellDecember 2, 2021Magdy T, Jiang Z, Jouni M, Fonoudi H, Lyra-Leite D, Jung G, Romero-Tejeda M, Kuo HH, Fetterman KA, Gharib M, et al.Array,Illumina,WG-320-2101 CellTiter-Glo 2.0,Promega,G9242 Caspase 3/7-Glo,Promega,G8091 ROS-Glo H2O2,Promega,G8820 JC-10,Enzo Life Sciences,ENZ-52305 TruSeq RNA Library Prep Kit v2,Illumina,RS-122-2001 Deposited

(98) Complex II subunit SDHD is critical for cell growth and metabolism, which can be partially restored with a synthetic ubiquinone analogBMC Molecular and Cell BiologyDecember 1, 2021Aloka B. Bandara, Joshua C. Drake, David A. Browntriplicates. Generation of reactive oxygen species (ROS) was measured using ROS-Glo H2O2 Assay (Cat# G8820; Promega Corporation, Madison, WI). Apoptosis and necrosis were assessed using RealTime-Glo Annexin V Apoptosis and Necrosis Assay

(99) A novel role of KEAP1/PGAM5 complex: ROS sensor for inducing mitophagyRedox biologyDecember 1, 2021Zeb A, Choubey V, Gupta R, Kuum M, Safiulina D, Vaarmann A, Gogichaishvili N, Liiv M, Ilves I, Tämm K, et al.[70]. 4.9 ROS production To measure ROS induced by menadione, we used ROS-Glo™ H2O2 assay (Promega, G8820). PC6 cells were grown overnight on an opaque white 96 well plate. On the next day, the cells were treated

(100) Vitamin D-Mediated Anti-cancer Activity Involves Iron Homeostatic Balance Disruption and Oxidative Stress Induction in Breast CancerFrontiers in Cell and Developmental BiologyNovember 8, 2021Khuloud Bajbouj, Lina Sahnoon, Jasmin Shafarin, Abeer Al-Ali, Jibran Sualeh Muhammad, Asima Karim, Salman Y. Guraya, Mawieh HamadMeasurement Antioxidant related enzymes were measured using the ROS-Glo H2O2 Assay Kit (Promega, Cat No. G8820, United States) in both MDMBA-321 and MCF-7 cells, 5,500 cells/well were seeded using a 96-well plate

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