ROS-Glo H2O2 Assay from Promega

Product Specs
ItemROS-Glo H2O2 Assay
CompanyPromega
Price $354.00Supplier Page View Company Product Page
Catalog NumberG8820
Quantity10ml
Reactivitymammal
Sample Typecells in culture, tissue
TargetH2O2
Molecular Weight~104kDa
Time20 minutes

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(61) Antioxidants stimulate BACH1-dependent tumor angiogenesisThe Journal of clinical investigationOctober 16, 2023Wang T, Dong Y, Huang Z, Zhang G, Zhao Y, Yao H, Hu J, Tüksammel E, Cai H, Liang N, et al.and seeded in white 96-well plates (5,000 cells/well). ROS were measured with the ROS-Glo-H2O2 assay (G8820, Promega). The ratio of GSH/GSSG was determined with the GSH/GSSG-Glo assay (V6611, Promega). Fluorescence

(62) Establishment of a primary porcine retinal pigment epithelium monolayer to complement retinal ex vivo culturesSTAR protocolsSeptember 15, 2023Fietz A, Hurst J, Joachim SC, Schnichels S.commercial assays,Critical commercial assays ,, Promega’s ROS-Glo™ H2O2,"Promega, Walldorf, Germany",Cat#G8820 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, "GraphPad PRISM 9,

(63) P1357: MILESTONE (MODIFYING ELANE GOLDBERG–HOGNES BOX TO INHIBIT EXPRESSION), A UNIVERSAL, SAFE AND EFFECTIVE CRISPR/CAS9N-MEDIATED GENOME EDITING STRATEGY FOR ELANE RELATED SEVERE CONGENITAL NEUTROPENIAHemasphereAugust 8, 2023Masoud Nasri, Malte Ulrich Ritter, Perihan Mir, Benjamin Dannenmann, Masako Monika Kaufmann, Yun Xu, Natalia Borbaran-Bravo, Maksim Klimiankou, Claudia Lengerke, Cornelia Zeidler, et al.safety profiling. Reactive oxygen species (ROS) assay was measured by ROS-Glo Assay kit (Promega, #G8820). Assessment of phagocytosis kinetics was performed using the IncuCyte ZOOM system. Results: Using

(64) P1357: MILESTONE (MODIFYING ELANE GOLDBERG–HOGNES BOX TO INHIBIT EXPRESSION), A UNIVERSAL, SAFE AND EFFECTIVE CRISPR/CAS9N-MEDIATED GENOME EDITING STRATEGY FOR ELANE RELATED SEVERE CONGENITAL NEUTROPENIAHemasphereAugust 8, 2023Masoud Nasri, Malte Ulrich Ritter, Perihan Mir, Benjamin Dannenmann, Masako Monika Kaufmann, Yun Xu, Natalia Borbaran-Bravo, Maksim Klimiankou, Claudia Lengerke, Cornelia Zeidler, et al.safety profiling. Reactive oxygen species (ROS) assay was measured by ROS-Glo Assay kit (Promega, #G8820). Assessment of phagocytosis kinetics was performed using the IncuCyte ZOOM system. Results: Using

(65) Mechanisms of metabolic stress induced cell death of human oligodendrocytes: relevance for progressive multiple sclerosisActa neuropathologica communicationsJuly 5, 2023Fernandes MGF, Mohammadnia A, Pernin F, Schmitz-Gielsdorf LE, Hodgins C, Cui QL, Yaqubi M, Blain M, Hall J, Dudley R, et al.ATP and H2O2 were measured using Cell Titer-Glo 2.0 (Promega, G9242) and ROS-Glo H2O2 Assay (Promega G8820). Normalization to cell number was calculated using the ratio between the measures of ATP and H2O2 obtained

(66) TRIB1 regulates liver regeneration by antagonizing the NRF2-mediated antioxidant responseCell death & diseaseJune 24, 2023Sun X, Wang S, Miao X, Zeng S, Guo Y, Zhou A, Chen Y, Chen Y, Lv F, Fan Z, et al.double-blind fashion. Luminescence ROS assay ROS levels were measured by the ROS-Glo system (Promega, G8820) as previously described [28, 29]. Briefly, a luminescence substrate solution was added to and incubated

(67) Succinyl-CoA ligase ADP-forming subunit beta promotes stress granule assembly to regulate redox and drive cancer metastasisProceedings of the National Academy of Sciences of the United States of AmericaJune 6, 2023Boese AC, Kang J, Hwang JS, Kim J, Eun K, Malin CM, Magliocca KR, Pan C, Jin L, Kang S.Mitochondria isolation kit for cultured cells (89874) was obtained from Pierce. TransIT-LT1 (MIR2304) was from MirusBio. ROS-Glo H2O2 Assay (G8820) and Caspase-Glo 3/7 Assay Systems (G8091) were obtained from Promega. EZClick Global Protein Synthesis

(68) Myoglobin-derived iron causes wound enlargement and impaired regeneration in pressure injuries of muscleeLifeJune 2, 2023Nasir NJM, Heemskerk H, Jenkins J, Hamadee NH, Bunte R, Tucker-Kellogg L.measured for oxidative stress using the ROS-Glo H2O2 assay according to the manufacturer’s instructions (G8820, Promega). Luminescence was measured using a Tecan Spark M10 microplate reader (Tecan, Männedorf, Switzerland).

(69) Endoplasmic reticulum stress in the intestinal epithelium initiates purine metabolite synthesis and promotes Th17 cell differentiation in the gutImmunityMay 9, 2023Duan J, Matute JD, Unger LW, Hanley T, Schnell A, Lin X, Krupka N, Griebel P, Lambden C, Sit B, et al.,Miltenyi Biotec,Cat# 130-106-643 AmplexTM UltraRed,Invitrogen,Cat# A22188 ROS-Glo™ H2O2 Assay,Promega,Cat# G8820 RNeasy Plus Micro Kit,Qiagen,Cat# 74034 RNeasy Plus Mini Kit,Qiagen,Cat# 74134 Superscript™ IV VILO™ Master Mix

(70) Combating castration-resistant prostate cancer by co-targeting the epigenetic regulators EZH2 and HDACPLOS BiologyApril 27, 2023Amy E. Schade, Ryan Kuzmickas, Carrie L. Rodriguez, Kaia Mattioli, Miriam Enos, Alycia Gardner, Karen CichowskiTable. Reactive oxygen species assays ROS levels were detected using the ROSglo Assay Kit (Promega, cat # G8820) after 24 hours of combination treatment. ROS levels were normalized to DMSO-treated controls. ATP

(71) Treatment with Exogenously Added Catalase Alters CD8 T Cell Memory Differentiation and FunctionAdv Biol (Weinh)April 1, 2023Aksoylar HI, Patsoukis N.hydrogen peroxide concentration in the culture supernatant we used the ROS-Glo™ H2O2 assay kit (Promega, G8820) according to the manufacturer’s instructions. Briefly, 40 μl of culture supernatant, were mixed with

(72) PPAR-γ Activation Alleviates Osteoarthritis through Both the Nrf2/NLRP3 and PGC-1α/Δψ m Pathways by Inhibiting PyroptosisPPAR ResearchMarch 27, 2023Zhencheng Feng, Qiuxiang Huang, Xingliang Zhang, Pengfei Xu, Siming Li, Dongli Ma, Qingqi MengFisher Scientific) for detecting polyvinylidene fluoride (PVDF) membranes. ROS-GloTM H2O2 Assay kit (G8820, Promega). Nrf2 small interfering RNA (siRNA) (sense: GCUCAGAACUGUAGGAAAATT; antisense: UUUUCCUACAGUUCUGAGCTT)

(73) Dual Role of Mitogen-Activated Protein Kinase 8 Interacting Protein-1 in Inflammasome and Pancreatic β-Cell FunctionInternational Journal of Molecular SciencesMarch 5, 2023Rania Saeed, Abdul Khader Mohammed, Sarra E. Saleh, Mohammad M. Aboulwafa, Khaled M. Aboshanab, Jalal Taneerainstructions, the intracellular generation of ROS was detected using a ROS-Glo H2O2 assay kit (Cat #G8820, Promega, Madison, WI, USA). Briefly, the transfected and non-transfected INS-1 cells were treated with

(74) Expression Silencing of Mitogen-Activated Protein Kinase 8 Interacting Protein-1 Conferred Its Role in Pancreatic β-Cell Physiology and Insulin SecretionMetabolitesFebruary 20, 2023Saeed R, Mohammed AK, Saleh SE, Aboshanab KM, Aboulwafa MM, Taneera J.Oxygen Species (ROS) Measurements Intracellular ROS was detected using a ROS-Glo H2O2 assay kit (Cat #G8820, Promega, Madison, WI, USA). In brief, 48 h post-transfection, INS-1 cells were incubated with H2O2

(75) Liver X Receptor Inverse Agonist GAC0001E5 Impedes Glutaminolysis and Disrupts Redox Homeostasis in Breast Cancer CellsBiomoleculesFebruary 10, 2023Premaratne A, Ho C, Basu S, Khan AF, Bawa-Khalfe T, Lin CY.(Corning Inc., Corning, NY, USA, #4517). Freshly prepared 50 μL of ROS-GloTM (Promega, Madison, WI, USA, #G8820) solution was added and incubated for 20 min. After 20 min, luminescence was read using the PerkinElmer

(76) Influence of 2-Nitroimidazoles in the Response of FaDu Cells to Ionizing Radiation and Hypoxia/Reoxygenation StressAntioxidants (Basel, Switzerland)February 6, 2023Rashed FB, Kate WD, Fanta M, Wiebe LI, Kumar P, Weinfeld M.used as a readout of reactive oxygen species (ROS) and were analyzed using a ROS-Glo™ H2O2 assay kit (G8820, Promega, Madison, WI, USA). Briefly, FaDu cells seeded in 96-well plates were treated as indicated

(77) Mechanism of cis-Nerolidol-Induced Bladder Carcinoma Cell DeathCancersFebruary 3, 2023Glumac M, Čikeš Čulić V, Marinović-Terzić I, Radan M.(H2O2-Glo) Intracellular ROS levels were measured using the ROS-Glo H2O2 assay (Promega Corp., Madison, WI, USA, G8820) following the manufacturer’s instructions. Briefly, TCCSUP cells were treated with nerolidol (50 mg/L)

(78) Altered zinc homeostasis in a primary cell culture model of the retinal pigment epitheliumFrontiers in nutritionJanuary 1, 2023Álvarez-Barrios A, Álvarez L, Artime E, García M, Lengyel I, Pereiro R, González-Iglesias H.days after renewal of culture media, using the ROS-Glo™ H2O2 Assay purchased from Promega (Cat. No. G8820). H2O2 diffused from the cells to the culture media was quantified by the luminescent signals produced

(79) Loss of LKB1-NUAK1 signalling enhances NF-κB activity in a spheroid model of high-grade serous ovarian cancerScientific ReportsDecember 1, 2022Adrian Buensuceso, Jamie Lee Fritz, Olga Collins, Yudith Ramos Valdés, Matthew J. Borrelli, Gabriel E. DiMattia, Trevor G. ShepherdCayman Chemical (cat. 10010266; Ann Arbor, MI, USA). ROS-Glo H2O2 Assay was purchased from Promega (cat. G8820). Generation of STK11KO and NUAK1KO cell lines Generation of OVCAR8-STK11KO16 and OVCAR8-NUAK1KO20

(80) Efflux-linked accelerated evolution of antibiotic resistance at a population edgeMolecular CellNovember 9, 2022Souvik Bhattacharyya, Madhumita Bhattacharyya, Dylan M. Pfannenstiel, Anjan K. Nandi, YuneSahng Hwang, Khang Ho, Rasika M. HarsheyA1120 Measure-IT™ High-Sensitivity Nitrite Assay Kit,Thermo,Cat# M36051 ROS-Glo™ H2O2 Assay,Promega,Cat# G8820 LIVE/DEAD™ BacLight™ Bacterial Viability Kit,Thermo,Cat# L7007 CellROX™ Green Reagent,Thermo,Cat#

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