ROS-Glo H2O2 Assay from Promega

Product Specs
ItemROS-Glo H2O2 Assay
CompanyPromega
Price $354.00Supplier Page View Company Product Page
Catalog NumberG8820
Quantity10ml
Reactivitymammal
Sample Typecells in culture, tissue
TargetH2O2
Time20 minutes
Molecular Weight~104kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

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(22) The origin of hepatocellular carcinoma depends on metabolic zonationScienceJanuary 29, 2026Jason Guo, Roger Liang, Andrew Chung, Zhijie Li, Boyuan Li, Eric Chen, Lin Li, Jingjing Wang, Meng-Hsiung Hsieh, Ivy Xiangyi Fang, et al.using a fluorescent microscope. ROS detection To quantify ROS, the ROS-Glo H2O2 Assay Kit (Promega #G8820) was used according to the manufacturer’s instructions. Briefly, 20 mg of tissue was lysed with Passive Lysis 5X Buffer

(23) Baicalin Alleviates Chronic Restraint Stress-Induced Depression-like Behavior by Suppressing ROS/H2O2 Generation via a BDNF-Associated Mechanism in MiceAntioxidants (Basel)January 21, 2026Yu-Ning Teng, Tien-Wei Hsu, Wei-Hao Peng, Cheng-Chun Wu, Tian-Huei Chu, Yung-Kuo Lee, Ming Tatt Lee, Yu-Cheng Hoexperiments were performed according to the ROS-Glo™ H2O2 Assay manual (Promega, Madison, WI, USA, Cat. #G8820). Briefly, cells were seeded into a 96-well white plate at a density of 1 × 105 cells per well and incubated

(24) RAS/PI3K pathway mutations sensitise epithelial ovarian cancer cells to a PARP/NAMPT inhibitor combinationCommun BiolDecember 19, 2025Michael Gruet, Yitao Xu, Lyutong An, Yurui Ma, Cristina Balcells, Katie Tyson, Laila C. Evangelista, Sarah Spear, Yuewei Xu, Flora McKinney, et al.drug treatment. After 24- (human) or 48-h (ID8), H2O2 was measured using ROS-Glo H2O2 assay (Promega, G8820) according to the manufacturer’s manual. Briefly, 6-h before the endpoint is reached, 20 μL of combined

(25) Inhibiting translation elongation by reducing eIF5A activity induces feedback inhibition of initiation, limiting tumour cell proliferationNat CommunDecember 13, 2025Aristeidis P. Sfakianos, Rebecca M. Raven, Tom Smith, Xiao-Ming Sun, Thomas E. Mulroney, Mariavittoria Pizzinga, Veronica Dezi, Angela Rubio Tenor, Mark Stoneley, Cameron H. Cole, et al.described previously. ROS detection assay For ROS detection we used ROS-Glo™ H2O2 Assay (Promega, G8820), according to manufacturer’s protocol. Menadione (Supplementary Table 1) was used as a positive control. ATP

(26) Targeted Inhibition of ELANE Expression Using Adenine Base Editing to Treat Severe Congenital NeutropeniaMolecular Therapy Methods & Clinical DevelopmentDecember 11, 2025Betül Findik, Benjamin Dannenmann, Franka Bernhard, Masako Monika Kaufmann, Sandra Ammann, Sergey Kandabarau, Maksim Klimiankou, Fabian Mauch, Patrick Münzer, Oliver Borst, et al.a form of Reactive Oxygen Species (ROS), were measured using the ROS-Glo H2O2 assay kit (Promega, #G8820) on a GloMax multidetection system plate reader, following the manufacturer’s protocol. Phagocytosis

(27) Innate immune and metabolic signals induce mitochondria-dependent membrane lysis via mitoxyperiosisCellDecember 11, 2025Yaqiu Wang, Jianlin Lu, Alexandre F. Carisey, Sangappa B. Chadchan, Ha Won Lee, R.K. Subbarao Malireddi, Bhesh Raj Sharma, Nagakannan Pandian, Rebecca E. Tweedell, Gustavo Palacios, et al.9.9.4 (BD). Hydrogen peroxide (H2O2) measurement in cells was done with the ROS-Glo™ H2O2 Assay kit (G8820, Promega) following manufacturer’s instructions. Briefly, 1 × 105 cells were stimulated in each well

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(29) Coumarin-1,2,3-Triazole Conjugates as Molecular Scaffolds for the Selective Induction of ROS-Driven Apoptosis in Cancer Cells in the Development of Pyruvate Kinase M2 InhibitorsACS OmegaDecember 9, 2025Gabriel Ouverney, Amanda de Andrade Borges, Acácio Souza da Silva, Caroline Reis Santiago Paschoal, Paula Alvarez Abreu, Analice Gonçalves Rodrigues da Cruz, Mateus de Freitas Brito, Lucas Silva Abreu, Vitor Francisco Ferreira, Fernando de Carvalho da Silva, et al.of hydrogen peroxide (H2O2) using the ROS-Glo H2O2 assay kit (Promega Corporation, Madison, WI, USA; G8820). To carry out the experiment, 1 × 104 SCC-9 cells were plated per well in a 96-well plate, and after

(30) Asymmetric biphasic electric stimulation supports cardiac maturation and functionalityJournal of Tissue EngineeringNovember 28, 2025Antonio Sileo, Stefano Gabetti, Alp Can Gülan, Igor Cervenka, Chunyan Zhang, Alma Mingels, Giulia Milan, Diana Massai, Anna MarsanoSwitzerland). Reactive oxygen species (ROS) into the cell culture medium were quantified using the ROS-GLO H2O2 Assay (G8820, Promega, USA), following the manufacturer’s instructions. Luminescence was measured using a microplate

(31) NADPH Oxidase Isoform NOX-2 Deficiency Affects Mitochondrial Oxygen Consumption and Metabolic FlexibilityRedox BiologyNovember 26, 2025Kannathasan Thetchinamoorthy, Diana Wierzbicka, Emilia Waraksa-Zasada, Michalina Kazek, Adrian Konopko, Mariusz Z. Ratajczak, Magdalena KuciaHydrogen peroxide (H2O2) level (ROS glow assay luminescence) The ROS-Glo H2O2 AssayTM (Promega, Cat. No. G8820) was used to measure hydrogen peroxide levels following the manufacturer's instructions. Briefly, cells

(32) Role of Nitric Oxide and Nrf2 to Counteract Vascular Endothelial Dysfunction Induced by Periodontal Pathogens Using HUVECsCellsNovember 12, 2025Gunaraj Dhungana, Chethan Sampath, Vineeta Sharma, Olga Korolkova, Pandu R. Gangulaperoxide (H2O2) levels in culture supernatants were determined using the ROS-Glo™ H2O2 Assay (Cat. no. G8820, Promega, Madison, WI, USA). HUVECs were infected and treated as described above (Section 2.4). At each

(33) Non-canonical dihydrolipoyl transacetylase promotes chemotherapy resistance via mitochondrial tetrahydrofolate signalingNat CommunOctober 8, 2025Jung Seok Hwang, JiHoon Kang, Jaehyun Kim, Kiyoung Eun, Sophia West, Hannah E. Bacho, Vanessa Avalos, Sydney Shuff, Dong M. Shin, Nabil F. Saba, et al.(89874) was obtained from Pierce. MitoTracker Red CMXros (M7512) was from Invitrogen. ROS-Glo H2O2 Assay (G8820), NADP/NADPH-Glo Assay (G9081), and GSH/GSSG-Glo Assay (V6611) were procured from Promega. MtioSOX (M36008)

(34) Spinocerebellar Ataxia Type 1 (SCA1) Cell Models Display Widespread Mitochondrial and Extra-Nuclear AlterationsJ Mol NeurosciOctober 2, 2025Dane Ford-Roshon, Madison Dudek, Ada Glynn, Abigale Glasman, Jaden York, Emily Lawrence, Donna Nguyen, Lindsay Shinn, Georgia Berry, Lily Kendall, et al.long half-life, was measured in WT Daoy and ATXN1[82Q] cells using the ROS-Glo H2O2 Assay kit (Promega G8820). Harvested cells were plated at a concentration of 3000 Daoy cells or 5000 ATXN1[82Q] cells, to account

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