ROS-Glo H2O2 Assay from Promega

Product Specs
ItemROS-Glo H2O2 Assay
CompanyPromega
Price $354.00Supplier Page View Company Product Page
Catalog NumberG8820
Quantity10ml
Reactivitymammal
Sample Typecells in culture, tissue
TargetH2O2
Time20 minutes
Molecular Weight~104kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(41) IL-6-mediated tumorigenicity and antioxidant state in squamous cell carcinoma cells are driven by CD109 via stabilization of IL-6 receptor-alpha and activation of STAT3/NRF2 pathwayExp Hematol OncolMay 2, 2025Amani Hassan, Tenzin Kungyal, Shufeng Zhou, Meryem Blati, Kenneth Finnson, Nick Bertos, Nahid Golabi, Nader Sadeghi, Sampath Loganathan, Anie PhilipROS-Glo™ H2O2 assay ROS levels were measured using ROS-Glo™ H2O2 Assay kit from Promega (Promega; # G8820) according to manufacturer’s instructions. A431WT, SCC9 WT and their corresponding CD109 KO cells were

(42) Exploiting the therapeutic vulnerability of IDH-mutant gliomas with zotiraciclibiScienceApril 18, 2025Ying Pang, Qi Li, Zach Sergi, Guangyang Yu, Olga Kim, Peng Lu, Marina Chan, Xueyu Sang, Herui Wang, Alice Ranjan, et al.commercial assays,Critical commercial assays,Critical commercial assays ,, ROS-Glo H2O2 assay kit,Promega,G8820 Caspase-Glo 3/7 Assay Kit,Promega,G8091 Seahorse XF Cell Mito Stress Test Kit,Agilent,103015-100 Seahorse

(43) Signaling and transcriptional dynamics underlying early adaptation to oncogenic BRAF inhibitionCell SystemsApril 16, 2025Cameron T. Flower, Chunmei Liu, Hui-Yu Chuang, Xiaoyang Ye, Hanjun Cheng, James R. Heath, Wei Wei, Forest M. Whitehour prior to ROS quantitation. ROS levels were quantified by ROS-Glo H2O2 Assay (Promega, catalog #G8820) according to the manufacturer’s protocol. The luminescence signals were measured by the Synergy H4

(44) Chaperone-mediated autophagy regulates the metastatic state of mesenchymal tumorsEMBO Molecular MedicineMarch 7, 2025Xun Zhou, Eva Berenger, Yong Shi, Vera Shirokova, Elena Kochetkova, Tina Becirovic, Boxi Zhang, Vitaliy O Kaminskyy, Yashar Esmaeilian, Kayoko Hosaka, et al.Test Kit,Seahorse Bioscience,103010-100 G6PDH Assay Kit,Abcam,ab102529 ROS-Glo H2O2 assay,Promega,G8820 Software,Software,Software R (v 4.0.5),https://www.r-project.org/, tximport package (v 1.26.1),https://bioconductor.org/packages/release/bioc/html/tximport.html, clusterProfiler

(45) Calcium electroporation induces stress response through upregulation of HSP27, HSP70, aspartate β-hydroxylase, and CD133 in human colon cancer cellsBiol ResFebruary 21, 2025Anna Szewczyk, Nina Rembiałkowska, Jolanta Saczko, Małgorzata Daczewska, Vitalij Novickij, Julita Kulbackaviable cells relative to untreated controls. ROS level investigation The ROS-Glo™ H₂O₂ Assay (Promega, G8820) is a sensitive bioluminescent assay that directly measures cell culture’s reactive oxygen species (ROS)

(46) The AMD-associated genetic polymorphism CFH Y402H confers vulnerability to Hydroquinone-induced stress in iPSC-RPE cellsFront ImmunolFebruary 6, 2025Angela Armento, Inga Sonntag, Ana-Cristina Almansa-Garcia, Merve Sen, Sylvia Bolz, Blanca Arango-Gonzalez, Ellen Kilger, Ruchi Sharma, Kapil Bharti, Rosario Fernandez-Godino, et al.fluorescence and luminescence measurements. Levels of H2O2 were measure using the ROS-Glo H2O2 assay (G8820, Promega) and assay was performed according to manufacturer instructions. Briefly, cells were exposed

(47) RNF7‐Mediated ROS Targets Malignant Phenotype and Radiotherapy Sensitivity in Glioma With Different IDH1 GenotypesMolecular CarcinogenesisJanuary 9, 2025Yiran Tao, Zimin Shi, Xianyin Liang, Yuqian Zheng, Lirui Dai, Xiang Li, Zian Li, Wulong Liang, Gaojie Bai, Hao Li, et al.using the Reactive Oxygen Species Assay Kit (Beyotime, S0033S) or the ROS‐Glo H2O2 assay kit (Promega, G8820). Following the manufacturer's protocols, the treated samples were examined using a fluorescence microscope

(48) Pharmacological Vitamin C-induced high H2O2 generation mediates apoptotic cell death by caspase 3/7 activation in breast cancer tumor spheroidsJ Transl MedJanuary 8, 2025Ali Mussa, Mahasin Hamid, Khalid Hajissa, Ahmad Hafiz Murtadha, Mohammad A. I. Al-Hatamleh, Noor Fatmawati Mokhtar, Vuk Uskoković, Magdalena Plebanski, Rohimah Mohamud, Rosline HassanVit-C doses for 72 h, the cellular ROS was determined by performing the ROS-GLO™ H2O2 ASSAY (PROYY-G8820, Promega, USA) according to the protocol from the manufacturer. In short, from the H2O2 substrate solution

(49) Evaluating the Antibacterial Potential of Distinct Size Populations of Stabilized Zinc NanoparticlesACS Applied Materials & InterfacesJanuary 8, 2025Dinny Stevens, Amanda K. Charlton-Sevcik, W. Evan Braswell, Christie M. Sayesh exposure was measured through H2O2 production using the Luminescent ROS-Glo H2O2 assay (Promega, #G8820). The positive control for ROS production was 200 μM menadione (Thermo Fisher Scientific). Bacteria

(50) β-Lapachone promotes the recruitment and polarization of tumor-associated neutrophils (TANs) toward an antitumor (N1) phenotype in NQO1-positive cancersOncoImmunologyDecember 31, 2024Soumya Tumbath, Lingxiang Jiang, Xiaoguang Li, Taolan Zhang, Kashif Rafiq Zahid, Ye Zhao, Hao Zhou, Zhijun Yin, Tao Lu, Shu Jiang, et al.and anti-HMGB1 monoclonal antibodies were purchased from BioXCell (Lebanon, NH). ROS-GloTM assay kit (G8820) was obtained from Promega (Madison, WI). TACSTM Annexin V-FITC apoptosis detection kit was purchased

(51) Efficacy of glutathione inhibitor eprenetapopt against the vulnerability of glutathione metabolism in SMARCA4-, SMARCB1- and PBRM1-deficient cancer cellsScientific ReportsDecember 28, 2024Mariko Sasaki, Hideaki OgiwaraGSH/GSSG-Glo Assay (Promega, V6611), the GSH-Glo Assay (Promega, V6911), the ROS-Glo H2O2 Assay (Promega, G8820), and the Caspase-Glo 3/7 Assay (Promega, G8091), respectively. To measure levels of GSH/GSSG, ROS,

(52) Reactive oxygen species control protein degradation at the mitochondrial import gateMolecular cellDecember 5, 2024McMinimy R, Manford AG, Gee CL, Chandrasekhar S, Mousa GA, Chuang J, Phu L, Shih KY, Rose CM, Kuriyan J, et al.commercial assays,Critical commercial assays,Critical commercial assays ROS-Glo H2O2 Assay,Promega,Cat#G8820 Ionic Detergent Compatibility Reagent,Thermo Fisher Scientific,Cat#22663 Pierce 660nm Protein Assay Reagent

(53) A Novel Class of FKBP12 Ligands Rescues Premature Aging Phenotypes Associated with Myotonic Dystrophy Type 1CellsNovember 22, 2024Mikel García-Puga, Gorka Gerenu, Ariadna Bargiela, Jorge Espinosa-Espinosa, Laura Mosqueira-Martín, Maialen Sagartzazu-Aizpurua, Jesús M. Aizpurua, Ainara Vallejo-Illarramendi, Rubén Artero, Adolfo López de Munain, et al.and, after 14–16 h, was treated with 0.1 µM MP compounds for 72 h. Afterwards, ROS-Glo H2O2 Assay (G8820, Promega, Madison, WI, USA) was performed according to the manufacturer’s instructions to measure H2O2

(54) CCL2-mediated endothelial injury drives cardiac dysfunction in long COVIDNature Cardiovascular ResearchOctober 14, 2024Dilip Thomas, Chikage Noishiki, Sadhana Gaddam, David Wu, Amit Manhas, Yu Liu, Dipti Tripathi, Nimish Kathale, Shaunak S. Adkar, Jaishree Garhyan, et al.were used. Production of ROS ROS levels in COs were determined using ROS-Glo H2O2 Assay (Promega, G8820) as per the manufacturer’s instructions. In brief, equal proportions of COs that were treated with vehicle

(55) Anti-Proliferative and Anti-Migratory Activity of Licorice Extract and Glycyrrhetinic Acid on Papillary Thyroid Cancer Cell CulturesInternational Journal of Molecular SciencesOctober 8, 2024Jacopo Manso, Simona Censi, Maria Chiara Pedron, Loris Bertazza, Alberto Mondin, Edoardo Ruggeri, Susi Barollo, Chiara Sabbadin, Isabella Merante Boschin, Decio Armanini, et al.made through the Reactive Oxygen Species (ROS)-Glo™ H2O2 Assay (Promega, Madison, WI, USA; Cat. No. G8820) following the manufacturer’s instructions. Briefly, cells were seeded and treated for 1 h, as described

(56) Cytotoxicity, Proapoptotic Activity and Drug-like Potential of Quercetin and Kaempferol in Glioblastoma Cells: Preclinical InsightsInternational Journal of Molecular SciencesOctober 5, 2024Magdalena Kusaczuk, Elena Tovar-Ambel, Paola Martín-Cabrera, Mar Lorente, Nélida Salvador-Tormo, Agnieszka Mikłosz, Adrian Chabowski, Guillermo Velasco, Monika NaumowiczCellTiter-Glo luminescent cell-viability assay (G7570), the Caspase-Glo 3/7 assay (G8981), the Caspase-Glo 9 assay, and the ROS-Glo H2O2 system (G8820) were purchased from Promega (Fitchburg, WI, USA). The Protease/Phosphatase Inhibitor Cocktail (#5872S),

(57) Oncometabolite 2-hydroxyglutarate suppresses basal protein levels of DNA polymerase beta that enhances alkylating agent and PARG inhibition induced cytotoxicityDNA Repair (Amst)August 1, 2024Kate M. Saville, Rasha Q. Al-Rahahleh, Aisha H. Siddiqui, Morgan E. Andrews, Wynand P. Roos, Christopher A. Koczor, Joel F. Andrews, Faisal Hayat, Marie E. Migaud, Robert W. Sobol0, 1, 2, 4, 6, or 8 hours. ROS was determined using the Promega ROS-Glo hydrogen peroxide kit (Cat# G8820) and was carried out according to the manufacturer’s instructions. All analyses were performed in triplicate

(58) Inhibition of PHLDA3 expression in human superoxide dismutase 1-mutant amyotrophic lateral sclerosis astrocytes protects against neurotoxicityBrain CommunicationsJuly 25, 2024Kornélia Szebényi, Ingrid Vargová, Veselina Petrova, Jana Turečková, George M Gibbons, Monika Řehořová, Mai Abdelgawad, Alexandra Sándor, Dana Marekova, Jessica C F Kwok, et al.transfected astrocytes and NeuN+ neurons. The reactive oxygen species (ROS)-Glo H2O2 assay (Promega, G8820) was used to detect ROS levels in astrocyte-conditioned media (ACM), indicated by the H2O2 luminescence

(59) S100A1’s single cysteine is an indispensable redox switch for the protection against diastolic calcium waves in cardiomyocytesAmerican Journal of Physiology-Heart and Circulatory PhysiologyJuly 1, 2024Andreas Seitz, Martin Busch, Jasmin Kroemer, Andrea Schneider, Stephanie Simon, Andreas Jungmann, Hugo A. Katus, Patrick Most, Julia RitterhoffBiotin-maleimide (B1267, Sigma-Aldrich), N-ethylmaleimide (E3876, Sigma-Aldrich), ROS-Glo H2O2 Assay (Promega, G8820). All other chemicals were obtained from Sigma-Aldrich, Germany, if not indicated specifically. Generation

(60) β-Lapachone promotes the recruitment and polarization of tumor-associated neutrophils (TANs) toward an antitumor (N1) phenotype in NQO1-positive cancersOncoimmunologyJune 4, 2024Soumya Tumbath, Lingxiang Jiang, Xiaoguang Li, Taolan Zhang, Kashif Rafiq Zahid, Ye Zhao, Hao Zhou, Zhijun Yin, Tao Lu, Shu Jiang, et al.and anti-HMGB1 monoclonal antibodies were purchased from BioXCell (Lebanon, NH). ROS-GloTM assay kit (G8820) was obtained from Promega (Madison, WI). TACSTM Annexin V-FITC apoptosis detection kit was purchased

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