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Assay Kit
TruSeq Stranded Total RNA Library Prep Gold (48 Samples)
TruSeq Stranded Total RNA Library Prep Gold (48 Samples) from Illumina
Be the first to write a review!
Citations:
(102)
Supplier Page
Supplier Page from
Illumina for
TruSeq Stranded Total RNA Library Prep Gold (48 Samples)
Description
Whole-transcriptome stranded RNA-Seq with Ribo-Zero Gold rRNA depletion, also removes mitochondrial rRNA; 48-sample format
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
TruSeq Stranded Total RNA Library Prep Gold (48 Samples)
Company
Illumina
Price
Supplier Page
View Company Product Page
Catalog Number
20020598
Applications
Total RNA-Seq, Whole-transcriptome sequencing
Reactivity
Human, Mouse, Rat
Type
RNA Library Prep Kit
Quantity
48 Samples
Add to Compare List
Illumina
5200 Illumina Way
San Diego
,
CA
92122
United States
Phone
: 858.202.4500
Company Profile
Website:
http://illumina.com
Citations (102)
(81)
NCK-associated protein 1 like (nckap1l) minor splice variant regulates intrahepatic biliary network morphogenesis
PLoS genetics
March 1, 2021
Ghaffari K, Pierce LX, Roufaeil M, Gibson I, Tae K, Sahoo S, Cantrell JR, Andersson O, Lau J, Sakaguchi TF.
for next-generation sequencing was synthesized by using the TruSeq standard total RNA kit (
Illumina
,
20020598
) according to the manufacturer’s instructions. Using an Illumina HighSeq 2500, a total of 11.5 and 10.5
(82)
IL-22 receptor signaling in Paneth cells is critical for their maturation, microbiota colonization, Th17-related immune responses, and anti-Salmonella immunity
Mucosal immunology
March 1, 2021
Gaudino SJ, Beaupre M, Lin X, Joshi P, Rathi S, McLaughlin PA, Kempen C, Mehta N, Eskiocak O, Yueh B,
et al
.
Reference Guide (Illumina Document #1000000040499v00, TruSeq Stranded Total RNA Gold
Illumina
Kit #
20020598
). Final cDNA libraries were quantitated using
Qubit dsDNA BR assay kit
(
Thermo Fisher Scientific
: Guide
(83)
TCF7L2 silencing results in altered gene expression patterns accompanied by local genomic reorganization
Neoplasia (New York, N.Y.)
February 1, 2021
Brown MA, Dotson GA, Ronquist S, Emons G, Rajapakse I, Ried T.
by the CCR
Sequencing
Facility using the TruSeq Stranded Total
RNA Library Prep
Gold kit (
Illumina
;
20020598
). Ribosomal RNA (rRNA) was removed using biotinylated, target-specific oligos combined with Ribo-Zero
(84)
A Role for the Mre11-Rad50-Xrs2 Complex in Gene Expression and Chromosome Organization
Molecular cell
January 7, 2021
Forey R, Barthe A, Tittel-Elmer M, Wery M, Barrault MB, Ducrot C, Seeber A, Krietenstein N, Szachnowski U, Skrzypczak M,
et al
.
24:1,Sigma,Cat#C0549–1PT
Superscript IV reverse transcriptase
,
Life Technologies
,Cat#
18090050
TruSeq stranded total RNA,
Illumina
,Cat#
20020598
Random Hexamers,
Life Technologies
,Cat#
N8080127
100mM
dNTP set
,
Life Technologies
,Cat#10297–018
RiboMinus Eukaryote Kit for RNA-seq
(85)
Protocol for Disome Profiling to Survey Ribosome Collision in Humans and Zebrafish
STAR Protocols
December 30, 2020
Mari Mito, Yuichiro Mishima, Shintaro Iwasaki
samples.Note: Ribo-Zero Kit accompanied by TruSeq Stranded Total
RNA Library Prep
Gold (
Illumina
, cat. no.
20020598
) can be used.Pause Point: Samples may be stored at −20°C or −80°C for several months. Reverse Transcription Timing:
(86)
Key Parameters of Tumor Epitope Immunogenicity Revealed Through a Consortium Approach Improve Neoantigen Prediction
Cell
October 29, 2020
Wells DK, van Buuren MM, Dang KK, Hubbard-Lucey VM, Sheehan KCF, Campbell KM, Lamb A, Ward JP, Sidney J, Blazquez AB,
et al
.
Library Kit (KAPA Biosystems),Roche,7961901001 TruSeq Stranded Total RNA with RiboZero Plus,
Illumina
,
20020598
Deposited Data,,
Whole exome sequencing
data (
melanoma
cohort),"Liu et al., 2019",phs000452.v3.p1 Transciptome
(87)
Pioglitazone Ameliorates Gentamicin Ototoxicity by Affecting the TLR and STAT Pathways in the Early Postnatal Organ of Corti
Frontiers in Cellular Neuroscience
October 29, 2020
Marijana Sekulic-Jablanovic, Matthew B. Wright, Vesna Petkovic, Daniel Bodmer
preparation was performed with 200 ng total RNA using the TruSeq Stranded Total
RNA Library Prep
Gold (Cat#
20020598
,
Illumina
, San Diego, CA, USA). Libraries were quality checked on a Fragment Analyzer (Advanced Analytical,
(88)
Genome-wide chromatin accessibility is restricted by ANP32E
Nature communications
October 8, 2020
Murphy KE, Meng FW, Makowski CE, Murphy PJ.
methods.
RNA-Seq
libraries were prepared using the TruSeq Stranded Total RNA Library Prep Kit (cat:
20020598
), according to the standard methods, and total RNA was purified using the
Zymo Research
Direct-zol RNA Miniprep
(89)
Alteration of genome folding via contact domain boundary insertion
Nature Genetics
October 1, 2020
Di Zhang, Peng Huang, Malini Sharma, Cheryl A. Keller, Belinda Giardine, Haoyue Zhang, Thomas G. Gilgenast, Jennifer E. Phillips-Cremins, Ross C. Hardison, Gerd A. Blobel
CRISPR
-edited
clones
, as the ScriptSeqv2 Complete Kit was discontinued, TruSeq Stranded Total RNA (
Illumina
:
20020598
) was used per manufacturer’s instructions, which relies on a dUTP-based second strand synthesis to preserve
(90)
Anticancer Imidazoacridinone C-1311 is Effective in Androgen-Dependent and Androgen-Independent Prostate Cancer Cells
Biomedicines
August 19, 2020
Niemira M, Borowa-Mazgaj B, Bader SB, Moszyńska A, Ratajewski M, Karaś K, Kwaśniewski M, Krętowski A, Mazerska Z, Hammond EM,
et al
.
an RNA integrity number (RIN) > 8, using the Illumina TruSeq® Stranded Total RNA Library Prep Kit (#
20020598
,
Illumina
, San Diego, CA, USA). Indexed libraries were pooled, clustered with use the cBot, and sequenced on
(91)
Single-cell analyses identify dysfunctional CD16(+) CD8 T cells in smokers
Cell reports. Medicine
July 21, 2020
Martos SN, Campbell MR, Lozoya OA, Wang X, Bennett BD, Thompson IJB, Wan M, Pittman GS, Bell DA.
MethylationEPIC BeadChip Kit,Illumina,Cat# WG-317-1003 TruSeq Stranded Total RNA Gold,
Illumina
,Cat#
20020598
,, Deposited Data,Deposited Data,Deposited Data ,, Single-cell RNA sequencing,This paper,GEO: GSE138867 Bulk
(92)
Feeding-dependent VIP neuron–ILC3 circuit regulates the intestinal barrier
Nature
March 26, 2020
Jhimmy Talbot, Paul Hahn, Lina Kroehling, Henry Nguyen, Dayi Li, Dan R. Littman
libraries for CCR6+ ILC3 were prepared with the TruSeq Stranded Total
RNA Library Prep
Gold Kit (
Illumina
,
20020598
). The
sequencing
was performed using Illumina NextSeq instrument (Ilumina, San Diego, USA) using 150bp,
(93)
Xenosiderophore Utilization Promotes Bacteroides thetaiotaomicron Resilience during Colitis
Cell Host & Microbe
March 1, 2020
Wenhan Zhu et al.
PowerMicrobiome Kit,
Qiagen
,Cat#
26000-50
TruSeq Stranded Total RNA Library Prep Kit,
Illumina
,Cat#
20020598
RNAlater™ Stabilization Solution
,
Thermo Fisher
,Cat#
AM7020
TOPO
cloning
kit,
Invitrogen
,Cat#
K457502
Gibson Assembly Cloning Kit
(94)
Molecular Signature of Subtypes of Non-Small-Cell Lung Cancer by Large-Scale Transcriptional Profiling: Identification of Key Modules and Genes by Weighted Gene Co-Expression Network Analysis (WGCNA)
Cancers (Basel)
December 21, 2019
Magdalena Niemira, Francois Collin, Anna Szalkowska, Agnieszka Bielska, Karolina Chwialkowska, Joanna Reszec, Jacek Niklinski, Miroslaw Kwasniewski, Adam Kretowski
RNA integrity number (RIN) >8, and using the Illumina TruSeq® Stranded Total
RNA Library Prep
Gold (#
20020598
,
Illumina
, San Diego, CA, USA). Indexed libraries were pooled, clustered with the use of the cBot, and
(95)
Diversification of the Caenorhabditis heat shock response by Helitron transposable elements
eLife
December 11, 2019
Jacob M Garrigues, Brian V Tsu, Matthew D Daugherty, Amy E Pasquinelli
expression analysis
of
RNA-seq
Software, algorithm Delly v0.8.1 Rausch et al., 2012 Genome comparisons
Illumina
:
20020598
Continued on next page Garrigues et al. eLife 2019;8:e51139. DOI: https://doi.org/10.7554/eLife.51139 16
(96)
Diversification of the Caenorhabditis heat shock response by Helitron transposable elements
Elife
December 11, 2019
Jacob M Garrigues, Brian V Tsu, Matthew D Daugherty, Amy E Pasquinelli
strain
Commercial
assay
or kit,Illumina TruSeq Stranded Total
RNA Library Prep
Gold Kit,Illumina,
Illumina
:
20020598
, "Software, algorithm",FigTree v1.4.4,http://
tree
.bio.ed.ac.uk/software/figtree/,, "Software, algorithm",Bowtie2
(97)
Genetic Control of Expression and Splicing in Developing Human Brain Informs Disease Mechanisms
Cell
October 17, 2019
Rebecca L. Walker, Gokul Ramaswami, Christopher Hartl, Nicholas Mancuso, Michael J. Gandal, Luis de la Torre-Ubieta, Bogdan Pasaniuc, Jason L. Stein, Daniel H. Geschwind
217004
DNeasy Blood and Tissue Kit,
QIAGEN
,Cat#
69506
Stranded TruSeq kit with Ribozero Gold,
Illumina
,Cat#
20020598
,, Deposited Data,Deposited Data,Deposited Data ,, Raw and analyzed data:
RNA-seq
,This paper,dbGaP:
(98)
An improved method for circular RNA purification using RNase R that efficiently removes linear RNAs containing G-quadruplexes or structured 3′ ends
Nucleic Acids Research
September 19, 2019
Mei-Sheng Xiao, Jeremy E Wilusz
for
RNA-seq
library preparation using the TruSeq Stranded Total
RNA Library Prep
Gold kit (
Illumina
20020598
) following the manufacturer's instructions except that a 1/100 dilution of the ERCC Spike-In Mix (
Thermo Fisher Scientific
(99)
NF-Y controls fidelity of transcription initiation at gene promoters through maintenance of the nucleosome-depleted region
Nature communications
July 11, 2019
Oldfield AJ, Henriques T, Kumar D, Burkholder AB, Cinghu S, Paulet D, Bennett BD, Yang P, Scruggs BS, Lavender CA,
et al
.
(
Thermo Scientific
,
18068015
) and stranded libraries were prepared using the TruSeq stranded RNA kit (
Illumina
,
20020598
) with RiboZero depletion (Gold kit; Illumina, MRZG12324).
RNA-Seq
data analysis Reads were mapped
(100)
Fc Characteristics Mediate Selective Placental Transfer of IgG in HIV-Infected Women
Cell
June 13, 2019
David R. Martinez, Youyi Fong, Shuk Hang Li, Fang Yang, Madeleine F. Jennewein, Joshua A. Weiner, Erin A. Harrell, Jesse F. Mangold, Ria Goswami, George R. Seage,
et al.
74104
RNA 6000 Nano Kit,
Agilent
,Cat#
5067–1511
TruSeq Stranded Total
RNA Library Prep
Gold,
Illumina
,Cat#
20020598
Deposited Data,Deposited Data,Deposited Data Placental RNaseq dataset,NCBI GEO (
Gene Expression
Omnibus),GEO:
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TruSeq Stranded Total RNA Library Prep Gold (48 Samples) from Illumina
Supplier Page
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