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Assay Kit
TruSeq Stranded Total RNA Library Prep Gold (48 Samples)
TruSeq Stranded Total RNA Library Prep Gold (48 Samples) from Illumina
Be the first to write a review!
Citations:
(102)
Supplier Page
Supplier Page from
Illumina for
TruSeq Stranded Total RNA Library Prep Gold (48 Samples)
Description
Whole-transcriptome stranded RNA-Seq with Ribo-Zero Gold rRNA depletion, also removes mitochondrial rRNA; 48-sample format
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
TruSeq Stranded Total RNA Library Prep Gold (48 Samples)
Company
Illumina
Price
Supplier Page
View Company Product Page
Catalog Number
20020598
Applications
Total RNA-Seq, Whole-transcriptome sequencing
Reactivity
Human, Mouse, Rat
Type
RNA Library Prep Kit
Quantity
48 Samples
Add to Compare List
Illumina
5200 Illumina Way
San Diego
,
CA
92122
United States
Phone
: 858.202.4500
Company Profile
Website:
http://illumina.com
Citations (102)
(61)
Molecular signature of postmortem lung tissue from COVID-19 patients suggests distinct trajectories driving mortality
Disease Models & Mechanisms
May 1, 2022
Anshul Budhraja, Anubhav Basu, Atish Gheware, Dasari Abhilash, Seesandra Rajagopala, Suman Pakala, Madhuresh Sumit, Animesh Ray, Arulselvi Subramaniam, Purva Mathur,
et al.
depletion, using TruSeq Stranded Total RNA Gold kit as per the manufacturer's instructions (
Illumina
,
20020598
). The libraries were then sequenced on an Illumina NovaSeq6000 platform with 2×150 base pair reads (details
(62)
HNF4A
and
GATA6
Loss Reveals Therapeutically Actionable Subtypes in Pancreatic Cancer
SSRN Electronic Journal
March 31, 2022
Holly Brunton et al.
kit. Ribosomal depletion step was performed on 500 ng of total RNA using Ribo- Zero Gold (
Illumina
,
20020598
and 20020492) followed by a 8 min heat fragmentation step aimed at producing libraries with an insert
(63)
Travel ban effects on SARS-CoV-2 transmission lineages in the UAE as inferred by genomic epidemiology
PLOS ONE
March 2, 2022
Andreas Henschel et al.
preparation Libraries were prepared using the
Illumina
TruSeq Stranded Total RNA Ribo Zero Gold (Catalog #
20020598
) kit according to the manufacturer’s protocol with recommended RNA concentration of 200 ng/ul as the
(64)
Medications Activating Tubular Fatty Acid Oxidation Enhance the Protective Effects of Roux-en-Y Gastric Bypass Surgery in a Rat Model of Early Diabetic Kidney Disease
Front Endocrinol (Lausanne)
January 26, 2022
William P. Martin et al.
RNA library preparation
was carried out using the TruSeq Stranded Total
RNA Library Prep
Gold® kit (Cat. No.
20020598
,
Illumina
). Libraries were sequenced on the Illumina NovaSeq 6000® platform in a paired-end fashion
(65)
Foxd3 controls heterochromatin-mediated repression of repeat elements and 2-cell state transcription
EMBO reports
December 6, 2021
Puri D, Koschorz B, Engist B, Onishi-Seebacher M, Ryan D, Soujanya M, Montavon T.
(
Qiagen
,
74204
). Libraries were prepared using the TruSeq Stranded Total
RNA Library Prep
Gold (
Illumina
,
20020598
) following Illumina protocols. The libraries were sequenced on a HiSeq2500 Illumina platform using a
(66)
Integrated whole transcriptome and small RNA analysis revealed multiple regulatory networks in colorectal cancer
Scientific Reports
December 1, 2021
Hibah Shaath, Salman M. Toor, Mohamed Abu Nada, Eyad Elkord, Nehad M. Alajez
stranded total
RNA library preparation
The TruSeq Stranded Total
RNA Library Prep
Gold kit (Cat #:
20020598
) from
Illumina
was used for total
RNA library preparation
following the manufacturer’s protocol. Briefly,
(67)
Transcriptional alterations of protein coding and noncoding RNAs in triple negative breast cancer in response to DNA methyltransferases inhibition
Cancer Cell International
December 1, 2021
Ramesh Elango, Radhakrishnan Vishnubalaji, Hibah Shaath, Nehad M. Alajez
as input for the library preparation using TruSeq Stranded Total
RNA Library Prep
Gold kit (Cat #:
20020598
) from
Illumina
following the manufacturer’s protocol. Briefly, 500 ng of total RNA was subjected to
(68)
LINE retrotransposons characterize mammalian tissue-specific and evolutionarily dynamic regulatory regions
Genome Biology
December 1, 2021
Maša Roller, Ericca Stamper, Diego Villar, Osagie Izuogu, Fergal Martin, Aisling M. Redmond, Raghavendra Ramachanderan, Louise Harewood, Duncan T. Odom, Paul Flicek
Illumina sequencing
libraries were prepared using the Illumina TruSeq Stranded Total RNA with Ribo Gold kit (
20020598
) with
Illumina
RNA UD Indexes (20020492) according to the manufacturer’s protocol. Samples were run
(69)
An integrated multi-omic analysis of iPSC-derived motor neurons from C9ORF72 ALS patients
iScience
November 19, 2021
NeuroLINCS Consortium, Li J, Lim RG, Kaye JA, Dardov V, Coyne AN, Wu J, Milani P, Cheng A, Thompson TG,
et al
.
RNeasy mini kit,
Qiagen
,
74104
Ribo-Zero Gold rRNA depletion and Truseq Stranded total RNA kit,
Illumina
,
20020598
Biognosys iRT mixture,Biognosys,Ki-3002-2 Expedeon FASP protocol,
Abcam
,
ab270519
BCA
assay
,
Pierce
,
23227
Nextera
(70)
Collective regulation of chromatin modifications predicts replication timing during cell cycle
Cell Reports
October 5, 2021
Capucine Van Rechem, Fei Ji, Damayanti Chakraborty, Joshua C. Black, Ruslan I. Sadreyev, Johnathan R. Whetstine
assays
miRNeasy Mini Kit
,
QIAGEN
,Cat#
217004
TruSeq Stranded Total
RNA Library Prep
Gold,
Illumina
,Cat#
20020598
TruSeq ChIP Library Preparation Kit Set A,Illumina,Cat# IP-202–1012 TruSeq ChIP Library Preparation
(71)
Transcriptional signatures in iPSC-derived neurons are reproducible across labs when differentiation protocols are closely matched
Stem cell research
October 1, 2021
Reed X, Cobb MM, Skinbinski G, Roosen D, Kaganovich A, Ding J, Finkbeiner S, Cookson MR.
TruSeq Stranded Total RNA Sample Prep LS Protocol with rRNA depletion using Ribo Zero Gold (
Illumina
,
20020598
). Library size was checked using the Bioanalyzer DNA 1000 kit (Aglient, 5067–1504) and each library
(72)
The avian W chromosome is a refugium for endogenous retroviruses with likely effects on female-biased mutational load and genetic incompatibilities
Philosophical Transactions of the Royal Society B
September 13, 2021
Valentina Peona, Octavio M. Palacios-Gimenez, Julie Blommaert, Jing Liu, Tri Haryoko, Knud A. Jønsson, Martin Irestedt, Qi Zhou, Patric Jern, Alexander Suh
TruSeq stranded total library preparation kit with RiboZero Gold treatment (
Illumina, Inc
., cat no.
20020598
/9). Paired-reads (150 bp) were sequenced on the NovaSeq SP flowcell (Illumina, Inc.). For
zebra
finch
,
(73)
Ribo-ODDR: oligo design pipeline for experiment-specific rRNA depletion in Ribo-seq
Bioinformatics (Oxford, England)
September 9, 2021
Alkan F, Silva J, Pintó Barberà E, Faller WJ.
is performed according to manufacturer’s instructions when using the RiboZero (
Illumina
, catalog no.
20020598
) and RiboCop (Lexogen, catalog no. 037) kits. For depletion with custom oligos, samples were incubated
(74)
Complete loss of H3K9 methylation dissolves mouse heterochromatin organization
Nature communications
July 16, 2021
Montavon T, Shukeir N, Erikson G, Engist B, Onishi-Seebacher M, Ryan D, Musa Y, Mittler G, Meyer AG, Genoud C,
et al
.
Total RNA libraries were prepared using the TruSeq Stranded Total
RNA Library Prep
Gold (
Illumina
,
20020598
). Paired-end, 75 bp reads were generated with Illumina HiSeq3000 sequencer with a depth of 50 million
(75)
The Transcriptome of Paired Major and Minor Salivary Gland Tissue in Patients With Primary Sjögren’s Syndrome
Frontiers in Immunology
July 6, 2021
Gwenny M. Verstappen, Lu Gao, Sarah Pringle, Erlin A. Haacke, Bert van der Vegt, Silvia C. Liefers, Vishal Patel, Yanhua Hu, Sumanta Mukherjee, Julie Carman,
et al.
DNA library preparation
was performed by using TruSeq Stranded Total
RNA Library Prep
Gold (
Illumina
, USA, Cat#
20020598
), following manufacturer’s recommendations. Prepared libraries were sequenced on a HiSeq 2500 System
(76)
Dual-Specificity Phosphatase 9 Regulates Cellular Proliferation and Predicts Recurrence After Surgery in Hepatocellular Carcinoma
Hepatology communications
July 1, 2021
Chen K, Gorgen A, Ding A, Du L, Jiang K, Ding Y, Sapisochin G, Ghanekar A.
and 200 ng was submitted for library preparation using TruSeq Stranded Total
RNA Library Prep
Gold (
20020598
;
Illumina
). Libraries were sequenced on an Illumina NextSeq500 System with 75‐bp length paired‐end mode
(77)
The cryptic gonadotropin-releasing hormone neuronal system of human basal ganglia
Elife
June 15, 2021
Katalin Skrapits, Miklós Sárvári, Imre Farkas, Balázs Göcz, Szabolcs Takács, Éva Rumpler, Viktória Váczi, Csaba Vastagh, Gergely Rácz, András Matolcsy,
et al.
Kit,
Agilent
,
5067-1513
, Commercial
assay
or kit,TruSeq Stranded Total
RNA Library Preparation
Gold Kit,
Illumina
,Cat#
20020598
, Commercial
assay
or kit,High
Sensitivity
DNA Kit,
Agilent
,
5067-4626
, Commercial
assay
or kit,NextSeq
(78)
Single-cell long noncoding RNA (lncRNA) transcriptome implicates MALAT1 in triple-negative breast cancer (TNBC) resistance to neoadjuvant chemotherapy
Cell Death Discovery
June 1, 2021
Hibah Shaath, Radhakrishnan Vishnubalaji, Ramesh Elango, Shahryar Khattak, Nehad M. Alajez
input for the library preparation by using TruSeq Stranded Total
RNA Library Prep
Gold kit (Cat #:
20020598
) from
Illumina
following the manufacturer’s protocol. In brief, 500 ng of total RNA was subjected to
(79)
Respiratory Co-Infections: Modulators of SARS-CoV-2 Patients’ Clinical Sub-Phenotype
Frontiers in Microbiology
May 28, 2021
Priyanka Mehta et al.
Whole transcriptome sequencing
was performed using the Illumina TrueSeq Stranded Total
RNA Library Prep
Gold (
Illumina
, Cat. No.
20020598
). A total RNA input of 500 ng of each sample was depleted of cytoplasmic and mitochondrial rRNA by Ribo-Zero
(80)
Transcription activation depends on the length of the RNA polymerase II C‐terminal domain
The EMBO Journal
May 3, 2021
Anna Sawicka, Gabriel Villamil, Michael Lidschreiber, Xavier Darzacq, Claire Dugast‐Darzacq, Björn Schwalb, Patrick Cramer
both, total and labeled
RNA‐seq
libraries) and TruSeq Stranded Total
RNA Library Prep
Gold (
Illumina
, #
20020598
), followed by
sequencing
with NextSeq500 (75 bases in paired‐end mode). PRO‐seq PRO‐seq was performed
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TruSeq Stranded Total RNA Library Prep Gold (48 Samples) from Illumina
Supplier Page
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