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Assay Kit
TruSeq Stranded Total RNA Library Prep Gold (48 Samples)
TruSeq Stranded Total RNA Library Prep Gold (48 Samples) from Illumina
Be the first to write a review!
Citations:
(102)
Supplier Page
Supplier Page from
Illumina for
TruSeq Stranded Total RNA Library Prep Gold (48 Samples)
Description
Whole-transcriptome stranded RNA-Seq with Ribo-Zero Gold rRNA depletion, also removes mitochondrial rRNA; 48-sample format
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
TruSeq Stranded Total RNA Library Prep Gold (48 Samples)
Company
Illumina
Price
Supplier Page
View Company Product Page
Catalog Number
20020598
Applications
Total RNA-Seq, Whole-transcriptome sequencing
Reactivity
Human, Mouse, Rat
Type
RNA Library Prep Kit
Quantity
48 Samples
Add to Compare List
Illumina
5200 Illumina Way
San Diego
,
CA
92122
United States
Phone
: 858.202.4500
Company Profile
Website:
http://illumina.com
Citations (102)
(21)
Cryopreservation of mesenchymal stem/stromal cells using a DMSO-free solution is comparable to DMSO-containing cryoprotectants: results of an international multicenter PACT/BEST collaborative study
Cytotherapy
December 1, 2024
Theodros Mamo, Cheryl A. Cox, Connor Demorest, Magali J. Fontaine, Allison Hubel, Linda Kelley, Aisha Khan, Denese C. Marks, Shibani Pati, Jo-Anna Reems,
et al.
Clara, CA, United States). cDNA libraries were generated using TruSeq Stranded Total RNA kit (Cat #
20020598
,
Illumina
, San Diego, CA, United States) according to its protocol and sequenced on
Illumina
NextSeq 550
(22)
PALB2-mutated human mammary cells display a broad spectrum of morphological and functional abnormalities induced by increased TGFβ signaling
Cellular and Molecular Life Sciences
December 1, 2024
Hanna Tuppurainen, Niina Laurila, Marjut Nätynki, Leila Eshraghi, Anna Tervasmäki, Louisa Erichsen, Claus Storgaard Sørensen, Katri Pylkäs, Robert Winqvist, Hellevi Peltoketo
Libraries were prepared from 100 ng of RNA using TruSeq® Stranded Total RNA LT Gold kit (
Illumina
#
20020598
). The quantity and quality of the libraries were assessed using Bioanalyzer 2100 with DNA 1000 Kit (
Agilent Technologies
(23)
Direct mitochondrial import of lactate supports resilient carbohydrate oxidation
bioRxiv
October 12, 2024
Ahmad A. Cluntun, Joseph R. Visker, Jesse N. Velasco-Silva, Marisa J. Lang, Luis Cedeño-Rosario, Thirupura S. Shankar, Rana Hamouche, Jing Ling, Ji Eon Kim, Ashish G. Toshniwal,
et al.
libraries were prepared as described using the
Illumina
TruSeq Stranded Total
RNA Library Prep
Gold kit (
20020598
) with TruSeq RNA UD Indexes (20022371). Purified libraries were qualified on an
Agilent Technologies
(24)
Enhancing mitochondrial pyruvate metabolism ameliorates ischemic reperfusion injury in the heart
JCI Insight
September 10, 2024
Joseph R. Visker, Ahmad A. Cluntun, Jesse N. Velasco-Silva, David R. Eberhardt, Luis Cedeño-Rosario, Thirupura S. Shankar, Rana Hamouche, Jing Ling, Hyoin Kwak, J. Yanni Hillas,
et al.
libraries were prepared as described using the
Illumina
TruSeq stranded Total
RNA Library Prep
Gold kit (
20020598
) with TruSeq RNA UD Indexes (20022371). Purified libraries were qualified on an
Agilent Technologies
(25)
A simple and scalable zebrafish model of Sonic hedgehog medulloblastoma
Cell Reports
August 27, 2024
Mattie J. Casey, Priya P. Chan, Qing Li, Ju-Fen Zu, Cicely A. Jette, Missia Kohler, Benjamin R. Myers, Rodney A. Stewart
5067–5579
and
5067–5580
). The Illumina TruSeq Stranded Total RNA kit with Ribo-Zero Gold (
Illumina
20020598
) was used for library preparation.
Sequencing
libraries were chemically denatured and applied to Illumina
(26)
Amino acids trigger MDC-dependent mitochondrial remodeling by altering mitochondrial function
bioRxiv
July 13, 2024
Nidhi Raghuram, Adam L. Hughes
RNA sequencing
libraries were prepared using
Illumina
TruSeq Stranded Total
RNA Library Prep
Gold kit (
20020598
) with TruSeq RNA UD Indexes (20022371). Purified libraries were qualified on an
Agilent Technologies
(27)
Discovery and functional characterization of LncRNAs associated with inflammation and macrophage activation
Methods
July 1, 2024
Avisankar Chini, Prarthana Guha, Ashcharya Rishi, Monira Obaid, SM Nashir Udden, Subhrangsu S. Mandal
were prepared (as described) using the Illumina TruSeq Stranded Total
RNA Library Prep
Gold kit (Cat#
20020598
,
Illumina
, USA) with IDT (Integrated DNA Technologies, Inc.) -for Illumina TruSeq RNA UD Indexes (Cat#
(28)
Multiomic profiling of transcription factor binding and function in human brain
Nature Neuroscience
July 1, 2024
Jacob M. Loupe, Ashlyn G. Anderson, Lindsay F. Rizzardi, Ivan Rodriguez-Nunez, Belle Moyers, Katie Trausch-Lowther, Rashmi Jain, William E. Bunney, Blynn G. Bunney, Preston Cartagena,
et al.
respectively) was used as input for TruSeq stranded Total
RNA Library Prep
Gold kit (
Illumina
, cat. no.
20020598
) according to manufacturer’s protocol with TruSeq RNA UD Indexes (Illumina, cat. no. 20022371).
Sequencing
(29)
Transcriptomic convergence despite genomic divergence drive field cancerization in synchronous squamous tumors
Frontiers in Oncology
June 13, 2024
Qiu Xuan Tan, Nicholas B. Shannon, Weng Khong Lim, Jing Xian Teo, Daniel R. Y. Yap, Sze Min Lek, Joey W. S. Tan, Shih Jia J. Tan, Josephine Hendrikson, Ying Liu,
et al.
generated using the Truseq Stranded Total
RNA Library Prep
Gold kit (
Illumina
, San Diego, CA, USA; cat. #
20020598
) and subsequently sequenced on the HiSeq2000 system (Illumina) using the paired-end 76 bases run setting. 3.3
(30)
A Novel Strain of Fusarium oxysporum Alternavirus 1 Isolated from Fusarium oxysporum f. sp. melonis Strain T-BJ17 Confers Hypovirulence and Increases the Sensitivity of Its Host Fungus to Difenoconazole and Pydiflumetofen
Viruses
June 2, 2024
Huihui Hua, Xinyi Zhang, Li Liu, Xuehong Wu
rRNA-depleted total RNA by using TruSeq Stranded Total
RNA Library Prep
Gold (
Illumina
, San Diego, CA, USA,
20020598
). The library quality was checked using the
Qubit® 2.0 Fluorometer
(
Invitrogen
, Carlsbad, CA, USA,
Q32866
)
(31)
Comparative transcriptomic analysis reveals differences in gene expression and regulatory pathways between nonacral and acral melanoma in Asian individuals
The Journal of Dermatology
May 1, 2024
Yu‐Jen Chiu, Cheng‐Yuan Li, Tien‐Hsiang Wang, Hsu Ma, Teh‐Ying Chou
subsequent steps were performed using a TruSeq Stranded Total
RNA Library Prep
Gold Kit (category number
20020598
,
Illumina
) in accordance with the respective manuals. 21 , 22 , 23 2.3
Whole‐transcriptome sequencing
Total
(32)
A new genomic framework to categorize pediatric acute myeloid leukemia
Nature genetics
February 1, 2024
Umeda M, Ma J, Westover T, Ni Y, Song G, Maciaszek JL, Rusch M, Rahbarinia D, Foy S, Huang BJ,
et al
.
were constructed using the TruSeq Stranded Total RNA Kit, with Ribozero Gold (
Illumina
, catalog no.
20020598
) for
RNA-seq
, the TruSeq DNA
PCR
-Free
Library Prep
Kit (
Illumina
, catalog no.
20015963
) for
WGS
and
(33)
ABCB1 overexpression through locus amplification represents an actionable target to combat paclitaxel resistance in pancreatic cancer cells
Journal of Experimental & Clinical Cancer Research
January 2, 2024
Cecilia Bergonzini, Alessandro Gregori, Tessa M. S. Hagens, Vera E. van der Noord, Bob van de Water, Annelien J. M. Zweemer, Bircan Coban, Mjriam Capula, Giulia Mantini, Asia Botto,
et al.
Library preparation was performed using the Illumina TruSeq Stranded total
RNA Library Prep
gold Kit (
20020598
,
Illumina Inc
., San Diego, USA) and Agencount AMPure XP beads (Beckman Coulter, Brea, USA). Library
(34)
Spatially coordinated heterochromatinization of long synaptic genes in fragile X syndrome
Cell
December 21, 2023
Thomas Malachowski, Keerthivasan Raanin Chandradoss, Ravi Boya, Linda Zhou, Ashley L. Cook, Chuanbin Su, Kenneth Pham, Spencer A. Haws, Ji Hun Kim, Han-Seul Ryu,
et al.
for
RNA-seq
library preparation using the TruSeq Stranded Total
RNA Library Prep
Gold kit (
Illumina
,
20020598
) according to the manufacturer’s instructions. Briefly, we removed rRNA from the input RNA, generated
(35)
Dual RNA-Seq reveals transcriptionally active microbes (TAMs) dynamics in the serum of dengue patients associated with disease severity
Frontiers in Microbiology
November 30, 2023
Aanchal Yadav, Pallawi Kumari, Priti Devi, Jorelle Jeanne B. Adjele, Sandeep Budhiraja, Bansidhar Tarai, Rajesh Pandey
library preparation using the Illumina TruSeq Stranded Total
RNA Library Prep
Gold (
Illumina
, Cat. No.
20020598
). The final library was quantified using a Qubit double-stranded DNA (dsDNA) high-
sensitivity
(HS)
assay
(36)
Therapeutic targeting of the TPX2/TTK network in colorectal cancer
Cell Communication and Signaling
September 28, 2023
Hibah Shaath, Radhakrishnan Vishnubalaji, Ramesh Elango, Dinesh Velayutham, Puthen Veettil Jithesh, Nehad M. Alajez
as input for the library preparation using TruSeq Stranded Total
RNA Library Prep
Gold kit (Cat #:
20020598
) from
Illumina
following the manufacturer’s protocol. In brief, 100 ng of total RNA was subjected to
(37)
Enhanced Angiogenesis in HUVECs Preconditioned with Media from Adipocytes Differentiated from Lipedema Adipose Stem Cells In Vitro
International journal of molecular sciences
September 1, 2023
Al-Ghadban S, Walczak SG, Isern SU, Martin EC, Herbst KL, Bunnell BA.
RNA Sequencing
RNAseq libraries were prepared using TruSeq Stranded Total
RNA Library Prep
kits (
Illumina
, Cat #:
20020598
) following the manufacturer’s instructions. Briefly, one microgram of total RNA was used as starting
(38)
Caveolin-1 promotes glioma progression and maintains its mitochondrial inhibition resistance
Discover Oncology
August 29, 2023
Yu’e Liu, Yi Chen, Fei Wang, Jianghua Lin, Xiao Tan, Chao Chen, Lei-lei Wu, Xiaoling Zhang, Yi Wang, Yufeng Shi,
et al.
the criteria for library preparation. TruSeq Stranded Total RNA with Ribo-Zero Gold kit (
Illumina
,
20020598
) was used to prepare the sample;
PCR
was used to generate the library. Agilent Bioanalyzer 2200, 150
(39)
Early transcriptomic host response signatures in the serum of dengue patients provides insights into clinical pathogenesis and disease severity
Scientific Reports
August 29, 2023
Aanchal Yadav, Uzma Shamim, Varsha Ravi, Priti Devi, Pallawi Kumari, Ranjeet Maurya, Poonam Das, Madhuri Somani, Sandeep Budhiraja, Bansidhar Tarai,
et al.
library preparation using Illumina TruSeq Stranded Total
RNA Library Prep
Gold (
Illumina
, Cat. No.
20020598
) as per manufacturer’s reference guide (1000000040499 v00). The ribosomal RNA (both cytoplasmic and
(40)
Hedgehog target genes regulate lipid metabolism to drive basal cell carcinoma and medulloblastoma
Research square
August 3, 2023
Daggubati V, Vykunta A, Choudhury A, Qadeer Z, Mirchia K, Saulnier O, Zakimi N, Hines K, Paul M, Wang L,
et al
.
Qiagen
). Library preparation was performed using the TruSeq Stranded Total
RNA Library Prep
Gold Kit (
20020598
,
Illumina
) and 150 bp paired-end reads were sequenced on an Illumina NovaSeq 6000 to at least 88 million
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TruSeq Stranded Total RNA Library Prep Gold (48 Samples) from Illumina
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