Cell Counting Kit 8 (WST-8 / CCK8) ab228554 from Abcam

Product Specs
ItemCell Counting Kit 8 (WST-8 / CCK8)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab228554
Quantity1000 Test, 5000 Tests
Sample TypeAdherent cells, Suspension cells

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Website: http://www.abcam.com

(1) hiPSC-Derived M1 Macrophages Exhibit Synergistic Therapeutic Effects with Paclitaxel in Ovarian CancerInternational Journal of Stem CellsFebruary 28, 2028Suji Jeong, Seho Cha, Haengseok Song, Heesoon Chang, Sung Hun Na, Seok Ho Hong, Mira Parkconditions. CCK8 assay Cell viability was assessed using Cell Counting Kit-8 (WST-8/CCK8) (Abcam; #ab228554) according to the manufacturer’s instructions. A ready-to-use CCK8 solution was added to the cell culture medium

(2) HSPA8 orchestrates SNARE complex assembly to drive extracellular vesicle-mediated spread of p-tau217 in Alzheimer's diseaseTransl NeurodegenerAugust 27, 2026Bin Xu, Zhen Guo, Jun Chen, Yazhou Xie, Pan Wang, Xiao Xiong, Jiayi Yu, Zhi Xu, Yuan Fu, Zhuoqing Lan, et al.were recorded. Cell viability assay Cell viability was assessed using a CCK-8 assay kit (Abcam, Cat# ab228554, Cambridge, UK) in accordance with the manufacturer’s instructions. Cells were plated in 96-well plates,

(3) N-Myristoyltransferase 2 Is Regulated by Polyphosphate-Driven Lysine InteractionsBiomoleculesJuly 27, 2026Isabella Martins, Kaia Bailey, Anni Ge, Ethan Belrose, Xiaolong Yang, Zongchao JiaMetabolic activity was assessed by adding 10 µL of metabolic activity reagent (Abcam, Waltham, MA, USA, Cat#AB228554) per 100 µL of culture medium, followed by incubation for 4 h at 37 °C. Absorbance was subsequently

(4) The Cuproptosis-Associated AL139023.1/miR-139-5p/ELOVL5 ceRNA Axis Regulates Proliferation, Migration, and Cell Cycle in Esophageal Squamous Cell CarcinomaCancer Manag ResJuly 18, 2026Kai Liang, Yun Gao, Xiangyu Zhang, Jin Yang103/well in 96-well plates, incubated overnight (37°C, 5% CO2), and treated with 10 µL CCK-8 reagent (ab228554, Abcam, USA) for 3 h. Absorbance at 450 nm was measured on a Molecular Devices microplate reader and

(5) Spray-Pyrolyzed Hollow and Yolk–Shell CeO 2 Nanocarriers with Tunable Structure for Redox-Responsive Delivery and Gene Rescue ApplicationsACS Applied Materials & InterfacesJuly 15, 2026Jalal Poostforooshan, Cléa Chesneau, Laurent Michely, Madara Dias Wickramanayaka, Iwona Pranke, Patrick Balaguer, Abdelhay Boulahtouf, Fabrice Lejeune, Benedikt Eberhardt, Alexandre Hinzpeter, et al.photocolorimetric analysis using the Cell Counting Kit 8 (WST-8) according to the manufacturer’s guidelines (Abcam, Ab228554). This involved diluting CCK8 in DMEM with FBS (1:10 ratio) and adding 100 μL to each well. The cells

(6) Kaempferol Attenuates Spaceflight-Associated Knee Cartilage Degradation by Targeting NOX4-Mediated Mitochondrial DysfunctionAdv Sci (Weinh)July 9, 2026Yuesong Yin, Ruiling Xu, Meagan J. Makarczyk, Jia-Jun Liu, Silvia Liu, Matthew Z. Shi, Roberta Di Carlo, Michael F. Almeida, Alex Garris, Sarah Day, et al.graded Kaempferol concentrations, and viability was measured at days 0, 1, 3, and 7 using CCK8 (Abcam, ab228554). 5.15 SiRNA Transfection Human primary chondrocytes (∼70% confluence) were transfected with siRNA

(7) Integrative serum metabolite prioritization and functional screening identify N-acetyl-L-glutamine as a protective candidate in premature ovarian insufficiencyFront GenetJuly 2, 2026Xinyue Zhang, Chen Chen, Xiaolan Zhuwashed with phosphate-buffered saline (PBS). Fresh medium containing 10% Cell Counting Kit-8 reagent (Ab228554, Abcam) was then added to each well. After incubation for 2 h at 37 °C in the dark, absorbance at 450

(8) Arsenic exposure promotes retinal pigment epithelium dysfunction and oxidative stress via NF-κB pathwayScientific ReportsJune 8, 2026Lan Zhong, Yumei Tao, Liming Taoand cytotoxicity assessment Cell viability was assessed using a Cell Counting Kit-8 (CCK-8, Abcam, ab228554). ARPE-19 cells were seeded into 96-well plates and treated as described. Following treatment, CCK-8

(9) Hyperoside protects against poly-GR-mediated neurodegeneration via regulation of mitochondrial fission and oxidative stress in C9orf72-associated ALSChin MedJune 4, 2026Wen-Chi Hsieh, Chun-Yu Lin, Hsuan-Cheng Wu, Eddie Feng-Ju Weng, Shao-Ming Wangincubator for the indicated periods. Cell viability was assessed using the Cell Counting Kit-8 (CCK-8; ab228554, Abcam). Diluted CCK-8 reagent was added to each well and incubated for 1 h at 37 °C, after which absorbance

(10) Exposure to high doses of tyre antioxidant 6PPD causes senescence to induce unexplained miscarriage by suppressing BAZ1B-mediated ubiquitination degradation of P21EBioMedicineJune 1, 2026Waiping Zhang, Yan Liang, Yi Sun, Junchang Wang, Wenxin Huang, Shuaishuai Xing, Zhihong Zhang, Yanbing Lin, Liqin Zeng, Qiong Lei, et al.Western blotting. Cell viability Cell viability was evaluated using a Cell Counting Kit-8 (CCK8, ab228554, Abcam, Cambridge, UK). Cells (5 × 103 cells per well, three replicates per group) were seeded in 96-well

(11) Epigenetic brain reprogramming rejuvenates neuro-immune circuits to reverse Alzheimer’s disease pathology and systemic bone lossJournal of NeuroinflammationMay 28, 2026Mei-Dan Wan, Xi-Xi Liu, Teng-Fei Wan, Yi-Wei Liu, Ya-Ling Jiang, Jing-Tao Zou, Bin Jiao, Qian-Qian Liu, Ling Jin, Ran Duan, et al.without AAV-OSK pretreatment. Cell proliferation was assessed after 48 h using the CCK-8 assay (Abcam, ab228554). Flow cytometry Apoptosis in HT22 cells was assessed by flow cytometry following CCCP treatment.

(12) A MUC1-ALIX complex regulates extracellular vesicle cargo loading with activated SRC to enhance pancreatic cancer progressionCancer LettersMay 28, 2026Ying Huang, Kristine V. Hoagstrom, Clara S. Mundry, Tuo Hu, Heather C. Jensen-Smith, Jacob Wragge, Phong Le, Amina Baniya, Kyle L. McAndrews, Thomas C. Caffrey, et al.Lipofectamine 3000,ThermoFisher,Cat#: L3000015 APC/Cy7 Conjugation Kit,Abcam,Cat#: ab102859 Cell Counting Kit 8,Abcam,Cat#: ab228554 CellTiter-Glo® Luminescent Cell Viability Assay,Promega,Cat#: G7572 Cy5™ Mono-Reactive Dye,Amersham,Cat#:

(13) Polyphosphate as a novel regulator of super-enhancer complexes: disruption of phase separation and gene expressionNucleic Acids ResMay 26, 2026Zhiyun Yang, Zongchao Jiaobjective or Leica Mica Microhub confocal microscope. WST-8 assay WST-8 kit was purchased from Abcam (#ab228554), and assay was conducted according to the manufacturer’s instructions. Briefly, 1.5 × 104 cells/well

(14) Endothelial Drp1 integrates VEGF-induced redox signaling with glycolysis through cysteine oxidation to drive angiogenesisNature CommunicationsMay 20, 2026Sheela Nagarkoti, Young-Mee Kim, Archita Das, Dipankar Ash, Eric A. Vitriol, Tracy-Ann Read, Varadarajan Sudhahar, Md. Selim Hossain, Shikha Yadav, Stephanie Kelley Spears, et al.Cell viability was measured using CCK8 assay. For CCK8 assay, Cell Counting Kit 8 (WST-8/CCK8) (Abcam, ab228554) was used. CCK-8 solution (CCK8: medium, 1:10) was added into each dish and incubated in the dark at

(15) Elevated GCN2 levels in cancer cells confer protection from mitotic stress and faster cell movementCell Oncol (Dordr)May 11, 2026Vilte Stonyte, Christina Saeten Fjeldbo, Mélanie Langiu, Michelle Joy-Immediato, Priyanka Swaminathan, Prabin Sharma Humagain, Laura Marian Valencia-Pesqueira, Lilian Lindbergsengen, Coen Campsteijn, Heidi Lyng, et al.determined either by crystal violet staining and counting, or by the CCK cell proliferation assay (Abcam, #ab228554). Chemotaxis assays were performed using µ-Slide Chemotaxis slides (Ibidi # 80326), live-cell imaging

(16) Electronics-free soft robotic minitablet for on-demand gastric molecular sensing and diagnostics in vivoSci AdvMay 8, 2026Chen Wang, Rui Shi, Anatolii Abalymov, Hao Bao, Thomas Ka-Yam Lam, Zihan Wang, Yongfeng Mei, Zongwei Cai, Xiang-Zhong Chen, Sarthak Misra, et al.removed and replaced with 100 μl of culture medium containing 10% CCK-8 reagent (Abcam, catalog no. ab228554) followed by 1 hour of incubation. Absorbance was measured at 450/650 nm using an Infinite F200 PRO

(17) 3D spheroids of umbilical cord-derived MSCs protect retinal pigment epithelium against oxidative and inflammatory injury by activating autophagyStem Cell Research & TherapyMay 2, 2026Qian Xu, Mengyao Li, Dong Li, Xiaoyu Dai, Yifei Zhang, Yi Quviability assessment ARPE-19 cell viability was assessed using the Cell Counting Kit-8 (CCK-8, Abcam #ab228554) colorimetric assay. ARPE-19 cells were plated in 96-well plates at 1 × 10^4 cells per well and incubated

(18) METTL14 modulates the autophagy-pyroptosis pathway in fibroblasts by modifying BECN1 through m6A methylation to promote wound healing in DFUs and SYD treatmentCell Biology and ToxicologyMay 1, 2026Zhaowei Wang, Zongqiang Wei, Qinyao Yu, Linru Wang, Kai Liu, Hongjie Wang, Qiang Liplasmid, followed by exposure to HG conditions for 48 h. Subsequently, CCK-8 reagent (10 μL per well, ab228554, Abcam) was applied, and cells were incubated for 2 h at 37 ℃. Absorbance at 450 nm was subsequently

(19) Novel N-chloroacetyl-2-pyrazoline analogs with 2-naphthyl and ferrocenyl groups: targeting histamine receptor H1 to overcome colorectal cancer drug resistanceCancer Drug ResistanceApril 30, 2026Dileepkumar Veeragoni, Hindole Ghosh, Affan Ansari, Sangita Bhattacharyya, Ahraar Azaz, Ojaswitha Ommi, Obdulia Covarrubias-Zambrano, Leonhard H. F. Köhler, Justus F. Ködel, Ameer Hamza, et al.subtracted from the formazan absorbance readings. A cell counting kit-8 (CCK-8) assay (Abcam, Cat no. ab228554, Burlingame, CA, USA) was used to assess cell viability in THP-1 and Jurkat cells. THP-1 and Jurkat cells

(20) Poly-GR promotes ferroptosis-associated vulnerability in C9orf72-ALSCell & BioscienceApril 26, 2026Chun-Yu Lin, Wen-Chi Hsieh, Shao‐Ming Wangfor the indicated durations. Cell viability was then quantified using the Cell Counting Kit-8 (CCK-8; ab228554, Abcam). The diluted CCK-8 reagent was added to each well and incubated for 1 h at 37 °C, after which

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(1)

4/19/2023Great Kit for Cell Viability and More Seth TompkinsThis kit is marketed as a way to measure cell viability. This is true, but it can do so much more. The assay works by measuring active mitochondria (more mitochondria, the more the dye is reduced). So... Read Review
(1)

10/4/2022Good Cell Viability Assay Kit Yashavantha VishweshwaraiahWe used this kit for cell viability microplate assay using various cell lines and expression conditions. We got consistent and reproducible results.

Cell Counting Kit 8 (WST-8 / CCK8) from Abcam

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Cell Counting Kit 8 (WST-8 / CCK8) from Abcam

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