DAPI Staining Solution ab228549 from Abcam

Product Specs
ItemDAPI Staining Solution
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab228549
Quantity2 mL
Sample TypeAdherent cells, Suspension cells
TypeStain

Add to Compare List

Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(281) Nonrandom DNA Segregation Detection under Replication StressSTAR ProtocolsDecember 30, 2020Sisi Chen, Fang Ji, Meichun Xing, Gaoying Chen, Jiaqian Li, Xinwei Geng, Chao Zhang, Madiha Zahra Syeda, Songmin Yingfrom Sigma (T8787); BSA can also be obtained from Sigma (A1933); DAPI can also be obtained from Abcam (ab228549). Step-By-Step Method Details CldU Administration Timing: 2 weeks Cells are treated with CldU (1

(282) Oxidative stress mediates thalidomide-induced pain by targeting peripheral TRPA1 and central TRPV4BMC biologyDecember 14, 2020De Logu F, Trevisan G, Marone IM, Coppi E, Padilha Dalenogare D, Titiz M, Marini M, Landini L, Souza Monteiro de Araujo D, Li Puma S, et al.at RT. Slides were then coverslipped with mounting medium with 4′,6-diamidino-2-phenylindole (DAPI, #ab228549, Abcam). Lumbar (L4–L6) spinal cord collected from perfused mice was placed overnight at 4 °C in 10%

(283) Fruquintinib inhibits VEGF/VEGFR2 axis of choroidal endothelial cells and M1-type macrophages to protect against mouse laser-induced choroidal neovascularizationCell death & diseaseNovember 27, 2020Liu X, Guo A, Tu Y, Li W, Li L, Liu W, Ju Y, Zhou Y, Sang A, Zhu M.anti-F4/80 antibody (#ab105156, Abcam, USA) antibodies, and 4′, 6-diamidino-2-phenylindole (DAPI; #ab228549, Abcam) were used in the immunofluorescence. Hematoxylin–eosin stain On day 7, following euthanasia,

(284) Noise Exposures Causing Hearing Loss Generate Proteotoxic Stress and Activate the Proteostasis NetworkCell reportsNovember 24, 2020Jongkamonwiwat N, Ramirez MA, Edassery S, Wong ACY, Yu J, Abbott T, Pak K, Ryan AF, Savas JN.were then rinsed three times in PBS for 15 minutes and stained with DAPI Staining Solution (Abcam, ab228549) for 5 minutes prior to mounting in Fluoromount-G (Thermo Scientific, cat. 00–4958-02). Tissues were

(285) Laquinimod Inhibits Inflammation-Induced Angiogenesis in the CorneaFrontiers in MedicineNovember 10, 2020Zuohong Li, Jianping Chen, Lei Lei, Nan Jiang, Yanling Zhu, Yu Jia, Yehong Zhuo, Wenru SuAlexa Fluor 555 (Cell Signaling Technology, Germany, 1:1000). After counterstaining with DAPI (Abcam, ab228549), the sections were mounted with antifade mounting medium (Beyotime, Shanghai, China). The sections

(286) Enterococcus faecalis Induces Differentiation of Immune-Aberrant Dendritic Cells from Murine Bone Marrow-Derived Stem CellsInfection and ImmunityOctober 19, 2020Mohamed Mohamed Elashiry, Mahmoud Elashiry, Rana Zeitoun, Ranya Elsayed, Fucong Tian, Shehab Eldin Saber, Salma Hasan Elashry, Franklin R. Tay, Christopher W. Cutler(Thermo Fisher Scientiﬁc), and the nuclei were counterstained with 4=,6-diamidino-2-phenylindole (DAPI) (ab228549; Abcam, Burlingame, CA) and visualized using an inverted confocal microscope (LSM 780; Carl Zeiss AG, Oberkochen,

(287) P2X7 Receptor (P2X7R) of Microglia Mediates Neuroinflammation by Regulating (NOD)-Like Receptor Protein 3 (NLRP3) Inflammasome-Dependent Inflammation After Spinal Cord InjuryMedical science monitor : international medical journal of experimental and clinical researchSeptember 21, 2020Fan X, Ma W, Zhang Y, Zhang L.Thermo Fisher Scientific, Shanghai, China) at room temperature for 2 h in the dark and then incubated with DAPI (1: 1000, ab228549, Abcam, Shanghai, China) at room temperature for 3 min. Sections were observed under a confocal laser

(288) Silencing glioma‑associated oncogene homolog 1 suppresses the migration and invasion of hepatocellular carcinoma in vitroOncology LettersSeptember 11, 2020Zeming Hu, Fangfang Xie, Ang Hu, Mengjing Xu, Yuwen Liu, Jiankang Zhang, Jianbo Xiao, Yunlei Song, Jianing Zhong, Bin ChenAfter washing three times with PBST, the cell nuclei were stained with DAPI (1 µg/ml, Abcam, cat. no. ab228549) for 10 min at room temperature in the dark and detected with a confocal laser scanning microscope (LSM

(289) Functional recovery with histomorphometric analysis of nerves and muscles after combination treatment with erythropoietin and dexamethasone in acute peripheral nerve injuryPLOS ONESeptember 3, 2020Jung Il Lee, Jeong Min Hur, Jooyoung You, Duk Hee Leeperipheral nerve injury 1:200, ab31851, Abcam); blue (40 ,6-diamidino-2-phenylindole [DAPI] 1:1000, ab228549, Abcam); control, staining without primary antibody; uninjured, normal nerve; saline, nerve treated

(290) TRPA1 mediates damage of the retina induced by ischemia and reperfusion in miceCell death & diseaseAugust 15, 2020Souza Monteiro de Araújo D, De Logu F, Adembri C, Rizzo S, Janal MN, Landini L, Magi A, Mattei G, Cini N, Pandolfo P, et al.Sections were coverslipped using a water-based mounting medium with 4′6′-diamidino-2-phenylindole (DAPI, #ab228549, Abcam, Cambridge, UK). The analysis of negative controls (nonimmune serum) was simultaneously performed

(291) Microdomains form on the luminal face of neuronal extracellular vesicle membranesScientific reportsJuly 20, 2020Matthies D, Lee NYJ, Gatera I, Pasolli HA, Zhao X, Liu H, Walpita D, Liu Z, Yu Z, Ioannou MS.RRID:AB_2313773) and goat anti-mouse alexafluor488 (Invitrogen, A11001) and stained with DAPI (Abcam, ab228549). Neurons were routinely monitored for viability and mycoplasma contamination by imaging nuclei stained

(292) Caspase1/11 signaling affects muscle regeneration and recovery following ischemia, and can be modulated by chloroquineMolecular medicine (Cambridge, Mass.)July 8, 2020Sachdev U, Ferrari R, Cui X, Pius A, Sahu A, Reynolds M, Liao H, Sun P, Shinde S, Ambrosio F, et al.microtubule associated protein 1A/1B-light chain 3 (LC3; #L8918, Sigma,Sigma-Aldrich, St. Louis, MO) DAPI (#ab228549; Abcam), Cy-3-conjugated secondary antibodies (#ab6939;Abcam); antibody to myosin heavy chain fast (#ab51263)

(293) Mechanically Robust, Softening Shape Memory Polymer Probes for Intracortical RecordingMicromachines (Basel)June 25, 2020Allison M. Stiller, Joshua O. Usoro, Jennifer Lawson, Betsiti Araya, María Alejandra González-González, Vindhya R. Danda, Walter E. Voit, Bryan J. Black, Joseph J. Pancrazioanti-mouse Alexa Fluor 555 (1:200, Abcam, ab150118), along with 0.06% concentration(0.06%, DAPI Abcam,Abcam, ab228549). Sections were then 3 times for 10for min PBS The The PBS PBS was (0.06%, ab228549). Sections werewashed then

(294) Effects and mechanism of Lanthanum Citrate on the proliferation and apoptosis of hepatocellular carcinoma cell line SMMC-7721The Turkish Journal of GastroenterologyMay 11, 2020Bin Chen, Department of Hepatobiliary Surgery, the First Affiliated Hospital of Gannan Medical University, Ganzhou, China, Zeming Hu, Biao Chen, Bofei Li, Gannan Medical University, Ganzhou, China, Gannan Medical University, Ganzhou, China, Department of General Surgery, Rucheng County People’s Hospital, Chenzhou, China1 h. The cells were washed three more times with PBST and then incubated with DAPI (1µg/ ml, Abcam, ab228549, USA) and detected with a Confocal Laser Scanning Microscope (Carl Zeiss LSM 880). Statistical analysis SPSS

(295) miR‑217‑5p regulates myogenesis in skeletal muscle stem cells by targeting FGFR2Molecular Medicine ReportsMay 7, 2020Menghai Zhu, Gang Chen, Yi Yang, Jiantao Yang, Bengang Qin, Liqiang Guwith 2% goat serum (Gibco; Thermo Fisher Scientific, Inc.) for 1 h at room temperature. DAPI (cat. no. ab228549, Abcam) was used to identify the nuclei. The cells were incubated with primary antibodies overnight

(296) ZEB2-AS1 Accelerates Epithelial/Mesenchymal Transition Through miR-1205/CRKL Pathway in Colorectal CancerCancer Biotherapy and RadiopharmaceuticalsMarch 1, 2020Yinghao Jiang, Guangming Liu, Wei Ye, Jianjin Xie, Chunfa Shao, Xiaowei Wang, Xia LiIgG (Vector Labs), was added. The cells were counterstained with 4¢,6¢-diamidino-2phenylindole (DAPI; ab228549, Abcam, Cambridge, United Kingdom). All the above procedures were carried out in a dark room. The samples

(297) rTMS Regulates the Balance Between Proliferation and Apoptosis of Spinal Cord Derived Neural Stem/Progenitor CellsFrontiers in Cellular NeuroscienceJanuary 28, 2020Chen-Guang Zhao, Jie Qin, Wei Sun, Fen Ju, Yong-Lin Zhao, Rui Wang, Xiao-Long Sun, Xiang Mou, Hua YuanA-11035, Thermo Fisher Scientific, Waltham, MA, USA) and 4’,6-diamidino-2-phenylindole (DAPI; 1:1,000, ab228549, Abcam, Cambridge, UK) for 45 min at room temperature. Finally, samples were washed with phosphate buffered

(298) GCNA Interacts with Spartan and Topoisomerase II to Regulate Genome StabilityDevelopmental cellJanuary 6, 2020Dokshin GA, Davis GM, Sawle AD, Eldridge MD, Nicholls PK, Gourley TE, Romer KA, Molesworth LW, Tatnell HR, Ozturk AR, et al.dilutions at room temperature for 2 hours, washed twice in PBST, and secondary antibodies and DAPI (Abcam ab228549) were applied at 1:1000 dilution for 1 hours in darkness prior to mounting and imaging. Live cell imaging

(299) Evidence of SARS-CoV2 Entry Protein ACE2 in the Human Nose and Olfactory BulbCells, tissues, organsJanuary 1, 2020Klingenstein M, Klingenstein S, Neckel PH, Mack AF, Wagner AP, Kleger A, Liebau S, Milazzo A.After several washing steps, secondary antibodies were diluted 1:100 in PBS together with DAPI (Abcam, ab228549) and incubated for 45 min in room temperature away from daylight. The following secondary antibodies

(300) Fuzheng Huayu Recipe Prevented and Treated CCl4-Induced Mice Liver Fibrosis through Regulating Polarization and Chemotaxis of Intrahepatic Macrophages via CCL2 and CX3CL1Evidence-based complementary and alternative medicine : eCAMJanuary 1, 2020Zhang M, Liu HL, Huang K, Peng Y, Tao YY, Zhao CQ, Hu XD, Liu CH.donkey anti-rat IgG H&L (Alexa Fluor 647) (cat no: ab150155) were purchased from Abcam, UK. DAPI (cat no: ab228549) was purchased from Abcam, UK. Flow cytometric antibodies including anti-CD45 (cat no: 557659), anti-CD11b

Write a Review

(1)

6/17/2020This DAPI Binds Strongly to Nuclei Samantha LanjewarIt stains nuclei well and is easy to use.

DAPI Staining Solution from Abcam

Abcam

Citations (319)

Reviews

DAPI Staining Solution from Abcam

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map