DAPI Staining Solution ab228549 from Abcam

Product Specs
ItemDAPI Staining Solution
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab228549
Quantity2 mL
Sample TypeAdherent cells, Suspension cells
TypeStain

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(241) Antitumor Effects of Ursolic Acid through Mediating the Inhibition of STAT3/PD-L1 Signaling in Non-Small Cell Lung Cancer CellsBiomedicinesMarch 13, 2021Kang DY, Sp N, Lee JM, Jang KJ.Morphological Analysis Chromatin condensation during apoptosis was examined with DAPI staining solution (ab228549, Abcam, Cambridge, MA, USA). A549 and H460 cells were seeded in 6-well plates at a density of 1.5 ×

(242) Surfactant location and internal phase volume fraction dictate emulsion electrospun fiber morphology and modulate drug release and cell responseBiomaterials ScienceFebruary 23, 2021Pamela M. Johnson, Kelsey E. Knewtson, Jacob G. Hodge, Justin M. Lehtinen, Anna S. Trofimoff, D. Joseph Fritz, Jennifer L. Robinsonwith 300 µL PBS. Nuclei were stained for 15 minutes at room temperature RT using DAPI (100 µM; Abcam, ab228549). Meshes were washed 2x with 300 µL of PBS before being imaged on a Zeiss Axio Observer A1 Inverted

(243) Astragaloside IV inhibits palmitic acid-induced apoptosis through regulation of calcium homeostasis in mice podocytesMolecular Biology ReportsFebruary 19, 2021Yingjun Zang, Shuang Liu, Aili Cao, Xiangyu Shan, Wenjuan Deng, Zhijun Li, Hao Wang, Yunman Wang, Li Wang, Wen PengMolecular Probes). The mitochondria were labeled with Mito tracker and the nuclei were visualized by DAPI (ab228549, Abcam, Cambridge, MA, USA) staining. Images were taken using LEICA laser scan microscope. Apoptosis assay

(244) Neonatal AAV gene therapy rescues hearing in a mouse model of SYNE4 deafnessEMBO Molecular MedicineFebruary 5, 2021Shahar Taiber, Roie Cohen, Ofer Yizhar‐Barnea, David Sprinzak, Jeffrey R Holt, Karen B Avrahammyosin VIIa (Proteus Biosciences 25‐6790) 1:250, mouse anti‐FLAG (Sigma F3165) 1:1,000, DAPI (Abcam ab228549) 1:1,000, goat anti‐mouse (Abcam ab150119) 1:250, and goat anti‐rabbit Alexa Fluor 488 (Cell Signaling

(245) Circ_0030235 knockdown protects H9c2 cells against OGD/R-induced injury via regulation of miR-526bPeerJJanuary 1, 2021Zhang Y, Liu S, Ding L, Wang D, Li Q, Li D.the dark for 30 min at room temperature. Cell nucleus was stained with DAPI staining solution (10 mM, ab228549; Abcam) for 5 min. For quantification of apoptosis, the cells were observed by a confocal microscope

(246) TFF-1 Functions to Suppress Multiple Phenotypes Associated with Lung Cancer ProgressionOncoTargets and therapyJanuary 1, 2021Minegishi K, Dobashi Y, Tsubochi H, Hagiwara K, Ishibashi Y, Nomura S, Nakamura R, Ohmoto Y, Endo S.Cambridge, UK) IgG (1:1000) for 1h at RT. Samples were stained with 4′,6-diamidino-2-phenylindole (DAPI, ab228549, Abcam) and the fluorescence images were digitally captured on an All-in-One fluorescence microscope

(247) NLRP3 Inflammasome Activation of Mast Cells by Estrogen via the Nuclear-Initiated Signaling Pathway Contributes to the Development of EndometriosisFrontiers in immunologyJanuary 1, 2021Guo X, Xu X, Li T, Yu Q, Wang J, Chen Y, Ding S, Zhu L, Zou G, Zhang X.Alexa Fluor 647 donkey anti-rabbit, 1:500, ab150075, Abcam) and 4′,6-diamidino-2-phénylindole (DAPI, ab228549, Abcam) were then used for detection. Images were acquired using an Olympus FV1200 confocal microscope

(248) Isolation and Characterization of Primary Retinal Microglia From the Human Post-mortem Eyes for Future Studies of Ocular DiseasesFrontiers in cellular neuroscienceJanuary 1, 2021Wang L, Qian Y, Che X, Jiang J, Suo J, Wang Z.1:1,000; Alexa Fluor 647 of goat anti-rabbit, Yeasen, 1:1,000) at room temperature for 1 h. DAPI (Abcam, #ab228549, 1:1,000) was used for staining nuclear DNA. The morphology of retinal microglia and their specific

(249) The subcellular redistribution of NLRC5 promotes angiogenesis via interacting with STAT3 in endothelial cellsTheranosticsJanuary 1, 2021Xu X, Shi Y, Luan P, Kou W, Li B, Zhai M, You S, Yu Q, Zhuang J, Jian W, et al.sectioning at 8 mm thickness. Sections were incubated in primary antibodies (Table S1.1). DAPI 1:5000 (Cat# ab228549, Abcam) was used as a nuclear counterstain. Tissue was visualized using objective on Nikon Eclipse TE-2000U

(250) Nonrandom DNA Segregation Detection under Replication StressSTAR ProtocolsDecember 30, 2020Sisi Chen, Fang Ji, Meichun Xing, Gaoying Chen, Jiaqian Li, Xinwei Geng, Chao Zhang, Madiha Zahra Syeda, Songmin Yingfrom Sigma (T8787); BSA can also be obtained from Sigma (A1933); DAPI can also be obtained from Abcam (ab228549). Step-By-Step Method Details CldU Administration Timing: 2 weeks Cells are treated with CldU (1

(251) Oxidative stress mediates thalidomide-induced pain by targeting peripheral TRPA1 and central TRPV4BMC biologyDecember 14, 2020De Logu F, Trevisan G, Marone IM, Coppi E, Padilha Dalenogare D, Titiz M, Marini M, Landini L, Souza Monteiro de Araujo D, Li Puma S, et al.at RT. Slides were then coverslipped with mounting medium with 4′,6-diamidino-2-phenylindole (DAPI, #ab228549, Abcam). Lumbar (L4–L6) spinal cord collected from perfused mice was placed overnight at 4 °C in 10%

(252) Fruquintinib inhibits VEGF/VEGFR2 axis of choroidal endothelial cells and M1-type macrophages to protect against mouse laser-induced choroidal neovascularizationCell death & diseaseNovember 27, 2020Liu X, Guo A, Tu Y, Li W, Li L, Liu W, Ju Y, Zhou Y, Sang A, Zhu M.anti-F4/80 antibody (#ab105156, Abcam, USA) antibodies, and 4′, 6-diamidino-2-phenylindole (DAPI; #ab228549, Abcam) were used in the immunofluorescence. Hematoxylin–eosin stain On day 7, following euthanasia,

(253) Noise Exposures Causing Hearing Loss Generate Proteotoxic Stress and Activate the Proteostasis NetworkCell reportsNovember 24, 2020Jongkamonwiwat N, Ramirez MA, Edassery S, Wong ACY, Yu J, Abbott T, Pak K, Ryan AF, Savas JN.were then rinsed three times in PBS for 15 minutes and stained with DAPI Staining Solution (Abcam, ab228549) for 5 minutes prior to mounting in Fluoromount-G (Thermo Scientific, cat. 00–4958-02). Tissues were

(254) Laquinimod Inhibits Inflammation-Induced Angiogenesis in the CorneaFrontiers in MedicineNovember 10, 2020Zuohong Li, Jianping Chen, Lei Lei, Nan Jiang, Yanling Zhu, Yu Jia, Yehong Zhuo, Wenru SuAlexa Fluor 555 (Cell Signaling Technology, Germany, 1:1000). After counterstaining with DAPI (Abcam, ab228549), the sections were mounted with antifade mounting medium (Beyotime, Shanghai, China). The sections

(255) Enterococcus faecalis Induces Differentiation of Immune-Aberrant Dendritic Cells from Murine Bone Marrow-Derived Stem CellsInfection and ImmunityOctober 19, 2020Mohamed Mohamed Elashiry, Mahmoud Elashiry, Rana Zeitoun, Ranya Elsayed, Fucong Tian, Shehab Eldin Saber, Salma Hasan Elashry, Franklin R. Tay, Christopher W. Cutler(Thermo Fisher Scientiﬁc), and the nuclei were counterstained with 4=,6-diamidino-2-phenylindole (DAPI) (ab228549; Abcam, Burlingame, CA) and visualized using an inverted confocal microscope (LSM 780; Carl Zeiss AG, Oberkochen,

(256) P2X7 Receptor (P2X7R) of Microglia Mediates Neuroinflammation by Regulating (NOD)-Like Receptor Protein 3 (NLRP3) Inflammasome-Dependent Inflammation After Spinal Cord InjuryMedical science monitor : international medical journal of experimental and clinical researchSeptember 21, 2020Fan X, Ma W, Zhang Y, Zhang L.Thermo Fisher Scientific, Shanghai, China) at room temperature for 2 h in the dark and then incubated with DAPI (1: 1000, ab228549, Abcam, Shanghai, China) at room temperature for 3 min. Sections were observed under a confocal laser

(257) Silencing glioma‑associated oncogene homolog 1 suppresses the migration and invasion of hepatocellular carcinoma in vitroOncology LettersSeptember 11, 2020Zeming Hu, Fangfang Xie, Ang Hu, Mengjing Xu, Yuwen Liu, Jiankang Zhang, Jianbo Xiao, Yunlei Song, Jianing Zhong, Bin ChenAfter washing three times with PBST, the cell nuclei were stained with DAPI (1 µg/ml, Abcam, cat. no. ab228549) for 10 min at room temperature in the dark and detected with a confocal laser scanning microscope (LSM

(258) Functional recovery with histomorphometric analysis of nerves and muscles after combination treatment with erythropoietin and dexamethasone in acute peripheral nerve injuryPLOS ONESeptember 3, 2020Jung Il Lee, Jeong Min Hur, Jooyoung You, Duk Hee Leeperipheral nerve injury 1:200, ab31851, Abcam); blue (40 ,6-diamidino-2-phenylindole [DAPI] 1:1000, ab228549, Abcam); control, staining without primary antibody; uninjured, normal nerve; saline, nerve treated

(259) TRPA1 mediates damage of the retina induced by ischemia and reperfusion in miceCell death & diseaseAugust 15, 2020Souza Monteiro de Araújo D, De Logu F, Adembri C, Rizzo S, Janal MN, Landini L, Magi A, Mattei G, Cini N, Pandolfo P, et al.Sections were coverslipped using a water-based mounting medium with 4′6′-diamidino-2-phenylindole (DAPI, #ab228549, Abcam, Cambridge, UK). The analysis of negative controls (nonimmune serum) was simultaneously performed

(260) Microdomains form on the luminal face of neuronal extracellular vesicle membranesScientific reportsJuly 20, 2020Matthies D, Lee NYJ, Gatera I, Pasolli HA, Zhao X, Liu H, Walpita D, Liu Z, Yu Z, Ioannou MS.RRID:AB_2313773) and goat anti-mouse alexafluor488 (Invitrogen, A11001) and stained with DAPI (Abcam, ab228549). Neurons were routinely monitored for viability and mycoplasma contamination by imaging nuclei stained

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6/17/2020This DAPI Binds Strongly to Nuclei Samantha LanjewarIt stains nuclei well and is easy to use.

DAPI Staining Solution from Abcam

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DAPI Staining Solution from Abcam

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