DAPI Staining Solution ab228549 from Abcam

Product Specs
ItemDAPI Staining Solution
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab228549
Quantity2 mL
Sample TypeAdherent cells, Suspension cells
TypeStain

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(301) High-throughput three-dimensional spheroid tumor model using a novel stamp-like toolJournal of Tissue EngineeringNovember 29, 2019Wei Liao et al.spheroids were incubated with Phalloidin-iFluor 488 Reagent (ab176753; Abcam, Cambridge, UK) and DAPI (ab228549; Abcam) for 2 h in the dark and then washed 3 times with PBS. Fluorescence images were obtained using

(302) Overexpressed vascular endothelial growth factor in adipose derived stem cells attenuates fibroblasts and skin injuries by ultraviolet radiationBioscience reportsJuly 31, 2019Xie X, Wang Y, Xia Y, Mao Y.was diluted to 1:1000 and mixed in bovine serum albumin. The cells were counter-stained with DAPI (ab228549, Abcam, San Francisco, MA, U.S.A.) and diluted in PBST to 2 μg/ml and held for 20 min. The cells were mounted

(303) Ghrelin regulates sepsis‑induced rat acute gastric injuryMolecular medicine reportsJune 1, 2019Li B, Lin Q, Guo H, Liu L, Li Y.signal according to the manufacturer's protocol. Nuclei were marked using DAPI (10 µg/ml; cat. no. ab228549; Abcam) at room temperature for 5 min in the dark. RNA extraction and semi-quantitative polymerase chain reaction

(304) Downregulation of endothelial transient receptor potential vanilloid type 4 channel underlines impaired endothelial nitric oxide-mediated relaxation in the mesenteric arteries of hypertensive ratsPhysiological ResearchApril 30, 2019A Boudaka, M Al-Suleimani, I Al-Lawati, H Baomar, S Al-Siyabi, F Zadjali% O2 and 5 % CO2. Optimal Cutting Temperature compound was purchased from VWR chemicals (UK). DAPI (ab228549), BSA (ab64009), primary (Rabbit polyclonal to TRPV4; ab39260) and secondary antibodies (Goat Anti-Rabbit

(305) Pleural Effusion Aspirate for Use in 3D Lung Cancer Modeling and Chemotherapy ScreeningACS Biomaterials Science & EngineeringApril 8, 2019Andrea Mazzocchi et al.pan-cytokeratin (CK, 1:200, ab215838, Abcam), P63 (1:200, CM163, Biocare Medical, Pacheco, CA), and DAPI (1:300, ab228549, Abcam). For sample 7-23-17, primary antibodies Mucin5AC (1:200, ab3649), CK7(1:200, ab9021), and CK20

(306) Mesenchymal stem cells with downregulated Hippo signaling attenuate lung injury in mice with lipopolysaccharide‑induced acute respiratory distress syndromeInternational journal of molecular medicineMarch 1, 2019Li L, Dong L, Zhang J, Gao F, Hui J, Yan J.A fluorescent fluorescein isothiocyanate/tetramethylrhodamine-labeled secondary antibody (cat. no. ab228549; 1:1,000; Abcam) was added, incubated at 25°C for 1 h, and washed with PBS. Subsequently, 4′,6-diamidino-2-phenylin-dole

(307) Essential Role Of High Glucose-Induced Overexpression Of PKCβ And PKCδ In GLP-1 Resistance In Rodent CardiomyocytesDiabetes, metabolic syndrome and obesity : targets and therapyJanuary 1, 2019Pan X, Chen J, Wang T, Zhang M, Wang H, Gao H.USA 15596018 TUNEL kit (green) TUNEL kit (red) Takara, Japan Beyotime, China MK500 C1090 DAPI Abcam, UK ab228549 Caspase-3 assay kit ROS assay kit Clontech, CA Abcam, UK 630217 ab113851 Western Blot Detection

(308) An integrated analysis revealing the angiogenic function of TP53I11 in tumor microenvironmentHeliyonJanuary 1, 0001"Bai W, Ren JS, Li KR, Jiang Q."HUVECs were stained with rhodamine-conjugated phalloidin (Abcam, ab235138, 1:1000) and DAPI (Abcam, ab228549, 1:1000) to visualize the actin cytoskeleton and nuclei, respectively. 2.14 Western blot Proteins

(309) SARS-CoV-2 nucleocapsid protein adheres to replication organelles before viral assembly at the Golgi/ERGIC and lysosome-mediated egressScience advancesJanuary 1, 0001"Scherer KM, Mascheroni L, Carnell GW, Wunderlich LCS, Makarchuk S, Brockhoff M, Mela I, Fernandez-Villegas A, Barysevich M, Stewart H, et al."meant for expansion microscopy were counterstained with DAPI (4′,6-diamidino-2-phenylindole) (Abcam, ab228549) diluted 1:1000 in PBS for 15 min at RT and mounted on Fisherbrand glass microscope slides (Fisher Scientific,

(310) Biological and clinical significance of the AGE-RAGE axis in the aggressiveness and prognosis of prostate cancerJournal of food and drug analysisJanuary 1, 0001"Khoo SH, Wu PR, Yeh KT, Hsu SL, Wu CH."Scientific Hoechst 33342,62249,Thermo Fisher Propidium iodide (PI),P3566,Thermo Fisher Scientific DAPI,ab228549,Abcam HMGB1 recombinant protein,ab167718,Abcam Listed of chemicals used in this study

(311) Label-free macrophage phenotype classification using machine learning methodsScientific reportsJanuary 1, 0001"Hourani T, Perez-Gonzalez A, Khoshmanesh K, Luwor R, Achuthan AA, Baratchi S, O'Brien-Simpson NM, Al-Hourani A."#PKH67GL) as per the manufacturer instructions, and cell nuclei were stained with 0.5 μg/mL DAPI (Abcam, # ab228549) for 5 min at room temperature. Cells were washed again with PBS and the immunofluorescent pictures

(312) Dysregulated miR-124 mediates impaired social memory behavior caused by paternal early social isolationTranslational psychiatryJanuary 1, 0001"Chen S, Ding S, Pang Y, Jin Y, Sun P, Li Y, Cao M, Wang Y, Wang Z, Wang T, et al."and #A31570) for 2 hours at 25°C. The nuclei were stained by DAPI staining solution (1:1000; Abcam, #ab228549) for 10 minutes at 25°C. Western blot Frozen mPFC, hippocampal and amygdala brain tissues were lysed

(313) Lipid Deposition Profiles Influence Foreign Body Responses"Advanced materials (Deerfield Beach, Fla.)"January 1, 0001"Schreib CC, Jarvis MI, Terlier T, Goell J, Mukherjee S, Doerfert MD, Wilson TA, Beauregard M, Martins KN, Lee J, et al."PBST for 3 washes (5 minutes per wash), the mounted slides were incubated with DAPI (1:1000; Abcam ab228549) in 1x PBST at room temperature for 5 minutes. After final washing steps with 1x PBST for 3 washes (5

(314) "Repetitive Transcranial Magnetic Stimulation Reduces Depressive-like Behaviors, Modifies Dendritic Plasticity, and Generates Global Epigenetic Changes in the Frontal Cortex and Hippocampus in a Rodent Model of Chronic Stress"CellsJanuary 1, 0001"Meneses-San Juan D, Lamas M, Ramírez-Rodríguez GB."anti-5-methylcytosine (1:500, Cat: ab214727, Abcam). 4′,6-diamidino-2-phenylindole (DAPI), (1:1000, Cat: ab228549, Abcam) was used to stain the nuclei of the hippocampal dentate gyrus cells. Images were acquired using

(315) "A physiologically relevant, estradiol-17β [E2]-responsive in vitro tissue-engineered model of the vaginal epithelium for vaginal tissue research"Neurourology and urodynamicsJanuary 1, 0001"Shafaat S, Mangir N, Chapple C, MacNeil S, Hearnden V."Abcam) for 1 h at RT and counterstained with 4′,6‐diamidino‐2‐phenylindole (λex 359 nm, λem 457 nm) (ab228549) (1:800 dilution; Abcam), mounted with DPX, and imaged with an epifluorescence microscope (Olympus IX73). 2.8.1

(316) A conditional null allele of Dync1h1 enables targeted analyses of dynein roles in neuronal length sensingJournal of cell scienceJanuary 1, 0001"Di Pizio A, Marvaldi L, Birling MC, Okladnikov N, Dupuis L, Fainzilber M, Rishal I."donkey anti-rabbit-IgG conjugated to Alexa Fluor 647 (1:1000; Jackson ImmunoResearch) and DAPI (Abcam, ab228549, 1:5000). After three washes in 1× PBS, coverslips were rinsed in ddH2O and mounted with Fluoromount-GTM. Immunohistochemistry

(317) The protease SPRTN and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instabilityCell reportsJanuary 1, 0001"Ruggiano A, Vaz B, Kilgas S, Popović M, Rodriguez-Berriguete G, Singh AN, Higgins GS, Kiltie AE, Ramadan K.",Santa Cruz Biotechnology,Cat#sc-284628 Hoechst 33258,Sigma-Aldrich,Cat#B2883 DAPI staining solution,Abcam,Cat#ab228549 Propidium Iodide,Sigma-Aldrich,Cat#P4864 KaryoMAX Colcemid solution,ThermoFisher Scientific,Cat#15212012 Proteinase K

(318) Cyp4a12-mediated retinol metabolism in stellate cells is the antihepatic fibrosis mechanism of the Chinese medicine Fuzheng Huayu recipeChinese medicineJanuary 1, 0001Ping DB, Sun X, Peng Y, Liu CH.then stained with Cy3 or FITC-conjugated secondary antibodies. After washing, DAPI (1:2000, Abcam, ab228549, UK) was used to stain the nuclei of the tissue. Images were taken with an Olympus confocal microscope,

(319) Macrophages induce gingival destruction via Piezo1-mediated MMPs-degrading collagens in periodontitisFrontiers in immunologyJanuary 1, 0001"Zhao T, Chu Z, Chu CH, Dong S, Li G, Wu J, Tang C."hour at room temperature under protection from light. Next, add 4’,6-diamidino-2-phenylindole (DAPI) (ab228549; Abcam, USA) to stain the nuclei and incubate for 10 minutes at room temperature in the dark. Finally,

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6/17/2020This DAPI Binds Strongly to Nuclei Samantha LanjewarIt stains nuclei well and is easy to use.

DAPI Staining Solution from Abcam

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DAPI Staining Solution from Abcam

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