DAPI Staining Solution ab228549 from Abcam

Product Specs
ItemDAPI Staining Solution
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab228549
Quantity2 mL
Sample TypeAdherent cells, Suspension cells
TypeStain

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(261) Caspase1/11 signaling affects muscle regeneration and recovery following ischemia, and can be modulated by chloroquineMolecular medicine (Cambridge, Mass.)July 8, 2020Sachdev U, Ferrari R, Cui X, Pius A, Sahu A, Reynolds M, Liao H, Sun P, Shinde S, Ambrosio F, et al.microtubule associated protein 1A/1B-light chain 3 (LC3; #L8918, Sigma,Sigma-Aldrich, St. Louis, MO) DAPI (#ab228549; Abcam), Cy-3-conjugated secondary antibodies (#ab6939;Abcam); antibody to myosin heavy chain fast (#ab51263)

(262) Mechanically Robust, Softening Shape Memory Polymer Probes for Intracortical RecordingMicromachines (Basel)June 25, 2020Allison M. Stiller, Joshua O. Usoro, Jennifer Lawson, Betsiti Araya, María Alejandra González-González, Vindhya R. Danda, Walter E. Voit, Bryan J. Black, Joseph J. Pancrazioanti-mouse Alexa Fluor 555 (1:200, Abcam, ab150118), along with 0.06% concentration(0.06%, DAPI Abcam,Abcam, ab228549). Sections were then 3 times for 10for min PBS The The PBS PBS was (0.06%, ab228549). Sections werewashed then

(263) Effects and mechanism of Lanthanum Citrate on the proliferation and apoptosis of hepatocellular carcinoma cell line SMMC-7721The Turkish Journal of GastroenterologyMay 11, 2020Bin Chen, Department of Hepatobiliary Surgery, the First Affiliated Hospital of Gannan Medical University, Ganzhou, China, Zeming Hu, Biao Chen, Bofei Li, Gannan Medical University, Ganzhou, China, Gannan Medical University, Ganzhou, China, Department of General Surgery, Rucheng County People’s Hospital, Chenzhou, China1 h. The cells were washed three more times with PBST and then incubated with DAPI (1µg/ ml, Abcam, ab228549, USA) and detected with a Confocal Laser Scanning Microscope (Carl Zeiss LSM 880). Statistical analysis SPSS

(264) miR‑217‑5p regulates myogenesis in skeletal muscle stem cells by targeting FGFR2Molecular Medicine ReportsMay 7, 2020Menghai Zhu, Gang Chen, Yi Yang, Jiantao Yang, Bengang Qin, Liqiang Guwith 2% goat serum (Gibco; Thermo Fisher Scientific, Inc.) for 1 h at room temperature. DAPI (cat. no. ab228549, Abcam) was used to identify the nuclei. The cells were incubated with primary antibodies overnight

(265) ZEB2-AS1 Accelerates Epithelial/Mesenchymal Transition Through miR-1205/CRKL Pathway in Colorectal CancerCancer Biotherapy and RadiopharmaceuticalsMarch 1, 2020Yinghao Jiang, Guangming Liu, Wei Ye, Jianjin Xie, Chunfa Shao, Xiaowei Wang, Xia LiIgG (Vector Labs), was added. The cells were counterstained with 4¢,6¢-diamidino-2phenylindole (DAPI; ab228549, Abcam, Cambridge, United Kingdom). All the above procedures were carried out in a dark room. The samples

(266) rTMS Regulates the Balance Between Proliferation and Apoptosis of Spinal Cord Derived Neural Stem/Progenitor CellsFrontiers in Cellular NeuroscienceJanuary 28, 2020Chen-Guang Zhao, Jie Qin, Wei Sun, Fen Ju, Yong-Lin Zhao, Rui Wang, Xiao-Long Sun, Xiang Mou, Hua YuanA-11035, Thermo Fisher Scientific, Waltham, MA, USA) and 4’,6-diamidino-2-phenylindole (DAPI; 1:1,000, ab228549, Abcam, Cambridge, UK) for 45 min at room temperature. Finally, samples were washed with phosphate buffered

(267) GCNA Interacts with Spartan and Topoisomerase II to Regulate Genome StabilityDevelopmental cellJanuary 6, 2020Dokshin GA, Davis GM, Sawle AD, Eldridge MD, Nicholls PK, Gourley TE, Romer KA, Molesworth LW, Tatnell HR, Ozturk AR, et al.dilutions at room temperature for 2 hours, washed twice in PBST, and secondary antibodies and DAPI (Abcam ab228549) were applied at 1:1000 dilution for 1 hours in darkness prior to mounting and imaging. Live cell imaging

(268) Evidence of SARS-CoV2 Entry Protein ACE2 in the Human Nose and Olfactory BulbCells, tissues, organsJanuary 1, 2020Klingenstein M, Klingenstein S, Neckel PH, Mack AF, Wagner AP, Kleger A, Liebau S, Milazzo A.After several washing steps, secondary antibodies were diluted 1:100 in PBS together with DAPI (Abcam, ab228549) and incubated for 45 min in room temperature away from daylight. The following secondary antibodies

(269) Fuzheng Huayu Recipe Prevented and Treated CCl4-Induced Mice Liver Fibrosis through Regulating Polarization and Chemotaxis of Intrahepatic Macrophages via CCL2 and CX3CL1Evidence-based complementary and alternative medicine : eCAMJanuary 1, 2020Zhang M, Liu HL, Huang K, Peng Y, Tao YY, Zhao CQ, Hu XD, Liu CH.donkey anti-rat IgG H&L (Alexa Fluor 647) (cat no: ab150155) were purchased from Abcam, UK. DAPI (cat no: ab228549) was purchased from Abcam, UK. Flow cytometric antibodies including anti-CD45 (cat no: 557659), anti-CD11b

(270) High-throughput three-dimensional spheroid tumor model using a novel stamp-like toolJournal of Tissue EngineeringNovember 29, 2019Wei Liao et al.spheroids were incubated with Phalloidin-iFluor 488 Reagent (ab176753; Abcam, Cambridge, UK) and DAPI (ab228549; Abcam) for 2 h in the dark and then washed 3 times with PBS. Fluorescence images were obtained using

(271) Overexpressed vascular endothelial growth factor in adipose derived stem cells attenuates fibroblasts and skin injuries by ultraviolet radiationBioscience reportsJuly 31, 2019Xie X, Wang Y, Xia Y, Mao Y.was diluted to 1:1000 and mixed in bovine serum albumin. The cells were counter-stained with DAPI (ab228549, Abcam, San Francisco, MA, U.S.A.) and diluted in PBST to 2 μg/ml and held for 20 min. The cells were mounted

(272) Ghrelin regulates sepsis‑induced rat acute gastric injuryMolecular medicine reportsJune 1, 2019Li B, Lin Q, Guo H, Liu L, Li Y.signal according to the manufacturer's protocol. Nuclei were marked using DAPI (10 µg/ml; cat. no. ab228549; Abcam) at room temperature for 5 min in the dark. RNA extraction and semi-quantitative polymerase chain reaction

(273) Downregulation of endothelial transient receptor potential vanilloid type 4 channel underlines impaired endothelial nitric oxide-mediated relaxation in the mesenteric arteries of hypertensive ratsPhysiological ResearchApril 30, 2019A Boudaka, M Al-Suleimani, I Al-Lawati, H Baomar, S Al-Siyabi, F Zadjali% O2 and 5 % CO2. Optimal Cutting Temperature compound was purchased from VWR chemicals (UK). DAPI (ab228549), BSA (ab64009), primary (Rabbit polyclonal to TRPV4; ab39260) and secondary antibodies (Goat Anti-Rabbit

(274) Pleural Effusion Aspirate for Use in 3D Lung Cancer Modeling and Chemotherapy ScreeningACS Biomaterials Science & EngineeringApril 8, 2019Andrea Mazzocchi et al.pan-cytokeratin (CK, 1:200, ab215838, Abcam), P63 (1:200, CM163, Biocare Medical, Pacheco, CA), and DAPI (1:300, ab228549, Abcam). For sample 7-23-17, primary antibodies Mucin5AC (1:200, ab3649), CK7(1:200, ab9021), and CK20

(275) Mesenchymal stem cells with downregulated Hippo signaling attenuate lung injury in mice with lipopolysaccharide‑induced acute respiratory distress syndromeInternational journal of molecular medicineMarch 1, 2019Li L, Dong L, Zhang J, Gao F, Hui J, Yan J.A fluorescent fluorescein isothiocyanate/tetramethylrhodamine-labeled secondary antibody (cat. no. ab228549; 1:1,000; Abcam) was added, incubated at 25°C for 1 h, and washed with PBS. Subsequently, 4′,6-diamidino-2-phenylin-dole

(276) Essential Role Of High Glucose-Induced Overexpression Of PKCβ And PKCδ In GLP-1 Resistance In Rodent CardiomyocytesDiabetes, metabolic syndrome and obesity : targets and therapyJanuary 1, 2019Pan X, Chen J, Wang T, Zhang M, Wang H, Gao H.USA 15596018 TUNEL kit (green) TUNEL kit (red) Takara, Japan Beyotime, China MK500 C1090 DAPI Abcam, UK ab228549 Caspase-3 assay kit ROS assay kit Clontech, CA Abcam, UK 630217 ab113851 Western Blot Detection

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6/17/2020This DAPI Binds Strongly to Nuclei Samantha LanjewarIt stains nuclei well and is easy to use.

DAPI Staining Solution from Abcam

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DAPI Staining Solution from Abcam

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