Fig 1: Il1rl2 silencing negatively affects organoid growth. (A) Representative pictures and (B) measurements of wild-type organoids treated with control siRNA or Il1rl2 siRNA for 6 d. Each dot represents an individual organoid, organoid cultures were produced from 3 different mice and plated in three wells for each experimental condition. (Scale bar: 200 μm.) Comparisons between control siRNA and Il1rl2 siRNA organoids were performed using Student t test. (C–H) Gene expression analysis of Il1rl2 (C), Lgr5 (D), Fzd5 (E), Ephb2 (F), Olfm4 (G), and Lyz1 (H) in wild-type organoids treated with either control siRNA or Il1rl2 siRNA for 6 d. Comparisons between control siRNA and Il1rl2 siRNA organoids were performed using Student t test. (I) Representative pictures and (J) measurements of wild-type and Il1rapl1-deficient organoids treated with control siRNA, Il1rl2 siRNA, vehicle, or IL-38 for 6 d. Each dot represents an individual organoid; organoid cultures were produced from 3 different mice and plated in three wells for each experimental condition. (Scale bar: 200 μm.) Comparisons between the eight experimental groups were performed using one-way ANOVA. *P < 0.05; **P < 0.01; ****P < 0.0001. Mean ± SEM.