Fig 1: Generation of MDA-MB-231 cells stably overexpressing miR-4638-3p. Total RNA was isolated from MDA-MB-231 cells (MDA), stable MDA-MB-231 cells overexpressing empty vector (MDA-EV C2), and MDA-MB-231 cells overexpressing miR-4638 (MDA-miR C4) cells. The isolated RNA was subjected to cDNA synthesis, followed by qPCR analysis with primers for miR-4638-3p. * a substantial increase compared to parental MDA and stable MDA-EV C2 cells