Fig 1: Evaluation of the methylation status of 30 CpG sites ~1000bp upstream of the miR-200c/miR-141 cluster on murine chromosome 6 as determined by targeted bisulfite sequencingEach CpG site is represented by an individual pie and percentage of CpG sites methylated are indicated by the amount of shading; <5% methylation, , 5-25% methylation, , 26-50% methylation, , 51-75% methylation, , and >75% methylation, (A). miR-200c and miR-200b (first miRNA in miR-200c/141 or miR-200b/200a/429 cluster) levels in RJ423 cells treated daily with DMSO or 3μM of the DNA methyltransferase inhibitor, 5-aza-2′-deoxycytidine (5-aza) for 72hrs (B). Each bar represents the mean ± SEM of 3 independent trials, *p<0.05.
Fig 2: Expression (mean ± SEM) of miR-200a, miR-200b, miR-200c, miR-141 and miR-429 in RJ345 cells, RJ423 cells containing the empty vector control (RJ423EV) or RJ423 cells containing miR-200c (RJ423200c) as determined by Taqman RT-PCRThe expression of each miRNA was normalized to the levels of SnoRNA202 and SnoRNA234. *indicates significant difference (p<0.05) in RJ4232EV and RJ423200c cells compared to RJ345 cells while **indicates significant differences (p<0.05) between RJ423EVand RJ423200c cells. Each cell line represents n≥3.
Supplier Page from OriGene Technologies for Mir200c Mouse MicroRNA Expression Plasmid (MI0000694)