Fig 1: Activation Piezo1 inhibits hippo/YAP signaling activation. (a and b) Western blot and semi-quantitative analysis of protein expression. (c) mRNA levels of hippo signaling components measured by RT-PCR. (d) Western blot and semi-quantitative analysis of protein expression of YAP in the cytoplasm and nucleus. Data were expressed as mean ± SD. ***p < 0.001.
Fig 2: Piezo1 knockdown inhibited OC growth in vivo. (a) Tumor volume of A-1847 xenografts with or without Piezo1 knockdown. n = 6 for each group. (b) Piezo1 mRNA levels of tumor collected from different groups. Data were expressed as mean ± SD. *p < 0.05.
Fig 3: Knockdown of Piezo1 gene expression suppressed cell migration in A-1847 cells. (a) mRNA and (b-c) protein expression of Piezo1 in A-1847 cells after knockdown using Piezo1-shRNA. (d) Wound disclosure rate of A-1847 cells with or without Piezo1 knockdown. Data were expressed as mean ± SD. **p < 0.01.
Supplier Page from OriGene Technologies for FAM38A (PIEZO1) Human shRNA Plasmid Kit (Locus ID 9780)