Fig 1: Targets of dysregulated miRNAs in MDD patients (mRNA relative quantification values).The gene expression values were determined using Affymetrix U133 Plus 2.0 array and compared HIV/MDD vs HIV alone from frontal cortex tissue from 6 patients in each group; one-way ANCOVA compared the two groups and were different at p<0.05 significance are listed in Table S1. The gene expression is plotted for those with 3′UTR seed sequences for miRNAs that showed highly significant target bias. The direction of the dysregulation of the miRNA is indication by the arrow; for miR-367, miR-136, and miR-125a, the majority of targets are dysregulated in accordance with the miRNAs; while for miR-502, the majority of targets are anticorrelated. ↑ or ↓ indicate the miRNA was upregulated or downregulated, respectively, in the array.
Fig 2: (A) Decreased protein production of IFITM3 in miR-125a overexpressing neuronal cells and (B) decreased secretion of sTNFR-1A in miR-22 overexpressing neuronal cultures.
Fig 3: Lentiviral overexpression of (A) miR-22 and (B) miR-125a in primary neuronal cultures.
Supplier Page from OriGene Technologies for Mir125a Mouse MicroRNA Expression Plasmid (MI0000151)