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Cloning and Expression
Library Construction
cDNA Synthesis
RNA
RNA
Alt-R™ L.b. Cas12a crRNA
Alt-R™ L.b. Cas12a crRNA from Integrated DNA Technologies
Be the first to write a review!
Citations:
(6)
Supplier Page
Supplier Page from
Integrated DNA Technologies for
Alt-R™ L.b. Cas12a crRNA
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Description
Customer-defined crRNA that will bind to 21–24 bases opposite to the TTTV, PAM sequence
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Alt-R™ L.b. Cas12a crRNA
Company
Integrated DNA Technologies
Price
Supplier Page
View Company Product Page
Catalog Number
AltRLb
Applications
CRISPR
Type
Oligonucleotides
Add to Compare List
Integrated DNA Technologies
1710 Commercial Park
Coralville
,
Iowa
52241
United States
Phone
: 800-328-2661
Company Profile
Website:
http://www.idtdna.com
Citations (6)
(1)
Knockout of nAChR subunits in spider mites and their phytoseiid predators confers spinosyn cross-resistance and reveals a conserved mode of action in mites
Insect Biochemistry and Molecular Biology
April 1, 2026
Antonio Mocchetti, Pieter Steelant, Mahboubeh Hosseinkhani, Sander De Rouck, Jahangir Khajehali, Thomas Van Leeuwen
Amicon® Ultra Centrifugal Filter (30 kDa cut-off). sgRNAs (
Alt-R™ CRISPR/Cas9 sgRNA
,
IDT
) and crRNA (
Alt-R™ L.b. Cas12a crRNA
,
IDT
) were dissolved in TE buffer to reach a final concentration of 10 μg/μL and 4 μg/μL, respectively.
(2)
APOA1 binding protein promotes lymphatic cell fate and lymphangiogenesis by relieving caveolae-mediated inhibition of VEGFR3 signaling
Nat Commun
October 21, 2025
Jun-Dae Kim, Surbhi Chaudhary, Weiqing Chen, Jonathan Astin, Philip S. Crosier, Pengchun Yu, John P. Cooke, Henry J. Pownall, Hugo J. Bellen, Nhat-Tu Le,
et al.
ATGACGATGTGGTGAAGGTAGGA-3′. The
CRISPR
RNA (crRNA) was ordered from
Integrated DNA Technologies, Inc
. (
Alt-R™ L.b. Cas12a crRNA
2 nmol, USA) and diluted as 20 mM in RNase-free water. To make RNP mixture, 1 µl crRNA (2 µM) and 0.5
(3)
DNA-Gold Nanoparticle Dumbbells: Synthesis and Nanoscale Characterization
Nanomaterials (Basel)
October 17, 2025
Esraa Hijaze, Liat Katrivas, Zakhar Reveguk, Shachar Richter, Alexander B. Kotlyar
UV–vis
spectroscopy
. 2.5. Preparation of
CRISPR
-Cas12a
Alt-R™ L.b. Cas12a (Cpf1) Ultra
and gRNA (
Alt-R™ L.b. Cas12a crRNA
) were purchased from
Integrated DNA Technologies
(IDT, Coralville, IA, USA). The sequences (5′→3′) of
(4)
Divergence in neuronal signaling pathways despite conserved neuronal identity among Caenorhabditis species
Current Biology
June 23, 2025
Itai Antoine Toker, Lidia Ripoll-Sánchez, Luke T. Geiger, Antoine Sussfeld, Karan S. Saini, Isabel Beets, Petra E. Vértes, William R. Schafer, Eyal Ben-David, Oliver Hobert
E,
Sigma-Aldrich
,Cat#
P8811
Alt-R S.p. Cas9 Nuclease V3
,
IDT
,Cat# 1081059
Alt-R CRISPR-Cas9 tracrRNA
,
IDT
,Cat#
1072532
Alt-R™ L.b. Cas12a crRNA
,
IDT
,Cat#
10007922
Q5® High-Fidelity DNA Polymerase
,
New England Biolabs
,Cat#
M0491
Lambda Exonuclease
,
New England Biolabs
(5)
Protocol for CRISPR-based endogenous protein tagging in mammalian cells
STAR Protocols
December 20, 2024
Yu-Xi Xiao, Jiarun Wei, Jason Moffat
buffer,
NEB
,
B6002S
BbsI
,
NEB
,
R0539S
MyTaq HS red mix
,
Bioline
,
BIO-25047
Alt-R L.b. Cas12a (Cpf1) Ultra
,
IDT
,
10007922
Alt-R L.b. Cas12a crRNA
,
IDT
,N/A ,, Critical commercial
assays
,Critical commercial
assays
,Critical commercial
assays
,, GeneArt
(6)
High-throughput methodology to identify CRISPR-generated Danio rerio mutants using fragment analysis with unmodified PCR products
Developmental Biology
April 1, 2022
Sarah Colijn, Ying Yin, Amber N. Stratman
cluap1 locus. We will outline the workflow and our results here. 2. Materials 2.1. For
injection
Alt-R® L.b. Cas12a crRNA
[custom;
IDT
]EnGen® Lba Cas12a (Cpf1) [M0653; NEB]2M KCl solution*
Phenol red solution
[
P0290
;
Sigma
]Petri
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Alt-R™ L.b. Cas12a crRNA from Integrated DNA Technologies
Supplier Page
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