Description
Invitrogen TrueGuide Synthetic tracrRNA is a 72-bp gRNA scaffold that hybridizes with TrueGuide Synthetic crRNAs to form a gRNA complex that directs Cas9 nuclease to a specific editing site within the genome. The performance of this two-piece gRNA system is boosted by the addition of 2’O-methyl analogs and 3’ phosphorothioate internucleotide linkages. These modifications enhance editing efficiency by increasing binding to the target site and inhibiting nuclease degradation, respectively. The modifications are incorporated into the terminal three nucleotides on the 5’ and 3’ ends of both the TrueGuide Synthetic tracrRNA and the TrueGuide Synthetic crRNAs. TrueGuide Synthetic tracrRNA is available in three package sizes: 5 nmol, 20 nmol, and 100 nmol