Fig 1: Gata4, Mef2c, and Tbx5 expression in the beating and non-beating groups. (a) Left three panels, gene expression levels in the beating and non-beating group. Right panel, gene expression levels in the beating and non-beating group relative to that of β-actin. (b) Representative immunofluorescence images of a cluster of round cells expressing Gata4 and Mef2c on day 14. (c) Phase-contrast and immunofluorescence images of beating cells expressing cardiac troponin T (cTnT) and Mef2c on day 28. The arrowhead indicates a beating cell. Scale bar, 100 µm. DAPI, 4′,6-diamidino-2-phenylindole, ** p < 0.01; **** p < 0.0001 by t-test or ANOVA and Tukey’s post hoc test. (d) The frequency of round cells expressing Gata4 and Mef2c (10 round cells were examined per experiment, n = 13). (e) The frequency of beating cells expressing Mef2c and cTnT (15 beating cells were analysed per experiment, n = 4).
Fig 2: Overexpression of SVF with Mef2c augments the number of round cells and their transdifferentiation into CMs. (a–d) Phase-contrast images of SVF on day 28 after transfection on day 5 (a, control; b, Gata4; c, Mef2c; d, Tbx5). Arrowheads indicate round cells; lv, lentivirus vector. (e) mRNA level of Mef2c, determined by qRT-PCR, on day 28 in SVFs transfected with the control vector and Mef2c-lv (left), or scrambled shRNA and shMef2c-lv (right), on day 5, respectively. shRNA, short hairpin RNA; shMef2c, shRNA targeting Mef2c. (f) Number of round cells on day 14 after introducing SVF with none (none) or the control, Gata4, Mef2c, shMef2c, or Tbx5 lentivirus vector. (g) mRNA level of cardiac troponin T by qRT-PCR on day 28 in SVF transfected with the control, Gata4, Mef2c, shMef2c, or Tbx5. (h) Relationship between the mRNA levels of Mef2c and cardiac troponin T in the SVF transduced with Mef2c. R, Pearson’s product-moment correlation coefficient. The linear interpolation formula is displayed. Scale bar = 100 µm. * p < 0.05; ** p < 0.01; **** p < 0.0001 by ANOVA and Tukey’s post hoc test.
Supplier Page from OriGene Technologies for Mef2c Mouse shRNA Lentiviral Particle (Locus ID 17260)