PureYield Plasmid Miniprep System from Promega

Product Specs
ItemPureYield Plasmid Miniprep System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA1223
Time> 10 minutes
Volume
- If higher yields are desired-1.5–3.0ml of culture
- low-copynumber plasmids- 3ml culture volumes are recommended
Yieldpurify 1.5–7.5 µg of plasmid DNA
Quantity100 preps
Methodsilica membrane column
SourceBacterial
Nucleic Acid TypePlasmid DNA
Applicationspurification
Sample TypeBacterial culture

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Myosin IIA motor regulates attaching-effacing bacteria interactions with intestinal epitheliumGut MicrobesDecember 31, 2026Nayden G. Naydenov, Atif Zafar, Susana Lechuga, Ajay Zalavadia, Armando Marino-Melendez, John A. Hammer, Velia M. Fowler, Christine McDonald, Kenneth G. Campellone, Andrei I. Ivanovisolated from individual colonies using the PureYield Miniprep System (Promega, Madison, WI, cat. #A1222). The presence of the desired mutations was verified by DNA sequencing. For transfection, Cos-7 cells

(2) Loss of heterozygosity exposes germline mutations in complex I and drives Warburg metabolism in oncocytic carcinoma of the thyroidSci AdvJuly 3, 2026Celia de la Calle Arregui, Anderson R. Frank, Kelvin Mun, Jiwoong Kim, Kuntal Majmudar, Justin A. Bishop, Cheryl Lewis, Yang Xie, David G. McFaddengrown under ampicillin selection (100 μg/ml). Plasmid DNA was isolated using the Promega Miniprep Kit (#A1222). Lentiviruses encoding sgRNAs were produced as described above. TPC-1 cells were seeded at 4 × 105

(3) HCV-specific CD4+ T-cells are susceptible to HIV-1 and contribute to viral persistence during antiretroviral therapyEBioMedicineJuly 3, 2026Samaa T. Gobran, Armando Espinosa Ortiz, Omar Khedr, Jessica Dion, Laurence Raymond Marchand, Jonathan Dias, Amélie Cattin, Julie Bruneau, Nicolas Chomont, Marina B. Klein, et al.University, Philadelphia, PA. The plasmid was amplified through bacterial transformation by MiniPrep (Cat# A1223, Promega) and MaxiPrep (Cat# 12162, Qiagen) and viral stocks were produced in 293 T-cells (Cat#CRL-3216,

(4) FLI1 enhances the malignant phenotype of glioma cells and exerts immunomodulatory effects through feedback crosstalk with exonic circRNA FECR1 and interferon‑induced ISG15Int J Mol MedJuly 1, 2026Xue Wen, Xiaoyi Gu, Xu Yan, Naifei Chen, Lei Zhou, Hui Li, Andrew R. Hoffman, Wei Li, Ji-Fan Hu, Jiuwei CuiChemically Competent Cell. Plasmid DNA was collected by Wizard® Plasmid DNA Purification kit (cat. no. A1223; Promega Corporation) and sequenced. Statistical analysis The experimental data are presented as mean

(5) Endoplasmic Reticulum Geometry Dictates Neuronal Bursting via Calcium Store Refill Rates and Exposes Selective Neuronal VulnerabilityAdvanced ScienceJune 1, 2026Valentina Davi, Pierre Parutto, Yuyi Zhang, Tasuku Konno, Cecile Crapart, Raquel Pereira, John P. Franklin, Mosab Ali Awadelkareem, Daniel C Maddison, Michael J. Devine, et al.assays PureYield Plasmid Maxiprep System,Promega,A2393 PureYield Plasmid Miniprep System,Promega,A1222 Wizard SV Gel and PCR Clean‐Up System,Promega,A9282 NEBuilder HiFi DNA Assembly Master Mix,New England Biolabs

(6) CRISPR and compound screens in a novel ex vivo tissue model identify DDR1 and ETA as regulators of cancer cell invasionCellular & Molecular Biology LettersMay 6, 2026Junnan Liu, Wencheng Jiang, Xue Wang, Anca Azoitei, Hengchuan Liu, Gregoire Najjar, Kuangzheng Liu, Michael Karl Melzer, Stephan Stilgenbauer, Mohamed Elati, et al.plasmid DNA from each bacterial colony was isolated via the PureYield™ plasmid miniprep system (cat. no. A1223; Promega Corporation, Madison, USA). Plasmid DNA was sent for Sanger sequencing to determine the gRNA

(7) Workflow for Crystallographic Fragment Screening by Crystal Soaking for Protein Targets: A Case Study on Thioredoxin Glutathione Reductase From Schistosoma mansoniBio ProtocApril 20, 2026Lauro R. S. Neto, Bogar O. Montoya, José Brandão-Neto, Kelly Parker, Frank Von Delft, Nicholas Furnham, Raymond J. Owens, Floriano P. Silva-Jr(Sigma-Aldrich, catalog number: P8849) 48. PureYieldTM miniPlasmid Miniprep System (Promega, catalog number: A1223) 49. SG1 Crystallization Screen kit (Molecular Dimensions, catalog number: MD1-88) 50. SigmaMarker low-range

(8) Workflow for Crystallographic Fragment Screening by Crystal Soaking for Protein Targets: A Case Study on Thioredoxin Glutathione Reductase from Schistosoma mansoniBIO-PROTOCOLApril 20, 2026Lauro R. S. Neto, Bogar O. Montoya, José Brandão-Neto, Kelly Parker, Frank Von Delft, Nicholas Furnham, Raymond J. Owens, Floriano P. Silva-Jr(Sigma-Aldrich, catalog number: P8849) 48. PureYieldTM miniPlasmid Miniprep System (Promega, catalog number: A1223) 49. SG1 Crystallization Screen kit (Molecular Dimensions, catalog number: MD1-88) 50. SigmaMarker low-range

(9) The role of the VirB ligand CTP in the molecular mechanism of transcriptional anti-silencing in Shigella flexneriNucleic Acids ResearchFebruary 27, 2026Taylor M Gerson, Monika M A Karney, Helen J Wingprepared by isolating from DH10B cells using the Promega Pure Yield Plasmid Miniprep System (Promega #A1223). To prepare the DNA stock, 3 ml of culture was processed according to the manufacturer’s instructions

(10) Endogenous CD5L controls the metabolic and inflammatory state of human macrophagesFrontiers in ImmunologyJanuary 22, 2026Kashif Rasheed, Neda Nejati Moharrami, Erlend Bjørkøy Tande, Terje Espevik, Maria Yurchenko, Victor BoyartchukT4 DNA ligase reaction buffer (B0202) were from New England Biolabs, Ipswich, MA, USA. The PureYield Plasmid Miniprep System (A1222) was from Promega. Cells THP-1 human monocytic cell line (ATCC, TIB-202) was maintained in RPMI 1640

(11) HLA ‐Knockout: Enabling Allele‐Specific Knockout of HLA Class I Genes for Immunogenic EngineeringHLAJanuary 21, 2026Connor Mattivi, Shiyu Wang, Longtao Ji, Qian Xiao, Jian Cao(Thermo Fisher C737303) and amplified using the Promega PureYield Plasmid Miniprep System (Promega A1222). All constructs were verified by Sanger sequencing (Azenta Life Sciences). Guide sequences used are:

(12) De novo cysteine biosynthesis in Pseudomonas aeruginosa: Characterization of the two main cysteine synthase isoformsiScienceJanuary 16, 2026Rebecca Martedì, Jole Maria Lucia D’Angelo, Giulia Sassi, Marialaura Marchetti, Sarah Hijazi, Riccardo Percudani, Stefano Bettati, Barbara Campanini, Emanuela Frangipanipreps",Promega Italia Srl,Cat# A9281 "PureYield(TM) Plasmid Miniprep System, 100 preps",Promega Italia Srl,Cat# A1223 H2S/SULF calibration kit,Unisense A/S,Cat# CALKIT-H2S; Lot# 1.06 "Wizard(R) SV Gel and PCR Clean-up

(13) Investigating and correcting a rare pathogenic mutation in GDF11Human Genetics and Genomics AdvancesJanuary 15, 2026Sean T. Congdon, John Bennett, Rhoddean Opinya, Amara R. Agosto, Olivia Dossias, Christopher Kokko, Aime A. Levesque, Andrew O. Koob, Adam C. Silver, Cindy A. Thomas-Charles45567). Plasmid DNA was purified using the Promega PureYield Miniprep System (Promega, catalog no. A1222). Transformations were performed using New England Biolabs (NEB) 5-α Competent Escherichia coli (NEB,

(14) Development of a Porcine Cell Line Stably Expressing Ephrin-B2 for Nipah Virus Research and Diagnostic TestingMicrobiology and immunologyJanuary 1, 2026Zhang H, Saito A.C2992H) and extracted using the PureYield Plasmid Miniprep System (Promega, Madison, WI, USA, Cat# A1222). The plasmid sequence was verified using a SupreDye v3.1 Cycle Sequencing Kit (M&S TechnoSystems, Osaka,

(15) Functional Characterization of Glucokinase Variants to Aid Clinical Interpretation of Monogenic DiabetesInternational Journal of Molecular SciencesDecember 23, 2025Varsha Rajesh, Dora Evelyn Ibarra, Jing Yang, Haichen Zhang, Amy Barrett, Eleanor G. Kaplan, Amit Kumthekar, Fanny Sunden, Han Sun, Ananta Addala, et al.plasmid extraction (using Promega Pure Yield Plasmid Miniprep Kit, Promega, Madison, WI, USA, Cat: A1223). The region containing GCK was amplified, and the mutation introduced was validated by Sanger sequencing

(16) miR-219a-Robo1 axis acts as a conserved switch for slit-mediated commissural axon guidanceiScienceDecember 19, 2025Khot B, Suriyaarachchi H, Majumder T, Nathan A, Lu QR, Liu G.Dual-Luciferase® Reporter Assay System,Promega,Cat#E1910 PureLinkTM RNA Mini Kit,ThermoFisher,Cat#12183018A PureYieldTM Plasmid Miniprep Kit,Promega,Cat#A1222 HiSpeed Plasmid Midi Kit,Qiagen,Cat#12643 AccuScript High Fidelity 1st Strand cDNA Synthesis Kit,Agilent Technologies

(17) Origin and evolution of key enzymes in the anammox pathway revisitedGenome Biol EvolDecember 18, 2025Emil Hägglund, Alejandro Jiménez-González, Erik Hagström, Patrik Björkholm, Lionel Guy, Siv G E Anderssonresistant colonies were isolated and the plasmids were purified using PureYield mini prep kit (Promega, A1223) according to standard protocols. All plasmids were sequenced by Oxford nanopore technology at a minimum

(18) Loss of heterozygosity exposes germline mutations in complex I and drives Warburg metabolism in oncocytic carcinoma of the thyroidbioRxivDecember 9, 2025Celia de la Calle Arregui, Anderson R. Frank, Kelvin Mun, Jiwoong Kim, Kuntal Majmudar, Justin A. Bishop, Cheryl Lewis, Yang Xie, David G. McFaddengrown under ampicillin selection (100 μg/mL). Plasmid DNA was isolated using the Promega Miniprep Kit (#A1222). Lentiviruses encoding sgRNAs were produced as described above. TPC-1 cells were seeded at 4 × 105

(19) An interplay of non-coding RNAs regulates CDH13 expression and affects endothelial function and coronary artery disease riskResearch SquareOctober 31, 2025Shuangyue Li, Xiaoning Song, Anastasiia Diagel, Ling Li, Aldo Moggio, Yifan Chen, Zhaolong Li, Tan Dang, Miaomiao Li, Rui Shen, et al.medium with antibiotics, and extracted using the PureYield™ Plasmid Mini/Max-prep System (Promega, A1223 /A2392). Linearization of lentiCRISPRv2 and lentiSAMv2 was performed using Esp3I (Thermo Scientific,

(20) CRISPRi-seq in Haemophilus influenzae reveals genome-wide and medium-specific growth determinantsPLOS PathogensOctober 31, 2025Celia Gil-Campillo, Johann Mignolet, Asier Domínguez-San Pedro, Beatriz Rapún-Araiz, Axel B. Janssen, Vincent de Bakker, Jan-Willem Veening, Junkal Garmendiawas selected for culturing and plasmid purification using PureYield Plasmid Miniprep System (Promega, A1222). Purified plasmids were used to amplify each sgRNA cassette by PCR with F_AarI-Hi0601up/2477 and R_AarI_Hi0601dn/2482

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(1)

3/25/2016Transfection Quality Plasmid DNA in Shortest Time Sivaraman NatarajanOur lab work is mostly focused on lineage tracing of cancer cells. We use Promega’s mini prep kit for various plasmid DNA preparations for cloning as well as transfection. This Procedure is very ... Read Review
(1)

1/20/2015Quick and pure miniprep kit for plasmid DNA CHING-SHYI WUPreparation of miniprep plasmid DNA is very common in most Bio-related labs. The purified DNA is used for cloning, DNA sequencing, PCR, and other processes. The PureYield miniprep kit from Promega is ... Read Review