PureYield Plasmid Miniprep System from Promega

Product Specs
ItemPureYield Plasmid Miniprep System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA1223
Time> 10 minutes
Volume
- If higher yields are desired-1.5–3.0ml of culture
- low-copynumber plasmids- 3ml culture volumes are recommended
Yieldpurify 1.5–7.5 µg of plasmid DNA
Quantity100 preps
Methodsilica membrane column
SourceBacterial
Nucleic Acid TypePlasmid DNA
Applicationspurification

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Agrobacterial Transformation Enhancement by Improved Competent Cell Preparation and Optimized ElectroporationLife (Basel)November 17, 2023Xiang Liu, Joseph F. Miceli, Sabrina Patton, Melissa Murray, John Evans, Xiaoping Wei, Pohao Wangwere incubated at 37 °C. Plasmid DNA was prepared using the PureYield plasmid miniprep system (Promega A1223) following the manufacturer’s instructions. The plasmid sequences were validated by NGS sequencing. Two

(2) Generation of porcine PK-15 cells lacking the Ifnar1 or Stat2 gene to optimize the efficiency of viral isolationPLOS ONENovember 8, 2023Maya Shofa, Akatsuki SaitoC2992H) and extracted using the PureYield Plasmid Miniprep System (Promega, Madison, WI, USA, Cat# A1222). The sequence of the plasmid was verified by sequencing using a SupreDye v3.1 Cycle Sequencing Kit

(3) A method for measurement of human asparagine synthetase (ASNS) activity and application to ASNS protein variants associated with ASNS deficiencyBiology Methods and ProtocolsOctober 25, 2023Mario C Chang, Stephen J Staklinski, Matthew E Merritt, Michael S KilbergT337I ASNS variants. The plasmids were purified with the PureYield™ Plasmid Miniprep System (Promega A1223), and the coding regions were confirmed by DNA sequencing. WT and variant ASNS plasmids were transfected

(4) Activation of GPR44 decreases severity of myeloid leukemia via specific targeting of leukemia initiating stem cellsCell reportsJuly 25, 2023Qian F, Nettleford SK, Zhou J, Arner BE, Hall MA, Sharma A, Annageldiyev C, Rossi RM, Tukaramrao DB, Sarkar D, et al.8802–6848-74 Plasmid Maxi Kit (25),Qiagen,Cat#:12163 PureYield™ Plasmid Miniprep System,Promega,Cat# A1223 Wizard® SV Gel and PCR Clean-Up System (50 preps),Promega,Cat# A9281 RNA 6000 Nano Kit,Agilent,Cat#

(5) Accurate profiling of full-length Fv in highly homologous antibody libraries using UMI tagged short readsNucleic Acids ResearchJune 23, 2023Itay Levin, Marek Štrajbl, Yair Fastman, Dror Baran, Shir Twito, Jessica Mioduser, Adi Keren, Sharon Fischman, Michael Zhenin, Guy Nimrod, et al.collected, and the plasmid DNA was isolated using PureYield™ Plasmid Miniprep System (Promega, Cat. No. A1222). The library DNA amount was quantified and then diluted in a series of dilution rounds in order to

(6) Localized modulation of DNA supercoiling, triggered by the Shigella anti-silencer VirB, is sufficient to relieve H-NS-mediated silencingNucleic Acids ResearchMay 8, 2023Michael A Picker, Monika M A Karney, Taylor M Gerson, Alexander D Karabachev, Juan C Duhart, Joy A McKenna, Helen J Wingthe PureYield Plasmid Miniprep System according to the manufacturer's directions (Promega, Cat. No. A1222). For in vivo supercoiling assays in Shigella, the PicsP-lacZ reporter was introduced into the wild-type

(7) Prevalence of Extended-Spectrum β-Lactamases in Multidrug-Resistant Klebsiella pneumoniae Isolates in Jordanian HospitalsJournal of Epidemiology and Global HealthApril 24, 2023Suhaila A. Al-Sheboul, Ghina S. Al-Madi, Brent Brown, Wail A. Hayajnehsample. Plasmid extraction was performed using Promega PureYieldTM Plasmid Miniprep System (Catalogue #A1223, USA) and according to the manufacturer’s instructions. After plasmid extraction, the concentration

(8) Preparation of chaperone-loaded neural stem cell-derived extracellular vesicles to reduce protein aggregation in Huntington’s disease cellular modelsSTAR ProtocolsMarch 1, 2023Bhagyashree S. Joshi, Inge S. Zuhorn,GE Healthcare,RPN2232 QIAquick PCR & Gel Cleanup Kit (100),Qiagen,28506 PureYield™ Plasmid Miniprep System,Promega,A1222 ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines ,, Human

(9) Role of human HSPE1 for OPA1 processing independent of HSPD1iScienceFebruary 17, 2023Yeung N, Murata D, Iijima M, Sesaki H.commercial assays ,, RNAeasy Mini Kit,Qiagen,Cat# 74106 Pure Yield Plasmid Mini Prep System,Promega,Cat# A1222 PowerUp SYBR Green Master Mix,Applied Biosystems,Cat# A25742 ReadyScript cDNA Synthesis Mix,Sigma-Aldrich,Cat#

(10) Do Amino Acid Antiporters Have Asymmetric Substrate Specificity?BiomoleculesFebruary 6, 2023Gregory Gauthier-Coles, Stephen J. Fairweather, Angelika Bröer, Stefan Bröerpropagated before isolating the plasmids using the Wizard Plus SV Miniprep DNA purification system (Promega; A1223). Plasmids were then sent to the Biomolecular Resource Facility (ANU) for Sanger sequencing. In vitro transcription

(11) A noncanonical RNA-binding domain of the fragile X protein, FMRP, elicits translational repression independent of mRNA G-quadruplexesJournal of Biological ChemistryDecember 1, 2022MaKenzie R. Scarpitti, Julia E. Warrick, Evelyn L. Yoder, Michael G. Kearse(Thermo Fisher # C404006), purified using the PureYield Plasmid Miniprep or Midiprep Systems (Promega # A1222 and A2495), and validated by Sanger sequencing at The Ohio State University Comprehensive Cancer Center

(12) Membrane phosphoinositides regulate GPCR-β-arrestin complex assembly and dynamicsCellNovember 23, 2022John Janetzko, Ryoji Kise, Benjamin Barsi-Rhyne, Dirk H. Siepe, Franziska M. Heydenreich, Kouki Kawakami, Matthieu Masureel, Shoji Maeda, K. Christopher Garcia, Mark von Zastrow, et al.E2621X NucleoBond Xtra Midi Plus,Macherey-Nagel,Cat# 740412.50 PureYield Plasmid Miniprep System,Promega,Cat# A1222 FastGene Plasmid Mini Kit,Nippon Genetics,Cat# FG-90502 MycoAlert Mycoplasma Detection Kit,Lonza,Cat#

(13) Genetic Modulation of the GLUT1 Transporter Expression—Potential Relevance in Complex DiseasesBiology (Basel)November 16, 2022Anna Kulin, Nóra Kucsma, Balázs Bohár, Botond Literáti-Nagy, László Korányi, Judit Cserepes, Anikó Somogyi, Balázs Sarkadi, Edit Szabó, György Váradyultra-competent cells and purified using the PureYield Plasmid Miniprep System (Promega, Madison, WI, USA, A1223). To ensure the ligation was correct, we sent the constructs for Sanger sequencing. 2.5. Cell Culture,

(14) Relating individual cell division events to single-cell ERK and Akt activity time coursesScientific ReportsOctober 27, 2022Alan D. Stern, Gregory R. Smith, Luis C. Santos, Deepraj Sarmah, Xiang Zhang, Xiaoming Lu, Federico Iuricich, Gaurav Pandey, Ravi Iyengar, Marc R. Birtwistlecontaining the Akt KTR. Transformants were miniprepped with the PureYield Plasmid Miniprep System (Promega A1223) and Sanger sequence verified using GeneWiz. Akt KTR expression vector was generated by performing a

(15) Single-Molecule Studies on the Motion and Force Generation of the Kinesin-3 Motor KIF1AOptical TweezersOctober 21, 2022Lu Rao, Arne Gennerichroom temperature. A temperature-controlled shaker. PureYield™ plasmid miniprep system (Promega, #A1223). 2.2 Mutagenesis Q5® site-directed mutagenesis Kit (New England Biolabs, #E0554S). Primers for

(16) Generation of a bovine cell line for gene engineering using an HIV-1-based lentiviral vectorScientific ReportsOctober 18, 2022Nanami Morizako, Erika P. Butlertanaka, Yuri L. Tanaka, Honoka Shibata, Tamaki Okabayashi, Hirohisa Mekata, Akatsuki Saito(NEB, Cat# C2992H). The plasmids were extracted with PureYield Plasmid Miniprep System (Promega, Cat# A1222). The sequences of bovine TRIM5 mRNA (Accession# LC727630) and human TRIM5α mRNA (Accession# LC727631)

(17) Intrachromosomal Looping and Histone K27 Methylation Coordinately Regulates the lncRNA H19-Fetal Mitogen IGF2 Imprinting Cluster in the Decidual Microenvironment of Early PregnancyCellsOctober 5, 2022Xue Wen, Qi Zhang, Lei Zhou, Zhaozhi Li, Xue Wei, Wang Yang, Jiaomei Zhang, Hui Li, Zijun Xu, Xueling Cui, et al.transformed into TOP10. Plasmid DNA was collected by Wizard® Plasmid DNA Purification kit (Promega, A1223, MO, USA) and sequenced. 4.9. Chromosome Conformation Capture (3C) Furthermore, 3C assays were performed

(18) De novo sphingolipid biosynthesis necessitates detoxification in cancer cellsCell reportsSeptember 27, 2022Spears ME, Lee N, Hwang S, Park SJ, Carlisle AE, Li R, Doshi MB, Armando AM, Gao J, Simin K, et al.Cells (Agilent #200315) and DNA was miniprepped with the PureYield Plasmid Miniprep System (Promega #A1222) for lentiviral transfection. Lentivirus production and CRISPR gene KO in cell lines Lentiviruses

(19) Transcriptomic plasticity of the hypothalamic osmoregulatory control centre of the Arabian dromedary camelCommunications BiologySeptember 23, 2022Panjiao Lin et al.harbouring the inserts of interest were harvested using PureYieldTM Plasmid Miniprep System Kit (Promega, A1222) and sequenced by Eurofins Genomics using the Sanger dideoxy sequencing method. In order to predict

(20) Protocol to study sufficiency of cis-regulatory elements in mouse embryonic stem cells using a CRISPR-mediated knockin approachSTAR ProtocolsSeptember 16, 2022Tomas Pachano, Alvaro Rada-Iglesias28506 FuGENE HD Transfection Reagent,Promega,Cat: E2312 PureYield Plasmid Miniprep System,Promega,Cat: A1222 Q5® High-Fidelity 2× Master Mix,New England Biolabs,Cat: M0492L GoTaq polymerase,Promega,Cat: M7123 PureLink™ Genomic DNA Mini Kit

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(1)

3/25/2016Transfection Quality Plasmid DNA in Shortest Time Sivaraman NatarajanOur lab work is mostly focused on lineage tracing of cancer cells. We use Promega’s mini prep kit for various plasmid DNA preparations for cloning as well as transfection. This Procedure is very ... Read Review
(1)

1/20/2015Quick and pure miniprep kit for plasmid DNA CHING-SHYI WUPreparation of miniprep plasmid DNA is very common in most Bio-related labs. The purified DNA is used for cloning, DNA sequencing, PCR, and other processes. The PureYield miniprep kit from Promega is ... Read Review