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Plasmid Purification Kits (Mini)
PureYield Plasmid Miniprep System
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PureYield Plasmid Miniprep System
PureYield Plasmid Miniprep System
PureYield Plasmid Miniprep System from Promega
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Citations:
(68)
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PureYield Plasmid Miniprep System
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Description
The PureYield Plasmid Miniprep System is designed to rapidly isolate highly pure plasmid DNA. Yields up to 15ug of plasmid DNA from 600ul to 3ml of bacterial culture
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
PureYield Plasmid Miniprep System
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
A1223
Time
> 10 minutes
Volume
If higher yields are desired-1.5–3.0ml of culture
low-copynumber plasmids- 3ml culture volumes are recommended
Yield
purify 1.5–7.5 µg of plasmid DNA
Quantity
100 preps
Method
silica membrane column
Source
Bacterial
Nucleic Acid Type
Plasmid DNA
Applications
purification
Add to Compare List
Promega
2800 Woods Hollow Rd.
Madison
,
Wisconsin
United States
Phone
: 800-356-9526
Fax: 800-356-1970
Company Profile
Website:
http://www.promega.com
Citations (68)
(41)
Three-dimensional growth of breast cancer cells potentiates the anti-tumor effects of unacylated ghrelin and AZP-531
eLife
July 15, 2020
Au CC, Furness JB, Britt K, Oshchepkova S, Ladumor H, Soo KY, Callaghan B, Gerard C, Inghirami G, Mittal V,
et al
.
,
Premo FUCCI Cell Cycle Sensor (BacMam 2.0
),
ThermoFisher Scientific
,Cat#
P36237
, Commercial
assay
or kit,
PureYield Plasmid Miniprep System
,
Promega
,Cat#
A1223
, Commercial
assay
or kit,
PureYield Plasmid Maxiprep System
,
Promega
,Cat#
A2392
, "Chemical compound,
(42)
Schistosoma mansoni FES Tyrosine Kinase Involvement in the Mammalian Schistosomiasis Outcome and Miracidia Infection Capability in Biomphalaria glabrata
Frontiers in Microbiology
June 11, 2020
Naiara Clemente Tavares, Sandra Grossi Gava, Gabriella Parreiras Torres, Clara Ênia Soares de Paiva, Bernardo Pereira Moreira, Felipe Miguel Nery Lunkes, Langia Colli Montresor, Roberta Lima Caldeira, Marina Moraes Mourão
Wizard SV Gel and PCR Clean-Up System
(
A9282
), pGEM-T Easy vector (
A1360
),
PureYield Plasmid Miniprep System
(
A1222
),
T7 RiboMAX Express RNAi System
(
P1700
),
SV Total RNA Isolation System
(Z3105), and ImProm-II Reverse
(43)
Synthesis of Quenchbodies for One-Pot Detection of Stimulant Drug Methamphetamine
Methods and Protocols
June 11, 2020
Hee-Jin Jeong, Jinhua Dong, Chang-Hun Yeom, Hiroshi Ueda
chloride (Wako, Osaka, Japan; Cat. No.: 160-03555)Plasmid Miniprep kit (
Promega
, Tokyo, Japan; Cat. No.:
A1223
)SHuffle T7 Express LysY Competent Cells (New England Biolabs, Tokyo, Japan; Cat. No.: C3030)Isopropyl
(44)
mRNA stem-loops can pause the ribosome by hindering A-site tRNA binding
eLife
May 19, 2020
Chen Bao, Sarah Loerch, Clarence Ling, Andrei A Korostelev, Nikolaus Grigorieff, Dmitri N Ermolenko
polymerase,doi: 10.1073/pnas.95.2.515,, Commercial
assay
or kit,
Plasmid Miniprep
System,
Promega
,Catalog #:
A1223
, Commercial
assay
or kit,Gel and
PCR Clean-Up
System,
Promega
,Catalog #:
A9281
, Commercial
assay
or
(45)
Comparative characterization of flavivirus production in two cell lines: Human hepatoma-derived Huh7.5.1-8 and African green monkey kidney-derived Vero
PLOS ONE
April 24, 2020
Kyoko Saito, Masayoshi Fukasawa, Yoshitaka Shirasago, Ryosuke Suzuki, Naoki Osada, Toshiyuki Yamaji, Takaji Wakita, Eiji Konishi, Kentaro Hanada
constructs were purified by the
PureYield™ Plasmid Miniprep System
(
Promega
, Madison, WI, USA; No.
A1222
), and their sequences were verified by
Sanger sequencing
. PLOS ONE | https://doi.org/10.1371/journal.pone.0232274
(46)
Potent neutralizing monoclonal antibodies against Ebola virus isolated from vaccinated donors
mAbs
March 28, 2020
Pengfei Fan et al.
Vector NTI (Thermo Fisher Scientific) and extracted using the PureYieldTM
plasmid miniprep
system (
A1222
;
Promega
). Heavy and light chains (15 μg each) were used for expression in Expi293 F™ cells as described
(47)
Fetal membrane bacterial load is increased in histologically confirmed inflammatory chorioamnionitis: A retrospective cohort study
Placenta
February 1, 2020
Rochelle Hockney, Gareth J. Waring, Gillian Taylor, Stephen P. Cummings, Stephen C. Robson, Caroline H. Orr, Andrew Nelson
System (
Promega
, Cat No:
A1360
). Plasmids were isolated using PureYield
Plasmid MiniPrep
(
Promega
, Cat No:
A1223
). A ten-fold serial dilution of pooled isolated plasmids was performed to create standard curves. Absolute
(48)
Characterization of Human Mucosal‐associated Invariant T (MAIT) Cells
Current Protocols in Immunology
December 1, 2019
Michael N.T. Souter et al.
for small scale plasmid preparation, such as the
PureYield Plasmid Miniprep System
(
Promega
, cat. no.
A1223
) Reagents, equipment and their use for counting viable cells using trypan blue exclusion (see Appendix) PBS
(49)
The Mars1 kinase confers photoprotection through signaling in the chloroplast unfolded protein response
eLife
October 15, 2019
Karina Perlaza, Hannah Toutkoushian, Morgane Boone, Mable Lam, Masakazu Iwai, Martin C Jonikas, Peter Walter, Silvia Ramundo
Polymerase,
ThermoFisher Scientific
,
F530L
, Commercial
assay
or kit,
PureYield Plasmid Miniprep System
,
Promega
,
A1222
, Commercial
assay
or kit,NucleoSpin Gel and PCR Clean-up,Clontech,740609, Commercial
assay
or kit,
Direct-zol RNA Miniprep Plus
(50)
Intracellular cholesterol trafficking is dependent upon NPC2 interaction with lysobisphosphatidic acid
eLife
October 3, 2019
Leslie A McCauliff, Annette Langan, Ran Li, Olga Ilnytska, Debosreeta Bose, Miriam Waghalter, Kimberly Lai, Peter C Kahn, Judith Storch
Mutagenesis Kit,
Agilent
,Cat#
200523
, Commercial
assay
or kit,
PureYield Plasmid Miniprep System
,
Promega
,Cat#
A1223
, "Software, algorithm",Orientation of Proteins in
Membranes
(OPM),PMID: 16397007,http://opm.phar.umich.edu/
(51)
Dual and selective inhibitors of pteridine reductase 1 (PTR1) and dihydrofolate reductase-thymidylate synthase (DHFR-TS) from
Leishmania chagasi
Journal of Enzyme Inhibition and Medicinal Chemistry
August 15, 2019
Bárbara Velame Ferreira Teixeira, André Lacerda Braga Teles, Suellen Gonçalves da da Silva, Camila Carane Bitencourt Brito, Humberto Fonseca de de Freitas, Acássia Benjamim Leal Pires, Thamires Quadros Froes, Marcelo Santos Castilho
their expression-plasmid DNA extracted with the
aid
of
Pureyield Plasmid Miniprep System
kit (
Promega
–
A1223
). The extracted plasmid was used for heat
shock
transformation28 of the chemically competent E. coli
(52)
Illuminating G-Protein-Coupling Selectivity of GPCRs
Cell
June 13, 2019
Inoue A, Raimondi F, Kadji FMN, Singh G, Kishi T, Uwamizu A, Ono Y, Shinjo Y, Ishida S, Arang N,
et al
.
,
New England BioLabs
,
E2621X
NucleoBond Xtra Midi Plus
,
Macherey-Nagel
,740412.50
PureYield Plasmid Miniprep System
,
Promega
,
A1222
MycoAlert Mycoplasma Detection Kit
,
Lonza
,
LT07–118
High-Capacity cDNA Reverse Transcription Kits
,
Thermo Fisher Scientific
(53)
The Arabian camel, Camelus dromedarius interferon epsilon: Functional expression, in vitro refolding, purification and cytotoxicity on breast cancer cell lines
PloS one
January 1, 2019
Abdel-Fattah M, Saeed H, El-Shennawy L, Shalaby M, Embaby A, Ataya F, Mahmoud H, Hussein A.
plasmids were prepared from some positive
clones
using the
PureYield Plasmid Miniprep System
(Cat #
A1222
,
Promega
, Madison, USA). The
sequencing
of the cloned insert was carried out according to Sanger et
(54)
Efficient In Vivo Liver-Directed Gene Editing Using CRISPR/Cas9
Molecular Therapy
May 1, 2018
Kshitiz Singh, Hanneke Evens, Nisha Nair, Melvin Y. Rincón, Shilpita Sarcar, Ermira Samara-Kuko, Marinee K. Chuah, Thierry VandenDriessche
(K2875-J10; Life Technologies). The plasmid DNAs were isolated using pureyield
plasmid miniprep
system (
A1222
;
Promega
). Plasmids were sequenced using an M13 forward primer (5′-GTAAAACGACGGCCAG-3′) by the
Sanger sequencing
(55)
Restricted HIV-1 Env glycan engagement by lectin-reengineered DAVEI protein chimera is sufficient for lytic inactivation of the virus
Biochemical Journal
March 15, 2018
Bibek Parajuli, Kriti Acharya, Harry C. Bach, Bijay Parajuli, Shiyu Zhang, Amos B. Smith, Cameron F. Abrams, Irwin Chaiken
were purchased from
ATCC
. The
DNA purification
was conducted using the
Promega
Miniprep Kit (Cat. No.
A1222
). BamH1 (Cat. No.
R0136S
) and Nde1 (
R0111S
) restriction enzymes and DNA ligase (Cat. No.
M0202S
) were
(56)
CRISPR-based herd immunity can limit phage epidemics in bacterial populations
eLife
March 9, 2018
Pavel Payne, Lukas Geyrhofer, Nicholas H Barton, Jonathan P Bollback
paper,NA,Plasmid derived from pCas9 commercial
assay
or kit,
PureYield Plasmid Miniprep System
,
Promega
,
Promega
:
A1223
; RRID:SCR_006724, "chemical compound, drug",
Chloramphenicol
,
Sigma-Aldrich
,Sigma-Aldrich:C0378-5G; RRID:SCR_008988, "software,
(57)
RORα controls inflammatory state of human macrophages
PloS one
January 1, 2018
Nejati Moharrami N, Bjørkøy Tande E, Ryan L, Espevik T, Boyartchuk V.
T4 DNA ligase reaction buffer
(B0202) were from
New England Biolabs
.
PureYield Plasmid Miniprep System
(
A1222
) was from
Promega
. PLOS ONE | https://doi.org/10.1371/journal.pone.0207374 November 28, 2018 2 / 15 RORA
(58)
Transcription Factor Forkhead Regulates Expression of Antimicrobial Peptides in the Tobacco Hornworm, Manduca sexta
Scientific Reports
December 1, 2017
Xue Zhong, Munmun Chowdhury, Chun-Feng Li, Xiao-Qiang Yu
vectors (
A1360
,
Promega
). Recombinant T-vectors were purified using
PureYield™ Plasmid Miniprep System
(
A1222
,
Promega
) according to the manufacturer’s instruction and digested with Kpn I/Not I restriction enzymes,
(59)
Teneurin-2 presence in rat and human odontoblasts
PLOS ONE
September 19, 2017
K. R. Torres-da-Silva, G. W. L. Tessarin, C. A. Dias, I. Z. Guiati, E. Ervolino, A. Gonçalves, I. M. Beneti, D. A. Lovejoy, C. A. Casatti
with positive
clones
were selected using blue-white colony
screening
and the plasmids were purified (
A1223
,
PureYield Plasmid Miniprep System
, WI, USA). Plasmids were submitted to restriction digestion to confirm
(60)
Decoupling of DNA methylation and activity of intergenic LINE-1 promoters in colorectal cancer
Epigenetics
June 3, 2017
Natasha Vafadar-Isfahani et al.
Spin miniprep kit (
QIAGEN
, Cat. no.
27106
) or
PureYield™ Plasmid Miniprep System
(
Promega
, Cat. no.
A1223
). Plasmid DNA was sent to Source Biosciences for
Sanger sequencing
.
Sequencing
data was analyzed using
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Reviews
Write a Review
(1)
3/25/2016
Transfection Quality Plasmid DNA in Shortest Time
Sivaraman Natarajan
Our lab work is mostly focused on lineage tracing of cancer cells. We use Promega’s mini prep kit for various plasmid DNA preparations for cloning as well as transfection. This Procedure is very ...
Read Review
(1)
1/20/2015
Quick and pure miniprep kit for plasmid DNA
CHING-SHYI WU
Preparation of miniprep plasmid DNA is very common in most Bio-related labs. The purified DNA is used for cloning, DNA sequencing, PCR, and other processes. The PureYield miniprep kit from Promega is ...
Read Review
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PureYield Plasmid Miniprep System from Promega
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