ZAN CHEN

Postdoctoral Researcher

JOHNS HOPKINS UNIVERSITY School of Medicine

Kit for Quick Titration of Baculovirus
July 26, 2021
We use this kit to measure the titer of baculovirus for insect cell expression. It works well but requires a lot of labor read more
Crystal Screen Kit with Wide Range of Conditions
July 26, 2021
I was able to get protein crystals with Morpheus. However, it was very difficult to optimize and reproduce the condition as it ... read more
Plasmid Construction
July 25, 2021
We use Genscript to do a lot of cloning to make new plasmid and modifications/subcloning. The service is usually good. Sometimes ... read more
Buffer Exchange for Desalting
July 25, 2021
These columns from Biorad are for buffer exchange or desalting of protein samples. It worked very well for me. read more
Gel Extraction Kit for Molecular Cloning
July 23, 2021
I use this kit to do gel extraction when doing cloning for various projects. I was able to get purified DNA fragment and ... read more
Desalting and Concentration of Plasmid DNA
July 23, 2021
I use this kit to desalt and concentrate plasmid DNA and it works very well with high recovery of the original sample. read more
Miniprep Kit Works Really Well
July 20, 2021
This miniprep kit from Thermo works as well as the one from Qiagen. read more
Colume to Purify Antibodies Gives Great Results
July 20, 2021
We use this column to purify IgG antibodies. It works very well to bind specifically to antibodies and elute with lower pH. read more
Fast and Easy to Use to Conjugate Streptavidin to Antibody
June 04, 2021
I used this kit to conjugate streptavidin to antibody. The protocol is really simple. One thing I don't like about this kit is ... read more
Buffer Exchange or Desalting for Protein Sample
May 31, 2021
This is a tiny gel filtration column that enables buffer exchange/desalting of protein sample. It works well, but has some ... read more

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Robust Immunostaining of Iba1 with a Reliable Antibody

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We utilize the Iba1 antibody to measure Iba1 expression in mouse brain tissue to detect microglial activation. Iba1 (ionized calcium-binding adaptor molecule 1;... read more

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