Buffer Exchange or Desalting for Protein Sample

JOHNS HOPKINS UNIVERSITY School of Medicine
Department of Biophysics
Postdoctoral Researcher

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Quality of Results

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Company:

Thermo Scientific

Product Name:

Zeba™ Spin Desalting Columns, 7K MWCO, 2 mL

Catalog Number:

89889

This is a tiny gel filtration column that enables buffer exchange/desalting of protein sample. It works well, but has some limitations

Experimental Design and Results Summary

Application

Buffer exchange or desalting of protein sample

Starting Material

Protein sample

Tips

Follow the protocol carefully

Results Summary

The buffer exchange or desalting works well. But I lose about 30-40% of the sample, and the volume of the sample after the process increases.

DOI or PMID #

N/A

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Summary

The Good

Works well for the desalting purpose

The Bad

low recovery of sample

The Bottom Line

These columns work well to desalt/buffer exchange protein samples, though the recovery is not great and the volume of sample would change dramatically.

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