Jeffrey Fites

Postdoc

UW-Madison

Assay Kit/Reagent User Review
Comparison of Mirus TransIT Transfection Reagents for RNA Silencing
October 11, 2019
Both Mirus transfection reagents work for RNAi gene silencing, but as expected the specific reagent for RNAi, TransIT-TKO, worked ... read more
Antibody User Review
Anti-IL-4 Receptor, BV421
February 04, 2019
This product works. Probably insufficient for use in gating populations, but with appropriate controls sufficient for ... read more
Antibody User Review
Anti-TNF Receptor 2 Antibody, BV421
December 07, 2018
CD120b is a receptor for TNF alpha and is also known as TNF receptor 2 (TNFR2). To track expression of TNFR2 on neutrophils, I ... read more
Antibody User Review
Staining Neutrophils With Anti-Mouse GM-CSF R Alpha
September 25, 2018
I stained neutrophils for GM-CSF receptor alpha. These neutrophils were differentiated from murine granulocyte-macrophage ... read more
Assay Kit/Reagent User Review
Preparing Single Stain Compensation Controls for Flow Cytometry
June 27, 2018
UltraComp eBeads are an easy way to prepare single stain compensation controls to set up a flow cytometry experiment. ThermoFisher... read more
Antibody User Review
APC Anti-CD115 Antibody
March 06, 2018
I use anti-CD115 (M-CSF receptor) to aid in labeling monocyte lineage cells. I titrated the APC-conjugated antibody on bone marrow... read more
Antibody User Review
Staining Bone Marrow Progenitors With Anti-CD117 (C-Kit) PE-Cy7
November 16, 2017
In order to track differentiation of myeloid cells from progenitors, I used CD117 (c-kit) to marker the cells in a progenitor ... read more
Antibody User Review
Staining Mouse Antigen Presenting Cells With PacBlue Anti-CD40
September 27, 2017
CD40 is a costimulatory ligand found on antigen presenting cells (B cells, dendritic cells, ect.). I wanted to track CD40 ... read more
Antibody User Review
Staining Murine Leukocytes With Anti-TLR4 Clone SA15-21
August 10, 2017
There are two main commercial clones that stain for TLR4 (CD284). MTS510 recognizes the TLR-4/M2 complex (together these bind LPS)... read more
Antibody User Review
Staining Mouse Antigen Presenting Cells With PE Anti-Mac-2
June 07, 2017
Mac2, also known as Galectin-3, is a pattern recognition receptor on various leukocyte populations. I titrated the anti-mouse Mac2... read more
Antibody User Review
Anti-murine F4/80 FITC
May 01, 2017
F4/80 is a marker found on macrophages. For purposes of staining macrophages in multiple tissues, I titrated anti-F4/80-FITC on ... read more
Antibody User Review
Pac Blue Anti-Siglec H staining for pDCs
April 12, 2017
Plasmacytoidal dendritic cells (pDCs) are characterized in mice as expressing CD11c (on most DCs) and the B cell marker B220. A ... read more
Antibody User Review
Positive Enrichment Of CD4 T Cells From Mouse
February 27, 2017
I used these anti-CD4 coated beads to enrich CD4 T cells from mouse spleen and lymph nodes for in vitro applications. read more
Antibody User Review
Anti-murine CXCR2 FITC
December 15, 2016
I am interested in expression of CXCR2, which is expressed on neutrophils and is important for neutrophil migration. I am ... read more
Antibody User Review
PE anti-Dectin-1 antibody
November 15, 2016
Dectin-1 is a pathogen recognition receptor important for recognition of fungi. I had purchased this antibody to look at Dectin-1 ... read more

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Efficient and Cost Friendly SYBR Green Mastermix for qPCR

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I study the transcription of endogenous genes regulated by transcription factors, and it's crucial to quantify their expression levels accurately. To achieve ... read more

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