BioLegend
PE anti-mouse/human Mac-2 (Galectin-3) Antibody
125405
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Mac2, also known as Galectin-3, is a pattern recognition receptor on various leukocyte populations. I titrated the anti-mouse Mac2 antibody on cells from the spleen or lymph nodes from naïve mice. Resident DCs (MHC class II+, CD11c+, CD11b-, F4/80-) in these lymphatic tissues had one of the largest proportion of Mac2+ cells in naïve mice. I titrated the antibody in the concentration range suggested by BioLegend finding that 1:200 dilution of the antibody stained better than 1:100 and 1:50.
Flow Cytometry
Splenocytes or cells from skin-draining lymph nodes from naive C57B/6 mice
Titrations (as shown) for 30 minutes at 4C along with antibodies against other markers for gating.
Buffer contained BSA and FcBlock
Cells were fixed with 2% paraformaldehyde
None
Flow cytometry
I show gated DCs from the spleen and pooled skin-draining LNs from naïve C57B/6 mice (MHC class II+, CD11c+, CD11b-, F4/80-). The same sample data is shown after staining with different concentrations of anti-Mac2-PE. Around 5% of these DCs were positive for Mac-2. While the staining profile was not incredibly different among the titrations, the 1:200 dilution was the best.
N/A
I have been using this product at 1:200 on antigen-presenting cells since this titration experiment with good data.
Stains well
This product works well. I have been using it assays with success.