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Author Profile
Jason Spaeth
Postdoctoral Research Fellow
Vanderbilt University Medical Center
Articles by Jason Spaeth
Antibody User Review
Brg1 Staining In Mouse Pancreatic Cryosection
August 24, 2017
Our goal is to evaluate the contribution the Brg1 ATPase subunit of the Swi/Snf chromatin remodeling complex has in pancreatic ...
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Antibody User Review
Brm Staining In Pancreatic Cryosection
August 23, 2017
Our focus is on the influence Brm, an ATPase subunit of the Swi/Snf chromatin remodeling complex, has in pancreatic islets. ...
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Antibody User Review
Urocortin III In Mouse Cryosections
August 22, 2017
Urocortin III is present in the mature beta cells of pancreatic islets. Staining of 8 week old mouse cryosections revealed near ...
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Antibody User Review
ALDH1A3 Staining In Dedifferentiated Pancreas Tissue
March 09, 2017
Our lab focused on loss of beta cell identity. We aimed to determine whether the dedifferentiation marker Aldh1a3 is expressed in...
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Antibody User Review
SLC18A2 Staining in Human Pancreas Tissue
May 09, 2016
We are optimizing staining conditions of SLC18A2 in human cryoembedded tissue. This antibody works best at 1:100 dilution.
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Antibody User Review
Pax6 Staining in Pancreatic Cryosection
March 08, 2016
The Pax6 transcription factor is a pan-endocrine marker in the islets of langerhans. We were optimizing staining conditions in ...
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Antibody User Review
Glut2 Staining in Pancreatic Cryosection
March 07, 2016
Glut2 is a low-affinity glucose transporter present in pancreatic beta cells, hepatocytes and basolateral membranes of intestinal ...
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Antibody User Review
IP/Western of NFAT from Mouse beta Cell Line
March 01, 2016
NFATc proteins (NFATc1–4) are expressed in ß-cells as well as in other hormone-producing islet cells. We aimed to optimize ...
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Antibody User Review
MafA Staining in Pancreatic Cryosections
February 25, 2016
MafA is an islet enriched transcription factor that is present in ~85% of all islet beta cells. It is specifically expressed in ...
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Antibody User Review
GP2 Antibody Staining in Human Pancreas
February 23, 2016
We aimed to optimize staining of human pancreas tissue with GP2 antibody. This antibody worked best at 1:100 and showed strong ...
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Antibody User Review
MIP-CreERT2 Pancreatic Cryosection 1 Day after Tamoxifen Treatment
February 18, 2016
Cre transgenes are driven by specific gene regulatory sequences, in our case, under the Mouse Insulin Promoter. The Cre-ERT2 is ...
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Antibody User Review
Immunofluorescence of Glucagon and Insulin in Pancreatic Mouse Cryosection
February 05, 2016
We aimed to optimize Glucagon staining in our pancreatic mouse sections using a Rabbit Glucagon antibody. Novus produces ...
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Antibody User Review
Immunofluorescence of Dnmt3a and Insulin in Mouse P3 Pancreatic Paraffin Section
February 03, 2016
We were focused on optimizing staining conditions for the Dnmt3A antibody to examine if it was re-expressed in adult pancreatic ...
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Antibody User Review
Immunofluorescence of Ki-67 and Insulin in Paraffin Embedded Mouse Tissue
January 13, 2016
We were determining the proliferative capacity of beta cells in a transgenic mouse model utilized in our lab. Ki-67 is a nuclear ...
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Antibody User Review
Sox9 Staining in e15.5 Mouse Pancreatic Tissue
January 11, 2016
The SRY/HMG box (Sox) family of transcription factors are expressed in early pancreatic development. Among these, Sox9 is ...
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Efficient and Cost Friendly SYBR Green Mastermix for qPCR
Parijat Sarkar
I study the transcription of endogenous genes regulated by transcription factors, and it's crucial to quantify their expression levels accurately. To achieve ...
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