GP2 Antibody Staining in Human Pancreas

February 23, 2016

Molecular Physiology and Biophysics
Vanderbilt University Medical Center
Postdoctoral Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus Biologicals

Product Name:

GP2 Antibody

Catalog Number:

NBP1-86061

We aimed to optimize staining of human pancreas tissue with GP2 antibody. This antibody worked best at 1:100 and showed strong cytoplasmic staining in exocrine tissue.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human pancreas tissue

Primary Incubation

1:100, Overnight, 4 Deg Celcius

Blocking Agent

5% Normal Donkey Serum in 1%BSA/PBS

Secondary Incubation

1:1000, Donkey anti-Rabbit Cy3

Tertiary Incubation

N/A

Detection

Immunofluorescence

Results Summary

This antibody worked best at 1:100 dilution. This is fairly high concentration to use this antibody regularly.

Additional Notes

None

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Summary

The Good

Good detection

The Bad

Need 1:100 dilution to achieve optimal staining

The Bottom Line

Antibody works well at 1:100

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