EMD Millipore
Anti-Cre Antibody
69050
Browse Similar Products: Cre Immunofluorescence Antibodies
Cre transgenes are driven by specific gene regulatory sequences, in our case, under the Mouse Insulin Promoter. The Cre-ERT2 is only translocated to the nucleus after following treatment with Tamoxifen. In this case, we gave transgenic mice 3 doses of Tamoxifen by oral gavage 3 times over 5 days. The pancreas was harvested, fixed in 4%PFA and embedded in OCT the day after the last Tamoxifen treatment.
Immunofluorescence
Mouse pancreas cryosection
1:500, Overnight, 4 Deg Celcius
5% Normal Donkey Serum in 1%BSA/PBS
1:1000, Donkey anti-Rabbit Cy3
N/A
The Cre antibody worked very well at 1:500 dilution, and as expected, was found to be in the nucleus of Insulin+ cells.
None
Excellent detection at 1:500
Excellent detection at 1:500 in cryosections.