Seemin Ahmed

Postdoctoral Fellow

Mass General Hospital

Fastest Western Blot I Have Ever Done
February 25, 2016
I wanted to try out a system that would reduce the time and effort put into performing western blots (and I do a lot of them). I ... read more
Differentiation of Stem Cells
November 30, 2015
I wanted to use BMP4 as a positive control for my stem cells since I was trying to identify new compounds that can induce ... read more
Detection of HIF1 Alpha
October 27, 2015
I wanted to test the efficacy of HIF 1 alpha inhibitors in cells. I artificially induced upregulation of HIF 1 alpha using Cobalt ... read more
Use of Evans Blue to monitor Blood Brain Barrier Permeability
June 29, 2015
We wanted to analyse blood brain barrier permeability in a neurologically diseased mouse model. There are many ways to analyse ... read more
Anti-Aspartoacylase Antibody (for Western blot) from Abcam
December 16, 2014
This antibody was used in a preclinical gene therapy study that investigated the feasibility of single intravenous injections to ... read more

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I used Thermo Scientific RNase A to degrade unprotected mRNA during a gel retention assay to evaluate the protective effect of lipid nanoparticles (LNPs). Using... read more

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