Fastest Western Blot I Have Ever Done

Mass General Hospital
Department of Neurosurgery
Postdoctoral Fellow

Overall

Performance

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

iBind Western Device

Catalog Number:

SLF1000

I wanted to try out a system that would reduce the time and effort put into performing western blots (and I do a lot of them). I wanted to compare the performance and efficiency of the iBind system with the traditional western blotting procedures. The primary reason behind choosing this system was that it was handsfree (leaving you more time to finish other stuff) and really fast (compared to overnight primary incubation and long washes).

Experimental Design and Results Summary

Tips

Very important to let the system sit for at least 2.5 hours. The membrane has to be placed on the card without bubbles. If you are impatient (like me) you can raise the flap on the window on the surface of the device to check the levels of buffer. the wells need to be empty before you open the casette.

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Summary

The Good

Really fast and handsfree system

The Bad

Only one antibody can be probed at a time

The Bottom Line

I loved the system and its efficiency.

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