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Author Profile
Giulia Zaffaroni
Houston Methodist
Articles by Giulia Zaffaroni
Sensitive and Reproducible RNA Quantification with a Straightforward Fluorescence-Based Workflow
August 26, 2026
We use the Quant-iT RiboGreen RNA Assay when we need greater sensitivity than absorbance-based measurements, particularly for ...
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Straightforward Antibody Labeling with Bright DyLight 488 Fluorescence
August 26, 2026
Our laboratory uses fluorescently labeled antibodies for flow cytometry and fluorescence-based cellular assays.
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Reliable On-Column DNase Treatment for Cleaner RNA and More Trustworthy Downstream Gene-Expression Data
August 26, 2026
Our laboratory performs RNA extraction and downstream gene-expression analyses from tissue samples. Removing residual genomic DNA ...
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Simple and Dependable ECL Substrate for Routine Western Blot Detection
August 26, 2026
Our laboratory uses Western blotting to evaluate changes in protein expression and signaling pathways in plasma. We routinely ...
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The Cheapest Insurance You Can Buy Against Months of Uninterpretable Data
August 19, 2026
Every cell-based experiment in our lab assumes the cells are what we think they are. Mycoplasma contamination is invisible by eye,...
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Turns Fluo-4 into a Ratiometric Measurement on a Single 488 nm Laser
August 19, 2026
Quantitative calcium flux in primary immune cells, where cell-to-cell variability in dye loading would otherwise be ...
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Viability and Cell Tracking in a Red Channel that Stays Out of the Calcium Indicators' Way
August 19, 2026
Confirming that cells are alive and esterase-active during a functional experiment is a basic requirement, and doing it in a spare...
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The Default Single-Wavelength Calcium Indicator
August 19, 2026
We measure intracellular calcium flux as the functional readout for receptor and mechanosensitive channel activation in primary ...
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The Maximum-Response Control that Makes Every Calcium Experiment Interpretable
August 18, 2026
Every calcium flux experiment we run needs a positive control that produces a maximal, receptor-independent calcium response. ...
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Excellent for Low-Concentration Protein Samples
August 13, 2026
Is a very reliable and sensitive assay for measuring low protein concentrations. I find it especially useful for exosome protein ...
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Simple and Reliable Extracellular Vesicle Isolation
August 13, 2026
Extremely easy to use and make extracellular vesicle isolation very straightforward. The workflow requires minimal hands-on effort...
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Plate Seals: Simple and Effective When Applied Correctly
August 10, 2026
Straightforward and easy to apply, making them convenient for routine qPCR experiments. When properly sealed, they provide a ...
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Reliable and Convenient Centrifugation for Routine Workflows
August 10, 2026
Routine laboratory centrifugation for cell pelleting, sample preparation, washing steps, and separation of biological samples.
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Fraction Collection Made Incredibly Simple
August 07, 2026
The Izon Automatic Fraction Collector is exceptionally easy to use and makes fraction collection almost effortless. The system is ...
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Reliable Sterilization in a Compact Format
August 07, 2026
The Tuttnauer 1730M Valueklave is a reliable and straightforward tabletop autoclave that performs well for routine laboratory ...
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Product Review
2X PCR Master Mix
Mitesh Dwivedi
The aim of the experiment was to genotype HLAB27 by PCR SSP method. Genomic DNA was subjected to PCR using 2x PCR Master Mix.
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