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Author Profile
Giulia Zaffaroni
Houston Methodist
Articles by Giulia Zaffaroni
cDNA Synthesis for RT-qPCR and Gene-Expression Analysis
September 18, 2026
I have used the Bio-Rad iScript cDNA Synthesis Kit for converting total RNA to cDNA prior to downstream gene-expression analysis ...
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Simple and Reliable DNA Recovery from Agarose Gels
September 18, 2026
I have used the PureLink Quick Gel Extraction Kit after agarose gel electrophoresis to recover DNA bands for downstream molecular ...
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Convenient Spin-Column Purification of GST-Tagged Proteins
September 18, 2026
I have used this kit for purification of GST-tagged proteins. Used for affinity purification of GST-tagged recombinant proteins ...
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Reliable Agarose for Routine DNA Gel Electrophoresis
September 18, 2026
I have used this agarose for preparing gels for routine DNA electrophoresis. The agarose is weighed according to the desired gel ...
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Effective and Easy-to-Prepare Reagent for Cell Permeabilization
September 18, 2026
Cell membrane permeabilization for intracellular staining and related cellular assays. I have used saponin as a permeabilization ...
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Preparation of Phosphate-Containing Buffers and Mobile Phases for HPLC
September 18, 2026
I have used ammonium dihydrogen phosphate for preparing aqueous HPLC buffers. The appropriate amount is weighed and dissolved in ...
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Reliable Dopamine Hydrochloride that Dissolves Easily and Gives Reproducible Results
September 14, 2026
We use dopamine hydrochloride in our biomaterials and cell-based research. For our applications, consistency of the starting ...
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Sensitive and Reproducible RNA Quantification with a Straightforward Fluorescence-Based Workflow
August 26, 2026
We use the Quant-iT RiboGreen RNA Assay when we need greater sensitivity than absorbance-based measurements, particularly for ...
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Straightforward Antibody Labeling with Bright DyLight 488 Fluorescence
August 26, 2026
Our laboratory uses fluorescently labeled antibodies for flow cytometry and fluorescence-based cellular assays.
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Reliable On-Column DNase Treatment for Cleaner RNA and More Trustworthy Downstream Gene-Expression Data
August 26, 2026
Our laboratory performs RNA extraction and downstream gene-expression analyses from tissue samples. Removing residual genomic DNA ...
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Simple and Dependable ECL Substrate for Routine Western Blot Detection
August 26, 2026
Our laboratory uses Western blotting to evaluate changes in protein expression and signaling pathways in plasma. We routinely ...
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The Cheapest Insurance You Can Buy Against Months of Uninterpretable Data
August 19, 2026
Every cell-based experiment in our lab assumes the cells are what we think they are. Mycoplasma contamination is invisible by eye,...
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Turns Fluo-4 into a Ratiometric Measurement on a Single 488 nm Laser
August 19, 2026
Quantitative calcium flux in primary immune cells, where cell-to-cell variability in dye loading would otherwise be ...
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Viability and Cell Tracking in a Red Channel that Stays Out of the Calcium Indicators' Way
August 19, 2026
Confirming that cells are alive and esterase-active during a functional experiment is a basic requirement, and doing it in a spare...
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The Default Single-Wavelength Calcium Indicator
August 19, 2026
We measure intracellular calcium flux as the functional readout for receptor and mechanosensitive channel activation in primary ...
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Strong T Cell Staining in Mouse Colonic Cell Suspensions Using Alexa 700 anti-...
The Alexa 700 anti-mouse CD90.2 antibody (clone 30-H12, BioLegend) provides a quite strong and reliable staining of T cells in mouse colonic intestinal cell ...
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