The Good
Excellent sensitivity, broad linear range, supplied RNA standards, straightforward plate-based workflow, and much more reliable than absorbance measurements for low-concentration RNA samples.
The Bad
Requires preparation of a standard curve and a fluorescence plate reader, so it is slightly more hands-on than a direct NanoDrop measurement.
The Bottom Line
A reliable and sensitive RNA quantification assay that is particularly valuable when RNA is limited or when absorbance-based measurements are approaching their lower sensitivity limit. The combination of good linearity, supplied standards, and simple workflow makes it easy to use routinely.