ID7000™ Spectral Cell Analyzer from Sony Corporation

Product Specs
ItemID7000™ Spectral Cell Analyzer
CompanySony Corporation
Price Inquire
Catalog NumberID7000
Detection32-channels (PMT arrays); can be configured with 186 detectors to detect 44 colors or more
Throughput40,000 events per second
Lasers3 to 7 lasers
Excitation320 nm, 355 nm, 405 nm, 488 nm, 561 nm, 637 nm, 808 nm
Parameters186 fluorescence channels
CapacityTubes (5 mL), Microplates using autosampler (96-well, 384-well)
Data Throughput40,000 events/sec
Dimensions(WxDxH) 106 x 71.9 x 76 cm
Flow Rate33 to 200 µL/min

(161) Structure of the Gq-coupled adhesion receptor ADGRL4Nature CommunicationsDecember 30, 2025Qingchao Chen, Anastasiia Gusach, Aurora Diamante, Jayesh C. Patel, Patricia C. Edwards, Christopher G. Tate, David M. Favarawith APC (GG8-1F3.3.1; Miltenyi Biotec; dilution 1:50) with surface expression detected using a Sony ID7000 Spectral Cell Analyzer. ADGRL4 versions (receptor-SmBiT) and mini-G protein or β-arrestin complementation were assayed using

(162) Functional Characterization of an IL2RG Variant, a Case Report of X-Linked T- B + NK + SCIDImmun Inflamm DisDecember 29, 2025Kristian Assing, Emil Birch Christensen, Christoffer Dellgren, Kerstin Kathrine Soelberg, Christina Fagerberg, Ida Coordt Elle, Bjørk Ditlev Larsen, Dorthe Grosen, Claire Booth, Hanne Vibeke Marquart, et al.for 20 min at 4°C. Subsequently, the cells were washed in FACS buffer and analyzed by flow cytometry (ID7000, Sony Biotechnologies). 2.7 NK Cell Proliferation To assess the proliferation of NK cells in response

(163) Subcellular glycan-mannose receptor binding kinetics correlate with myeloid cell functionNature CommunicationsDecember 26, 2025Kas Steuten, Johannes J. A. Bakker, Ward Doelman, Diana Torres-García, Amit Cherian, Christian Kurts, Roger Riera, Lorenzo Albertazzi, Sander I. van Kasterenuptake and characterization, an identical procedure was followed but acquisition was performed on Sony ID7000 spectral flow cytometer. Analysis was performed using FlowJo v8. Phagocytic index (PI) was calculated

(164) Integrin-Mediated TIMP1 Signaling Reprograms Liver Macrophages and Accelerates Colorectal Cancer MetastasisCellsDecember 23, 2025Jialiang Liu, Liming Zhao, Lin Wang, Guoli Sheng, Pu Cheng, Mingyu Han, Guoxin Li, Zhaoxu Zhengwere then washed three times with Cell Staining Buffer. Fluorescence data were acquired using a Sony ID7000™ flow cytometer (Sony Biotechnology Inc., San Jose, CA, USA) and analyzed with FlowJo™ software (version

(165) Allosteric activation of a cell-type-specific GPR120 inhibits amyloid pathology of Alzheimer’s diseaseNature AgingDecember 19, 2025Aodi He, Yue Wang, Yuhang Shen, Zhiqiang Dong, Lingli Luo, Xiangyu Ge, Xinlu Liu, Yue Mao, Tongmei Zhang, Xinyan Li, et al.centrifuged and rewashed with fluorescence-activated cell sorting buffer for flow cytometry analysis (SONY, ID7000). Data were analyzed using FlowJo software (v10.4, BD Biosciences). Peripheral monocyte-derived macrophages

(166) Anti-CRISPR protein AcrIIA5 can enhance the activity and security of prime editingNat CommunDecember 19, 2025Qi Chen, Xiaoman Jiang, Biao Yang, Zixin Deng, Yuhui Sunadditional times with PBS, and was finally resuspended in PBS containing 0.5% BSA. For flow cytometry (Sony ID7000), 20,000 gated cells were analyzed using 549 nm excitation and 570 nm emission detection (ID7000 Acquisition

(167) Lithium attenuates HIV-1 latency reversal in an autophagy independent wayiScienceDecember 19, 2025Ana Luiza Abdalla, Gabriel Guajardo-Contreras, Bao-An Chau, Meijuan Niu, Thomas Murooka, Andrew J. Moulandnegative control to determine the gag mRNA gate. The samples were acquired using a Spectral Cytometry Sony ID7000. Single colors were used to set the emission spectrum reference (SR). The type 1 probe SR was obtained

(168) Neutrophil Irg1/itaconate axis protects against experimental colitis by suppressing local inflammation and maintaining hematopoietic homeostasisMol BiomedDecember 19, 2025Na Zhao, Guojian Wang, Shuang Long, Yin Chen, Jining Gao, Xiaofan Lv, Xinze Ran, Yi Jia, Tao WangCD11b+Ly6G+ PMNs were detected as previously described [30]. The samples were detected with the Sony ID7000 and BD FAC Symphony flow cytometers. Antibody details are provided in Table S1 and the data were analyzed

(169) Ultrasensitive Test Systems Are Required to Quantify Interferon Alpha Protein in Serum SamplesEur J ImmunolDecember 18, 2025Liis Haljasmägi, Sandra Meisalu, Vincent Bondet, Pärt Peterson, Darragh Duffy, Kai KisandMultiskan MCC/34,ViiA 7 Real‐Time PCR System (ThermoFisher),Victor X Multilabel Plate Reader (Perkin Elmer),ID7000 Spectral Cell Analyzer (Sony Biotechnology),MSD Instrument,Olink Signature Q100 Data analysis software,GraphPad Prism,GraphPad

(170) COX-2/PGE2 axis blockade with celecoxib enhances anti-PD-1 efficacy by activating natural killer cells for residual hepatocellular carcinoma after radiofrequency ablationJ Exp Clin Cancer ResDecember 17, 2025Yu Lei, Yaowei Bai, Xiatong Bai, Bo Sun, Licheng Zhu, Wenlong Wu, Yang Su, Hongsen Zhang, Yingliang Wang, Chuansheng Zhengcells was incubated in the dark for 15 min. Apoptosis rates were determined by flow cytometry (Sony ID7000, Inc). Additionally, the proliferation and migration activities of the remaining viable Hepa1-6 cells

(171) The C2 Domain of PKC‑δ as a Dominant-Negative Modulator of Breast Cancer Cell Survival and ChemosensitivityACS OmegaDecember 15, 2025Rasha Khader, Lodewijk V. DekkerS4). After 15 min RT incubation in the dark, samples were analyzed by spectral flow cytometry (Sony ID7000, Sony Biotechnology, San Jose, CA, USA) and processed with Kaluza software (Beckman Coulter, Brea, CA,

(172) Development of a humanized mouse model of graft-versus-host disease to assess human regulatory T cell functionFront ImmunolDecember 11, 2025Charline Beguin, Oswin Kwan, Grégory Ehx, Stéphanie Humblet-Baron, Murat Cem Köse, Sophie Dubois, Lorenzo Canti, Justine Courtois, Coline Daulne, Jo Caers, et al.ready to be analyzed. Data were acquired on a 5-laser (355 nm, 405 nm, 488 nm, 561 nm, 633 nm) SONY ID7000 Spectral Cell Analyzer (Sony Biotechnology, San Jose, California, USA) and analyzed with FlowJo Software v10.8.1 (Tree Star

(173) Mechanistic insights into single-stranded DNA degradation by lysosomal exonucleases PLD3 and PLD4 from structural snapshotsNat CommunDecember 11, 2025Yoshinori Hirano, Wakiko Ezaki, Ryota Sato, Umeharu Ohto, Kensuke Miyake, Toshiyuki Shimizuusing FACS Aria (BD Bioscience). Post-stimulation with CpG B, cell analysis was conducted using an ID7000 spectral flow cytometer (Sony Biotechnology). The method of normalization involved subtracting the MFI

(174) CD74-Targeting Antibody-Drug Conjugate Enhances Immunosuppression of Glucocorticoid in Systemic Lupus ErythematosusInt J Mol SciDecember 4, 2025Qizhen Du, Shengtao Yao, Yuying Huang, Jia Zhang, Wangmo Sonam, Xiao Lu, Jichao Yang, Shipeng Cheng, Ran Wang, Jiefang Xu, et al.IgG-AF488 (Thermo Fisher Invitrogen, Waltham, MA, USA). The stained cells were analyzed using a Sony ID7000 flow cytometer (Sony, Japan). The proliferation of mouse B cells was analyzed using CellTrace Violet

(175) Periostin Promotes Sarcoma Growth by Promoting Tumor-Associated Macrophage Migration and DifferentiationCancer Res CommunDecember 1, 2025Jin-Fen Xiao, Kristin Ishaya, Emily Y. Ko, Annaliese Fowler, Marina T. Broz, Jlenia Guarnerioare listed in Supplementary Table S1. Flow cytometry and FACS-sorting Cells were analyzed using the ID7000 Spectral Cell Analyzer (SONY), BD FACSymphony (BD), and CytoFLEX LX (Beckman). Cells were sorted using the FACSAria III (BD,

(176) Periostin promotes sarcoma growth by promoting tumor-associated macrophage migration and differentiationCancer Research CommunicationsDecember 1, 2025Jin-Fen Xiao, Kristin Ishaya, Emily Y. Ko, Annaliese Fowler, Marina T. Broz, Jlenia Guarnerioare listed in Supplementary Table S1. Flow cytometry and FACS-sorting Cells were analyzed using the ID7000 Spectral Cell Analyzer (SONY), BD FACSymphony (BD), and CytoFLEX LX (Beckman). Cells were sorted using the FACSAria III (BD,

(177) Reshaping tumor immune microenvironment through ROS-responsive prodrug polyplexes via synergistic effect of CRISPRi system and epigenetic inhibitor for breast cancer therapyMaterials Today BioDecember 1, 2025Huan Deng, Qianru Li, Bingxu Wang, Hong Yu, Shouzheng Sun, Zichen Li, Weizhen Pan, Qianfu Zhao, Heshuang Dai, Jiao Lu, et al.APC-CD11c, BV421-CD86, and PE-CD80 antibodies. All the lymphocytes were detected by flow cytometer (Sony ID7000™ Spectral Cell Analyzer) and analyzed using FlowJo software. The expression levels of cytokines, including interleukin-6 (IL-6),

(178) Flow Cytometry: Advances, Challenges and TrendsBioEssaysNovember 27, 2025J. Paul Robinson, Grzegorz B. Gmyrek, Bartek Rajwadetectors (APDs), which was later upgraded to five lasers and 64 detectors. In 2019, Sony launched the ID7000 spectral cytometer with seven lasers and 184 detectors, featuring grating‐based spectral dispersion,

(179) Microbial influx during early postnatal life fortifies the ocular surface and guards against allergic eye disease in miceCommunications BiologyNovember 26, 2025Yanbo Liu, Hang Sun, Shulin Song, Xiezhou He, Han Wu, Shangkun Ou, Hui He, Rongrong Zong, Yongxiong Chen, Guo Fu, et al.Pharmingen, 562574) and subsequently stained with IL-4 and IFN-γ antibodies. Data were acquired on SONY ID7000™ Spectral Cell Analyzer using the ID A analysis software (v2.0; SONY). Data were analyzed on FlowJo software (FlowJo LLC, Ashland,

(180) A viral codon usage strategy enhances antigen production and protection in SFTSV mRNA vaccinationnpj VaccinesNovember 24, 2025Inho Cha, Yumiko Yamada, Dokyun Kim, Soowon Kang, Wan-Shan Yang, Woo-Jin Shin, Anna Ryder, Morgan Lewis, Chloe Chung, Nam-Hyuk Cho, et al.Tandem Stabilizer (BioLegend). Cells were stored at 4 °C in the dark prior to acquisition on a SONY ID7000 Spectral Cell Analyzer (Sony Biotechnology). Flow cytometric data were analyzed using FlowJo (v10.9.0, Treestar Inc.). The

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ID7000™ Spectral Cell Analyzer

ID7000™ Spectral Cell Analyzer from Sony Corporation

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ID7000™ Spectral Cell Analyzer from Sony Corporation

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