Intracellular Staining Perm Wash Buffer (10X) from BioLegend

Product Specs
ItemIntracellular Staining Perm Wash Buffer (10X)
CompanyBioLegend
Price $94.00Supplier Page View Company Product Page
Catalog Number421002
ApplicationsICC, ICFC - Quality tested, IHC - Reported in the literature, not verified in house
Quantity100 mL

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Microneedle-based injection of Fungizone/Amphotericin B: an effective treatment for American cutaneous leishmaniasis in miceDrug DelivDecember 31, 2026Ryan H. Huston, Blake Cox, Chaitenya Verma, Thalia Pacheco-Fernandez, Greta Volpedo, M. Junaid Dar, Yulian Mercado, Abigail R. Wharton, Max Gessner, Nandu Purayil, et al.Subsequently, the cells were permeabilized with 1x Intracellular Staining Perm Wash Buffer (BioLegend cat# 421002), and the intracellular cytokines and isotype controls for these antibodies were stained (1 µL per 1

(2) Modeling stimulus-induced stress responses in microglia-like cells using a commercial iPSC-dCas9-KRAB lineStem Cell ResearchAugust 1, 2026Devin M. Saunders, Nicola A. Kearns, Bernard Ng, Faraz Sultan, Himanshu Vyas, Ricardo A. Vialle, Eric M. Clark, Sashini De De Tissera, Jishu Xu, David A. Bennett, et al.fixed cells were permeabilized using Intracellular Staining Permeabilization Wash Buffer (Biolegend, 421002) according to the manufacturer’s instructions and cells were stained for intracellular markers according

(3) Oncolytic adenovirus armed with cGAS activates STING pathway and enhances antitumor immunity in lung cancer with superior combined efficacy of PD-L1 therapyFront ImmunolJuly 23, 2026Qing-Wen Wang, Hua-Wei Xu, Yu-Sen Shi, Yi-Peng Zhang, Jie Jun, Dan-Ning Yue, Wei Zhao, Jia-Qiang Huang, Xiang-Lei Peng, Jie-Mei Yu, et al.cells were then resuspended in diluted Intracellular Staining Permeabilization Wash Buffer (BioLegend, 421002, USA), and centrifuged at 350 × g for 5–10 min; this step was repeated twice. Finally, IFN-γ-conjugated

(4) Dendritic cell redundancy enables priming of anti-tumor CD4+ T cells in pancreatic cancerCancer CellJuly 13, 2026Courtney T.S. Kureshi, Michael J. Walsh, Rakeeb Kureshi, Victoire Cardot-Ruffino, Dennis A. Agardy, Lestat R. Ali, James M. Dougan, Li Qiang, Jiao Shen, Chong Zuo, et al.Supernatants were discarded and cells resuspended in 1 mL of permeabilization/wash buffer (BioLegend #421002). Cells were centrifuged at 700 g for 5 min. This step was repeated, and then cells were stained with

(5) Intermediate accumulated upon interruption of fatty acid oxidation flux promotes tumoral ferroptosis and improves immunotherapyNature CommunicationsJune 17, 2026Yuhan Zhou, Jing Li, Fangfang Liu, Yuan Gao, Songlin Yin, Liguo Yang, Xiaoxiao Li, Junhong Lin, Yan Li, Haotian Shang, et al.Cells were washed, fixed and permeabilized by Fixation Buffer (420801, Biolegend) and Perm/Wash Buffer (421002, Biolegend), respectively. Then, cells were stained with anti-IFNγ (XMG1.2, Biolegend) and anti-TNFα

(6) Case Report: Immune checkpoint inhibitor-associated myocarditis, myositis, and myasthenia gravis overlap syndrome with flow cytometric phenotyping before and after treatment in a patient with urothelial carcinomaFrontiers in ImmunologyJune 15, 2026Karen Gambina, Carly Tymm, Matthieu Paiola, Kurenai Tanji, John Zech, Robert Winchester, Adam Mor, Yevgeniya GartshteynFixation Buffer (BioLegend #420801) and treated with Intracellular Staining Fixation Buffer (BioLegend 420801 and 421002) before intracellular staining for granzyme B and SAP. Spectral flow data was obtained on the Cytek

(7) The Effects of CB2R Activation on Inflammatory Pathways in DermatomyositisBiomedicinesJune 7, 2026Rohan Dhiman, Ahmed Eldaboush, Navin Vijayarangan, Darae Kang, Nilesh Kodali, DeAnna Diaz, Caroline Stone, Rui Feng, Victoria P. Werth(Biolegend USA, #420801). This was then followed by resuspension in permeabilization buffer (Biolegend USA, #421002). The following antibodies were added to all tubes: PE CD68 (Biolegend USA, #333808) and BUV 661 CD163

(8) Effect of the JAK Inhibitor Baricitinib on Cytokine Production and Bone Properties in a Mouse Model of Accelerated AgingInt J Mol SciJune 3, 2026Katharina Gelles, Vincent Kurz, Maria Butylina, Katharina Wahl-Figlash, Martin Schepelmann, Anastasia Meshcheryakova, Peter Pietschmannby washing twice with Perm/Wash (Intracellular Staining Permeabilization Wash Buffer 10×; BioLegend, 421002). Meanwhile, different combinations of antibodies were prepared in Perm/Wash. Cells were resuspended

(9) NMI Regulates Adipose Adaptive Thermogenesis Through TLR4/IRF3 Signaling to Promote ObesityAdvanced ScienceJune 2, 2026Ting‐Ting Li, Xin‐Yuan Zhao, Min Zhang, Zhuang‐Feng Weng, Xiao‐Ran Guo, Ying‐Fang Liu, Qiao‐Ping Wang, Huan‐Huan Liangincubation with antibodies diluted in intracellular permeabilization wash buffer (Biolegend, USA, Cat#421002). The following antibodies were used: FITC‐conjugated anti‐mouse CD45 (Cat#157214) and PerCP/Cy5.5‐conjugated

(10) NQO1‐Mediated Anoikis Resistance and Immune Evasion Define a High‐Risk Multi‐Omic Subtype for Precision Management of T1 High‐Grade Bladder CancerAdvanced ScienceJune 1, 2026Bin Guo, Chunru Xu, Shufan Fu, Qiang Cheng, Juan Li, Linkuo Shang, Gaojie Li, Yang Yang, Ying Wang, Yanqing Gong, et al.permeabilization with the Intracellular Staining Permeabilization Wash Buffer (10X) (BioLegend, Cat. no. 421002). 4.7 Animal Experiments All protocols for mice were approved by the Animal Ethics Committee of Beijing

(11) Multimodal sequencing identifies synergistic mechanisms driving resistance to neoadjuvant nivolumab treatment in hepatocellular carcinomaMolecular CancerJune 1, 2026Fanhong Zeng, Jiaxin Guo, Zheng Zeng, Vanilla Xin Zhang, Yu-Man Tsui, Po-Man Li, Abdullah Husain, Qingyang Zhang, Joyce Man-Fong Lee, Tan-To Cheung, et al.Compensation Beads,BioLegend,424601 Fixation Buffer,BioLegend,420801 Intracellular Staining Permeabilization Wash Buffer (10X),BioLegend,421002 Information on antibodies and reagents for flow cytometry

(12) IL-21 isoform transgenic mice spontaneously develop mammary tumors: possible involvement of IL-21-induced osteopontin expression in tumorigenesisFrontiers in ImmunologyJune 1, 2026Risako Yamaguchi, Akemi Araki, Yuji Takeda, Shinichi Saitoh, Junji Yokozawa, Masahiro Yamamoto, Naing Ye Aung, Mitsuru Futakuchi, Satoru Nagase, Hironobu Asao#554722), followed by staining with labeled antibodies in Intracellular Staining Wash Buffer (BioLegend, #421002) in the presence of an Fc-blocking antibody (anti-CD16/32, clone 2.4G2). Ki67 staining was performed

(13) Exhausted T cell phenotypes in disseminated coccidioidomycosisJCI InsightMay 22, 2026Gregory D. Whitehill, Alexis V. Stephens, Timothy J. Thauland, Miguel A. Moreno Lastre, Matthew M. Tate, Sinem Beyhan, Royce H. Johnson, George R. Thompson, Maria Garcia-Lloret, Manish J. Butteparaformaldehyde in PBS prior to surface staining, then permeabilized with Perm Buffer (BioLegend, 421002). Intracellular cytokine staining was performed in Perm Buffer at 4°C for 35 minutes. Cells were then

(14) Th2 skewing in patients with disseminated coccidioidomycosisJCI InsightMay 22, 2026Timothy J. Thauland, Smriti S. Nagarajan, Alexis V. Stephens, Samantha L. Jensen, Anviksha Srivastava, Miguel A. Moreno Lastre, Terrie S. Ahn, Chantana Bun, Michael T. Trump, Royce H. Johnson, et al.Cells were then fixed with PBS/2% paraformaldehyde and permeabilized with 1× Perm Buffer (BioLegend, 421002). Cells were stained in Perm Buffer with anti–IFN-γ (BioLegend, clone 4S.B3), anti–IL-4 (BioLegend,

(15) Macrophage-derived fibronectin suppresses antitumor immunity via tissue stiffening and immunosuppressive cell induction in cancer mouse modelsNature CommunicationsMay 22, 2026Aitian Li, Ying Wang, Haiqing Bai, Xin Xie, Beibei Xu, Yunhan Wang, Shengjun Luo, Lei Zhang, Qitai Zhao, Shichao Duan, et al.Following the fixation/permeabilization steps using an intracellular staining perm wash buffer (BioLegend, 421002), intracellular staining was performed using fluorescence-labeled antibodies (e.g., CD206, IL-6, TNF,

(16) Peroxisomal ABCD1 deficiency in mice drives Th1 bias through 25-HC–LXR signaling in CD4+ T cellsFrontiers in ImmunologyMay 20, 2026Reina Maeda, Mayu Oishi, Ryutaro Nagamori, Mari Hikosaka-Kuniishi, Shiro Watanabe, Jung-Bum Lee, Masashi Morita, Takanori Somin, fixed with 2% paraformaldehyde for 20 min, and permeabilized using buffer solutions (BioLegend 421002). Following blocking with Rat IgG1, cells were stained intracellularly with an APC-IFN-γ antibody or

(17) Evaluation of Safety and Immunogenicity of High-Dose Quadrivalent Seasonal Influenza Split Vaccine: A Preclinical StudyVaccines (Basel)May 17, 2026Lanxin Jia, Ran Qiu, Jing Liu, Bo Liu, Xuanxuan Nian, Yang Le, Xixin Han, Qingmei Zhang, Xuedan Li, Zheng Gong, et al.resuspended in 1 mL of Intracellular staining perm buffer (BioLegend, San Diego, CA, USA; Cat. No. 421002) for permeabilization. Following centrifugation (300× g for 5 min), intracellular staining was conducted

(18) Activation-gated, T cell-restricted silencing of Dapk1 enhances Bacille Calmette-Guérin-elicited protective CD4+ memoryiScienceMay 15, 2026Zixuan Liu, Weihuang Liu, Chenyu Zhao, Xuanchang Bai, Fangting Zhou, Hang Sun, Jiahe Shi, Pinru Chen, Min Liu, Qin Pan423105 Fixation Buffer,Biolegend,Cat# 420801 Intracellular Staining Perm Wash Buffer(10×),Biolegend,Cat# 421002 Brefeldin A,Absin,Cat# abs810012 Cell lysis buffer,Beyotime,Cat# P0013 Fixable Viability Stain 780

(19) Ophiopogonin D Mediates Therapeutic Effects on Primary Sjögren's Syndrome by Modulating Gut Microbiota and Metabolite ProfilesBiomed ChromatogrMay 12, 2026Fengtao Pang, Xueyan Shan, Yi Kong, Xiaopo Tangacquired from BioLegend (420601); and the Intracellular Staining Buffer Kit was purchased from BioLegend (421002). 2.2 Experimental Animals This study used eight female BALB/c mice and 24 female NOD/LtJ mice, all

(20) Development of a nano-emulsion and evaluation of its intradermal adjuvant function of Swine Influenza H3N2 VaccineVirology JournalMay 8, 2026Zhuoyu Ruan, Bihua Deng, Wenzhu Yin, Fang Ma, Yu Lu, Haiyan Wangstaining, cells were fixed and permeabilized using a commercial fixation/permeabilization kit (420801, 421002, BioLegend), and then stained intracellularly with an antibody against mouse IFN-γ (BV421, 505830, BioLegend).

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2/13/2020Easy-to-Use Intracellular Staining Permeabilization Wash Buffer Sunita MeenaOverall best product.

How to maximize your Treg research: from fixation/permeabilization buffers to Nanobodies
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In defending the human body from invading pathogens, the immune system has to walk a fine line between reacting to foreign antigens and over-reacting to foreign antigens. To help modulate this response the immune system... read more

Intracellular Staining Perm Wash Buffer (10X) from BioLegend

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How to maximize your Treg research: from fixation/permeabilization buffers to Nanobodies

Intracellular Staining Perm Wash Buffer (10X) from BioLegend

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