Intracellular Staining Perm Wash Buffer (10X) from BioLegend

Product Specs
ItemIntracellular Staining Perm Wash Buffer (10X)
CompanyBioLegend
Price $94.00Supplier Page View Company Product Page
Catalog Number421002
Quantity100 mL
ApplicationsICC, ICFC - Quality tested, IHC - Reported in the literature, not verified in house

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Microneedle-based injection of Fungizone/Amphotericin B: an effective treatment for American cutaneous leishmaniasis in miceDrug DelivDecember 31, 2026Ryan H. Huston, Blake Cox, Chaitenya Verma, Thalia Pacheco-Fernandez, Greta Volpedo, M. Junaid Dar, Yulian Mercado, Abigail R. Wharton, Max Gessner, Nandu Purayil, et al.Subsequently, the cells were permeabilized with 1x Intracellular Staining Perm Wash Buffer (BioLegend cat# 421002), and the intracellular cytokines and isotype controls for these antibodies were stained (1 µL per 1

(2) Evaluation of Safety and Immunogenicity of High-Dose Quadrivalent Seasonal Influenza Split Vaccine: A Preclinical StudyVaccines (Basel)May 17, 2026Lanxin Jia, Ran Qiu, Jing Liu, Bo Liu, Xuanxuan Nian, Yang Le, Xixin Han, Qingmei Zhang, Xuedan Li, Zheng Gong, et al.resuspended in 1 mL of Intracellular staining perm buffer (BioLegend, San Diego, CA, USA; Cat. No. 421002) for permeabilization. Following centrifugation (300× g for 5 min), intracellular staining was conducted

(3) Activation-gated, T cell-restricted silencing of Dapk1 enhances Bacille Calmette-Guérin-elicited protective CD4+ memoryiScienceMay 15, 2026Zixuan Liu, Weihuang Liu, Chenyu Zhao, Xuanchang Bai, Fangting Zhou, Hang Sun, Jiahe Shi, Pinru Chen, Min Liu, Qin Pan423105 Fixation Buffer,Biolegend,Cat# 420801 Intracellular Staining Perm Wash Buffer(10×),Biolegend,Cat# 421002 Brefeldin A,Absin,Cat# abs810012 Cell lysis buffer,Beyotime,Cat# P0013 Fixable Viability Stain 780

(4) Ophiopogonin D Mediates Therapeutic Effects on Primary Sjögren's Syndrome by Modulating Gut Microbiota and Metabolite ProfilesBiomed ChromatogrMay 12, 2026Fengtao Pang, Xueyan Shan, Yi Kong, Xiaopo Tangacquired from BioLegend (420601); and the Intracellular Staining Buffer Kit was purchased from BioLegend (421002). 2.2 Experimental Animals This study used eight female BALB/c mice and 24 female NOD/LtJ mice, all

(5) LGALS9 blockade augments vaccine-induced immune responses against prostate cancerJ Immunother CancerMay 8, 2026Bowen Lu, Ning Liu, Wanting Zhao, Haomin Chen, Yingxiang Shao, Haosen Lu, Shanshan Liu, Jie Yang, Yanyan Zheng, Jiage Ding, et al.(Biosharp, BL539A) for 15 min at room temperature, washed with 1×Permeabilization Wash Buffer (BioLegend, 421002), and incubated with antibodies diluted in permeabilization buffer at 4°C overnight. For intracellular

(6) Endogenous CD28 drives the persistent activity of CAR T cells in myeloma and lymphoma modelsBlood Cancer DiscovMay 5, 2026Mackenzie M. Lieberman, Jason H. Tong, Nkechi U. Odukwe, Colin A. Chavel, Gina G. Bishara, Kimberly M. Crasti, Megan M. Herr, Payal Goala, Terence J. Purdon, Rebecca Burchett, et al.fixation and permeabilization according to manufacturer’s instructions (BioLegend, catalog #420801 & #421002). Antibodies used in phenotypic analysis are included in the Key Resources Table and gating strategies

(7) Novel NK cell-like phenotype expressing CCR5 and its ligands elicits tumor-specific acquired immunity to carcinomatous peritonitis via host NK-derived IFN-γJ Immunother CancerApril 20, 2026Situo Zheng, Yui Harada, Yosuke Morodomi, Noriko Yasuda, Kenta Ishimoto, Yoshikazu Yonemitsuroom temperature, then washed two times using 1x Intracellular Staining Perm Wash Buffer (BioLegend, 421002, California, USA) and resuspended in phosphate-buffered saline (PBS) for IFN-γ staining. Supernatant

(8) Enhanced Th1 Cellular Immunity Induced by an RSV-F mRNA Vaccine Rationally Designed Using NLP AlgorithmsVaccines (Basel)April 16, 2026Zhi-Wu Xia, Qi Tang, Jun-Jie Pan, Jing Liu, Lan-Xin Jia, Guo-Mei Zhang, Man-Ni Xie, Jia-Hao Zheng, Chuan-Shuo Lv, Lei Zhang, et al.#420801) and permeabilization (Biolegend Intracellular Staining Permeabilization Wash Buffer, Cat. #421002), intracellular cytokines were stained with PE-conjugated anti-mouse IL-2 (Biolegend, Cat. #503808),

(9) Phenanthrene Exposure Induces Immune Dysfunction and Metabolic Disruption in MiceACS OmegaApril 15, 2026Qiaojuan Liu, Yan Zhang, Fenfang Deng, Wenxue Li, Guangyu Yang, Jun Yuan, Yuehong Wei, Juntao Li, Lei Tanpermeabilized using the Intracellular Fixation/Permeabilization Buffer Set (BioLegend, Cat. No. 420801/421002), followed by staining with fluorochrome-labeled antibodies against IFN-γ and TNF-α. All stained cells

(10) Role of Th17/Treg immune balance in assessment of mycoplasma pneumoniae pneumonia severity and its discriminatory value for asthma in children with mycoplasma pneumoniae pneumoniaBMC PediatricsMarch 25, 2026Danhong Qian, Yingying Chen, Xinxia Miao, Fanyou Mengantibody (320007), True-Nuclear™ Transcription Factor Buffer Set (424401), permeabilization wash buffer (421002), and Cell Activation Cocktail (423303) were purchased from BioLegend (San Diego, CA, USA). Enzyme-Linked Immunosorbent Assay

(11) Recombinant human adenovirus type 5 synergizes with anti-PD-L1 antibody to promote anti-hepatocellular carcinoma effects through multilevel remodeling of the immune microenvironmentFrontiers in ImmunologyMarch 24, 2026Qiu Zhao, Min Xiao, Jian Ma, Xiaoqian Li, Cong Fu, Wenjing Ji, Lingna Ni, Jialin Fan, Qianqian Gao, Yanzhi Bifixation buffer (BioLegend, 420801), and intracellular staining permeabilization wash buffer (BioLegend, 421002). 2.7 HE, immunohistochemistry The tumor tissue was fixed with 4% paraformaldehyde, then dehydrated,

(12) The AICL-KLRF1 axis supports CD4-CD8 T cell communication and cytokine competence in pre-exhausted CD8+ T cellsEMBO ReportsMarch 18, 2026Matthias Barone, Stefan Peidli, Anika Neuschulz, Karla Riesterer, Christina Iwert, Laia Junquera, Somesh Sai, Olufemi Bolaji, Diana Bakoueva, Christine Appelt, et al.Zombie UV Fixable Viability Kit,BioLegend,423107 Beriglobin,CSL Behring GmbH,discont. Cytofix/Cytoperm,BD Biosciences,51-2090KZ Perm/Wash,BioLegend,421002 AO/PI Assay_CellDrop FL,DeNovix,CD-AO-PI-1-5-C Sodium Azide,Sigma-Aldrich,S2002-100G Xylene,VWR,28973.363 Ethanol,Carl

(13) Regulation of MRGPRX2-mediated mast cell function by competence-stimulating peptide 1 and pro-adrenomedullin peptideFrontiers in ImmunologyMarch 17, 2026Aetas Amponnawarat, Sangita Sutradhar, Chalatip Chompunud Na Ayudhya, Maram Bawazir, Ronit Sagi-Eisenberg, Hydar Alianti-HA antibody (Clone Poly9023; Catalog #923501), and Intracellular Staining Perm Wash Buffer (Catalog #421002) were purchased from BioLegend. AF647-conjugated donkey anti-mouse IgG (Catalog #A31571), AF488-conjugated

(14) Comprehensive single-cell metabolic profiling identifies targets to sensitize triple-negative breast cancer to chemo-immunotherapyCell Rep MedMarch 17, 2026Ying Xu, Xin-Yi Liu, Hang Zhang, Li Chen, Han Wang, Zhi-Ming Shao, Yi Xiao, Yi-Zhou JiangRBC Lysis Buffer (10x),BioLegend,Cat# 420302 Intracellular Staining Permeabilization Wash Buffer (10x),BioLegend,Cat# 421002 Fixation Buffer,BioLegend,Cat# 420801 Cyto-Last™ Buffer,BioLegend,Cat# 422501 Cell Staining Buffer,BioLegend,Cat#

(15) Regulation of MRGPRX2-mediated mast cell function by competence-stimulating peptide 1 and pro-adrenomedullin peptideFront ImmunolMarch 17, 2026Aetas Amponnawarat, Sangita Sutradhar, Chalatip Chompunud Na Ayudhya, Maram Bawazir, Ronit Sagi-Eisenberg, Hydar Alianti-HA antibody (Clone Poly9023; Catalog #923501), and Intracellular Staining Perm Wash Buffer (Catalog #421002) were purchased from BioLegend. AF647-conjugated donkey anti-mouse IgG (Catalog #A31571), AF488-conjugated

(16) Selenium-loaded sustained-release schizophyllan alleviates pancreatic and pulmonary inflammatory damage in type 1 diabetes mellitus by modulating gut microbiota and T cell balanceJournal of NanobiotechnologyMarch 14, 2026Junming Luo, Siying Pei, Zhiying Ai, Runqing Rao, Zhuoyuan Yang, Xin Guo, Zeyi Ai, Lilin Fan, Fang ZouBioLegend, USA) mixed with diluent at a 1:3 ratio, followed by treatment with permeabilization buffer (421002, BioLegend, USA). Cells were then stained with Interferon gamma (IFN-γ)-PE (505808, BioLegend, USA;

(17) Context‐Dependent Role of GDF15: GDF15 + Tumor‐Associated Macrophages Suppress OSCC Progression by Enhancing PhagocytosisAdvanced ScienceMarch 13, 2026Xinyu Zhou, Zhihang Zhou, Houyu Ju, Chaoyang Guo, Fangxing Zhu, Yiyi Zhang, Xu Chen, Tongchao Zhao, Laiping Zhong, Jingzhou Hutemperature, followed by permeabilization using intracellular staining permeabilization wash buffer (421002, BioLegend, USA). Intracellular staining was performed on ice overnight. As for the staining of GDF15+

(18) Prolonged Wnt3a exposure tolerizes macrophages to inflammatory stimuliFront ImmunolMarch 12, 2026Megan L. Tigue, Rincon Jagarlamudi, Channing Chi, Diana Diaz, Hua-Chang Chen, Quanhu Sheng, Sheau-Chiann Chen, Anna Schwarzkopf, Jeremy A. Goettel, Heather H. Pua, et al.minutes in the dark and resuspended in 1X Intracellular Staining Permeabilization Wash Buffer (BioLegend, 421002). Cells were then stained with APC anti-mouse IL-6 Antibody [MP5-20F3] (BioLegend, 504507), FITC anti-mouse TNF-α

(19) Gelatinase regulates the egress of intracellular replicating populations during Enterococcus faecalis infectionPLoS PathogMarch 10, 2026Frederick Reinhart Tanoto, Jia Hui Liew, Claudia J. Stocks, Deepti Rawat, Kelvin Kian Long Chong, Kevin Pethe, Haris Antypas, Kimberly A. Klinestaining by washing thrice with Intracellular Staining Permeabilization Wash Buffer (BioLegend, Cat# 421002, USA; hereby called permeabilization buffer). Then, cells were stained with 1:500 rabbit anti-AgD diluted

(20) NR2F6 deletion revives CAR-T cell function and induces antigen-agnostic immune memory in solid tumorsNature CommunicationsFebruary 27, 2026Dominik Humer, Victoria Klepsch, Dietmar Rieder, Isabel Hölzl, Daniel Schreiber, Viktor Lang, Jiří Koutník, Sebastian Peer, Tajana Sajinovic, Viana Wille, et al.fixation, cells were permeabilized by washing twice with the kit’s Perm/Wash buffer (1x, Biolegend, 421002). Intracellular proteins were stained for at least 30 minutes to one hour at 4 °C in Perm/Wash buffer

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2/13/2020Easy-to-Use Intracellular Staining Permeabilization Wash Buffer Sunita MeenaOverall best product.

How to maximize your Treg research: from fixation/permeabilization buffers to Nanobodies
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In defending the human body from invading pathogens, the immune system has to walk a fine line between reacting to foreign antigens and over-reacting to foreign antigens. To help modulate this response the immune system... read more

Intracellular Staining Perm Wash Buffer (10X) from BioLegend

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BioLegend

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How to maximize your Treg research: from fixation/permeabilization buffers to Nanobodies

Intracellular Staining Perm Wash Buffer (10X) from BioLegend

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