Intracellular Staining Perm Wash Buffer (10X) from BioLegend

Product Specs
ItemIntracellular Staining Perm Wash Buffer (10X)
CompanyBioLegend
Price $94.00Supplier Page View Company Product Page
Catalog Number421002
ApplicationsICC,ICFC,IHC
Quantity100ML

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Microneedle-based injection of Fungizone/Amphotericin B: an effective treatment for American cutaneous leishmaniasis in miceDrug DelivDecember 31, 2026Ryan H. Huston, Blake Cox, Chaitenya Verma, Thalia Pacheco-Fernandez, Greta Volpedo, M. Junaid Dar, Yulian Mercado, Abigail R. Wharton, Max Gessner, Nandu Purayil, et al.Subsequently, the cells were permeabilized with 1x Intracellular Staining Perm Wash Buffer (BioLegend cat# 421002), and the intracellular cytokines and isotype controls for these antibodies were stained (1 µL per 1

(2) High-Density Type I Collagen Promotes IFN-γ+ CD8+ T Cell Exhaustion via SAT1-ASS1-Mediated Glutamine Accumulation and Ferroptosis in Triple-Negative Breast CancerAdv Sci (Weinh)September 27, 2026Jinyan Wang, Shuangyue Pan, Lu Bai, Bin Li, Tiantian Liu, Jianing Cao, Mengdi Yang, Heda Zhang, Duancheng Guo, Wenxiang Zhi, et al.against cell surface markers, cells were fixed and permeabilized using the relevant buffer set (420801, 421002, BioLegend, USA) for 20 min to disrupt the cell membrane. They were then treated with fluorochrome‐conjugated

(3) Extracellular matrix proteoglycans lumican and biglycan promote innate immune signals and viral clearance in HSV-1 infections of mouse corneasbioRxivSeptember 18, 2026George Maiti, Madhuri A Koduri, Elizabeth Shin, Yoonchul Shin, Trisha Sinha, Alireza Khodadadi-Jamayran, Hongmin Yun, Anthony J. St. Leger, Shukti ChakravartiSerum,Sigma-Aldrich,Cat # G9023 Intracellular Staining Permeabilization Wash Buffer,BioLegend,Cat # 421002 Crystal Violet,Sigma-Aldrich,Cat # C0775-25G Sodium Carboxymethyl cellulose,Sigma-Aldrich,Cat # 419273-100G Critical

(4) A novel p53R273H-Selective Bispecific T-Cell Engager: from Clinical TCR clone to Computational Optimization and Functional ValidationMolecular Therapy OncologySeptember 17, 2026Nhan Huynh, Thu-My Thi Nguyen, Ngoc Thi Bui, Thao-Nguyen Ho Nguyen, Diem T.P. Tran, Hoai-Nghia Nguyen, Cuong Thach Nguyenwere then permeabilized using Intracellular Staining Permeabilization Wash Buffer (BioLegend, cat. no. 421002) for 45 min, washed twice, and stained with the relevant antibodies. Flow cytometry data were acquired

(5) Inhibiting CXCR2 Remodels the Tumor Microenvironment and Blunts Tumor Progression and Dissemination of High-Grade Serous CarcinomaCancer Res CommunSeptember 1, 2026Railey G. Mikeska, Lily Elizabeth R. Feldman, Elizabeth R. Woodruff, Ritsuko Iwanaga, Katharine E. Linder, Tomomi M. Yamamoto, Kayla Sompel, Kimberly R. Jordan, Miriam D. Post, Maia Zoller, et al.were then permeabilized with Intracellular Staining Permeabilization Wash Buffer (BioLegend, cat. #421002) for 5 to 10 minutes. After fixation and permeabilization, cells were stained with a PE fluorophore-conjugated

(6) Pharmacological Inhibition of Small Extracellular Vesicle Secretion by ALK5i SD-208 via Lysosomal Rerouting of CD63+ CompartmentsJ Extracell VesiclesAugust 29, 2026Rahul Sanwlani, Georgina H. Thompson, Kyle Bramich, Ching-Seng Ang, Millie L. Trowbridge, Chris E. Duringer, Vlad Stolojan, Sean M. Davidson, Konstantinos Savvatis, John H. McVey, et al.washed and permeabilised using 100 µL of 1× Intracellular Permeabilization (IP) Buffer (BioLegend, 421002), followed by a second centrifugation step (400 × g, 5 min). This wash‐permeabilisation cycle was repeated

(7) A generalizable covalent car-t platform for solid tumors enabled by oncolytic adenovirus-delivered artificial antigensFrontiers in ImmunologyAugust 26, 2026Linpei Guo, Hui Xie, Di Yin, Guidong Zhu, Peichao Zhai, GuoJing Pei, Wen Zhang, Fang Xiao, Jiaju Lyu, Dongqi Tangwith brefeldin A (BioLegend # 420601) for 12 h, fixed (BioLegend, #420801), permeabilized (BioLegend, #421002), and processed according to the manufacturer’s instructions. Cells were then incubated antibodies for

(8) Pulmonarom® promotes TLR-related and interferon-related antiviral responses during influenza A (H1N1 and H3N2) infection in human monocyte-derived dendritic cellsFrontiers in Cellular and Infection MicrobiologyAugust 18, 2026Sandra Georgina Solano-Gálvez, Juan A. Hernández-Aceves, Alberto García Lozano, Arturo A. Wilkins-Rodríguez, Diego Alexander Rojas Ortega, Antonio Ibarra, Exsal Manuel Albores Méndez, Edda Sciutto, Laila Gutiérrez-Kobeh, Horacio Merchant Larios, et al.(BioLegend, Cat. 420801) for 20 min at room temperature, then washed with 1× Wash Buffer (BioLegend, Cat. 421002) and centrifuged twice at 1500 rpm for 5 min. Cells were incubated overnight (~12 h) in 1× Wash Buffer

(9) Enhancing iNKT cell immunotherapy through the integration of optimized CAR endodomains and iNKT engagersNature CommunicationsAugust 14, 2026Kanagaraju Ponnusamy, Klesti Karaxhuku, Yuchao Jiang, Lyra Randzavola, Hongwei Ren, Ilia Leontari, Bryan Lye, Mehmood Zaidi, Edward J. Bartlett, Edward W. Tate, et al.fixed and permeabilized by following standard manufacturer’s instructions (BioLegend, cat# 420801 and 421002). The cells were stained with granzyme B (clone: GB11) and IFNγ (clone: 4S.B3) in FACS buffer for 30

(10) AFF3 maintains metabolic quiescence in naïve CD8 T cells and prevents premature immune agingJCI InsightAugust 10, 2026Molly E. Lumnitzer, Stefanie F. Valbon, Stephanie A. Condotta, Allison E. Norlander, Sheng Liu, Jun Wan, Martin J. Richerwas performed as described above. Fixed cells were washed with 1x permeabilization buffer (BioLegend, 421002) and stained with intracellular antibody diluted in 1x permeabilization buffer and incubated at 4°C

(11) Cblb Gene Editing in T Cells Sustains Expansion and Immunogenic CAR T Tumor Killing Under Chronic Antigenic StimulationCellsAugust 3, 2026Daniel Schreiber, Sebastian Peer, Christina Lutz-Nicoladoni, Jiří Koutník, Viktor Lang, Viana Wille, Isabel Hölzl, Dominik Humer, Nino Tokic, Dorothee Freimark, et al.washed twice with 1× Intercellular Staining Permeabilization Wash Buffer (Perm/Wash Buffer, BioLegend, 421002) for permeabilization of cells. Staining of cytokines was performed for 30 min at 4 °C using a cytokine

(12) Modeling stimulus-induced stress responses in microglia-like cells using a commercial iPSC-dCas9-KRAB lineStem Cell ResearchAugust 1, 2026Devin M. Saunders, Nicola A. Kearns, Bernard Ng, Faraz Sultan, Himanshu Vyas, Ricardo A. Vialle, Eric M. Clark, Sashini De De Tissera, Jishu Xu, David A. Bennett, et al.fixed cells were permeabilized using Intracellular Staining Permeabilization Wash Buffer (Biolegend, 421002) according to the manufacturer’s instructions and cells were stained for intracellular markers according

(13) Oncolytic adenovirus armed with cGAS activates STING pathway and enhances antitumor immunity in lung cancer with superior combined efficacy of PD-L1 therapyFront ImmunolJuly 23, 2026Qing-Wen Wang, Hua-Wei Xu, Yu-Sen Shi, Yi-Peng Zhang, Jie Jun, Dan-Ning Yue, Wei Zhao, Jia-Qiang Huang, Xiang-Lei Peng, Jie-Mei Yu, et al.cells were then resuspended in diluted Intracellular Staining Permeabilization Wash Buffer (BioLegend, 421002, USA), and centrifuged at 350 × g for 5–10 min; this step was repeated twice. Finally, IFN-γ-conjugated

(14) Dendritic cell redundancy enables priming of anti-tumor CD4+ T cells in pancreatic cancerCancer CellJuly 13, 2026Courtney T.S. Kureshi, Michael J. Walsh, Rakeeb Kureshi, Victoire Cardot-Ruffino, Dennis A. Agardy, Lestat R. Ali, James M. Dougan, Li Qiang, Jiao Shen, Chong Zuo, et al.Supernatants were discarded and cells resuspended in 1 mL of permeabilization/wash buffer (BioLegend #421002). Cells were centrifuged at 700 g for 5 min. This step was repeated, and then cells were stained with

(15) Precise control of switchable chimeric antigen receptor T cells allows enhanced safety and less T cell exhaustionJournal of Translational MedicineJune 17, 2026Zihan Anna Zhang, Logan Herring, Thuzar Hla Shwe, Yue Hu, Xiaotong Song, Wenyue Cao, Wenshe Ray Liuwere permeabilized and labelled with intracellular staining permeabilization wash buffer (Biolegend, 421002) according to the manufacturer’s instructions. Cytotoxicity assay Cytotoxicity assays were conducted

(16) Intermediate accumulated upon interruption of fatty acid oxidation flux promotes tumoral ferroptosis and improves immunotherapyNature CommunicationsJune 17, 2026Yuhan Zhou, Jing Li, Fangfang Liu, Yuan Gao, Songlin Yin, Liguo Yang, Xiaoxiao Li, Junhong Lin, Yan Li, Haotian Shang, et al.Cells were washed, fixed and permeabilized by Fixation Buffer (420801, Biolegend) and Perm/Wash Buffer (421002, Biolegend), respectively. Then, cells were stained with anti-IFNγ (XMG1.2, Biolegend) and anti-TNFα

(17) Case Report: Immune checkpoint inhibitor-associated myocarditis, myositis, and myasthenia gravis overlap syndrome with flow cytometric phenotyping before and after treatment in a patient with urothelial carcinomaFrontiers in ImmunologyJune 15, 2026Karen Gambina, Carly Tymm, Matthieu Paiola, Kurenai Tanji, John Zech, Robert Winchester, Adam Mor, Yevgeniya GartshteynFixation Buffer (BioLegend #420801) and treated with Intracellular Staining Fixation Buffer (BioLegend 420801 and 421002) before intracellular staining for granzyme B and SAP. Spectral flow data was obtained on the Cytek

(18) The Effects of CB2R Activation on Inflammatory Pathways in DermatomyositisBiomedicinesJune 7, 2026Rohan Dhiman, Ahmed Eldaboush, Navin Vijayarangan, Darae Kang, Nilesh Kodali, DeAnna Diaz, Caroline Stone, Rui Feng, Victoria P. Werth(Biolegend USA, #420801). This was then followed by resuspension in permeabilization buffer (Biolegend USA, #421002). The following antibodies were added to all tubes: PE CD68 (Biolegend USA, #333808) and BUV 661 CD163

(19) Effect of the JAK Inhibitor Baricitinib on Cytokine Production and Bone Properties in a Mouse Model of Accelerated AgingInt J Mol SciJune 3, 2026Katharina Gelles, Vincent Kurz, Maria Butylina, Katharina Wahl-Figlash, Martin Schepelmann, Anastasia Meshcheryakova, Peter Pietschmannby washing twice with Perm/Wash (Intracellular Staining Permeabilization Wash Buffer 10×; BioLegend, 421002). Meanwhile, different combinations of antibodies were prepared in Perm/Wash. Cells were resuspended

(20) NMI Regulates Adipose Adaptive Thermogenesis Through TLR4/IRF3 Signaling to Promote ObesityAdvanced ScienceJune 2, 2026Ting‐Ting Li, Xin‐Yuan Zhao, Min Zhang, Zhuang‐Feng Weng, Xiao‐Ran Guo, Ying‐Fang Liu, Qiao‐Ping Wang, Huan‐Huan Liangincubation with antibodies diluted in intracellular permeabilization wash buffer (Biolegend, USA, Cat#421002). The following antibodies were used: FITC‐conjugated anti‐mouse CD45 (Cat#157214) and PerCP/Cy5.5‐conjugated

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2/13/2020Easy-to-Use Intracellular Staining Permeabilization Wash Buffer Sunita MeenaOverall best product.

How to maximize your Treg research: from fixation/permeabilization buffers to Nanobodies
March 31, 2015
In defending the human body from invading pathogens, the immune system has to walk a fine line between reacting to foreign antigens and over-reacting to foreign antigens. To help modulate this response the immune system... read more

Intracellular Staining Perm Wash Buffer (10X) from BioLegend

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How to maximize your Treg research: from fixation/permeabilization buffers to Nanobodies

Intracellular Staining Perm Wash Buffer (10X) from BioLegend

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