Human TruStain FcX™ from BioLegend

Product Specs
ItemHuman TruStain FcX™
CompanyBioLegend
Price $229.00Supplier Page View Company Product Page
Catalog Number422302
Quantity200T
ApplicationsFC,ICC,ICFC

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Systemic delivery of phagocytosis-shielded retroviral vectors enables in vivo HSC gene therapy for sickle cell diseaseCell Stem CellSeptember 24, 2026Denise Klatt, Adele Mucci, Boya Liu, Marie Peschers, Giulia Schiroli, Axel Schambach, John Manis, Myriam Armant, David T. Scadden, Els Verhoeyen, et al.,Sigma Aldrich,Cat# 213519–1G Counting Beads,Thermo Fisher Scientific,Cat# C36950 Human TruStain FcX,BioLegend,Cat# 422302 Mouse TruStain FcX,BioLegend,Cat# 101320 Zombie Aqua Viability Dye,BioLegend,Cat# 423102 Critical

(2) Selective Expansion of TCR Vβ2+ Tregs and Impaired Lymphocyte TSST-1 Responsiveness in Bullous Pemphigoid Patients Colonised by Staphylococcus aureusExp DermatolSeptember 21, 2026Tian Y. Chen, Tyler P. Crowe, Maryam Fakhimi, Mia Poleksíc, Samuel H. Kilgore, Patrick M. Schlievert, Janet A. Fairley, Kelly N. Messingham540 viability dye (Tonbo Biosciences Cat#13–0879‐T100, San Diego, CA), Fc block (FcX, BioLegend Cat# 422302, RRID:AB_2818986) and CD3‐Alexa Fluor700 (BioLegend Cat# 317340, RRID:AB_2563408), CD4‐Alexa Fluor488

(3) Targeting TGFBR1 with the Investigational Inhibitor TP-6379 Improves Hematopoiesis in Low-Risk MDS Ex VivoCellsSeptember 16, 2026Tiffany Razabdouski, Robert Dalton, Alexandra Calescibetta, Sean Christiansen, Grace Ward, Anthony C. Augello, Jungwon Moon, Seongseok Yun, Nicole Vincellette, Gabriela Wright, et al.Thermo Fisher Scientific, Waltham, MA, USA). To block Fc receptors, we used the TrueStain FcX block (Cat# 422302, Biolegend, San Diego, CA, USA) at room temperature for 15 min. Cells were subsequently stained with

(4) TLR7/9 and type I interferon signaling reprogram neutrophil NET responses and impair antibacterial defense in systemic lupus erythematosusCell Press BlueSeptember 16, 2026Eden G. TenBarge, Ashley D. Wise, Morgan L. Hetzel, Helene A. Hoover, Helia Esfandiari, Bailey E. Holder, Eva Belevska, Ellie C. Mennen, Sarah R. McDaniel, Nicole M. Vaccaro, et al.AB_940411 PE isotype control,Biolegend,400508 α-CD11b,Biolegend,60-0112-U100 Human Trustain FcX,Biolegend,422302-BL anti-Ly6G,Biolegend,127643; RRID: AB_2565971 anti-SOD2,Cell Signaling,D3X8F anti-LDHB,Invitrogen,MA541126;

(5) Protocol for robust gene knockout and reliable validation in human cell lines using quad-guide RNA vectorsSTAR ProtocSeptember 16, 2026Michael Tobin, Jie Dai, Li-Chuan Chan, Weiwei GuoPrimer3,N/A OligoAnalyzer,Integrated DNA Technologies,N/A ,, Other,Other,Other ,, Human TruStain FcX,Biolegend,Cat#: 422302 Live/Dead Fixable Blue Dead Cell Stain Kit,Invitrogen,Cat#: L23105 96 well clear ultra-low attachment

(6) Development and validation of a long-term co-maturation protocol for human stem cell-derived microglia and neuronal networksStem Cell ReportsSeptember 8, 2026Annika Mordelt, Imke M.E. Schuurmans, Nicky Scheefhals, Marina P. Hommersom, Koen Slottje, Kimberly Mast, Mara Graziani, Carlos O. González, Klaas W. Mulder, Laura J.A. Wingens, et al.to Fc-receptors, the cells were blocked with 2.5% Human TruStain FcX blocking solution (BioLegend, 422302) for 10 min. Following antibodies were used: CD43-PE (1:100, BioLegend, 343203), CD45-FITC (1:200, eBioscience,

(7) Exploring the functions of NKG7 in CD4+ and CD8+ T cells during malariaPLoS PathogSeptember 8, 2026Teija C-M. Frame, Fabian de Labastida Rivera, Nicholas L. Dooley, Jessica A. Engel, Jinrui Na, Yulin Wang, Luzia Bukali, Susanna S. Ng, Patrick T. Bunn, Lynette Beattie, et al.REAGENT or RESOURCE,SOURCE,IDENTIFIER Human TruStain FcX,BioLegend,Cat#422302 TruStain FcX (α-mouseCD16/32) Antibody (clone: 93),BioLegend,Cat#101320 TruStain FcX PLUS α-mouse

(8) Regulators of interferon-responsive microglia uncovered by Genome-wide CRISPRi screeningNPJ DementSeptember 2, 2026Amanda McQuade, Vincent Cele Castillo, Venus Hagan, Weiwei Liang, Thomas Ta, Reet Mishra, Olivia Teter, Layla Gomes, Bianca Gonzalez, Noam Teyssier, et al.(CD38, CD9, P2RY12), cells were incubated with antibodies for 30 min at 4 °C (FC block, Biolegend, 422302, 1:200; anti-CD38, R&D Systems, FAB2404P, 1:200; anti-CD9, Biolegend, 312104, 1:200; anti-P2RY12, Biolegend,

(9) A skin-kidney neutrophil axis links ultraviolet exposure to renal angiogenesis and tissue remodeling in healthy and lupus kidneysJ ImmunolSeptember 1, 2026Angelique Cortez, Lindsay K Mendyka, Alecia Roy, Joshua J Skydel, Lais Osmani, Paola Garcia, Elizabeth C Nowak, Akshaya Balasubramanian, Noelle N Kosarek, Fred W Kolling, et al.Live/Dead blue viability dye (Thermo Fisher Scientific) and treated with Fc Block TruStain FcX (BioLegend; 422302) followed by staining with mouse-specific antibody cocktail (Table S2). Flow cytometry was performed

(10) Impact of Proton versus Photon (Chemo)radiation on Circulating Immune Cells in Head and Neck CancerCancer Res CommunSeptember 1, 2026Anneke L. Eerkens, Nienke van Rooij, Annechien Plat, Annegé Vledder, Koen Brummel, Sjoukje F. Oosting, Bert van der Vegt, Gyorgy B. Halmos, Johannes A. Langendijk, Marco de Bruyn, et al.cells were blocked by incubation with Human TruStain FcX receptor (10 μL per sample; BioLegend, cat. #422302, RRID: AB_281898) and CellBlox blocking buffer (5 μL per sample; Thermo Fisher Scientific). Next, cells

(11) A distinct effector B cell population drives autoantibody production in SARS-CoV-2 infectionImmunityAugust 28, 2026Dan Yuan, Sarah Li, Rongyu Zhang, Rachel H. Ng, Yapeng Su, Linda Lan, Mahasish Shome, Brett Smith, Pamela Troisch, Jingyi Xie, et al.B,Diarect,Cat#A12500 SmD,Diarect,Cat#A11700 Scl-70,Diarect,Cat#A14500 Fc Receptor Blocking Solution,Biolegend,Cat#422302 Monocyte Blocker,Biolegend,Cat#426102 FBS for B cell culture,Cytiva,Cat#SH30070.03 β-mercaptoethanol,Invitrogen,Cat#21985023 HEPES,Invitrogen,Cat#15630080 Sodium

(12) Modeling Risk Group 4 virus infection and antiviral treatment in microfluidic lung organ-on-chips in maximum containment laboratoriesbioRxivAugust 27, 2026Sushma M. Bhosle, Julie P. Tran, Shuiqing Yu, Jillian Geiger, Arpita Das, Scott M. Anthony, Bapi Pahar, Rebecca Bernbaum-Cutler, Deja F. P. Rivera, Ian Crozier, et al.30 μL per mL of whole blood of Human TruStain FcX (Fc Receptor Blocking Solution) (BioLegend; Cat. #422302) and incubated in the dark for 10 min on ice. Next, a surface-stain master mix (30 μL of PTPRC: APC

(13) Pre-existing systemic immune state and dynamic myeloid remodeling shape response to neoadjuvant PD-L1 blockadeJournal for ImmunoTherapy of CancerAugust 26, 2026Stefan Kasper, Hendrik Winkel, Benedikt Höing, Kirsten Bruderek, Michael Pogorzelski, Eric Deuss, Inga Grünewald, Christian Doreth, Xi Wang, Sarah Theurer, et al.barcoded samples were incubated in 250 µL of Fc blocking solution (25 µL of TruFcX (BioLegend cat# 422302) in 225 µL of cell staining media) for 10 min at room temperature with agitation. Surface antibody cocktail

(14) Ficoll-based isolation biases the profiling of immune cells and leukemic blasts in AML samplesFrontiers in ImmunologyAugust 25, 2026Bianca E Silva, Alison Daubry, Charline Faville, Adrien De Voeght, Jacques Foguenne, Mégane Jassin, Oswin Kwan, Leslie Correia Da Cruz, Gabriele Carriglio, Sébastien Charles, et al.for flow cytometry staining. Cells were incubated with Fc receptor blocking solution (Human TruStain FcX, cat: 422302; Biolegend) for 10 minutes at room temperature before overnight incubation with surface antibodies at

(15) The immunophenotype and proviral landscape of HIV-infected CD4 T cells during antiretroviral therapyProc Natl Acad Sci U S AAugust 25, 2026Cyrille L. Delley, Sakshi Shah, Kevin M. Joslin, Yujung P. Park, Benjamin Demaree, Michael P. Busch, Mars Stone, Steven G. Deeks, Eli A. Boritz, Adam R. Abate, et al.Oligonucleotide-Conjugated Antibodies. 10 μL Human TruStain FcX Fc Receptor Blocking Solution (BioLegend 422302) and 4 μL UltraPure Salmon Sperm DNA Solution (ThermoFisher 15632011) were added to cells in PBS-F to

(16) XPO1-mediated TRIM21 nuclear export reprograms TREM2+ macrophage polarization by targeting IRF3 to augment anti-PD-1 efficacy in small cell lung cancerNature CommunicationsAugust 22, 2026Qingwu Du, Tingting Qin, Jingya Wang, Yantao Jiang, Qi Xu, Xueyang Li, Huiyan Liu, Junjie Yu, Yi Lu, Luyao Tong, et al.chamber of Transwell inserts and Fc receptors were blocked using Human TruStain FcX (BioLegend, Cat. 422302) for 10 min at 4 °C. Cells were then incubated with anti-human TREM2 antibody (R&D Systems, Cat. FAB17291A)

(17) Six-compound cocktail for maturation of human pluripotent stem cell-derived liver spheroids for toxicity predictionNature CommunicationsAugust 21, 2026Lipeng Tian, Faiza H. Anas, Ryan Hasselkus, Elsa Joseph, Michelle Chadraa, Baran Koç, Wei Wu, Bhavana Karnam, Pablo Flores-Muñoz, Ariel Alon, et al.After washing, cells were blocked with TruStain FcX™ Fc receptor blocking reagent (BioLegend, Cat. #422302) for 10 min at 4 °C. Without additional washing, BV785-conjugated antibodies against CD14 (Biolegend,

(18) Safety and immunogenicity of an adjuvant-free peptide vaccine targeting IL-17A: Phase 1 randomized controlled trialiScienceAugust 21, 2026Satoru Kasahara, Akiko Tenma, Makoto Sakaguchi, Shigeyoshi Tsuji, Hideki Tomioka, Ryuichi Morishita, Hironori Nakagami, Tetsuya Tomitapreabsorbed,Abcam,Cat# Ab97175; RRID: AB_10680841 "Human TruStain FcX™(Fc Receptor Blocking Solution), BioLegend, 422302",BioLegend,Cat# 422302; RRID:AB_2818986 Brilliant Violet 510™ anti-human CD3 Antibody,BioLegend,Cat#

(19) Anti-CD6 monoclonal antibody UMCD6 induces CD6 internalization, remodels costimulatory receptor availability, and modulates CD4+ T-cell activationFrontiers in ImmunologyAugust 19, 2026Kohei Maeda, Phillip L. Campbell, Qi Wu, Pei-Suen Tsou, Laura A. Cooney, Yang Mao-Draayer, David A. Fox, Mikel Gurrea-Rubiobuffer (BioLegend, Cat# 420201), and treated with FcR blocker (Human TruStain FcX, BioLegend, Cat# 422302) and fixable viability dye (FVD) (Zombie Yellow™ Fixable Viability Kit, BioLegend, Cat# 423103) for

(20) Molecular basis for protection and cross-protection by human antibodies targeting the parainfluenza virus hemagglutinin-neuraminidase proteinJournal of VirologyAugust 18, 2026Katelyn D. McCaffrey, Behrouz Ghazi Esfahani, Mohamed A. Elbehairy, Anna L. McCormick, Jarrod J. Mousa5217-9 mAb discovery PBMCs from blood of human donors were blocked with Trustain FcX (Biolegend, Cat: 422302) for 30 min, washed once in PBS, and subsequently stained according to the following protocol: 5 µL

Write a Review

(1)

10/6/2023Great Reagent From Biolegend for Flow Blocking Danielle SchmidtWe use this block during flow cytometry before adding our panel antibodies. It does a great job blocking common proteins that we are not trying to measure
(1)

8/11/2023Great Fc Block From BioLegend Lana JagannathanI use this Fc block for a variety of staining purposes, mainly to stain monocyte-derived dendritic cells for flow cytometry. It has never let me down!
(1)

9/23/2022Reliable Fc Block for Flow Cytometry of Human Cells Vincent GiacaloneThis reagent is used to block Fc receptors on cells for flow cytometry staining to prevent non-specific binding of antibodies.
(1)

7/30/2020User Friendly Fc Receptor Blocker Rebecca GuyerGood Fc blocker to add to your antibody cocktails when staining for flow cytometry

Human TruStain FcX™ from BioLegend

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Human TruStain FcX™ from BioLegend

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