Human TruStain FcX™ from BioLegend

Product Specs
ItemHuman TruStain FcX™
CompanyBioLegend
Price $229.00Supplier Page View Company Product Page
Catalog Number422302
Quantity200T
ApplicationsFC,ICC,ICFC

Add to Compare List

BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) The immunophenotype and proviral landscape of HIV-infected CD4 T cells during antiretroviral therapyProc Natl Acad Sci U S AAugust 25, 2026Cyrille L. Delley, Sakshi Shah, Kevin M. Joslin, Yujung P. Park, Benjamin Demaree, Michael P. Busch, Mars Stone, Steven G. Deeks, Eli A. Boritz, Adam R. Abate, et al.Oligonucleotide-Conjugated Antibodies. 10 μL Human TruStain FcX Fc Receptor Blocking Solution (BioLegend 422302) and 4 μL UltraPure Salmon Sperm DNA Solution (ThermoFisher 15632011) were added to cells in PBS-F to

(2) Safety and immunogenicity of an adjuvant-free peptide vaccine targeting IL-17A: Phase 1 randomized controlled trialiScienceAugust 21, 2026Satoru Kasahara, Akiko Tenma, Makoto Sakaguchi, Shigeyoshi Tsuji, Hideki Tomioka, Ryuichi Morishita, Hironori Nakagami, Tetsuya Tomitapreabsorbed,Abcam,Cat# Ab97175; RRID: AB_10680841 "Human TruStain FcX™(Fc Receptor Blocking Solution), BioLegend, 422302",BioLegend,Cat# 422302; RRID:AB_2818986 Brilliant Violet 510™ anti-human CD3 Antibody,BioLegend,Cat#

(3) Köhlmeier-Degos disease is an interferonopathy characterized by type I and II interferon-driven inflammatory vasculopathyCell Reports MedicineAugust 18, 2026Cornelia D. Cudrici, Shubham Goel, Keiko Sakamoto, Seon-Pil Jin, Akiko Sekiguchi, Douglas R. Rosing, Rebecca Huffstutler, Dima A. Hammoud, Lee Shapiro, Daniella M. Schwartz, et al.Antibody,Biolegend,Cat# 302270; RRID:AB_2832581 Human TruStain FcX™ (Fc Receptor Blocking Solution),Biolegend,Cat# 422302; RRID:AB_2818986 Zombie Violet™ Fixable Viability Kit,Biolegend,Cat# 423114 Rabbit anti-human CD4

(4) Molecular basis for protection and cross-protection by human antibodies targeting the parainfluenza virus hemagglutinin-neuraminidase proteinJournal of VirologyAugust 18, 2026Katelyn D. McCaffrey, Behrouz Ghazi Esfahani, Mohamed A. Elbehairy, Anna L. McCormick, Jarrod J. Mousa5217-9 mAb discovery PBMCs from blood of human donors were blocked with Trustain FcX (Biolegend, Cat: 422302) for 30 min, washed once in PBS, and subsequently stained according to the following protocol: 5 µL

(5) CD6-Directed Immunotherapy Targets Breast Cancer Stem Cell Function and Enhances Immune-Mediated Cytotoxicity in Triple-Negative Breast CancerBiomoleculesAugust 17, 2026Mikel Gurrea-Rubio, Sophie Sloan, Aditya Chada, Camila I. Amarista, Kohei Maeda, Phillip L. Campbell, Pei-Suen Tsou, Laura A. Cooney, Max S. Wicha, David A. Fox(BioLegend, Cat# 420201), cells were incubated with Human TruStain FcX™ Fc receptor blocker (BioLegend, Cat# 422302) for 10 min at room temperature to prevent nonspecific binding. Cells were subsequently stained with

(6) Induced pluripotent stem cell-derived macrophages enable broad modeling of human inflammasome signalingCell Reports MethodsAugust 17, 2026Chloe M. McKee, Melanie Cranston, Emma C. McKay, Mohammad Arefian, Thea J. Mawhinney, Ben C. Collins, Rebecca C. Collroom temperature then washed twice in DPBS. Blocking solution (Human TruStain FXX, Biolegend, Cat # 422302) was added at a dilution of 1:20 for 30 min in the dark at room temperature and cells were washed twice

(7) Precision tumor-on-chip for personalized assessment of drug efficacy and immune cell deliveryCell Reports MethodsAugust 17, 2026Elena Kremneva, Johannes Smolander, Mimosa Peltokangas, Mikaela Grönholm, Lilja Lahtinen, Shadi Jansouz, Sanna Vainionpää, Bassel Alsaed, Kristian Borenius, Anniina Palojärvi, et al.blocked for 10 min on ice in the presence of TruStain FcX blocking reagent (FcX, BioLegend, cat. # 422302). A unique TotalSeq-A anti-human hashing antibody (TotalSeq-A0251, TotalSeq-A0252, TotalSeq-A0253, TotalSeq-A0254,

(8) Comparison of THP-1 Subtypes Reveals Differences on Transcriptomic and Protein Expression Level Throughout Their Immunological DifferentiationJ Immunol ResAugust 17, 2026Sabine Klatt, Gudrun Marquardt, Miriam Faxel, Stefan Rubner, Ioannis Papasotirioucell staining buffer (Biolegend, 420201). Nonspecific binding was blocked using human TruStain (Biolegend, 422302) for 15 min. Cells were stained with respective antibodies (including isotype controls) for 30 min (Table

(9) Lactate-Induced K370 Lactylation of STING Inhibits STING-TBK1 Signaling and Dampens Anti-Tumor ImmunityAdv Sci (Weinh)August 15, 2026Yueyao Wu, Jingzhe Wang, Xu Chen, Yuntong Yang, Ping Wei, Han Xie, Honghao Wang, Chunyu Zhang, Siyi Xu, Qi Wang, et al.cell strainer. Following cell counting, Fc receptors were blocked with Human TruStain FcX (BioLegend, 422302) for 10 min. Cells were then stained with fluorophore‐conjugated antibodies for 20 min at room‐temperature

(10) Novel therapeutic strategies for ccRCC through integration of systemic drug combinations and oncolytic adenovirus immunotherapyFront ImmunolAugust 14, 2026Michaela Feodoroff, Isabel Mogollon, Firas Hamdan, Tamara J. Luck, Romika Kumari, Paolo Bottega, Janita Sandberg, Erika Romppanen, Lassi Paavolainen, Minna Malmstedt, et al.CAT#304604), PerCP anti-human CD107a (Clone H4A3, BioLegend, CAT# 328641), Human TruStain FcX (BioLegend Cat#422302). PDCs were plated at 1 × 104 cells/well on 96-well plates and exposed to AdCab virus at a multiplicity

(11) Rituximab Binding Endows CD20+ Extracellular Vesicles With NK Cell-Activating Properties in B Cell LymphomaJ Extracell VesiclesAugust 9, 2026Adrián V. Otero, Paula Soledad Pérez, Belen Gaete-Ramírez, Gregorio Cordini, Matías Norte, Enriqueta Martínez, Nicolás Marsol, Cecilia Malusardi, Sofía Rivarola, Ana Cantillo, et al.by flow cytometry. In some experiments NK cells were previously exposed to Fc‐Blocking (Biolegend, #422302). Target cells alone and/or cells exposed to EVs were used as controls. When cytotoxic effect on Ramos

(12) B cell CD19 is transferred between immune cells in mice and humansNat CommunJuly 29, 2026Jasmin Ochs, Pia Schweineberg, Jacqueline Thode, Alica Blenkle, Leila Husseini, Matthias Klein, Tobias Bopp, Patrick Schindler, Friedemann Paul, Martin S. Webermonoclonal antibody specific for CD16/CD32 (Human TruStain FcX (Fc Receptor Blocking Solution); BioLegend; #422302). Dead human and murine cells were stained with LIVE/DEAD Fixable NIR Dead Cell Stain Kit (Thermo Fisher

(13) Immunologic determinants of infusion products and the tumor microenvironment govern response to TIL therapy in advanced melanomaMedJuly 29, 2026Lilit Karapetyan, Jin Xu, Kimberly Ward, Denise Kalos, Benjamin Schachner, Johannes Ali, Xiaofei Song, Joel Kuriakose, MacLean S. Hall, Jamie Chau, et al.Biotechnologies,Commercial assay Zombie NIR Fixable Viability Kit,BioLegend,Cat# 423105 Human TruStain FcX,BioLegend,Cat# 422302 Brilliant Stain Buffer Plus,BD Biosciences,Cat# 566385 FoxP3/Transcription Factor Staining Buffer

(14) Transcriptional regulation of disease-relevant microglial activation programsNeuronJuly 29, 2026Amanda McQuade, Reet Mishra, Venus Hagan, Weiwei Liang, Peter J. Colias, Vincent Cele Castillo, Bianca Gonzalez, Justin P. Lubin, Verena Haage, Victoria Marshe, et al.anti-human CD45 2D1 APC/Cy7,Biolegend,Cat #368515; RRID: AB_2566375 Human TruStain FcX,Biolegend,Cat#422302; RRID:AB_2818986 Rabbit anti-human PU.1,Cell Signaling,Cat #2266S; RRID: AB_10692379 "anti-human CD86

(15) Protocol to study inflammasome activation in induced pluripotent stem cell-derived macrophagesSTAR ProtocJuly 28, 2026Chloe M. McKee, Melanie Cranston, Emma C. McKay, Mohammad Arefian, Ben C. Collins, Rebecca C. CollTalabostat mesylate,R&D Systems,3719/10 Anisomycin from Streptomyces griseolus,Merck,A9789 Human TruStain FXX,Biolegend,422302 eBioscience™ Intracellular Fixation & Permeabilization Buffer Set,ThermoFisher,88-8824-00 OptiMEM,ThermoFisher,31985070 "Latex

(16) Viscoelastic Niches Shape γδ T-Cell Phenotype and Effector FunctionbioRxivJuly 24, 2026Favour Omafuvwe Obuseh, Michelle Chang, Joshua Price, Kyle Ruark, Tania To, Leah Lourenco, Bogdan Budnik, David Mooneymanufacturer’s protocol. Cells were then blocked with FcX Fc receptor blocking solution (Biolegend, 422302) for 20 min and stained with surface protein antibodies for 30 min. Brilliant violet staining buffer

(17) A Microgel-Based Platform for Tunable Expansion and Function of γδ T-cellsbioRxivJuly 24, 2026Favour Omafuvwe Obuseh, Junzhe Lou, Michelle Chang, Anqi Chen, David Weitz, David Mooneymanufacturer’s protocol. Cells were then blocked with FcX Fc receptor blocking solution (Biolegend, 422302) for 20 min and stained with surface protein antibodies for 30 min. Brilliant violet staining buffer

(18) Model-dependent GD2 upregulation in Ewing sarcoma with the EZH2 inhibitor tazemetostat: Prerequisites for combination with GD2-specific CAR T cellsResearchSquareJuly 24, 2026Lara Bücker, Bianca Altvater, Jutta Meltzer, Nicole Farwick, Karen E Pollok, Pankita H Pandya, Mohammad R Saadatzadeh, Ramona Meissner, Wolfgang Hartmann, Claudia Rossig, et al.block non-specific binding and then for 5 min with Human TruStain FcXTM (Fc Receptor Blocking Solution, 422302 Biolegend, Germany) to block unwanted staining involving FcR. Cells were then incubated with the following

(19) Intrinsic tumor cell line immunogenicity may drive CAR-independent T cell responses and confound CAR T cell preclinical modelingJ Immunother CancerJuly 23, 2026Michael K Sheng, Wahed A Firoz, Kelly Loi, Logan V Vick, Craig P Collins, Ryan N Nielsen, Jessica L Schmeling, Shuchi Gulati, Sean J Judge, Robert J Canter, et al.Zombie NIR viability dye, Fc blocked with a 1:20 dilution of human TruStain FcX (BioLegend, Cat. #: 422302), surface stained (FITC anti-human CD19, PE anti-human HLA-A, B, C (BioLegend, Cat. #: 311406), APC anti-human HLA-DR, DP, DQ

(20) Oncolytic adenovirus armed with cGAS activates STING pathway and enhances antitumor immunity in lung cancer with superior combined efficacy of PD-L1 therapyFront ImmunolJuly 23, 2026Qing-Wen Wang, Hua-Wei Xu, Yu-Sen Shi, Yi-Peng Zhang, Jie Jun, Dan-Ning Yue, Wei Zhao, Jia-Qiang Huang, Xiang-Lei Peng, Jie-Mei Yu, et al.system were transferred into U-bottom 96-well plates and blocked with Human TruStain FcX™ (BioLegend, 422302, USA). CD8-, CD56-, and CD69-conjugated fluorescent antibodies were added, and cells were incubated

Write a Review

(1)

10/6/2023Great Reagent From Biolegend for Flow Blocking Danielle SchmidtWe use this block during flow cytometry before adding our panel antibodies. It does a great job blocking common proteins that we are not trying to measure
(1)

8/11/2023Great Fc Block From BioLegend Lana JagannathanI use this Fc block for a variety of staining purposes, mainly to stain monocyte-derived dendritic cells for flow cytometry. It has never let me down!
(1)

9/23/2022Reliable Fc Block for Flow Cytometry of Human Cells Vincent GiacaloneThis reagent is used to block Fc receptors on cells for flow cytometry staining to prevent non-specific binding of antibodies.
(1)

7/30/2020User Friendly Fc Receptor Blocker Rebecca GuyerGood Fc blocker to add to your antibody cocktails when staining for flow cytometry

Human TruStain FcX™ from BioLegend

Description

BioLegend

Citations (1314)

Reviews

Human TruStain FcX™ from BioLegend

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map