Human TruStain FcX™ from BioLegend

Product Specs
ItemHuman TruStain FcX™
CompanyBioLegend
Price $229.00Supplier Page View Company Product Page
Catalog Number422302
Quantity200 tests
ApplicationsFC - Quality tested, ICFC, ICC - Reported in the literature, not verified in house

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Safety and immunogenicity of an adjuvant-free peptide vaccine targeting IL-17A: Phase 1 randomized controlled trialiScienceAugust 21, 2026Satoru Kasahara, Akiko Tenma, Makoto Sakaguchi, Shigeyoshi Tsuji, Hideki Tomioka, Ryuichi Morishita, Hironori Nakagami, Tetsuya Tomitapreabsorbed,Abcam,Cat# Ab97175; RRID: AB_10680841 "Human TruStain FcX™(Fc Receptor Blocking Solution), BioLegend, 422302",BioLegend,Cat# 422302; RRID:AB_2818986 Brilliant Violet 510™ anti-human CD3 Antibody,BioLegend,Cat#

(2) B cell CD19 is transferred between immune cells in mice and humansNat CommunJuly 29, 2026Jasmin Ochs, Pia Schweineberg, Jacqueline Thode, Alica Blenkle, Leila Husseini, Matthias Klein, Tobias Bopp, Patrick Schindler, Friedemann Paul, Martin S. Webermonoclonal antibody specific for CD16/CD32 (Human TruStain FcX (Fc Receptor Blocking Solution); BioLegend; #422302). Dead human and murine cells were stained with LIVE/DEAD Fixable NIR Dead Cell Stain Kit (Thermo Fisher

(3) Immunologic determinants of infusion products and the tumor microenvironment govern response to TIL therapy in advanced melanomaMedJuly 29, 2026Lilit Karapetyan, Jin Xu, Kimberly Ward, Denise Kalos, Benjamin Schachner, Johannes Ali, Xiaofei Song, Joel Kuriakose, MacLean S. Hall, Jamie Chau, et al.Biotechnologies,Commercial assay Zombie NIR Fixable Viability Kit,BioLegend,Cat# 423105 Human TruStain FcX,BioLegend,Cat# 422302 Brilliant Stain Buffer Plus,BD Biosciences,Cat# 566385 FoxP3/Transcription Factor Staining Buffer

(4) Protocol to study inflammasome activation in induced pluripotent stem cell-derived macrophagesSTAR ProtocJuly 28, 2026Chloe M. McKee, Melanie Cranston, Emma C. McKay, Mohammad Arefian, Ben C. Collins, Rebecca C. CollTalabostat mesylate,R&D Systems,3719/10 Anisomycin from Streptomyces griseolus,Merck,A9789 Human TruStain FXX,Biolegend,422302 eBioscience™ Intracellular Fixation & Permeabilization Buffer Set,ThermoFisher,88-8824-00 OptiMEM,ThermoFisher,31985070 "Latex

(5) A Microgel-Based Platform for Tunable Expansion and Function of γδ T-cellsbioRxivJuly 24, 2026Favour Omafuvwe Obuseh, Junzhe Lou, Michelle Chang, Anqi Chen, David Weitz, David Mooneymanufacturer’s protocol. Cells were then blocked with FcX Fc receptor blocking solution (Biolegend, 422302) for 20 min and stained with surface protein antibodies for 30 min. Brilliant violet staining buffer

(6) Model-dependent GD2 upregulation in Ewing sarcoma with the EZH2 inhibitor tazemetostat: Prerequisites for combination with GD2-specific CAR T cellsResearchSquareJuly 24, 2026Lara Bücker, Bianca Altvater, Jutta Meltzer, Nicole Farwick, Karen E Pollok, Pankita H Pandya, Mohammad R Saadatzadeh, Ramona Meissner, Wolfgang Hartmann, Claudia Rossig, et al.block non-specific binding and then for 5 min with Human TruStain FcXTM (Fc Receptor Blocking Solution, 422302 Biolegend, Germany) to block unwanted staining involving FcR. Cells were then incubated with the following

(7) Viscoelastic Niches Shape γδ T-Cell Phenotype and Effector FunctionbioRxivJuly 24, 2026Favour Omafuvwe Obuseh, Michelle Chang, Joshua Price, Kyle Ruark, Tania To, Leah Lourenco, Bogdan Budnik, David Mooneymanufacturer’s protocol. Cells were then blocked with FcX Fc receptor blocking solution (Biolegend, 422302) for 20 min and stained with surface protein antibodies for 30 min. Brilliant violet staining buffer

(8) Oncolytic adenovirus armed with cGAS activates STING pathway and enhances antitumor immunity in lung cancer with superior combined efficacy of PD-L1 therapyFront ImmunolJuly 23, 2026Qing-Wen Wang, Hua-Wei Xu, Yu-Sen Shi, Yi-Peng Zhang, Jie Jun, Dan-Ning Yue, Wei Zhao, Jia-Qiang Huang, Xiang-Lei Peng, Jie-Mei Yu, et al.system were transferred into U-bottom 96-well plates and blocked with Human TruStain FcX™ (BioLegend, 422302, USA). CD8-, CD56-, and CD69-conjugated fluorescent antibodies were added, and cells were incubated

(9) Intrinsic tumor cell line immunogenicity may drive CAR-independent T cell responses and confound CAR T cell preclinical modelingJ Immunother CancerJuly 23, 2026Michael K Sheng, Wahed A Firoz, Kelly Loi, Logan V Vick, Craig P Collins, Ryan N Nielsen, Jessica L Schmeling, Shuchi Gulati, Sean J Judge, Robert J Canter, et al.Zombie NIR viability dye, Fc blocked with a 1:20 dilution of human TruStain FcX (BioLegend, Cat. #: 422302), surface stained (FITC anti-human CD19, PE anti-human HLA-A, B, C (BioLegend, Cat. #: 311406), APC anti-human HLA-DR, DP, DQ

(10) iPSC-Derived Microglia-like Cells Exhibit Protocol-Dependent Transcriptomic Features and Robust Phagocytosis of Glioma CellsbioRxivJuly 22, 2026Maya N. Walker, Hui Tang, Caylee Silvers, Sasha Roth, Deanna Tiek, Bo Hu, Shi-Yuan Cheng, Xiao Songwere harvested and stained with Human TruStain FcX™ Fc receptor blocking solution (BioLegend, Cat. No. 422302), followed by fluorophore-conjugated antibodies against human CD45-FITC (BioLegend, Cat. No. 304006),

(11) Navigating Human Astrocyte Differentiation: Direct and Rapid One-Step Differentiation of Induced Pluripotent Stem Cells to Functional Astrocytes Supporting Neuronal Network DevelopmentGliaJuly 15, 2026Imke M. E. Schuurmans, Annika Mordelt, Marta Guevara-Ferrer, Katrin Linda, Denise Duineveld, Sofia Puvogel, Marina P. Hommersom, Nicky Scheefhals, Lisa Rahm, Emma Dyke, et al.to Fc‐receptors, the cells were blocked with 2.5% Human TruStain FcX blocking solution (BioLegend; 422302) for 10 min. Subsequently, the cells were incubated with anti‐CD44 Brilliant Violet 421 (Biolegend;

(12) Protocol for deep phenotyping of human antigen-specific T cells across conventional and unconventional subsets by 22-color spectral flow cytometrySTAR ProtocJuly 15, 2026Jiahui Du, Mingzhe Zeng, Ziqian He, Xiang Li, Shuxun Liu, Zhiqing LiFITC (working dilution: 1:50),BioLegend,Cat#331208; RRID:AB_1575108 Human TruStain FcX™,BioLegend,Cat#422302; RRID:AB_2818986 Anti-human CD40 Antibody (working dilution: 1:200),MiltenyiBiotechnology Company,130-094-133 ,, Biological

(13) CD157+ vascular endothelial stem cells represent a conserved subpopulation with an angiogenic gene expression profileStem Cell ReportsJuly 14, 2026Tomohiro Iba, Taku Wakabayashi, Rie Ito, Ai Sugawara, Satoshi Fujimura, Mika Sawane, Kazuaki Yoshioka, Aya Matsui, Jun-ichi Morishige, Naoto Nagata, et al.PE anti-mouse CD201 (EPCR),BioLegend,Cat# 141504; RRID: AB_10895909 Human TruStain FcX (Fc Receptor Blocking Solution),BioLegend,Cat# 422302; RRID: AB_2818986 APC anti-human CD31,BioLegend,Cat# 303116; RRID: AB_1877152 Brilliant Violet 421 anti-human CD45

(14) Harnessing human immune system models to validate NADPH oxidase 1 inhibition as treatment for hepatocellular carcinomaFront PharmacolJuly 14, 2026Zenzi De Vos, Lander Heyerick, Aline Baekelandt, Lara Morren, Anne Hoorens, Hasan Eker, Luís Abreu de Carvalho, Filip Gryspeerdt, Frederik Berrevoet, Hannah Stocks, et al.and were added 1/10 for blood and 1/15 for tissue. All antibodies, FcR blocks (mouse #156604, human #422302), live/dead stain (#423102) and counting beads (#424902) were purchased at Biolegend. Samples were measured

(15) Neutrophil myeloperoxidase as a functional biomarker for RSV severity: implications for in vitro therapeutic screeningNat CommunJuly 13, 2026Machaela Palor, Tereza Masonou, Elisabeth J. Robinson, Wenqing Chen, Samuel Ellis, Laura Buggiotti, Amy I. Jacobs, Thomas Benoist, Paolo De Coppi, Jennifer L. Rohn, et al.neutrophils (apical, adherent and basolateral) were collected, centrifuged, blocked with Human TruStain FcX (#422302, BioLegend, USA), and stained with LIVE/DEAD fixable violet dye (#L34963, Thermo Fisher Scientific,

(16) Von Willebrand factor A1 blockade prevents platelet-mediated sustained occlusion for the treatment of arterial thrombosisCommun BiolJuly 11, 2026Emily P. Mihalko, Ronit Kar, Blake Holthaus, Rassam M. G. Rassam, Riya Sharma, Debra Wheeler, Shahid M. Nimjee, Susan M. Sheas⁻¹ within the stenosis region. Whole blood was pre-incubated with human Fc receptor block (Biolegend 422302; 1:600) and anti-CD41:JF646 (Novus Biologicals NB100-2614JF646; 1:1200) unless otherwise noted for 30

(17) CD117 epitope-shielded hematopoietic stem cell transplantation with toxin-free conditioning and in vivo selection ameliorates a β-thalassemia modelbioRxivJuly 8, 2026Romina Marone, Rosalba Lepore, Kiriaki Paschoudi, Jessica Zuin, Alessandro Sinopoli, Anna Camus, Thomas Burgold, Ewelina Bartoszek, Diego Calabrese, Mylène Toranelli, et al.2% FCS +1mM EDTA). Prior to staining, cells were blocked with Human TruStain FcX™ (BioLegend, Cat# 422302) for 15 min at 4 °C. Serial dilutions of SR-1 antibody were prepared to yield final concentrations ranging

(18) Multi-modal single-cell sequencing reveals network transition in circulating monocytes that aligns with faster recovery in patients with trauma and favours a response to M-CSFEBioMedicineJuly 3, 2026Tianmeng Chen, Julia Hughes, Julia Cornoy, Wei Chen, Richard Duerr, Timothy Billiaranalysed using FlowJo v10.0.7. Human TruStain FcX (Fc receptor blocking solution): BioLegend, Cat# 422302, RRID:AB_2818986. Live/Dead Aqua Dead Cell Stain dye: Thermo Fisher Scientific, Cat# L34966. UltraComp

(19) An ICAM1-targeting chimeric costimulatory receptor mimics the immune synapse and enhances tumor-specific T cell functionCancer Immunol ResJuly 2, 2026Irene M. Min, Yanping Yang, Dessislava Stefanova, Yogindra Vedvyas, Diella S. Babu, Dong-Hyun Lee, Yago Alcaina, Maria Cristina Riascos, Janusz Puc, Kevin J. Chen, et al.ammonium-chloride-potassium buffer. After washing, cell pellets from tumor tissues were blocked in TruStain FcX (BioLegend #422302) for 5-10 min before staining. Pellets from cell lines, T cells, and tumor tissues (1-3×105 cells per

(20) Inherited human TFIIIA deficiency disrupts T cell developmentmedRxivJuly 1, 2026Evi Duthoo, Sueun Park, Tamara Jarayseh, Marita Bosticardo, Rafah Mackeh, Yana Van Droogenbroeck, Imke Velghe, Veronique Debacker, Hailun Li, Nourhen Agrebi, et al.2×106 cells were used for staining. Cells were first incubated with FcR blocking reagent (BioLegend; 422302) in PBS, together with a biotin-conjugated antibody and Zombie UV™ Fixable Viability dye (BioLegend;

Write a Review

(1)

10/6/2023Great Reagent From Biolegend for Flow Blocking Danielle SchmidtWe use this block during flow cytometry before adding our panel antibodies. It does a great job blocking common proteins that we are not trying to measure
(1)

8/11/2023Great Fc Block From BioLegend Lana JagannathanI use this Fc block for a variety of staining purposes, mainly to stain monocyte-derived dendritic cells for flow cytometry. It has never let me down!
(1)

9/23/2022Reliable Fc Block for Flow Cytometry of Human Cells Vincent GiacaloneThis reagent is used to block Fc receptors on cells for flow cytometry staining to prevent non-specific binding of antibodies.
(1)

7/30/2020User Friendly Fc Receptor Blocker Rebecca GuyerGood Fc blocker to add to your antibody cocktails when staining for flow cytometry

Human TruStain FcX™ from BioLegend

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Human TruStain FcX™ from BioLegend

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