Human TruStain FcX™ from BioLegend

Product Specs
ItemHuman TruStain FcX™
CompanyBioLegend
Price $229.00Supplier Page View Company Product Page
Catalog Number422302
Quantity200 tests
ApplicationsFC - Quality tested, ICFC, ICC - Reported in the literature, not verified in house

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Distinct CD8+ T-cell Types Associated with COVID-19 Severity in Unvaccinated HLA-A2+ PatientsiScienceJune 19, 2026Kazuya Masuda, Sho Iketani, Lihong Liu, Jing Huang, Yujie Qiao, Jayesh Shah, Meredith L. McNairy, Christine Groso, Christopher L. Ricupero, Lucas F. Loffredo, et al.incubation, the cells were washed 3 times and incubated for 10 minutes with Fc receptor block (BioLegend, 422302). Afterward, each sample was stained with a total of 6 HLA-A2+/peptide fluorescent tetramer (Tet) and

(2) Adenine base editor for knockout of proteins: a practical guide from design to analysis with updated MultiEditRbatchMolecular Therapy Nucleic AcidsJune 16, 2026Ella J. Eaton, Bryce J. Wick, Jeremy S. Chacón, Amy J. Wang, Mitchell Kluesner, Jackson T. Barnes, Bibekananda Kar, Minjing Wang, Matthew J. Johnson, Joseph G. Skeate, et al.were stained using the BD Cytofix/Cytoperm kit (BD Biosciences), human TruStain FcX (BioLegend, Cat #422302), and antibodies listed in Table S3. Flow cytometry was performed on the CytoFlex S (Beckman Coulter)

(3) Protocol for culture, drug treatment, and dissociation of patient-derived 3D ovarian cancer microspheroids for flow cytometrySTAR ProtocolsJune 1, 2026Mariam Haffa, Karolina Juhani, Greta Gudoityte, Rita Hutyra-Gram Ötvös, Emelie Wallin, Olli-Pekka Kallioniemi, Josefin Fernebro, Ulrika Joneborg, Brinton Seashore-LudlowRESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Human TruStain FcX™ (FC Receptor Blocking Solution),BioLegend,Cat#422302; RRID: AB_2818986 "anti-human CD326-APC (EpCAM), clone: 9C4 (1:50 dilution)",BD Bioscience,CAT#566842;

(4) Dual Roles of hABCB1 in Drug Resistance and Immune Evasion: Implications for Lung Cancer TherapyACS OmegaMay 26, 2026Jin Young Min, Hye Min Kim, Geul Bang, Hae Won Jung, Yea Jin Kim, Sang-Yeop Lee, Dong Joon Kim, Sang Keun Ha, Truc Ngoc Kim Lam, Kwan Soo Hong, et al.incubation with an Fc receptor blocker (Human TruStain FcX; BioLegend, San Diego, CA, USA; cat. no. 422302) for 30 min at 4 °C, cells (1 × 105) were stained at 4 °C for 20 min in the dark using the following

(5) Protocol for assessing anti-Siglec-9 antibody-mediated tumor phagocytosis by macrophages using pH-sensitive dyes and flow cytometrySTAR ProtocMay 20, 2026Saitong Muneekaew, Thunchanok Nuchphongsai, Pasut Sasithong, Kritayaporn Saiprayong, Kamonphan Rungrueang, Methichit Wattanapanitch(1:500 dilution),Biolegend,Cat. No. 423102 Human TruStain FcX (TM) (1:20 dilution),BioLegend,Cat. No. 422302 True-Stain Monocyte Blocker (1:20 dilution),BioLegend,Cat. No. 426120 ,, Biological samples,Biological

(6) A Novel PTPN2 Isoform Differentially Regulates Immune ResponseEur J ImmunolMay 19, 2026Xiaoguang Li, Fedik Rahimov, Rui Wang, Lei Gao, Renze Ma, Tiankai Han, Yingtao Bi, Freidrich Cruz, Jing Wang, Shaun Grosskurth, et al.dead cells. Cells were first blocked with Human TruStain FcX (Fc Receptor Blocking Solution) (Catalog# 422302, BioLegend) using a 1:100 dilution and then stained for 30 min at 4°C with staining antibodies. moDCs

(7) An improved preservation method for human dorsal root ganglion neurons enables wider access to human molecular pain neuroscienceCell Reports MethodsMay 18, 2026Joseph B. Lesnak, Mandee K. Schaub, Kimberly Gomez, Aida Calderon-Rivera, Santiago Loya-Lopez, Robert Stewart, Sooyeon Jo, Akie Fujita, Tomás Osorno, Hemanth Mydugolam, et al.RRID: AB_314065 Zombie UV viability dye,BioLegend,423107 TruStain FcX (human Fc block),BioLegend,422302; RRID:AB_28118986 Myelin Removal Beads II,Miltenyi Biotec,130-096-433 ,, Biological samples,Biological

(8) Glucocorticoids induce a phagocytic C1Q+ macrophage phenotype primed for IFNγ-dependent CXCL9 secretionSci RepMay 18, 2026Alexandra S. Triebig, Tanja Maier, Paul Schwarzlmueller, Sena Atici, Martin E. Kirmaier, Vasileios Chortis, Kleiton S. Borges, David T. Breault, Claudio Ribeiro, Adrian Gottschlich, et al.Following viability staining, cells were washed and incubated with Fc receptor blocking reagent (Biolegend, 422302) to prevent nonspecific antibody binding. After blocking, cells were stained with fluorochrome-conjugated

(9) Multiple sclerosis-associated HLA demarcates EBV-specific CD8+ T cells with an exhausted and brain residency phenotypeiScienceMay 15, 2026Sanne Reijm, Ana M. Marques, Jasper Rip, Cato E.A. Corsten, Annet F. Wierenga-Wolf, Harm de Wit, Marie-José Melief, Yifan van Hasselt, Jamie van Langelaar, Rinze Neuteboom, et al.AB_3097294 IFNγ-RB613 (Clone B27),BD Biosciences,Cat#571095; RRID: AB_3686229 Human TruStain FcX,BioLegend,Cat#422302; RRID: AB_2818986 ,, Bacterial and virus strains,Bacterial and virus strains,Bacterial and virus strains ,, HLA-A∗02:01

(10) Protocol for a multi-modal performance-profiling workflow of CAR T cell productsSTAR ProtocMay 15, 2026Sarah Schulenberg, Michelle Loeser, Michael Schmueck-Henneresse,BD Biosciences,Cat#563794 BD Horizon™ Brilliant Stain Buffer Plus,BD Biosciences,Cat#566385 Human TruStain FcX,Biolegend,Cat#422302 Zombie NIR Fixable Viability Kit,Biolegend,Cat#423106 eBioscience™ Foxp3/Transcription Factor Staining

(11) Feeder-free generation of functional dendritic cells from human pluripotent stem cellsJ Immunother CancerMay 13, 2026Wei-Hung Jung, Andrew Khalil, Yoav Binenbaum, Joshua M Price, Kyle H Vining, Tenzin Lungjangwa, Miguel C Sobral, Rudolf Jaenisch, David J Mooneytimes with FACS buffer (Invitrogen, 00–4222-26). For Fc receptor blocking, human Fc block (BioLegend, 422302) and Brilliant Stain Buffer (BD, 563794) were added to the cells and incubated for 10 min at 4°C. Corresponding

(12) Generation of Functional Patient-Specific Thymus Organoids From Human Pluripotent Stem Cells (hPSCs) Using Air–Liquid Interface CultureBio ProtocMay 5, 2026Stephan A. Ramos, Holger A. RussHuman serum (Gemini, catalog number: 100-512) 28. Human TruStain FcXTM (BioLegend, catalog number: 422302) 29. Hybridoma mix (Thermo Fisher Scientific, catalog number: 12045084) 30. Hydrocortisone (Sigma-Aldrich,

(13) Response of B cells specific for polyomavirus-derived oncoprotein is predictive of Merkel cell carcinoma tumor controlCancer Immunol ResMay 4, 2026Haroldo J. Rodriguez Chevez, Allison J. Remington, Matthew D. Gray, Rian Alam, Macy W. Gilmour, Carina Morningstar, Gabriel F. Alencar, Thomas Pulliam, Erin M. McClure, Neha Singh, et al.added, followed by a blocking buffer to bring samples to 0.5% BSA, 5% TruStain FcX buffer (BioLegend #422302), 100 nM dasatinib (Selleck Chem #S1021), and oligo-PE-GSTDL594 and APCDL755-GST control tetramers.

(14) Oncostatin M receptor deficiency as a novel candidate genetic cause of autosomal recessive hyper-IgE syndromeJ Hum ImmunMay 4, 2026Andersen S, Assing K, Jensen J, Rasmussen LD, Laursen CB, Dellgren CD, Hinke DM, Degn SE, Mogensen TH.(L10119; Invitrogen) in PBS at RT, washed with PBS, and FC blocked for 10 min using Human TruStain FcX (422302; BioLegend) in 1% BSA in PBS. Antibodies for staining of surface markers were directly added to the

(15) Ex vivo expansion of melanoma tumor infiltrating lymphocytes leads to a dominant exhausted T cell population with lack of memory markersCancer Immunol ResMay 4, 2026Gianfilippo Coppola, Samuel Kerr, Pei-Chun Cha, Alexey Bersenev, Kelly Olino, Harriet M. Kluger, Mario Sznol, Sarah A. Weiss, Marcus W. Bosenberg, Steven H. Kleinstein, et al.Buffer (1% BSA in PBS with 0.1% sodium azide). Cells were incubated with Human TruStain FcX (BioLegend #422302, RRID:AB_2818986) for 15 minutes on ice, then an antibody cocktail was added to the cells and incubated

(16) Nasal CD4+ tissue-resident memory T cells provide cross-protective immunity to influenzaJ Exp MedMay 4, 2026Nimitha R. Mathew, Romain Gailleton, Lydia Scharf, Karin Schön, Josue Enriquez, Hannes Axelsson, Anneli Strömberg, Nils Lycke, Mats Bemark, Ka-Wei Tang, et al.and flow cytometry of human PBMC and nasopharyngeal cells PBMC and NT cells were incubated with Human TruStain FcX (422302; BioLegend) for 10 min at 4°C. The cells were then incubated with anti-human CD69 BV421 antibody (clone:

(17) Establishment of Patient-Derived Organoids for Pediatric Cancer ResearchCancers (Basel)May 2, 2026Muhammad Younis, Tarlan Arjmandi, Mohammad Haque, Katherine McClain, Thussenthan Walter-Angelo, Franklin Back, Divya Gandra, Abigail Moore, Chandrika Behura, Vladimir S. Spiegelman, et al.Kit (Biowell; Westland, MI, USA; Cat No.: BM01), blocking reagent (BioLegend; San Diego, CA, USA; #422302) human CD45 (Invitrogen; Carlsbad, CA, USA; Cat # 58-0459-42), CD3 (Invitrogen Cat # 16-0037-81), B220

(18) Pancancer Fine‐Mapping of Mutational Intolerance Identifies CHEK1 as an Immunosuppressive Driver in Lung AdenocarcinomaAdvanced ScienceMay 1, 2026Tao Wang, Hongyu Zhao, Xiaojie Sun, Yuqi Ding, Zhipeng Zhu, Xiaotong Yu, Rongyi Zhu, Dan Wang, Kailong Li, Yang Liu, et al.prepared in ice‐cold PBS and incubated with Fc receptor blocking solution (Human TruStain FcX, BioLegend, #422302;) for 10 min at room temperature to prevent nonspecific antibody binding, and stained with CD80 PE (305207)

(19) Targeting WNK1 Releases Differentiation Block in Acute Myeloid LeukemiabioRxivApril 30, 2026Jordan D. Cress, Emily M. Katoni, Parameswaran Ramakrishnancontrol for 72 hours. The cells were pre-incubated with TruStain Human FcX Blocking Buffer (BioLegend, 422302) prior to staining with anti-CD11b (BioLegend, 101212) and anti-CD14 antibodies (BioLegend, 367104).

(20) Tissue-specific adaptation of human T cells is preserved during tissue inflammationbioRxivApril 30, 2026Nicole B. Potchen, Hugh R. MacMillan, Eva Domenjo-Vila, Andrew J. Konecny, Alexis K. Taber, Caitlin S. DeJong, Geervani Daggupati, Swati Shree, Stephen A. McCartney, Shelton W. Wright, et al.performed in the dark at room temperature in a 96-bottom plate. Cells were incubated with FcX block (422302, Biolegend) and Zombie NIR dye (422302, Biolegend) in PBS (14190250, Gibco) for 15 minutes. Antibody

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(1)

10/6/2023Great Reagent From Biolegend for Flow Blocking Danielle SchmidtWe use this block during flow cytometry before adding our panel antibodies. It does a great job blocking common proteins that we are not trying to measure
(1)

8/11/2023Great Fc Block From BioLegend Lana JagannathanI use this Fc block for a variety of staining purposes, mainly to stain monocyte-derived dendritic cells for flow cytometry. It has never let me down!
(1)

9/23/2022Reliable Fc Block for Flow Cytometry of Human Cells Vincent GiacaloneThis reagent is used to block Fc receptors on cells for flow cytometry staining to prevent non-specific binding of antibodies.
(1)

7/30/2020User Friendly Fc Receptor Blocker Rebecca GuyerGood Fc blocker to add to your antibody cocktails when staining for flow cytometry

Human TruStain FcX™ from BioLegend

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Human TruStain FcX™ from BioLegend

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