Human TruStain FcX™ from BioLegend

Product Specs
ItemHuman TruStain FcX™
CompanyBioLegend
Price $229.00Supplier Page View Company Product Page
Catalog Number422302
Quantity200 tests
ApplicationsFC - Quality tested, ICFC, ICC - Reported in the literature, not verified in house

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Adenine base editor for knockout of proteins: a practical guide from design to analysis with updated MultiEditRbatchMolecular Therapy Nucleic AcidsJune 16, 2026Ella J. Eaton, Bryce J. Wick, Jeremy S. Chacón, Amy J. Wang, Mitchell Kluesner, Jackson T. Barnes, Bibekananda Kar, Minjing Wang, Matthew J. Johnson, Joseph G. Skeate, et al.were stained using the BD Cytofix/Cytoperm kit (BD Biosciences), human TruStain FcX (BioLegend, Cat #422302), and antibodies listed in Table S3. Flow cytometry was performed on the CytoFlex S (Beckman Coulter)

(2) Protocol for culture, drug treatment, and dissociation of patient-derived 3D ovarian cancer microspheroids for flow cytometrySTAR ProtocolsJune 1, 2026Mariam Haffa, Karolina Juhani, Greta Gudoityte, Rita Hutyra-Gram Ötvös, Emelie Wallin, Olli-Pekka Kallioniemi, Josefin Fernebro, Ulrika Joneborg, Brinton Seashore-LudlowRESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Human TruStain FcX™ (FC Receptor Blocking Solution),BioLegend,Cat#422302; RRID: AB_2818986 "anti-human CD326-APC (EpCAM), clone: 9C4 (1:50 dilution)",BD Bioscience,CAT#566842;

(3) Oncostatin M receptor deficiency as a novel candidate genetic cause of autosomal recessive hyper-IgE syndromeJ Hum ImmunMay 4, 2026Sisse Andersen, Kristian Assing, Julie Jensen, Line Dahlerup Rasmussen, Christian B. Laursen, Christoffer D. Dellgren, Daniëla Maria Hinke, Søren E. Degn, Trine H. Mogensen(L10119; Invitrogen) in PBS at RT, washed with PBS, and FC blocked for 10 min using Human TruStain FcX (422302; BioLegend) in 1% BSA in PBS. Antibodies for staining of surface markers were directly added to the

(4) Nasal CD4+ tissue-resident memory T cells provide cross-protective immunity to influenzaJ Exp MedMay 4, 2026Nimitha R. Mathew, Romain Gailleton, Lydia Scharf, Karin Schön, Josue Enriquez, Hannes Axelsson, Anneli Strömberg, Nils Lycke, Mats Bemark, Ka-Wei Tang, et al.and flow cytometry of human PBMC and nasopharyngeal cells PBMC and NT cells were incubated with Human TruStain FcX (422302; BioLegend) for 10 min at 4°C. The cells were then incubated with anti-human CD69 BV421 antibody (clone:

(5) Creatine uptake promotes dendritic cell activation and enhances antitumor immunityiScienceApril 17, 2026Elliot Kang, James Elsten-Brown, Yu-Chen Wang, Ashley Lam, Elise Sanchez, Renee Wen, Tiffany Wang, Jennifer Chiang, Quentin Scarborough, Yan-Ruide Li, et al.CD25 (clone M-A251) were purchased from BioLegend. Human Fc Receptor Blocking Solution (catalog no. 422302) was purchased from BioLegend. Fixable Viability Dye eFluor 506 (catalog no. 65–0866) was purchased

(6) A multimodal atlas for immunotherapeutic targeting of AML surface heterogeneityiScienceApril 17, 2026Matthew Ung, Julia Etchin, Amanda Halfond, Julia DiFazio, Yonina Keschner, Alyssa Pyclik, Anne Campbell, Ruijia Wang, Mariana Silva, Brikena Gjeci, et al.1640,Gibco,Cat# 11875093 Fetal Bovine Serum,Corning,Cat# 35-011-CV Human TruStain FcX™,"BioLegend, LLC",Cat# 422302 True-Stain Monocyte Blocker™,"BioLegend, LLC",Cat# 426103 P3 100 Cycles SBS Reagent Kit,Illumina Inc,Cat#

(7) Translational strategy to support the first-in-human study of a TCR-like T cell bispecific with an in vitro-based safety approachFront ImmunolApril 17, 2026Angelique Augustin, Blandine Avignon, Christophe Boetsch, Ekaterina Breous-Nystrom, Olaf Broders, Lauriane Cabon, Karen Dernick, Evelyne Durr, Miro Julian Eigenmann, Susanne Fischer, et al.were blocked with True Stain Monocyte Blocker (Cat: 426103, BioLegend) and Human TruStain FcX (Cat: 422302, BioLegend) followed by staining with the following antibody panel: Lineage Markers: CD3-BV421 (Cat:

(8) Ex vivo cytokine responses and metabolic reprogramming are associated with γδ T cell differentiation in Plasmodium falciparum placental malariaFront ImmunolApril 16, 2026Chris Marco Nana Mbianda, Bodin Darcisse Kwanou Tchakounte, Bernard Marie Bitye Zambo, Joséphine Ntankheuh Tchinkou, Derrick Atchombat Nyasse, Balotin Fogang, Rafael José Argüello, Lawrence Ayong, Rosette Megnekoumin with a fixable viability dye. Cells were again washed, incubated with Fc Block (BioLegend, cat#422302, USA), and stained with conjugated antibodies for 30 min at 4 °C in FACS Buffer. Cells were then fixed

(9) Ionizing radiation expands a p90RSK-activated patrolling monocyte subset: modulation by colchicineFront Cardiovasc MedApril 15, 2026Masaki Imanishi, Venkata S. K. Samanthapudi, Nhat-Tu Le, Luis Antonio Rivera, Jung Hyun Kim, Jonghae Lee, Gilbert F. Mejia, Oanh Hoang, Anita Deswal, Keri L. Schadler, et al.in MaxPar Cell Staining Buffer (Fluidigm #201068) and were blocked by Human TruStain FcX (BioLegend #422302), followed by cell surface staining for cell type identification. After the wash with MaxPar PBS, the

(10) CD38 expression by neonatal human naïve CD4+ T cells shapes their distinct metabolic and tolerogenic propertiesJournal of Clinical InvestigationApril 15, 2026Laura R. Dwyer, Andrea M. DeRogatis, Sean Clancy, Victoire Gouirand, Charles Chien, Elizabeth E. Rogers, Scott P. Oltman, Laura L. Jelliffe-Pawlowski, Theo van den Broek, Femke van Wijk, et al.Antibody cocktails were prepared in cell staining buffer with 20 mg/mL Human TruStain-FcX (BioLegend, 422302) (surface) or in Foxp3 buffer (intracellular) (eBioscience, 00-5523-00). Pooled samples were surface-stained

(11) Ultra-large targeted DNA integrations in primary human cellsbioRxivApril 9, 2026Courtney Kernick, Lauren Chow, Mikail Alejandro, Ke Li, Maxwell G. Foisey, Xiaoyu Yang, Claire Hilburger, Johnathan Lu, Lujing Wu, Alison McClellan, et al.anti-Streptavidin. To stain for PDAC-synNotch, cells were incubated with 50x diluted Fc block (BioLegend, 422302) in FACS buffer at room temperature for 10 minutes prior to surface staining. Knock-in was assessed

(12) An at-home blood collection device for remote immune monitoring by high-parameter flow cytometryJCI InsightApril 8, 2026Andrew J. Konecny, Fang Yun Lim, Eva Domenjo-Vila, Erika Lovas, Rachel L. Blazevic, Louise E. Kimball, Michael Boeckh, Alpana Waghmare, Martin Prlictemperature in round-bottom 96-well plates. After thawing, cells were incubated with Trustain FcX (BioLegend, 422302), a blocking reagent for Fc receptors, and Live/Dead Fixable Blue Dead Cell stain (Invitrogen, L23105)

(13) High-Dimensional Protein Analysis Uncovers Distinct Immunological and Stromal Features in Primary and Metastatic Pancreatic Ductal AdenocarcinomaCancer ResearchApril 2, 2026Emily Greene, Natalie K. Horvat, Deon Bryant. Doxie, Vaunita Cohen. Parihar, Jayden Kim, Cameron J. Herting, Erin E. Grundy, Ayana T. Ruffin, Alyssa M. Krasinskas, Shishir K. Maithel, et al.steamer. Slides were washed twice with PBS for 5 minutes and sequentially blocked with Human TruStain FcX (422302, BioLegend) for 60 minutes at room temperature at 1:200 in PBS and then using 5% donkey serum (GTX732205,

(14) CD8 TRM-like T cells expressing perforin and IFN-γ define a pediatric activated T-cell acute liver failure endotypeHepatol CommunApril 1, 2026Tamir Diamond, Catherine A. Chapin, Caroline J. Diorio, Sarah McCuaig, Michelle Lau, Niansheng Chu, Nora M. Gibson, Portia A. Kreiger, Simon Horslen, Kathleen M. Loomes, et al.live cell suspensions were blocked using 5 μL of human TruStain FcX Fc Blocking reagent (BioLegend, 422302). Subsequently, they were incubated with 1 μL (0.5 μg) of the respective TotalSeq-B anti-human Hashtag

(15) IDH1 Mutant Glioma Favors Group 3 Innate Lymphoid Cells and Is Resistant to Immune Checkpoint ExpressionInflamm ResApril 1, 2026Serife Erdem, Yesim Haliloglu, Inayet Nur Uslu, Mohammad Houran, Halil Ulutabanca, Alperen Vural, Mehmet Berat Erturhan, Halit Canatan, Ahmet Ekenat 4 °C. Fc receptors were inhibited by incubating cells with Human TruStain FcX™ (BioLegend, Cat# 422302) for a duration of 15 min. Cells were incubated in the dark at 4 °C for 30 min with the following antibodies:

(16) NAMPT inhibition induces ferroptosis via mitochondrial metabolic reprogramming to enhance tumour immunogenicity in glioblastomaFrontiers in ImmunologyMarch 31, 2026Dongxu Zhao, Lin Duan, Hang Ren, Hang Zhang, Menghui Zhang, Zhuohang Liu, Hailan Wang, Wenxuan Lu, Wenhan Shi, Qingwang Hou, et al.from co-cultured THP-1 and RAW264.7 cells. Cells were initially incubated with Fc Block (BioLegend, 422302) for 10 min at 4 °C to prevent nonspecific antibody binding. Subsequently, cells were stained with fluorophore-conjugated

(17) Profiling Peripheral Blood with an Optimized, Multiplexed, Single-cell Multiome Approach Supports an Insulin-driven Asthma SubtypebioRxivMarch 31, 2026Jiacheng Ding, HyunJin Kang, Amber L. Spangenberg, Yusha Liu, Fernando D. Martinez, Tara F. Carr, Darren A. Cusanovichchange the buffer to 100 ul Staining Buffer (2% BSA in PBS), followed by 10% TruStain FcX (Biolegend #422302) staining at 10 minutes at room temperature. After the blocking reaction, 300 ul of Staining Buffer,

(18) CD8 TRM-like T cells expressing perforin and IFN-γ define a pediatric activated T-cell acute liver failure endotypeHepatol CommunMarch 31, 2026Tamir Diamond, Catherine A. Chapin, Caroline J. Diorio, Sarah McCuaig, Michelle Lau, Niansheng Chu, Nora M. Gibson, Portia A. Kreiger, Simon Horslen, Kathleen M. Loomes, et al.live cell suspensions were blocked using 5 μL of human TruStain FcX Fc Blocking reagent (BioLegend, 422302). Subsequently, they were incubated with 1 μL (0.5 μg) of the respective TotalSeq-B anti-human Hashtag

(19) Baseline Th17/Tc17 and LAG-3 levels serve as candidate exploratory markers for early ixekizumab response in psoriasisFrontiers in ImmunologyMarch 27, 2026Nan Yang, Zeyu Chen, Yuxiong Jiang, Jiangluyi Cai, Lian Cui, Yuanyuan Wang, Suyang Lin, Ling Wu, Qian Du, Jun Gu, et al.hours before staining. Single-cell suspensions were pre-incubated with Fc Receptor Blocking Solution (#422302, BioLegend) for 10 minutes at room temperature. To discriminate dead cells, the cells were stained with

(20) Krüppel-like factors 2 and 3 regulate T cell exhaustion by directing T cell residency and migrationImmunityMarch 27, 2026Jian Shen, Ryan J. Brown, Yuqi Zhang, Kexin Gai, Ashley K. Brown, Ashley M. Bauer, Ashley Zhu, Siying Lin, Hongshen Niu, Arjun Kharel, et al./ eBioscience,Cat# 11-5698-82; RRID: AB_11220070 TruStain FcX (Anti-mouse CD16/32),BioLegend,Cat# 422302; RRID: AB_2818986 "Anti-mouse CD3e (145-2C11), Functional Grade",Thermo Fisher / eBioscience,Cat# 16-0031-86;

Write a Review

(1)

10/6/2023Great Reagent From Biolegend for Flow Blocking Danielle SchmidtWe use this block during flow cytometry before adding our panel antibodies. It does a great job blocking common proteins that we are not trying to measure
(1)

8/11/2023Great Fc Block From BioLegend Lana JagannathanI use this Fc block for a variety of staining purposes, mainly to stain monocyte-derived dendritic cells for flow cytometry. It has never let me down!
(1)

9/23/2022Reliable Fc Block for Flow Cytometry of Human Cells Vincent GiacaloneThis reagent is used to block Fc receptors on cells for flow cytometry staining to prevent non-specific binding of antibodies.
(1)

7/30/2020User Friendly Fc Receptor Blocker Rebecca GuyerGood Fc blocker to add to your antibody cocktails when staining for flow cytometry

Human TruStain FcX™ from BioLegend

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Human TruStain FcX™ from BioLegend

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