Human TruStain FcX™ from BioLegend

Product Specs
ItemHuman TruStain FcX™
CompanyBioLegend
Price $229.00Supplier Page View Company Product Page
Catalog Number422302
Quantity200 tests
ApplicationsFC - Quality tested, ICFC, ICC - Reported in the literature, not verified in house

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Tumoral and Systemic Immune Correlates of Response to Concurrent Pembrolizumab and Chemoradiotherapy in Patients with Resected High-Risk Head and Neck Squamous Cell CarcinomaClin Cancer ResJune 25, 2026Lazar Vujanovic, Pedro Torres-Saavedra, Fei Tu, Ravindra Uppaluri, Min Yao, Josephine Chen, Richard Jordan, Jessica L Geiger, Srinivas Jujjavarapu, Arnab Chakravati, et al.(FACS) buffer (1× PBS containing 0.2% BSA and 0.02% EDTA; Thermo Fisher). Human TruStain FcX (Cat# 422302; RRID: AB_2818986; BioLegend; San Diego, CA) was used to block non-specific antibody binding for 15

(2) Targeting non-canonical antigens unlocks functional T-cell responses in renal cell carcinomaJ Immunother CancerJune 24, 2026Jiahao Wang, Yihao Zhu, Xinyu He, Shenyi Yin, Yang He, Pengju Yao, Juan Li, Xuwen Li, Peiting Shi, Renzhi Qian, et al.procedure described in the interferon (IFN)-γ ELISpot assay section. Human TruStain FcX (BioLegend, Cat#422302), 7-AAD (BioLegend, Cat#420403), anti-human CD3-PE (BioLegend, Cat#300907), anti-human CD8-APC (BioLegend,

(3) Distinct CD8+ T-cell Types Associated with COVID-19 Severity in Unvaccinated HLA-A2+ PatientsiScienceJune 19, 2026Kazuya Masuda, Sho Iketani, Lihong Liu, Jing Huang, Yujie Qiao, Jayesh Shah, Meredith L. McNairy, Christine Groso, Christopher L. Ricupero, Lucas F. Loffredo, et al.incubation, the cells were washed 3 times and incubated for 10 minutes with Fc receptor block (BioLegend, 422302). Afterward, each sample was stained with a total of 6 HLA-A2+/peptide fluorescent tetramer (Tet) and

(4) Adenine base editor for knockout of proteins: a practical guide from design to analysis with updated MultiEditRbatchMolecular Therapy Nucleic AcidsJune 16, 2026Ella J. Eaton, Bryce J. Wick, Jeremy S. Chacón, Amy J. Wang, Mitchell Kluesner, Jackson T. Barnes, Bibekananda Kar, Minjing Wang, Matthew J. Johnson, Joseph G. Skeate, et al.were stained using the BD Cytofix/Cytoperm kit (BD Biosciences), human TruStain FcX (BioLegend, Cat #422302), and antibodies listed in Table S3. Flow cytometry was performed on the CytoFlex S (Beckman Coulter)

(5) Type I interferon signaling in hematopoietic cells impairs neutrophil antibacterial function in the middle ear during viral co-infectionCell Rep MedJune 16, 2026Steven C. Shaw, Taylor L. Jamil, Gabriela Heslop, Jeremy T. Fleck, Wyatt Johnson, Brian P. Lorenz, Zoe Drigot, J. Kirk Harris, Sarah A. Gitomer, Sarah E. Clarkand resuspended in Fc block (BioLegend, catalog #101302, lot #B423718 for mouse samples or catalog #422302 lot#B391743 for human cells) and incubated at 4°C for 20 min. For surface staining, cells were incubated

(6) FTL008.16, a 5T4-Conditional 4-1BB bispecific antibody, potently enhances antitumor immunity via tumor-directed t-cell activationOncoimmunologyJune 10, 2026Shuang Liu, Ziqing Ren, Yongyan Tu, Pengyu Chen, Yinglu Zhang, Shengyan Zhao, Xiaofeng Chen, Han Deng, Shuyun Yang, Feng Qu, et al.incubation at 37 °C, cells were harvested, blocked with Fc receptor blocking solution (BioLegend, #422302), and stained with 7-AAD (BD, #559925) for viability, as well as anti-CD3-PE (BD, #555340) and anti-CD8-APC

(7) Dietary sulfur amino acids enhance anti-tumor immunity in colon cancer via an NKT cell-XCL1-cDC1 circuitImmunityJune 9, 2026Lior Lobel, Diogo Fonseca-Pereira, Geicho Nakatsu, Monia Michaud, Y. Grace Cao, Sena Bae, Cathleen Krabak, Yaara Oren, Slater L. Clay, Geniver El El Tekle, et al.6B11),BioLegend,Cat# 342904; RRID: AB_2078494 Human TruStain FcX™ (Fc Receptor Blocking Solution),BioLegend,Cat# 422302; RRID: AB_2818986 BD Pharmingen™ Purified Rat Anti-Mouse IL-2 (Clone JES6-1A12),BD Biosciences,Cat#

(8) Live-cell Pick-Seq (LiP-Seq): Interrogating ultra-rare mantle cell lymphoma persistent cells after CART19 therapyBlood AdvancesJune 9, 2026Ran Xu, Gongwei Wu, Siobhan Rice, Matthew Booker, Jenalyn Celine Weekes, Hayden Luke Bell, Yifei Gao, Ajit J. Nirmal, Huiyun Liu, Zoe Ciantra, et al.catalog nos. 17876 and 17851). Next, cells were incubated with 5 μL of Fc block (BioLegend, catalog no. 422302) in 100 μL fluorescence-activated cell sorter (FACS) buffer on ice for 10 minutes followed by addition

(9) Protocol for culture, drug treatment, and dissociation of patient-derived 3D ovarian cancer microspheroids for flow cytometrySTAR ProtocolsJune 1, 2026Mariam Haffa, Karolina Juhani, Greta Gudoityte, Rita Hutyra-Gram Ötvös, Emelie Wallin, Olli-Pekka Kallioniemi, Josefin Fernebro, Ulrika Joneborg, Brinton Seashore-LudlowRESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Human TruStain FcX™ (FC Receptor Blocking Solution),BioLegend,Cat#422302; RRID: AB_2818986 "anti-human CD326-APC (EpCAM), clone: 9C4 (1:50 dilution)",BD Bioscience,CAT#566842;

(10) CCL19⁺ fibroblasts define a proliferative niche in chronic lymphocytic leukemiabioRxivMay 30, 2026Antonio P. Ferreira, Shumei Wang, Larysa Poluben, Dominique Morais, Joshua Brandstadter, Eric Perkey, Steven Sotirakos, Mai Drew, Li Pan, Madeleine E. Lemieux, et al.#420301). Cells in suspension were then incubated for 10 min with Fc Blocking Reagent (Biolegend, cat. #422302) followed by DAPI (Biolegend, 1 μg/mL, cat. #422801) in FACS buffer (phosphate buffered saline [PBS]

(11) High-dimensional immune profiling of peripheral blood identifies immune correlates of anti-PD-1/PD-L1 resistance in oncogenic driver mutation-positive NSCLCFrontiers in ImmunologyMay 29, 2026Kyoo Hyun Kim, Mina Han, Geon Woo Park, Min Hee Hong, Gamin Kim, Brian Hyohyoung Lee, Wonrak Son, Jaehyung Kim, Seokhyeong Go, Jeongah Kim, et al.Following incubation, the samples were washed and blocked with an Fc receptor–blocking solution (Cat #422302; BioLegend) to prevent non-specific antibody binding. Each sample from the same patient was subsequently

(12) Spatial Regulation of CAR Signaling Enables Logic-Gated ActivitybioRxivMay 27, 2026Tushar D. Nichakawade, Brian J. Mog, Alexander H. Pearlman, Taha S. Ahmedna, Nikita Marcou, Ricardo Mora-Cartin, Evangeline Watson, Bum Seok Lee, Sarah R. DiNapoli, Nickolas Papadopoulos, et al.were first blocked using TruStain FcX PLUS (Biolegend, #156604) and/or Human TruStain FcX (Biolegend, #422302) in 50 μL of flow staining buffer for 10 minutes. Then the desired antibody cocktails were added for

(13) Dual Roles of hABCB1 in Drug Resistance and Immune Evasion: Implications for Lung Cancer TherapyACS OmegaMay 26, 2026Jin Young Min, Hye Min Kim, Geul Bang, Hae Won Jung, Yea Jin Kim, Sang-Yeop Lee, Dong Joon Kim, Sang Keun Ha, Truc Ngoc Kim Lam, Kwan Soo Hong, et al.incubation with an Fc receptor blocker (Human TruStain FcX; BioLegend, San Diego, CA, USA; cat. no. 422302) for 30 min at 4 °C, cells (1 × 105) were stained at 4 °C for 20 min in the dark using the following

(14) Protocol for assessing anti-Siglec-9 antibody-mediated tumor phagocytosis by macrophages using pH-sensitive dyes and flow cytometrySTAR ProtocMay 20, 2026Saitong Muneekaew, Thunchanok Nuchphongsai, Pasut Sasithong, Kritayaporn Saiprayong, Kamonphan Rungrueang, Methichit Wattanapanitch(1:500 dilution),Biolegend,Cat. No. 423102 Human TruStain FcX (TM) (1:20 dilution),BioLegend,Cat. No. 422302 True-Stain Monocyte Blocker (1:20 dilution),BioLegend,Cat. No. 426120 ,, Biological samples,Biological

(15) A Novel PTPN2 Isoform Differentially Regulates Immune ResponseEur J ImmunolMay 19, 2026Xiaoguang Li, Fedik Rahimov, Rui Wang, Lei Gao, Renze Ma, Tiankai Han, Yingtao Bi, Freidrich Cruz, Jing Wang, Shaun Grosskurth, et al.dead cells. Cells were first blocked with Human TruStain FcX (Fc Receptor Blocking Solution) (Catalog# 422302, BioLegend) using a 1:100 dilution and then stained for 30 min at 4°C with staining antibodies. moDCs

(16) Glucocorticoids induce a phagocytic C1Q+ macrophage phenotype primed for IFNγ-dependent CXCL9 secretionSci RepMay 18, 2026Alexandra S. Triebig, Tanja Maier, Paul Schwarzlmueller, Sena Atici, Martin E. Kirmaier, Vasileios Chortis, Kleiton S. Borges, David T. Breault, Claudio Ribeiro, Adrian Gottschlich, et al.Following viability staining, cells were washed and incubated with Fc receptor blocking reagent (Biolegend, 422302) to prevent nonspecific antibody binding. After blocking, cells were stained with fluorochrome-conjugated

(17) An improved preservation method for human dorsal root ganglion neurons enables wider access to human molecular pain neuroscienceCell Reports MethodsMay 18, 2026Joseph B. Lesnak, Mandee K. Schaub, Kimberly Gomez, Aida Calderon-Rivera, Santiago Loya-Lopez, Robert Stewart, Sooyeon Jo, Akie Fujita, Tomás Osorno, Hemanth Mydugolam, et al.RRID: AB_314065 Zombie UV viability dye,BioLegend,423107 TruStain FcX (human Fc block),BioLegend,422302; RRID:AB_28118986 Myelin Removal Beads II,Miltenyi Biotec,130-096-433 ,, Biological samples,Biological

(18) Protocol for a multi-modal performance-profiling workflow of CAR T cell productsSTAR ProtocMay 15, 2026Sarah Schulenberg, Michelle Loeser, Michael Schmueck-Henneresse,BD Biosciences,Cat#563794 BD Horizon™ Brilliant Stain Buffer Plus,BD Biosciences,Cat#566385 Human TruStain FcX,Biolegend,Cat#422302 Zombie NIR Fixable Viability Kit,Biolegend,Cat#423106 eBioscience™ Foxp3/Transcription Factor Staining

(19) Multiple sclerosis-associated HLA demarcates EBV-specific CD8+ T cells with an exhausted and brain residency phenotypeiScienceMay 15, 2026Sanne Reijm, Ana M. Marques, Jasper Rip, Cato E.A. Corsten, Annet F. Wierenga-Wolf, Harm de Wit, Marie-José Melief, Yifan van Hasselt, Jamie van Langelaar, Rinze Neuteboom, et al.AB_3097294 IFNγ-RB613 (Clone B27),BD Biosciences,Cat#571095; RRID: AB_3686229 Human TruStain FcX,BioLegend,Cat#422302; RRID: AB_2818986 ,, Bacterial and virus strains,Bacterial and virus strains,Bacterial and virus strains ,, HLA-A∗02:01

(20) Feeder-free generation of functional dendritic cells from human pluripotent stem cellsJ Immunother CancerMay 13, 2026Wei-Hung Jung, Andrew Khalil, Yoav Binenbaum, Joshua M Price, Kyle H Vining, Tenzin Lungjangwa, Miguel C Sobral, Rudolf Jaenisch, David J Mooneytimes with FACS buffer (Invitrogen, 00–4222-26). For Fc receptor blocking, human Fc block (BioLegend, 422302) and Brilliant Stain Buffer (BD, 563794) were added to the cells and incubated for 10 min at 4°C. Corresponding

Write a Review

(1)

10/6/2023Great Reagent From Biolegend for Flow Blocking Danielle SchmidtWe use this block during flow cytometry before adding our panel antibodies. It does a great job blocking common proteins that we are not trying to measure
(1)

8/11/2023Great Fc Block From BioLegend Lana JagannathanI use this Fc block for a variety of staining purposes, mainly to stain monocyte-derived dendritic cells for flow cytometry. It has never let me down!
(1)

9/23/2022Reliable Fc Block for Flow Cytometry of Human Cells Vincent GiacaloneThis reagent is used to block Fc receptors on cells for flow cytometry staining to prevent non-specific binding of antibodies.
(1)

7/30/2020User Friendly Fc Receptor Blocker Rebecca GuyerGood Fc blocker to add to your antibody cocktails when staining for flow cytometry

Human TruStain FcX™ from BioLegend

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Human TruStain FcX™ from BioLegend

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