Great Reagent From Biolegend for Flow Blocking

October 06, 2023

BlueskyReddit
Cancer Center
The George Washington University
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Human trustain Fcx

Catalog Number:

422302

Clone Number:

N/A

We use this block during flow cytometry before adding our panel antibodies. It does a great job blocking common proteins that we are not trying to measure

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

We use T cells

Primary Incubation

5uL Fc block per well in 95uL FACS buffer, 10 minutes at 4 degrees

Blocking Agent

Human Trustain Fc

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

Successfully blocked proteins that we were not trying to measure before adding our panel antibodies.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Worked great.

The Bad

None thus far.

The Bottom Line

Blocks well so antibody signal can shine strong and accurate.

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