DAPI from Cell Signaling Technology

Product Specs
ItemDAPI
CompanyCell Signaling Technology
Price $74.00 Inquire
Catalog Number4083S
Quantity1 mg
Research AreaRelated Products
ApplicationsIF-IC, FC-FP

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(61) Ageing‐Dependent Thyroid Hormone Receptor α Reduction Activates IP3R1‐Meditated Ca2+ Transfer in MAM and Exacerbates Skeletal Muscle Atrophy in MiceCell ProliferationAugust 24, 2025Runqing Shi, Yusheng Zhang, Gong Chen, Jiru Zhang, Jing Liu, Hao Zhu, Minne Sun, Yu Duanwas performed the following day. The nuclei were stained with 4,6‐diamidino‐2‐phenylindole (DAPI, #4083, CST). 2.12 Mitochondrial Fluorescent Staining To detect MMP, ROS, Ca2+, and mitochondria, respectively,

(62) NEDD4L-Mediated Ubiquitination of GPX4 Exacerbates Doxorubicin-Induced CardiotoxicityInternational Journal of Molecular SciencesAugust 23, 2025Jiaxing Ke, Lingjia Li, Shuling Chen, Chenxin Liao, Feng Peng, Dajun Chai, Jinxiu LinImmunoway (Plano, TX, USA). Antibodies against ubiquitin (Ub, 43124), Flag (70586), Myc (2276), and DAPI (4083S) were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies against K48-linkage-specific

(63) Targeting G1–S-checkpoint-compromised cancers with cyclin A/B RxL inhibitorsNatureAugust 20, 2025Shilpa Singh, Catherine E. Gleason, Min Fang, Yasmin N. Laimon, Vishal Khivansara, Shanhai Xie, Yavuz T. Durmaz, Aniruddha Sarkar, Kenneth Ngo, Varunika Savla, et al.in PBS containing 0.5% BSA, centrifuged at 400g and then counterstained with DAPI) (Cell Signaling, 4083) for 15 min at room temperature. FACS analysis was then performed to determine the percentage of positive

(64) miR-9-5p alleviates the development of abdominal aortic aneurysm by regulating the differentiation of CD4+IL-10+T cells via targeting the crosstalk between Nrf2 and NF-κB signaling pathwaysTurkish Journal of BiologyAugust 8, 2025HONGFU LIU, JINYI ZHANG, LUBIN LI, BENXIANG YU, CHUNLEI ZHANG, WENQIANG NIU, YAWEN CHENG, HENGYANG DONG, YUKUN ZHANG, XINLIN LUO, et al.T cells, and consequently clinically reduce AAA lesions. 2. Materials and methods 2.1. Reagents DAPI(#4083) and antibodies against Histon H3 (# 4499 ), Nrf2 (#12721), NF-κB (#8242), β-actin (# 4970 ), HO-1 (#43966),

(65) Increased in vivo transduction of AAV-9 cargo in Alport podocytesWellcome Open ResearchJuly 30, 2025Emily Williams, Maryline Fresquet, Gema Bolas, Shota Kaseda, Kevin A. Goncalves, Andrew Steinsapir, Antony Adamson, David R. Sherwood, Rachel LennonAbcam Cat# ab150130; RRID:AB_2927775) for 1 hour at room temperature. DAPI (1:2000, Cell Signaling 4083) was used for nuclear staining. Images were collected on a Zeiss Axioimager.D2 upright microscope using

(66) Rapid reconstruction of infectious bronchitis virus expressing fluorescent protein from its nsp2 gene based on transformation-associated recombination platformJournal of VirologyJuly 22, 2025Yingfei Li, Linqing Duan, Lihua Tang, Min Huang, Ye Zhao, Guozhong Zhang, Jing Zhaowashed three times with PBS. Following nuclear counterstaining with 4′,6-diamidino-2-phenylindole (CST, #4083) for 5 min at room temperature, fluorescence imaging was performed using a Nikon camera. Growth kinetics

(67) Photoreceptor loss does not recruit neutrophils despite strong microglial activationElifeJuly 22, 2025Derek Power, Justin Elstrott, Jesse Schallekantibody (1:200, #127610, RRID:AB_1134159, BioLegend, San Diego, CA) and DAPI (1:500 of 10 mg/ml stock, #4083, Cell Signaling Technology, Danvers, MA) were diluted in 1× perm/wash buffer and retinas were incubated

(68) Secreted EMC10 inhibits muscle GLUT4 activity and glucose uptake in miceJournal of Biological ChemistryJuly 1, 2025Shuoshuo Jin, Wei Wu, Shan Liu, Yahao Wang, Qi Huang, Kun He, Yunzhi Ni, Kuangyang Chen, Jinya Huang, Lijie Liu, et al.at room temperature followed by an additional 10-min incubation with 4′,6-diamidino-2-phenylindole (4083; Cell Signaling Technology). The fluorescent images were captured with a fluorescence microscope (Leica). PET–CT

(69) New molecular components of high and low affinity iron import systems in DrosophilaNat CommunJuly 1, 2025Sattar Soltani, Minyi Yan, Qingxuan Yu, Areeg Abd Elhafiz, Erika Pfriem, Samuel M. Webb, Thomas Kroll, Jahir Marceliano Bahena Lopez, Fanis Missirlis, Kirst King-Jones1:2000 dilution). Samples were rinsed 3× in 1X PBST and mounted in VECTASHIELD solution (Cell signaling #4083). All images were taken with a Nikon C2si Confocal Microscope. RNA-Seq data analysis Sequencing services

(70) SIK2 inhibitor SIC-19 enhances the sensitivity of PARP inhibitors in triple-negative breast cancers and pancreatic cancersOncology ResearchJune 26, 2025QIAN LI, SHUNPENG ZHU, MINGXIAN ZHU, FANG WANG, JINHUA ZHOUFollowing that, the nuclei were stained for ten minutes using 4′,6-diamino-2-phenylindole (DAPI) (CST, 4083S, Danvers, MA, USA). A Zeiss microscope (LSM980, Carl Zeiss, Oberkochen, Germany) with a 63× objective

(71) Therapeutic potential of targeting the NEDD4L-eEF1A1 axis in cancer therapyActa Biochimica et Biophysica SinicaJune 25, 2025Yan Qin, Xinyue Wang, Minghui Zhao, Yuehui Liu, Huiyi Hou, Tingting Wang, Yuhe Pei, Jingxin Zhang, Zhou Shen, Feixiang Wu, et al.and mouse IgG were used. The nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (4083S; Cell Signaling Technology), and fluorescence images were captured via an Olympus FV3000 confocal microscope

(72) Viscoelastic N‑cadherin-like interactions maintain neural progenitor cell stemness within 3D matricesNat CommunJune 5, 2025Michelle S. Huang, Bauer L. LeSavage, Sadegh Ghorbani, Aidan E. Gilchrist, Julien G. Roth, Carla Huerta-López, Esther A. Mozipo, Renato S. Navarro, Sarah C. HeilshornFluor 647 (1:500, Invitrogen, A-21449). 4′,6-diamidino-2-phenylindole (DAPI; 1 μg/mL, Cell Signaling, 4083s) was included in the secondary antibody solution to stain nuclei. Samples were incubated overnight at

(73) Cleavage of CAD by caspase-3 determines the cancer cell fate during chemotherapyNat CommunMay 30, 2025Jingsong Ma, Jiabao Zhao, Chensong Zhang, Jinshui Tan, Ao Cheng, Zhuo Niu, Zeyang Lin, Guangchao Pan, Chao Chen, Yang Ding, et al.(BSA) in PBS at room temperature for 1 h before incubation with the primary antibodies against DAPI (#4083, CST) and Phospho-Histone H2A.X (Ser139) (#9718, CST) at 4 °C overnight. After dewaxing and receiving

(74) PML Regulated HIF1AN Ubiquitination and Activated PI3K/AKT Pathway to Promote Bone Marrow Mesenchymal Stem Cells Osteogenic DifferentiationInternational Journal of Stem CellsMay 30, 2025Xian-Pei Zhou, Qi-Wei Li, Zi-Zhen Shu, Yang Liuantibody (1：500, ab150077; Abcam) at 37℃ for 30 minutes. Finally, nuclei were counterstained with DAPI (4083; Cell Signaling Technology) for 10 minutes and images were obtained by a confocal microscope (Fluoview

(75) A retinoic acid:YAP1 signaling axis controls atrial lineage commitmentCell reportsMay 27, 2025Abraham E, Kostina A, Volmert B, Roule T, Huang L, Yu J, Williams AE, Megill E, Douglas A, Pericak OM, et al.6434;RRID:AB_11220428 CHCHD2,Abcam,Cat # ab220688;RRID:AB_369572 DAPI,Cell Signaling Technologies,Cat # 4083S;RRID:AB_3695726 GATA3,R & D Systems,Cat # AF2605-SP;RRID:AB_2108571 GAPDH,Protein Tech,Cat # 60004-1-Ig;RRID:AB_2107436 HAND1,Invitrogen,Cat

(76) LDHA-mediated YAP lactylation promotes the tumor progression of hepatocellular carcinoma by inducing YAP dephosphorylation and activationBiol DirectMay 26, 2025Xiaoyong Wei, Long Zou, Yanqing Huang, Chuan Qiu, Guang Cheng, Ye Chen, Jun RaoUSA, 7076 V) were added and incubated for 1 h. The cells were counterstained with DAPI (CST, MA, USA, 4083 S) and observed under a microscope. In vivo tumor xenograft study 24 five-week-old female nude mice

(77) BACE1 Inhibition Protects Against Type 2 Diabetes Mellitus by Restoring Insulin Receptor in MiceInternational Journal of Molecular SciencesMay 26, 2025Tingting Lin, Ting Liang, Yong Shen, Feng GaoAnti-rabbit Alexa Fluor® 555 secondary antibody (A-11004, Invitrogen, Carlsbad, CA, USA) containing DAPI (4083, Cell signaling technology, Danvers, MA, USA) for 1 h at room temperature and washed 5 times in PBS.

(78) Inhibition of xanthine oxidoreductase with febuxostat, but not allopurinol, prevents inflammasome assembly and IL-1β releaseLife Sci AllianceMay 21, 2025Lauar de Brito Monteiro, Anne-Sophie Archambault, Lucas F Starchuk, Armando Alcazar, Ju Hee Oh, Joshua A Dubland, Bojana Rakić, Annette E Patterson, C Bruce Verchere, Ramon I Klein Geltink647 Conjugate, #4414; Cell Signaling). Samples were rinsed with TBS+0.1% Tween, and DAPI (1 μg/ml; #4083; Cell Signaling) was added for 5 min. Samples were rinsed and resuspended with PBS for image acquisition

(79) Antisense oligonucleotides targeting IRF4 alleviate psoriasisActa Pharmaceutica Sinica BMay 14, 2025Yanxia Yu, Yirui Wang, Weiwei Chen, Chang Zhang, Zhuo li, Jing Yu, Minhao Wang, Can Song, Sihao Yan, Jiayi Lu, et al.dilution; BioLegend) at 4 °C away from light. The nuclei were stained with 4′,6-diamidino-2-phenylindole (#4083S; Cell Signaling Technology). The slides were sealed and images were captured at 40 × using a confocal

(80) Endothelial TIE2 Mutation Induced Contraction Deficiency of Vascular Smooth Muscle Cells via Phenotypic Transition Regulation in Venous MalformationsInt J Med SciMay 7, 2025Zhong Du, Fan Yu, Yuan He You, Zhi Yang Zhao, Zhuo Wei Tian, Meng Xiao, Yan An WangAffiniPure Donkey Anti-Mouse, Jackson, USA), overnight at 4 ℃, and nucleus was stained with DAPI (1:200, 4083, CST, USA). Images were finally scanned by an inverted fluorescence microscope (Nikon, Tokyo, Japan,

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