DAPI from Cell Signaling Technology

Product Specs
ItemDAPI
CompanyCell Signaling Technology
Price $74.00 Inquire
Catalog Number4083S
Quantity1 mg
Research AreaRelated Products
ApplicationsIF-IC, FC-FP

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

Website: Technical Support

(41) TFNAs induce autophagy via activation of the nonclassical cGAS–STING signalling pathway to attenuate acute lung injury in miceBMC Pulmonary MedicineNovember 7, 2025Chenru Wei, Yingying Liu, Bohan Pan, Jin Zhang, Jiqiu Chen, Ping Ling, Yang Xiang, Shihui Zhu, Yu Sun= 1:200). After washing with PBS, the nuclei were labelled with 4,6-diamino-2-phenyl-indole (DAPI; 4083, CST, US; DAPI/PBS = 1:500). Images were acquired via a fluorescence microscope (Leica, German). Flow cytometry

(42) ISG20 Restricts BK Polyomavirus Infection and Engages in Reciprocal Regulation with Viral Large T AntigenMicroorganismsNovember 6, 2025Yumin Hou, Chunlan Hu, Yejing Shi, Xiaohui Zhou, Tongyu Zhu, Nannan Wuanti-mouse antibody (Invitrogen, #A10037). Nuclei were counterstained with DAPI (Cell Signaling Technology, #4083) for 10 min before mounting. Images were acquired using a Tissue FAXS 200 flow-type tissue cytometer

(43) Methotrexate alleviates chronic inflammation in a Drosophila modelJournal of Cell ScienceNovember 1, 2025Dushyant K. Gautam, Willem Buys, Zeeshan Ahmad, Ravi K. Gutti, Indira Paddibhatlathree washes, the samples were counterstained for 15 min at room temperature with the nuclear dye DAPI (4083, 2 µg/ml, CST) or Hoechst 33342 (4082, 2.5 µg/ml, CST) as the final step for staining procedure. Final

(44) TH1 effector CD4 T cells rely on IFN-γ production to induce alopecia areataSci AdvOctober 31, 2025Samuel J. Connell, Sydney Crotts, Ryan Reis, Maddison M. Lensing, Payton Kahl, Nicholas Henderson, Otgonzaya Ayush, Zhaowen Zhu, Luana S. Ortolan, Audrey C. Ko, et al.of nuclei, sections were stained using 4′,6-diamidino-2-phenylindole (DAPI; 1 μg/ml; Cell Signaling, 4083) for 5 min at room temperature. Antifade mountant with DAPI (Thermo Fisher Scientific, P36962) was used

(45) SIK2-mediated phosphorylation of GABARAPL2 facilitates autophagosome-lysosome fusion and rescues neurodegeneration in an Alzheimer's disease modelTransl NeurodegenerOctober 23, 2025Xiaoman Dai, Ziling Ye, Chen Wang, Yufei Huang, Yun Chen, Tianqing Han, Weijie Gao, Xin Wu, Jing Zhang, Xiaochun Chenapplied overnight at 4 °C. After washes, Alexa Fluor-conjugated secondary antibodies and DAPI (CST, #4083, Danvers, MA) were applied for 10 min at RT. Sections were mounted with ProLong Gold Antifade Mountant

(46) Tuning viscoelasticity of dynamic covalent hydrogels for human tissue modelingbioRxivOctober 22, 2025Narelli de Paiva de Paiva Narciso, Fotis Christakopoulos, Michelle S. Huang, Neil J. Baugh, Carla Huerta-López, Eliana X. Matos, Kristine P. Pashin, Andrew Spakowitz, Sarah C. HeilshornFluor 555 (1:500, Invitrogen, A-21422). 4’,6-diamidino-2-phenylindole (DAPI; 1μg/mL, Cell Signaling, 4083s) was included to stain for nuclei. Huang et al. (2025b) Secondary antibodies were incubated overnight

(47) Larp1 supports brain growth and spatial memory via post-transcriptional control of the translation machinerybioRxivOctober 14, 2025Mark S. Williams, Maegan J. Watson, Carson C. Thoreen800CW,926-32211,LI-COR Bio,WB 1:5000 Anti-Rabbit HRP Conjugate,7074S,Cell Signaling,WB 1:5000 DAPI,4083S,Cell Signaling,1 μg/mL Antibodies used in this study.

(48) Integration of Primary Murine Osteocytes for In Vitro Mechanobiology StudiesCurr ProtocOctober 8, 2025Kimberly Seaman, Chun-Yu Lin, Xin Song, Amel Sassi, William W. Du, Yu Sun, Burton Yang, Lidan Yousecondary antibody solution (see recipe)4',6‐diamidino‐2‐phenylindole (DAPI) (Cell Signaling Technology, 4083) Biosafety cabinet (Thermo Scientific, Model 1355 Class A2)Aspirator (Gast, 0523‐101Q‐G180DX)Cell culture

(49) Reproducible generation of Nipah virus pseudovirions with uniform incorporation of F and G surface glycoproteins for high-throughput neutralization assaysBMC MicrobiolOctober 8, 2025Geetu Rose Varghese, Vivek Vijay, Sreeja Sreedevi, Santhik S. Lupitha, Priya Prabhakaran, Sushama Aswathyraj, Anitha P. Moorkoth, Niyas K. Pulloor, Easwaran SreekumarCat. # 8889 S, CST, USA) respectively at 1:1000 dilution. DAPI (Cell Signalling Technology, Cat. # 4083 S, CST, MA, USA) (1 µg/ml) was used as the nuclear stain in all experiments. Production of NiV pseudovirions NiV

(50) Cholesterol 25‐Hydroxylase Enhances Myeloid‐Derived Suppressor Cell (MDSC) Immunosuppression via the Stimulator of Interferon Genes (STING)‐Tank‐Binding Kinase 1 (TBK1)‐Receptor‐Interacting Protein Kinase 3 (RIPK3) Pathway in Colorectal CancerMedComm (2020)October 1, 2025Dongqin Zhou, Yu Chen, Xudong Liu, Juan He, Luyao Shen, Yongpeng He, Jiangang Zhang, Yu Zhou, Nan Zhang, Yanquan Xu, et al.sections were stained with 4′,6‐diamidino‐2′‐phenylindole (DAPI; Cell Signaling Technology, Catalogue No. 4083S) antifade reagent and visualized using a Leica fluorescence confocal microscope. For frozen section

(51) Zinc Promotes Mitochondrial Health Through PGC-1alpha Enhancing Bacterial Clearance in Macrophages Infected with Mycobacterium avium ComplexInternational Journal of Molecular SciencesSeptember 23, 2025Ruxana T. Sadikot, Prabagaran Narayanasamy, Zhihong Yuan, Deandra Smith, Daren L. KnoellT668), MitoSOX Red (Invitrogen, M36007) 4’, 6-diamidino-2-phenylindole, dihydrochloride (DAPI) (CST, 4083), Hoechst 33342 (CST, 4082). Unless otherwise specified, reagents and chemicals were obtained through

(52) AARS1-mediated lactylation of H3K18 and STAT1 promotes ferroptosis in diabetic nephropathyCell Death & DifferentiationSeptember 23, 2025Jia Hong, Hongjiao Xu, Lang Yu, Zhuang Yu, Xiangyuan Chen, Zhipeng Meng, Jiali Zhu, Jinbao Li, Minmin ZhuChina) fusion proteins were used in this study. For the IF assay, the nuclei were stained with DAPI (4083S; Cell Signaling Technology, MA, USA), and the endoplasmic reticulum was stained with ER-Tracker (C1041S,

(53) Epigenome and transcriptome changes in KMT2D-related Kabuki syndrome Type 1 iPSCs, neuronal progenitors and cortical neuronsPLOS GeneticsSeptember 19, 2025Sara Cuvertino, Evgenii Martirosian, Kedar Bhosale, Peiwen Cheng, Terence Garner, Ian J. Donaldson, Adam Jackson, Adam Stevens, Andrew D. Sharrocks, Susan J. Kimber, et al.at room temperature in the dark. After washing three times with PBS, samples were stained with DAPI (4083S, Cell signalling technology) for 5 minutes. Finally, samples were washed with PBS. Pictures were taken

(54) Biogenic Synthesis of Gold Nanoparticles Using Scabiosa palaestina Extract: Characterization, Anticancer and Antioxidant ActivitiesNanomaterials (Basel)September 4, 2025Heba Hellany, Adnan Badran, Ghosoon Albahri, Nadine Kafrouny, Riham El Kurdi, Marc Maresca, Digambara Patra, Elias Baydounmorphological changes, cells were stained with 4′,6-diamidino-2-phenylindole (DAPI) (Cell Signaling #4083, Massachusetts, USA). For this, cells were seeded in 12-well plates and treated with the indicated concentrations

(55) Autism-related proteins form a complex to maintain the striatal asymmetry in miceCell ResearchSeptember 2, 2025Yisheng Jiang, Feipeng Zhu, Jie Zhong, Xiaomei Sun, Yuting Yuan, Shuo Wang, Haiyang Chen, Zhiheng Xuappropriate fluorescent secondary antibodies (1:2000; Invitrogen) and DAPI (Cell Signaling Technology, Cat# 4083s) at RT for 1 h, followed by PBST washing for 10 min three times. Fluorescent images were captured using

(56) Mechanosensitive Channels Mediate Pancreatic β Cells Reactive Oxygen Species Formation and Downregulation of Essential Genes During Therapeutic Ultrasound TreatmentJournal of Ultrasound in MedicineSeptember 1, 2025Mallory Brayer, Vesna Zderic, Aleksandar Jeremicat room temperature for 1 hour. Cells were then incubated with DAPI (Cell Signaling catalog number 4083) in PBS for 20 minutes prior to imaging. Images were taken using a Zeiss LSCM-800 confocal microscope

(57) Mandragora autumnalis: Phytochemical Composition, Antioxidant and Anti-Cancerous Bioactivities on Triple-Negative Breast Cancer CellsInternational Journal of Molecular SciencesSeptember 1, 2025Ghosoon Albahri, Adnan Badran, Heba Hellany, Serine Baydoun, Rola Abdallah, Mohamad Alame, Akram Hijazi, Marc Maresca, Elias Baydounμg/mL). Following the manufacturer’s instructions, the cells were stained with DAPI (Cell Signaling #4083), fixed with 4% formaldehyde, and then fluorescence microscopy was used to visualize the apoptotic morphological

(58) Green Synthesis of Gold Nanoparticles Using Mandragora autumnalis: Characterization and Evaluation of Its Antioxidant and Anticancer BioactivitiesPharmaceuticals (Basel)August 29, 2025Ghosoon Albahri, Adnan Badran, Heba Hellany, Nadine Kafrouny, Riham El Kurdi, Mohamad Alame, Akram Hijazi, Marc Maresca, Digambara Patra, Elias Baydounmorphology were identified using 4′, 6-diamidino-2-phenylindole, dihydrochloride (DAPI) (Cell Signaling #4083) staining. For 24 h, cells were cultured in 12-well plates with or without the specified MAE-AuNP concentrations.

(59) Reprogramming of Fatty Acid Metabolism via PPARα‐Orchestrated FADS2 in Keratinocytes Modulates Skin Inflammation in PsoriasisAdvanced ScienceAugust 29, 2025Jiangluyi Cai, Xue Zhou, Yu Zhuang, Lian Cui, Rui Ma, Youdong Chen, Nan Yang, Qianyu Chen, Yuanyuan Wang, Peiyao Zhu, et al.the sections according to the manufacturer's instructions. The sections were then stained with DAPI (#4083, Cell Signaling Technology). Images were captured using an Olympus fluorescence microscope. Signals

(60) Development of NAFLD‐Specific Human Liver Organoid Models on a Microengineered Array Chip for Semaglutide Efficacy EvaluationCell ProliferationAugust 27, 2025Xiao‐yan You, Xiang‐yang Li, Hui Wang, Guo‐ping Zhao4412s/8889s/4408s; Beyotime, A0502) for 1 h at 25°C. Nuclei were counterstained with 1 μg/mL DAPI (Cell Signalling, 4083) for 10 min. Slides were mounted in 50% glycerol/DPBS (v/v) and imaged using a DM5000B fluorescence microscope

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