DAPI from Cell Signaling Technology

Product Specs
ItemDAPI
CompanyCell Signaling Technology
Price $74.00 Inquire
Catalog Number4083S
Quantity1 mg
Research AreaRelated Products
ApplicationsIF-IC, FC-FP

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(21) Targeting adipocyte ESRRA alleviates osteoarthritis via interrupting inter-organelle crosstalk of complement C3-CFD-MAC cascadeBone ResApril 29, 2026Tongling Huang, Zihui Wang, Lu Gao, Jun Gao, Zhaocheng Lu, Pengda Li, Chon Him Choy, Zhuolei Yuan, Yanting Zhong, Chang-An Geng, et al.Goat Anti-Rabbit IgG H&L (Alexa Fluor 555) (Abcam #ab150078). Nuclei were counterstained with DAPI (Cell Signaling Technology #4083). After two washes with PBS, sections were dried and mounted with an anti-fade reagent (Invitrogen #P10144).

(22) The PPTC7/BNIP3/NIX axis induces cGAS/STING-mediated senescence and augments CAR-T efficacy by repressing tumor-intrinsic mitophagyJournal of Experimental & Clinical Cancer ResearchApril 18, 2026Kuai Yu, Xinxin Xiong, Bo Lu, Yaqun Wang, Tiantian Sun, Mei Xie, Xiaojun Xu, Yuanbin Song, Wei XiaoAbcam, UK) was applied for 1 h at room temperature, followed by nuclear counterstaining with DAPI (#4083, Cell Signaling Technology, USA) for 10 min. Images were acquired using a confocal microscope (Dragonfly,

(23) IDH2 lactylation regulates mitochondrial dysfunction injury induced by myocardial ischemia‑reperfusion via the AMPK signaling pathwayInt J Mol MedApril 17, 2026Changsen Wang, Siman Shen, Suyun Chen, Jinda Lin, Simeng Li, Jianning Chen, Shuyun Cai, Xiaodong Yuan, Ziqiang Yang, Kun Ding, et al.dilution of 1:500 for 1 h at room temperature in the dark. Nuclei were counterstained with DAPI (cat. no. 4083S; Cell Signaling Technology, Inc.; diluted to 5×10−4 mg/ml) for 5 min. Slides were mounted with antifade

(24) Mono- and co-infections of primary porcine respiratory cells with Bordetella bronchiseptica and Streptococcus suis are not affected by the dermonecrotic toxinInfection and immunityApril 15, 2026Schaaf D, Dresen M, Weldearegay YB, Biermann J, Brockmeier SL, Baumgärtner W, Jarek M, Valentin-Weigand P.were stained with 4′,6-diamidino-2-phenylindole (DAPI, 0.5 μg/mL; Cell Signaling Technology, Cat. No. 4083). Finally, the membrane was cut out and mounted with ProLong Gold Antifade Reagent (Cell Signaling Technology,

(25) Accumulation of mutations in nsp4, E, and the S2 subunit underlies mammalian cell tropism expansion and virulence attenuation of avian coronavirusPLoS PathogApril 9, 2026Yingfei Li, Xuehui Zhang, Rong Liang, Ruotong Li, Ruihua Yang, Liwei Zhang, Ye Zhao, Guozhong Zhang, Jing Zhaotemperature. Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Cell Signaling Technology, #4083) for 10 min at room temperature. Fluorescence images were acquired using a Nikon microscope. Western blot

(26) GV1001 Reprograms CD47 Immune Checkpoint to Restore Macrophage Antitumor Activity in Oral Squamous Cell CarcinomaInt J Mol SciApril 7, 2026Wei Chen, Seojin Kim, Cheyenne Beheshtian, Angela Jun, Sangjae Kim, No-Hee Parkcat. no. A11007) for 1 h. Nuclei were counterstained with DAPI (Cell Signaling Technology, cat. no. 4083, Danvers, MA, USA). Fluorescent images were acquired using a confocal laser-scanning microscope (LSM

(27) An Activity‐Dependent NEPAS–PTX3 Axis Links Neurovascular and Myelin Deficits to Cognitive ImpairmentAdvanced ScienceApril 7, 2026Boya Hu, Zifei Chen, Bingmei Sun, Jiale Gao, Jiale Xu, Xiaochun Guo, Fenfei Gao, Zhongsi Wang, Jie Wu, Xiaoyu Ji, et al.secondary antibodies (Jackson ImmunoResearch). 4′, 6‐Diamidino‐2‐phenylindole (DAPI) (1:106, CST, #4083) was used to stain the nuclei. The sections were then washed and visualized under an LSM 800 laser confocal

(28) Inhibition of adipocyte RUNX1/2 enhances adipose tissue thermogenesis through distinct mechanismsNature CommunicationsApril 2, 2026Cuihua Wang, Na He, Shixiang Wang, Miao Lei, Jie Yao, Li Lin, Xue Ding, Jiaman Lin, Yixin Chen, Qiaoyun Long, et al.antibodies at room temperature for 1 h. Nuclei were counterstained with DAPI (Cell Signaling Technology, 4083). For immunofluorescence staining of tissue cryosections, adipose tissues were fixed in 4% paraformaldehyde

(29) TIA1-mediated stress granules inhibit the neuroinflammation and neurodegeneration after spinal cord injury through LCN2 signalingCellular and Molecular Life SciencesMarch 31, 2026Mengxian Jia, Ziwei Fan, Yaozhi He, Yumin Wu, Jian Zhou, Jiawei Wang, Yuhao Luo, Xiaowu Lin, Jiashu Lian, Zhoule Zhu, et al.Novus), goat anti-Iba1 (1:500, ab5076, Abcam). Additionally, the nucleus was marked with DAPI (1:1, 000, #4083, CST). Images were captured using a confocal laser scanning microscope (FV3000RS, Olympus) or a fluorescence microscope

(30) YAP1 and QSER1 are key modulators of embryonic signaling pathways in the mammalian epiblastEMBO ReportsMarch 26, 2026Elizabeth Abraham, Thomas Roule, Aidan Douglas, Emily Megill, Olivia M Pericak, Jordan E Howe, Carmen Choya-Foces, Joanne F Garbincius, Henry M Cohen, Paula Roig-Flórez, et al.,Thermo Fisher Scientific,A-31573 Mouse anti-Beta-catenin,Santa Cruz,sc-7963 DAPI,Cell Signaling Technologies,4083S Rabbit anti-BRACHYURY,Cell Signaling Technologies,D2Z37 Mouse anti-Gapdh,Protein Tech,60004-1 Mouse

(31) Ursodeoxycholic Acid Attenuates Lipopolysaccharide-Induced Myocardial Injury by Inhibiting Oxidative Stress, Inflammation, and Apoptosis: The Interplay of Sirt1/Nrf2 and Akt/NF-κB Signaling PathwaysInt J Mol SciMarch 20, 2026Ranko Škrbić, Tatjana Milivojac, Milkica Grabež, Ljiljana Amidžić, Zorislava Bajic, Tanja Sobot, Nebojša Mandić-Kovačević, Snežana Uletilović, Đorđe Đukanović, Milica Gajic Bojic, et al.temperature. Nuclei were stained with 4′,6-diamidino-2-phenylindole ((DAPI), Cell Signaling Technology, 4083, Danvers, MA, USA) and imaged using a fluorescent microscope (Leica DM 6000B, Wetzlar, Germany). Fluorescence

(32) Parvalbumin-positive neurons in the medial septum participate in the formation of hippocampal-dependent spatial memoryNature CommunicationsMarch 20, 2026Yawen Zheng, Jifu Tong, Yuanwei Xing, Shan Shao, Naizheng Liu, Shuting Liu, Jiao Wu, Wang Yin, Jiaxin Wang, Shulu Yuan, et al.Fluor 647-conjugated goat anti-rabbit IgG (1:500, ab150075, Abcam), counterstained with DAPI (1:1000, 4083S, CST), and imaged with a confocal microscope (Olympus FV1000). Cell numbers were quantified using Image-Pro

(33) IRX4204 sensitizes multiple myeloma to ferroptosis and improves lenalidomide efficacy through the HMOX1-GPX4 axisScientific ReportsMarch 17, 2026Jian Wu, Zhibo Yan, Kimberly Burcher, Zhannan Han, Mikhail A. Nikiforov, Vidyasagar Vuligonda, Martin Sanders, Yubin Kanganti-rabbit secondary antibody (Thermo Fisher, R37117). Nuclei were stained with DAPI (Cell Signaling 4083) and coverslipped with antifade medium (Vector Laboratories). Images were obtained using a Leica SP5

(34) KAT2B-mediated epigenetic suppression of RAD51C enhances olaparib sensitivity in colorectal cancerCancer Chemother PharmacolMarch 14, 2026Xiaoqin Liu, Jun Chen, Xuwei Shen, Lin Liwas followed by a 20-minute staining process in which DAPI (from Cell Signalling, catalogue number 4083 S) was used to visualize the nuclei. Finally, the coverslips were washed and mounted with anti-fade

(35) Adhesion‐Related Macrophages Regulate Metabolic Homeostasis Through CAV‐1 DependencyAdvanced ScienceMarch 12, 2026Wanyu Hu, Xiyan Liao, Limin Xie, Xiaoxiao Sun, Luodi Jing, Qin Zeng, Hongwei Jiang, Haowei Zhang, Lei Li, Fanqi Wang, et al.propidium iodide (1:200, 421301, Biolegend), Zombie NIR (1:100, 423106, Biolegend), or DAPI (0.2 µg/mL, 4083, Cell Signaling Technology). For CAV‐1 flow cytometry analysis, live cells were first blocked with CD16/32

(36) Investigating the Effect and Mechanism of Protocatechuic Aldehyde on Vascular Dementia Based on Multi-Omics ApproachBiomoleculesMarch 11, 2026Tian Xiao, Jie Tao, Jiaoyang Tan, Xingjie Yi, Ziyi Wang, Xiaohua DuanPA, USA), MCT4, GFAP, NeuN (22787-1-AP, 60190-1-Ig, 26975-1-AP, Proteintech, Chicago, IL, USA), DAPI (4083, Cell Signaling Technology, Danvers, MA, USA), dilution ratio 1:200, and incubated at 4 °C overnight.

(37) MSTN and TCF12 as Candidate Immunometabolic Signatures in Glioma-Associated Foam Cells: Insights from Integrated Multi-Omics AnalysisCurr Issues Mol BiolMarch 9, 2026Xu Liu, Zhuo Song, Zhijia Sun, Chen Liu, Xiaoli Kang, Huilian Qiao, Xinzhuo Tu, Teng Li, Zhiguang Fu, Yingjie Wang5220-0336 and 5220-0341, MA, USA). Nuclei were counterstained with DAPI (1:4000; Cell Signaling Technology, 4083, MA, USA). Images were acquired using a Zeiss Axio Scope A1 Fluorescence Microscope (Carl Zeiss AG,

(38) Microglia Pyroptosis-Derived IL-18 Drives White Matter Injury in Developing Brain following Hypothermic Hypoxia-IschemiaNeuroscience BulletinMarch 9, 2026Hongtong Chen, Shengyu Jin, Mingdong Liu, Yifan Zhu, Liren Zhang, Cong Li, Peng Liu, Xiaoping Tong, Zhongqun ZhuRRID: AB_11180865). Brain sections were counterstained with DAPI (1:1000, Cell Signaling, Danvers, USA, 4083S) for 10 min at room temperature before mounting with Fluoromount™ aqueous medium (13800, AQUA-MOUNT,

(39) Early life adversity impairs visually evoked innate defensive behaviors via oxytocin signalingCommunications BiologyFebruary 21, 2026Huiying Tan, Junying Su, Shaohua Ma, Shanping Chen, Qingqing Liu, Xing Yang, Liping Wangfor Oxtr staining. Finally, the sections were incubated in 1‰ DEPC-treated PBS with DAPI (1:5000, Cat#4083S, Cell Signaling Technology, USA). Imaging was performed using a virtual microscopy slide-scanning system

(40) Transcriptomic and metabolomic insights into gabapentin’s therapeutic role in neurogenic inflammation of rosaceaCommunications BiologyFebruary 14, 2026Ziqi Jiang, Tian Ding, Yan Zhao, Mao Luo, Yishu Tang, Yuxuan Zhang, Bin Weiwith PBS again. Finally, the samples were mounted with an anti-fade mounting medium containing DAPI (4083, CST) or Hoechst (4082, CST), and images were acquired and observed under a laser scanning confocal

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DAPI

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