DAPI from Cell Signaling Technology

Product Specs
ItemDAPI
CompanyCell Signaling Technology
Price $74.00 Inquire
Catalog Number4083S
Quantity1 mg
Research AreaRelated Products
ApplicationsIF-IC, FC-FP

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(21) Activating Ras-MAPK pathway variants drive hippocampal clonal competition in human epilepsybioRxivJanuary 30, 2026Sattar Khoshkhoo, Mingyun Bae, Yilan Wang, Ashton Tillett, Rosita B. Ramirez, Benjamin Finander, Emily D. Egan, Linus Marx, Dipan Patel, Zinan Zhou, et al.donkey anti-mouse IgG (H+L) Alexa Fluor 488 (1:500, Thermo Fisher A21202) for two hours at room temperature. DAPI (1:1,000, Cell Signaling #4083) was included during secondary incubation for nuclear counterstaining. Slides were washed five times

(22) PU.1 inhibition sensitizes stem-monocytic AML to BCL2 blockadebioRxivJanuary 28, 2026William M Yashar, Itallia V Pacentine, Akram Taherinasab, Thai Nguyen, Samantha Worme, Mitsuhiro Tsuchiya, Theresa Lusardi, Chelsea Hutchinson, Thomas L Fillmore, Camilo Posso, et al.Eppendorf ThermoMixer C. Tagmented nuclei were pooled, stained with DAPI (Cell Signaling Technologies #4083), and sorted into a 96-well plate using a Sony SH800S cell sorter. Nuclear proteins were denatured by

(23) PDX1 phosphorylation at S61 by mTORC1 links nutrient signaling to β cell function and metabolic diseaseCell ReportsJanuary 27, 2026Jialin Fan, Xin Zhang, Jinling Zhang, Tinghan Zhao, Stephen K. Burley, X.F. Steven Zhengadhered to the manufacturer’s instructions. Cells or tissue slides were subsequently incubated with DAPI (4083S, Cell Signaling Technology) for 15 min and mounted with fluorescence mounting medium (0100-20, SouthernBiotech).

(24) 1,3-and 1,4-linked polysaccharides uptake in intestinal cells relies on clathrin/dynamin 1/Rab5-dependent endocytosisNature CommunicationsJanuary 26, 2026Wenfeng Liao, Dianxiu Cao, Ying Wang, Zhenyun Du, Jian Yao, Pengfei Dou, Yuandong Zheng, Zhiming Wang, Xia Chen, Peipei Wang, et al.h. Then, the cells were fixed with 4% paraformaldehyde for 15 min followed by staining with DAPI (#4083, Cell Signaling Technology, USA) and scanning using confocal microscopy (FV 1000, Olympus, Japan; Lecia

(25) Targeting sorting nexin 3 to treat pulmonary fibrosis by dual modulating Wnt/β-catenin signalingCell Death & DiseaseJanuary 15, 2026Dinghu Ma, Wenjing Yu, Hang Zhou, Rongyuan Lin, Ximeng Sun, Mingxia Peng, Chenjia Lin, Haoyu Du, Yueqi Li, Huimin Liang, et al.#8889S, #8890S) for visualization. The cell nucleus was stained with DAPI (CST, USA, diluted 1:1000, #4083). Images were captured using an FV3000 fluorescence microscope (Olympus, Japan). Colocalization analysis

(26) IL-27 signaling mediates skin inflammation in experimental psoriasis and atopic dermatitisCell & BioscienceJanuary 7, 2026Zeyu Chen, Lian Cui, Zhiyi Lan, Suyang Lin, Nan Yang, Siqi Li, Zihan Zhao, Jiangluyi Cai, Yuanyuan Wang, Tong Liu, et al.manufacturer’s instructions, then nuclei were stained with DAPI (1:1000, Cell Signaling Technology, Cat# 4083). Finally, the fluorescence-stained sections were mounted with ProLong Gold Antifade reagent and visualized

(27) Establishment of a human inner ear model reveals that gentamicin C2b is substantially less ototoxic than clinical gentamicinBiomedicine & PharmacotherapyJanuary 6, 2026Minjin Jeong, Hiroaki Mohri, Dongjun Han, Masaharu Sakagami, Konstantina M. StankovicIgG (Invitrogen, A10042; 1:500). Nuclei were stained with 0.1 μg/mL DAPI (Cell Signaling Technology, 4083) and samples were mounted using Vectashield (Vector Laboratories, H1000). Imaging was performed using

(28) Normal cardiac lymphatics and their mimicsAmerican Journal of Physiology-Heart and Circulatory PhysiologyJanuary 1, 2026Sarah A. Ware, Lirong Zheng, Milad Almasian, Lorenzo Fernandes, Chitkale Hiremath, Jonathan A. Brewer, Zhi-Jing Wu, Claudia Munoz Muñoz Rodríguez, Shuyue Zhou, Denise K. Marciano, et al.slides were washed 3×5 min then incubated with secondary antibodies and DAPI (1 mg/mL, diluted 1:2000; 4083, Cell Signaling Technology) diluted in PBS for 30 min. The sections were washed 3×5 min, mounted with

(29) Dileucine-supplemented essential amino acids support whole-body anabolism after resistance exercise and serum-stimulated cell-based anabolismJ Int Soc Sports NutrDecember 31, 2025Jonathan A. Aguilera, Cassidy T. Tinline-Goodfellow, Matthew J. Lees, Ines Kortebi, Daniel W. D. West, Sidney Abou Sawan, Megha Sharma, Raza Bashir, Takeshi M. Barnes, Alexander V. Ulanov, et al.BSA/DPBS in the dark. The cells were then rinsed once in DPBS and incubated with DAPI (1:5,000 in PBS; #4083; Cell Signaling Technology) for 5 min in the dark. Finally, the cells were rinsed twice in DPBS, stored

(30) Notum1a Inhibition promotes neurogenesis in the adult zebrafish brainScientific ReportsDecember 26, 2025Yigit Kocagöz, Nuray Sogunmez Erdogan, Sevval Ozdinc, Dogac Ipekgil, Esra Katkat, Gunes OzhanJackson Immunoresearch Laboratories). Nuclear staining was performed with 4′,6-diamidino-2-phenylindole (DAPI; 4083 S, Cell Signaling Technology). Quantitative PCR (qPCR) analysis qPCR reactions were performed in quadruples

(31) Resolving forebrain developmental organisation by analysis of differential growth patternsNature CommunicationsDecember 21, 2025Elizabeth Manning, Kavitha Chinnaiya, Caitlyn Furley, Dong Won Kim, Seth Blackshaw, Marysia Placzek, Elsie Placenecessary. Tissue was counterstained with DAPI prior to imaging (Cell Signaling Technology, Cat no. 4083, 1:1000). Quantification of DiI/DiO results Seventeen DiI/DiO outcome areas (Fig. 1H), and the growth

(32) Creation of a rich vascular subcutaneous space for cell transplantation via injectable biological hydrogelsSci RepDecember 20, 2025Asmaa Samy, Ghada A. El-Sherbeny, Sherry M. Khater, Haytham G. Aamer, Mohamed A. Abdelhameed, Ayman F. Refaie#4408, Cell Signaling Technology) and the nuclear counterstain 4′,6-diamidino-2-phenylindole (DAPI; #4083, Cell Signaling Technology) were also used for immunofluorescence imaging. Preparation of hydrogels

(33) α-Parvin regulation of cell re-arrangement is critical for ureteric bud branching morphogenesisbioRxivDecember 8, 2025Xinyu Dong, Fabian Bock, Ali Hashmi, Nada Bulus, Glenda Mernaugh, Gema Bolas, Shensen Li, Wanying Zhu, Meiling Melzer, Kyle Brown, et al.anti-SIX2,Proteintech,11562-1-AP Laminin,MilliporeSigma,L9393 Rhodamine-conjugated phalloidin,ThermoFisher,R415 DAPI,Cell Signaling,4083 Ki67,abcam,ab15580 Rabbit anti-α-Parvin Sepharose Beads,Cell Signaling,12591 Rabbit monoclonal anti-ILK

(34) Human pluripotent stem cell-derived skin organoids enabled pathophysiological model of Mycobacterium tuberculosis infectionNat CommunDecember 2, 2025Liang Yue, Yating Liang, Pengfei Zhong, Leiming Fang, Zhimin Yun, Qisheng Su, Chaofan Li, Meida Xiang, Jie Zhang, Jiru Wang, et al.room temperature protected from light. After repeating the previous washing steps, DAPI staining (#4083, Cell Signaling) was added in DPBS for 15 min at room temperature in the dark. Finally, samples were

(35) Pathological significance and therapeutic prospects of HCCS expression patterns in lung adenocarcinomaCell adhesion & migrationDecember 1, 2025Luo P, Wu S, Li Z, Tan S, Zeng L, Tang Y, Luo L, Li Y, Tang Z.fluorescent marker for simultaneous detection of both markers. Nuclei were stained with DAPI (Catalog No. 4083, CST) for 8 minutes. Finally, fluorescently labeled cells were imaged using a fluorescence microscope,

(36) Thymosin β4 stabilizes hypoxia induced brain microvascular endothelial cell dysfunction through S1PR1 dependent mechanismsSci RepDecember 1, 2025William G. Stewart, Christina D. Hejl, Rakeshwar S. Guleria, Sudhiranjan Guptawere captured by Leica DMi8 Imaging system. Anti-S1PR1 (cat. no. 63335, lot no.1) and DAPI (cat. no. 4083) were purchased from Cell Signaling Technology, Inc., USA. Western blotting The protein lysates were

(37) Follicle-like niches outside the cortex? 3D phase-contrast µCT revealed medullary B cell nodules in mucosa-draining lymph nodesFrontiers in ImmunologyNovember 19, 2025Paul Schütz, Florian L. Schwarzenberg, Lennert J. Weber, Jörg U. Hammel, Bente Siebels, Paula Nissen, Nenya Leising, Katja J. Jarick, Bernd Walkenfort, Sarah C. Irvine, et al.3x 10 min in 0.1% PBST and incubated with DAPI as a nuclear counterstain (Cell Signaling, Cat. No.: 4083S, Cambridge, UK) 1:1000 in PBS for 2 hours. For stainings using unconjugated (primary) antibodies, secondary

(38) KIF11 variants in familial exudative vitreoretinopathy leading to mTORC1 overactivation and impaired cell cycle progressionHuman GenomicsNovember 19, 2025Min Liu, Shengliu Pan, Erkuan Dai, Rulian ZhaoTechnology), rabbit anti-p21 (10355-1-AP, Proteintech), rabbit anti-CDK1 (19532-1-AP, Proteintech), DAPI (4083, Cell Signaling Technology), Hoechst (CM03826, Proteintech), anti-mouse IgG (H + L), Alexa Fluor Plus

(39) Pinostrobin Inhibits Nuclear Factor-Kappa B Signaling and Production of Inflammatory Cytokines and Chemokines in Human MacrophagesNutrientsNovember 17, 2025Phatcharaporn Budluang, Saranyapin Potikanond, Nitwara Wikan, Wutigri Nimlamoolgoat anti-mouse IgG conjugated with Alexa488 (both from Thermo Fisher Scientific) and DAPI (Cat No. 4083) (Cell Signaling Technology) for detecting the cell nuclei. The coverslips were mounted onto a fluorescent microscope

(40) Barium titanate piezoelectric nanoparticles induce M1 polarization in mouse macrophages via ultrasound in vitroScientific ReportsNovember 12, 2025Timothy Connolly, Camille Johnson, Allison Chen, Krzysztof Kempa, Dylan Hatt, Michael J. Naughtonin PBS. A 5 μg ml−1 solution of 4′,6-diamidino-2-phenylindole (DAPI; Cell Signaling Technology, no. 4083) was then added to the cells for 10 min. The DAPI stain was removed and cells were washed 3 times with

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