DAPI from Cell Signaling Technology

Product Specs
ItemDAPI
CompanyCell Signaling Technology
Price $74.00 Inquire
Catalog Number4083S
Quantity1 mg
Research AreaRelated Products
ApplicationsIF-IC, FC-FP

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

Website: Technical Support

(21) Early life adversity impairs visually evoked innate defensive behaviors via oxytocin signalingCommunications BiologyFebruary 21, 2026Huiying Tan, Junying Su, Shaohua Ma, Shanping Chen, Qingqing Liu, Xing Yang, Liping Wangfor Oxtr staining. Finally, the sections were incubated in 1‰ DEPC-treated PBS with DAPI (1:5000, Cat#4083S, Cell Signaling Technology, USA). Imaging was performed using a virtual microscopy slide-scanning system

(22) Transcriptomic and metabolomic insights into gabapentin’s therapeutic role in neurogenic inflammation of rosaceaCommunications BiologyFebruary 14, 2026Ziqi Jiang, Tian Ding, Yan Zhao, Mao Luo, Yishu Tang, Yuxuan Zhang, Bin Weiwith PBS again. Finally, the samples were mounted with an anti-fade mounting medium containing DAPI (4083, CST) or Hoechst (4082, CST), and images were acquired and observed under a laser scanning confocal

(23) Human lung organoids model for assessing host response to Mycobacterium tuberculosis infectionFrontiers in Cellular and Infection MicrobiologyFebruary 10, 2026Chaofan Li, Pengfei Zhong, Zhimin Yun, Leiming Fang, Meida Xiang, Qisheng Su, Jiru Wang, Hebing Chen, Zhi Chen, Liang Yue, et al.(Thermo Scientific) for 1 hour in the dark, and counterstained with DAPI (Cell Signaling Technology, 4083S) for 15 minutes. Finally, the sections were mounted with an anti-fade mounting medium (Dako, S3023)

(24) Triple targeting of STING, TGF-β, and PD-L1 boosts CXCL16-CXCR6 signaling for potent antitumor responseNat CommunFebruary 9, 2026Ming Yi, Tianye Li, Yinhui Gu, Mengke Niu, Dixuan Xue, Shengtao Hu, Yuze Wu, Bin Zhao, Di Zhang, Yingkang Ma, et al.used for multiplex IF are listed in Table S2. After staining, sections were counterstained with DAPI (4083, CST), mounted with an antifade reagent, and imaged using a confocal microscope. Image analysis was

(25) Kaempferol Alleviates Glucocorticoid-Induced Osteonecrosis of the Femoral Head by Modulating Macrophage M1/M2 Polarization Through RhoA/ROCK-Mediated Mitophagy ActivationBiomedicinesFebruary 9, 2026Yuankai Zhang, Yan Zhao, Shangqing Zhang, Tian Lei, Bocheng Xiang, Xin Zhang, Kai Nan, Lihong Fanwere introduced for 2 h at ambient temperature. Subsequently, tissue sections received DAPI staining (4083S, CST). Protein expression levels were then assessed via laser confocal microscopy. 2.10. Immunohistochemical Staining

(26) Nanoplastics Impair GnRH Neuron Migration and Neuroendocrine Function: Emerging Players in the Pathogenesis of Reproductive DisordersSmallFebruary 6, 2026Federica Amoruso, Alyssa Julia Jennifer Paganoni, Astrid Saraceni, Andrea Magnani, Alessia Brossa, Giorgio Roberto Merlo, Cristina Matei, Ruben Heinjan Willemsen, Raíssa Carneiro Rezende, Alexander Augusto de Lima Jorge, et al.counterstained DAPI (4′,6‐diamidin‐2‐phenylindole, 1:10,000; Cell Signaling, Leiden, Netherlands; cat. 4083). To detect F‐actin filaments, cells were stained with TRITC‐conjugated phalloidin (1:400; Merck, Darmstadt,

(27) Formyl peptide receptor 2 activation by MR-39 inhibits glioblastoma cell proliferation and invasiveness through suppression of multiple oncogenic pathwaysJournal of Translational MedicineFebruary 6, 2026Maria Grazia Ferraro, Marco Bocchetti, Marianna Affinito, Ercolano Giuseppe, Kardelen Dalim Filiz, Luisa Speranza, Enza Lacivita, Marcello Leopoldo, Marianna Crispino, Anna Ceccarelli, et al.Cell Signaling #4412). In both cases, nuclei were counterstained with DAPI (1:10000, Cell Signaling #4083), and fluorescence images were acquired using a confocal microscope (Zeiss LSM 900 Airyscan 2) with

(28) Integrin-Mediated Mechanosensing of Modeled Lymph Node Microenvironment Promotes T Cell Activation via Nuclear DeformationResearch (Wash D C)February 6, 2026jinteng feng, Guoqing Zhao, Lingzhu Zhao, Luying Geng, Shirong Zhang, Longwen Xu, Mengjie Liu, Guangjian Zhang, Feng Xu, Min Lin, et al.rhodamine phalloidin (1:500; R415, Invitrogen, USA). 4′,6-Diamidino-2-phenylindole (DAPI; 0.5 μg/ml; 4083, Cell Signaling Technology, USA) was used to stain cell nuclei. For enzyme-linked immunosorbent assay

(29) Kindlins regulate integrin- and growth factor-dependent ureteric bud formationDevelopmentFebruary 1, 2026Shensen Li, Fabian Bock, Olga Viquez, Anjana Hassan, Sijo Mathew, Riya Palamuttam, Glenda Mernaugh, Xinyu Dong, Meiling Melzer, Matthew Tantengco, et al.Alexa Fluor 555 anti-rat (ThermoFisher, 78945; 1:200) secondary antibodies and DAPI (Cell Signaling, 4083) were used subsequently. Immunofluorescence images were taken on a Zeiss LSM 980 confocal microscope

(30) Activating Ras-MAPK pathway variants drive hippocampal clonal competition in human epilepsybioRxivJanuary 30, 2026Sattar Khoshkhoo, Mingyun Bae, Yilan Wang, Ashton Tillett, Rosita B. Ramirez, Benjamin Finander, Emily D. Egan, Linus Marx, Dipan Patel, Zinan Zhou, et al.donkey anti-mouse IgG (H+L) Alexa Fluor 488 (1:500, Thermo Fisher A21202) for two hours at room temperature. DAPI (1:1,000, Cell Signaling #4083) was included during secondary incubation for nuclear counterstaining. Slides were washed five times

(31) PU.1 inhibition sensitizes stem-monocytic AML to BCL2 blockadebioRxivJanuary 28, 2026William M Yashar, Itallia V Pacentine, Akram Taherinasab, Thai Nguyen, Samantha Worme, Mitsuhiro Tsuchiya, Theresa Lusardi, Chelsea Hutchinson, Thomas L Fillmore, Camilo Posso, et al.Eppendorf ThermoMixer C. Tagmented nuclei were pooled, stained with DAPI (Cell Signaling Technologies #4083), and sorted into a 96-well plate using a Sony SH800S cell sorter. Nuclear proteins were denatured by

(32) PDX1 phosphorylation at S61 by mTORC1 links nutrient signaling to β cell function and metabolic diseaseCell ReportsJanuary 27, 2026Jialin Fan, Xin Zhang, Jinling Zhang, Tinghan Zhao, Stephen K. Burley, X.F. Steven Zhengadhered to the manufacturer’s instructions. Cells or tissue slides were subsequently incubated with DAPI (4083S, Cell Signaling Technology) for 15 min and mounted with fluorescence mounting medium (0100-20, SouthernBiotech).

(33) 1,3-and 1,4-linked polysaccharides uptake in intestinal cells relies on clathrin/dynamin 1/Rab5-dependent endocytosisNature CommunicationsJanuary 26, 2026Wenfeng Liao, Dianxiu Cao, Ying Wang, Zhenyun Du, Jian Yao, Pengfei Dou, Yuandong Zheng, Zhiming Wang, Xia Chen, Peipei Wang, et al.h. Then, the cells were fixed with 4% paraformaldehyde for 15 min followed by staining with DAPI (#4083, Cell Signaling Technology, USA) and scanning using confocal microscopy (FV 1000, Olympus, Japan; Lecia

(34) Targeting sorting nexin 3 to treat pulmonary fibrosis by dual modulating Wnt/β-catenin signalingCell Death & DiseaseJanuary 15, 2026Dinghu Ma, Wenjing Yu, Hang Zhou, Rongyuan Lin, Ximeng Sun, Mingxia Peng, Chenjia Lin, Haoyu Du, Yueqi Li, Huimin Liang, et al.#8889S, #8890S) for visualization. The cell nucleus was stained with DAPI (CST, USA, diluted 1:1000, #4083). Images were captured using an FV3000 fluorescence microscope (Olympus, Japan). Colocalization analysis

(35) IL-27 signaling mediates skin inflammation in experimental psoriasis and atopic dermatitisCell & BioscienceJanuary 7, 2026Zeyu Chen, Lian Cui, Zhiyi Lan, Suyang Lin, Nan Yang, Siqi Li, Zihan Zhao, Jiangluyi Cai, Yuanyuan Wang, Tong Liu, et al.manufacturer’s instructions, then nuclei were stained with DAPI (1:1000, Cell Signaling Technology, Cat# 4083). Finally, the fluorescence-stained sections were mounted with ProLong Gold Antifade reagent and visualized

(36) Establishment of a human inner ear model reveals that gentamicin C2b is substantially less ototoxic than clinical gentamicinBiomedicine & PharmacotherapyJanuary 6, 2026Minjin Jeong, Hiroaki Mohri, Dongjun Han, Masaharu Sakagami, Konstantina M. StankovicIgG (Invitrogen, A10042; 1:500). Nuclei were stained with 0.1 μg/mL DAPI (Cell Signaling Technology, 4083) and samples were mounted using Vectashield (Vector Laboratories, H1000). Imaging was performed using

(37) Normal cardiac lymphatics and their mimicsAmerican Journal of Physiology-Heart and Circulatory PhysiologyJanuary 1, 2026Sarah A. Ware, Lirong Zheng, Milad Almasian, Lorenzo Fernandes, Chitkale Hiremath, Jonathan A. Brewer, Zhi-Jing Wu, Claudia Munoz Muñoz Rodríguez, Shuyue Zhou, Denise K. Marciano, et al.slides were washed 3×5 min then incubated with secondary antibodies and DAPI (1 mg/mL, diluted 1:2000; 4083, Cell Signaling Technology) diluted in PBS for 30 min. The sections were washed 3×5 min, mounted with

(38) Dileucine-supplemented essential amino acids support whole-body anabolism after resistance exercise and serum-stimulated cell-based anabolismJ Int Soc Sports NutrDecember 31, 2025Jonathan A. Aguilera, Cassidy T. Tinline-Goodfellow, Matthew J. Lees, Ines Kortebi, Daniel W. D. West, Sidney Abou Sawan, Megha Sharma, Raza Bashir, Takeshi M. Barnes, Alexander V. Ulanov, et al.BSA/DPBS in the dark. The cells were then rinsed once in DPBS and incubated with DAPI (1:5,000 in PBS; #4083; Cell Signaling Technology) for 5 min in the dark. Finally, the cells were rinsed twice in DPBS, stored

(39) Notum1a Inhibition promotes neurogenesis in the adult zebrafish brainScientific ReportsDecember 26, 2025Yigit Kocagöz, Nuray Sogunmez Erdogan, Sevval Ozdinc, Dogac Ipekgil, Esra Katkat, Gunes OzhanJackson Immunoresearch Laboratories). Nuclear staining was performed with 4′,6-diamidino-2-phenylindole (DAPI; 4083 S, Cell Signaling Technology). Quantitative PCR (qPCR) analysis qPCR reactions were performed in quadruples

(40) Resolving forebrain developmental organisation by analysis of differential growth patternsNature CommunicationsDecember 21, 2025Elizabeth Manning, Kavitha Chinnaiya, Caitlyn Furley, Dong Won Kim, Seth Blackshaw, Marysia Placzek, Elsie Placenecessary. Tissue was counterstained with DAPI prior to imaging (Cell Signaling Technology, Cat no. 4083, 1:1000). Quantification of DiI/DiO results Seventeen DiI/DiO outcome areas (Fig. 1H), and the growth

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