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Chromatography Equipment
Chromatography and Separation
MojoSort™ Mouse CD45 Nanobeads
MojoSort™ Mouse CD45 Nanobeads from BioLegend
(2)
Write a review
Citations:
(28)
Supplier Page
Supplier Page from
BioLegend for
MojoSort™ Mouse CD45 Nanobeads
Description
MojoSort™ Mouse CD45 Nanobeads
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
MojoSort™ Mouse CD45 Nanobeads
Company
BioLegend
Price
$694.00
Supplier Page
View Company Product Page
Catalog Number
480028
Quantity
200 tests
Applications
Cell Separation (MojoSort™, ) - Quality tested
Format
Aqueous solution containing BSA and 0.05% sodium azide
Type
Beads
Species
Mouse
Target
CD45
Add to Compare List
BioLegend
8999 BioLegend Way
San Diego
,
California
92121
United States
Phone
: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587
Company Profile
Website:
biolegend.com
Citations (28)
(1)
Single-cell lineage tracing identifies hemogenic endothelial cells in the adult mouse bone marrow
eLife
April 27, 2026
Jing-Xin Feng, Mei-Ting Yang, Lili Li, Caiyi C Li, Ferenc Livák, Jack Chen, Yongmei Zhao, Dunrui Wang, Avinash Bhandoola, Naomi Taylor,
et al.
blocking, BM cells were first depleted of
CD45
+ cells with
MojoSort mouse CD45 nanobeads
(
BioLegend
#
480028
) following the manufacturer’s protocol and then stained with specific antibodies.
For flow cytometry
(2)
Early-life stromal niches orchestrate B lymphopoiesis at the brain's borders
bioRxiv
November 30, 2025
Alec J. Walker, Theodore M. Fisher, Sydney K. Caldwell, Anushree S. Gupte, Justin M. Colville-Reimertz, Bristy Sabikunnahar, Samuel E. Marsh, Yvanka de Soysa, Vahid H. Gazestani, Alicia C. Walker,
et al.
cells using the Biolegend MojoSort magnet (
Biolegend
,
480020
) and mouse CD45 nanobeads (
Biolegend
,
480028
). Separation was completed following the manufacturer’s instructions with magnets kept on ice to preserve
(3)
TRIM63/IRF-8 axis promotes tumor progression and immunosuppression of melanoma with BRAF mutation
Cell Death & Disease
November 28, 2025
Fei Yi, Shuotong Liu, Yan Ma, Ye Li, Banchen Chen, Bo Yu
LTD (Cincinnati, OH, USA). FITC anti-
CD45
(
103107
), PE anti-CD8 (140408), APC anti-
GZMB
(
396407
,) and
MojoSort™ Mouse CD45 Nanobeads
(
480027
) were purchased from
Biolegend
(San Diego, CA, USA). Anti-Flag M2 Affinity Gels (
A2220
), streptavidin
(4)
CCL5hi Macrophages Interact with CD8+ T Cells and Potentiate Responsiveness to PD‐1 Blockade Plus Chemotherapy in Esophageal Squamous Cell Carcinoma
Advanced Science
September 25, 2025
Yuanzhen Ma, Xiaodong Su, Tingting Zeng, Chunlian Zhou, Guozhen Yang, Zengfei Xia, Chufeng Zeng, Jiarong Zhan, Xin‐Yuan Guan, Xu Zhang,
et al.
Data Generation and Processing
Mouse
tumor
tissues were isolated, and CD45+ cells were sorted with
MojoSort™ Mouse CD45 Nanobeads
Kit (
Biolegend
, San Diego, CA). Single‐cell cellular suspensions were subjected to
sequencing
in 10K
(5)
Paracrine regulations of IFN-γ secreting CD4+ T cells by lumican and biglycan are protective in allergic contact dermatitis
Matrix Biology
September 1, 2025
George Maiti, Jihane Frikeche, Cynthia Loomis, Michael Cammer, Stephanie L Eichman, Shukti Chakravarti
filtered through 100 μm cell strainer and enriched for CD45− cells by removing the CD45+ cells using the
MojoSort mouse CD45 nanobeads
kit (
Biolegend
). Cells were then stained with Live-Dead Blue, CD45, CD31, and PDPN and sorted for the
(6)
Transplants foster B cell alloimmunity by relaying extracellular vesicles to follicular dendritic cells
Cell reports
June 24, 2025
Chen R, Powell JS, Shufesky WJ, Bardhi E, Sullivan MLG, Tkacheva OA, Camirand G, Goncalves Z, Stolz DB, Chalasani G,
et al
.
"
CD14 microbeads, human
",
Miltenyi Biotec
,Cat #
130-050-201
MojoSort™
Mouse CD45 Nanobeads
,
BioLegend
,Cat #
480028
EasySep™ Biotin Positive Positive Selection Kitt II,
Stemcell Technologies
,Cat #
17683
Magnet,Stemcell
(7)
Activation of HTR2B Suppresses Osteosarcoma Progression through the STAT1‐NLRP3 Inflammasome Pathway and Promotes OASL1+ Macrophage Production to Enhance Antitumor Immunity
Advanced Science
May 19, 2025
Zhen Huang, Jiazhuang Zhu, Jianping Hu, Xingkai Wang, Xiaolong Ma, Enjie Xu, Kunpeng Zhu, Chunlin Zhang
processed according to standard protocols: minced, washed, and dissociated into single cells using
MojoSort Mouse CD45 Nanobeads
(
BioLegend
, USA). Single‐cell suspensions were analyzed using the 10K Genomics‐PerseusTM according to
(8)
Application of imaging mass cytometry for spatially profiling the microenvironment of salivary glands in primary Sjögren's syndrome
Cell Death Dis
May 16, 2025
Guolin Wu, Fangping Wu, Lipei Wang, Lixiong Ying, Wenwen Lu, Kang Qian, Tianxiao Fu, Danbin Wu, Fenglin Hu, YiHang Shi,
et al.
cells were further isolated from the cell suspension by magnetic-activated
cell separation
(MASC) using
MojoSort™ Mouse CD45 Nanobeads
(#
480027
,
Biolegend
, USA), and then were analyzed by FCM after stained by anti-
CD8
(#
100705
,
Biolegend
,
(9)
Single-cell transcriptomics reveals a compartmentalized antiviral interferon response in the nasal epithelium of mice
Journal of Virology
March 18, 2025
Xuefei Wang, Meng Dong, Xinchao Wu, Daniel Schnepf, Julia Thiel, Wenfei Sun, Christian Wolfrum, Sisi Li, Wenfei Jin, Peter Staeheli,
et al.
suspensions derived from snouts were depleted of CD45+ using
Mojosort mouse CD45 nanobeads
(
BioLegend
,
480028
) and magnets according to the manufacturer’s instructions. In brief, snout single cells were washed
(10)
Mapping cell diversity and dynamics in inflammatory temporomandibular joint osteoarthritis with pain at single-cell resolution
JCI Insight
February 10, 2025
Supawadee Jariyasakulroj, Yang Shu, Ziying Lin, Jingyi Chen, Qing Chang, Pao-Fen Ko, Jian-Fu Chen
and centrifuged at 300g for 10 minutes at 4°C. Immune cell depletion was performed as described in
MojoSort Mouse CD45 Nanobeads
Protocol – Depletion (
BioLegend
). Briefly, samples from the cell dissociation step were washed and resuspended
(11)
Blockade of CCR5+ T Cell Accumulation in the Tumor Microenvironment Optimizes Anti‐TGF‐β/PD‐L1 Bispecific Antibody
Advanced Science
November 1, 2024
Ming Yi, Tianye Li, Mengke Niu, Yuze Wu, Bin Zhao, Zhuoyang Shen, Shengtao Hu, Chaomei Zhang, Xiaojun Zhang, Jing Zhang,
et al.
1 mg mL−1 Collagenase B) at 37 °C for 40 min. Subsequently, nanobeads for CD45 positive selection (
480028
,
BioLegend
) were utilized to isolate sufficient immune cells (≈80%) for
scRNA‐seq
. Living cells were
(12)
The Wdr5-H3K4me3 Epigenetic Axis Regulates Pancreatic Tumor Immunogenicity and Immune Suppression
International Journal of Molecular Sciences
August 12, 2024
Kaidi Deng, Liyan Liang, Yingcui Yang, Yanmin Wu, Yan Li, Rongrong Zhang, Yulin Tian, Chunwan Lu
immune cells were then purified from the
tumor
cell suspension using a Mouse CD45 Selection Kit (Cat#
480028
,
BioLegend
, San Diego, CA, USA) and separated by a magnetic stand. 4.13. WDR5-47 Synthesis WDR5-47
(13)
Stroke and myocardial infarction induce neutrophil extracellular trap release disrupting lymphoid organ structure and immunoglobulin secretion
Nat Cardiovasc Res
May 1, 2024
Tuz AA, Ghosh S, Karsch L, Ttoouli D, Sata SP, Ulusoy Ö, Kraus A, Hoerenbaum N, Wolf JN, Lohmann S,
et al
.
further purified using direct B
cell labeling
with CD45 microbeads using
MojoSort Mouse CD45 Nanobeads
(
480028
,
BioLegend
). This isolation
procedure
provides B cells with a purity of 99% or greater. Afterwards,
(14)
A lipid-associated macrophage lineage rewires the spatial landscape of adipose tissue in early obesity
JCI Insight
October 9, 2023
Cooper M. Stansbury, Gabrielle A. Dotson, Harrison Pugh, Alnawaz Rehemtulla, Indika Rajapakse, Lindsey A. Muir
strainers and centrifuged at 300g at 4°C to pellet SVCs. SVCs were enriched for CD45+ immune cells using
MojoSort Mouse CD45 Nanobeads
(
Biolegend
; catalog
480027
) following the manufacturer’s protocol. Briefly, SVC pellets were resuspended
(15)
FRC transplantation restores lymph node conduit defects in laminin α4–deficient mice
JCI Insight
April 24, 2023
Lushen Li, Long Wu, Allison Kensiski, Jing Zhao, Marina W. Shirkey, Yang Song, Wenji Piao, Tianshu Zhang, Zhongcheng Mei, Samuel J. Gavzy,
et al.
12-week-old female C57BL/6
mice
for enzymatic digestion.
MojoSort Mouse CD45 Nanobeads
(
BioLegend
, catalog
480028
) were used to select for CD45– LNSCs following the manufacturer’s instructions. Viable CD45– LNSCs were
(16)
HnRNPA2B1 Aggravates Inflammation by Promoting M1 Macrophage Polarization
Nutrients
March 23, 2023
Meiyao Meng, Yuxiang Cao, Yankang Zhang, Shuang Liu, Yinzhao Zhong, Dongmei Wang, Dali Li, Lingyan Xu, Xinran Ma
APC anti-mouse CD45
, PE anti-PPmouse CD140a (PDGF Receptor a). Immune cells were firstly isolated using
mouse CD45+ nanobeads
(
Biolegend
, San Diego, CA, USA).
Flow cytometry
data were collected using Fortessa (BD LSR Fortessa,
(17)
PDGFRα‐lineage origin directs monocytes to trafficking proficiency to support peripheral immunity
European Journal of Immunology
February 1, 2022
Yu‐Tung Li, Sho Yamazaki, Eiichi Takaki, Yuya Ouchi, Tomomi Kitayama, Katsuto Tamai
anti‐CD64‐APC (X54‐5/7.1). For
staining
of colonic cells, antibody
staining
was accompanied by addition of
mouse CD45 nanobeads
(
Biolegend
) for magnetic enrichment of
leukocytes
from colonic digest. Cells, if not fixed, were stained
(18)
DNA hypomethylation leads to cGAS-induced autoinflammation in the epidermis
The EMBO journal
November 15, 2021
Beck MA, Fischer H, Grabner LM, Groffics T, Winter M, Tangermann S, Meischel T, Zaussinger-Haas B, Wagner P, Fischer C,
et al
.
min at 37°C. After
filtration
using a 70‐µm cell strainer,
CD45
‐positive cells were depleted using
Mojosort Mouse CD45 Nanobeads
(
480027
,
BioLegend
) according to manufacturer’s instructions. Purity of epidermal
keratinocytes
was
(19)
Invariant Natural Killer T cells coordinate removal of senescent cells
Med
August 13, 2021
Arora S, Thompson PJ, Wang Y, Bhattacharyya A, Apostolopoulou H, Hatano R, Naikawadi RP, Shah A, Wolters PJ, Koliwad S,
et al
.
REAGENT or RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies
CD45
nanobeads,
BioLegend
,
480028
Anti-mouse Cd45 APC (
Clone
30F-11),
BioLegend
,
103112
Purified anti-mouse CD16/32
(2.4G2) antibody,UCSF
(20)
Def6 regulates endogenous type-I interferon responses in osteoblasts and suppresses osteogenesis
eLife
December 29, 2020
Deng Z, Ng C, Inoue K, Chen Z, Xia Y, Hu X, Greenblatt M, Pernis A, Zhao B.
All Subtype ELISA Kit and VeriKine-HS Mouse IFN Beta ELISA Kit were purchased from PBL Assay Science.
MojoSort Mouse CD45 Nanobeads
were obtained from
Biolegend
.
Cell culture
In vitro
osteoblast
differentiation: primary osteoblastic
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Reviews
Write a Review
(1)
5/7/2018
Good Depletion Kit
Jingxin Feng
The general aim is to explore some rare cells in bone marrow that are CD45-. CD45 depletion was performed to remove most cells unwanted. The reason this product was selected was because it was half ...
Read Review
(1)
9/18/2017
Isolation Of Mouse CD45- Cells (Negative Selection)
Jeeho Lee
The purpose of using this kit is to enrich non-immune cells (CD45-) by depleting CD45+ cells.
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MojoSort™ Mouse CD45 Nanobeads from BioLegend
Supplier Page
View Company Product Page
$694.00
Product Specs
Company Info
Citations
Reviews
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