MojoSort™ Mouse CD45 Nanobeads from BioLegend

Product Specs
ItemMojoSort™ Mouse CD45 Nanobeads
CompanyBioLegend
Price $694.00Supplier Page View Company Product Page
Catalog Number480028
Quantity200 tests
ApplicationsCell Separation (MojoSort™, ) - Quality tested
FormatAqueous solution containing BSA and 0.05% sodium azide
TypeBeads
SpeciesMouse
TargetCD45

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Single-cell lineage tracing identifies hemogenic endothelial cells in the adult mouse bone marroweLifeApril 27, 2026Jing-Xin Feng, Mei-Ting Yang, Lili Li, Caiyi C Li, Ferenc Livák, Jack Chen, Yongmei Zhao, Dunrui Wang, Avinash Bhandoola, Naomi Taylor, et al.blocking, BM cells were first depleted of CD45+ cells with MojoSort mouse CD45 nanobeads (BioLegend #480028) following the manufacturer’s protocol and then stained with specific antibodies. For flow cytometry

(2) Early-life stromal niches orchestrate B lymphopoiesis at the brain's bordersbioRxivNovember 30, 2025Alec J. Walker, Theodore M. Fisher, Sydney K. Caldwell, Anushree S. Gupte, Justin M. Colville-Reimertz, Bristy Sabikunnahar, Samuel E. Marsh, Yvanka de Soysa, Vahid H. Gazestani, Alicia C. Walker, et al.cells using the Biolegend MojoSort magnet (Biolegend, 480020) and mouse CD45 nanobeads (Biolegend, 480028). Separation was completed following the manufacturer’s instructions with magnets kept on ice to preserve

(3) TRIM63/IRF-8 axis promotes tumor progression and immunosuppression of melanoma with BRAF mutationCell Death & DiseaseNovember 28, 2025Fei Yi, Shuotong Liu, Yan Ma, Ye Li, Banchen Chen, Bo YuLTD (Cincinnati, OH, USA). FITC anti-CD45 (103107), PE anti-CD8 (140408), APC anti-GZMB (396407,) and MojoSort™ Mouse CD45 Nanobeads (480027) were purchased from Biolegend (San Diego, CA, USA). Anti-Flag M2 Affinity Gels (A2220), streptavidin

(4) CCL5hi Macrophages Interact with CD8+ T Cells and Potentiate Responsiveness to PD‐1 Blockade Plus Chemotherapy in Esophageal Squamous Cell CarcinomaAdvanced ScienceSeptember 25, 2025Yuanzhen Ma, Xiaodong Su, Tingting Zeng, Chunlian Zhou, Guozhen Yang, Zengfei Xia, Chufeng Zeng, Jiarong Zhan, Xin‐Yuan Guan, Xu Zhang, et al.Data Generation and Processing Mouse tumor tissues were isolated, and CD45+ cells were sorted with MojoSort™ Mouse CD45 Nanobeads Kit (Biolegend, San Diego, CA). Single‐cell cellular suspensions were subjected to sequencing in 10K

(5) Paracrine regulations of IFN-γ secreting CD4+ T cells by lumican and biglycan are protective in allergic contact dermatitisMatrix BiologySeptember 1, 2025George Maiti, Jihane Frikeche, Cynthia Loomis, Michael Cammer, Stephanie L Eichman, Shukti Chakravartifiltered through 100 μm cell strainer and enriched for CD45− cells by removing the CD45+ cells using the MojoSort mouse CD45 nanobeads kit (Biolegend). Cells were then stained with Live-Dead Blue, CD45, CD31, and PDPN and sorted for the

(6) Transplants foster B cell alloimmunity by relaying extracellular vesicles to follicular dendritic cellsCell reportsJune 24, 2025Chen R, Powell JS, Shufesky WJ, Bardhi E, Sullivan MLG, Tkacheva OA, Camirand G, Goncalves Z, Stolz DB, Chalasani G, et al."CD14 microbeads, human",Miltenyi Biotec,Cat # 130-050-201 MojoSort™ Mouse CD45 Nanobeads,BioLegend,Cat # 480028 EasySep™ Biotin Positive Positive Selection Kitt II,Stemcell Technologies,Cat # 17683 Magnet,Stemcell

(7) Activation of HTR2B Suppresses Osteosarcoma Progression through the STAT1‐NLRP3 Inflammasome Pathway and Promotes OASL1+ Macrophage Production to Enhance Antitumor ImmunityAdvanced ScienceMay 19, 2025Zhen Huang, Jiazhuang Zhu, Jianping Hu, Xingkai Wang, Xiaolong Ma, Enjie Xu, Kunpeng Zhu, Chunlin Zhangprocessed according to standard protocols: minced, washed, and dissociated into single cells using MojoSort Mouse CD45 Nanobeads (BioLegend, USA). Single‐cell suspensions were analyzed using the 10K Genomics‐PerseusTM according to

(8) Application of imaging mass cytometry for spatially profiling the microenvironment of salivary glands in primary Sjögren's syndromeCell Death DisMay 16, 2025Guolin Wu, Fangping Wu, Lipei Wang, Lixiong Ying, Wenwen Lu, Kang Qian, Tianxiao Fu, Danbin Wu, Fenglin Hu, YiHang Shi, et al.cells were further isolated from the cell suspension by magnetic-activated cell separation (MASC) using MojoSort™ Mouse CD45 Nanobeads (#480027, Biolegend, USA), and then were analyzed by FCM after stained by anti-CD8 (#100705, Biolegend,

(9) Single-cell transcriptomics reveals a compartmentalized antiviral interferon response in the nasal epithelium of miceJournal of VirologyMarch 18, 2025Xuefei Wang, Meng Dong, Xinchao Wu, Daniel Schnepf, Julia Thiel, Wenfei Sun, Christian Wolfrum, Sisi Li, Wenfei Jin, Peter Staeheli, et al.suspensions derived from snouts were depleted of CD45+ using Mojosort mouse CD45 nanobeads (BioLegend, 480028) and magnets according to the manufacturer’s instructions. In brief, snout single cells were washed

(10) Mapping cell diversity and dynamics in inflammatory temporomandibular joint osteoarthritis with pain at single-cell resolutionJCI InsightFebruary 10, 2025Supawadee Jariyasakulroj, Yang Shu, Ziying Lin, Jingyi Chen, Qing Chang, Pao-Fen Ko, Jian-Fu Chenand centrifuged at 300g for 10 minutes at 4°C. Immune cell depletion was performed as described in MojoSort Mouse CD45 Nanobeads Protocol – Depletion (BioLegend). Briefly, samples from the cell dissociation step were washed and resuspended

(11) Blockade of CCR5+ T Cell Accumulation in the Tumor Microenvironment Optimizes Anti‐TGF‐β/PD‐L1 Bispecific AntibodyAdvanced ScienceNovember 1, 2024Ming Yi, Tianye Li, Mengke Niu, Yuze Wu, Bin Zhao, Zhuoyang Shen, Shengtao Hu, Chaomei Zhang, Xiaojun Zhang, Jing Zhang, et al.1 mg mL−1 Collagenase B) at 37 °C for 40 min. Subsequently, nanobeads for CD45 positive selection (480028, BioLegend) were utilized to isolate sufficient immune cells (≈80%) for scRNA‐seq. Living cells were

(12) The Wdr5-H3K4me3 Epigenetic Axis Regulates Pancreatic Tumor Immunogenicity and Immune SuppressionInternational Journal of Molecular SciencesAugust 12, 2024Kaidi Deng, Liyan Liang, Yingcui Yang, Yanmin Wu, Yan Li, Rongrong Zhang, Yulin Tian, Chunwan Luimmune cells were then purified from the tumor cell suspension using a Mouse CD45 Selection Kit (Cat# 480028, BioLegend, San Diego, CA, USA) and separated by a magnetic stand. 4.13. WDR5-47 Synthesis WDR5-47

(13) Stroke and myocardial infarction induce neutrophil extracellular trap release disrupting lymphoid organ structure and immunoglobulin secretionNat Cardiovasc ResMay 1, 2024Tuz AA, Ghosh S, Karsch L, Ttoouli D, Sata SP, Ulusoy Ö, Kraus A, Hoerenbaum N, Wolf JN, Lohmann S, et al.further purified using direct B cell labeling with CD45 microbeads using MojoSort Mouse CD45 Nanobeads (480028, BioLegend). This isolation procedure provides B cells with a purity of 99% or greater. Afterwards,

(14) A lipid-associated macrophage lineage rewires the spatial landscape of adipose tissue in early obesityJCI InsightOctober 9, 2023Cooper M. Stansbury, Gabrielle A. Dotson, Harrison Pugh, Alnawaz Rehemtulla, Indika Rajapakse, Lindsey A. Muirstrainers and centrifuged at 300g at 4°C to pellet SVCs. SVCs were enriched for CD45+ immune cells using MojoSort Mouse CD45 Nanobeads (Biolegend; catalog 480027) following the manufacturer’s protocol. Briefly, SVC pellets were resuspended

(15) FRC transplantation restores lymph node conduit defects in laminin α4–deficient miceJCI InsightApril 24, 2023Lushen Li, Long Wu, Allison Kensiski, Jing Zhao, Marina W. Shirkey, Yang Song, Wenji Piao, Tianshu Zhang, Zhongcheng Mei, Samuel J. Gavzy, et al.12-week-old female C57BL/6 mice for enzymatic digestion. MojoSort Mouse CD45 Nanobeads (BioLegend, catalog 480028) were used to select for CD45– LNSCs following the manufacturer’s instructions. Viable CD45– LNSCs were

(16) HnRNPA2B1 Aggravates Inflammation by Promoting M1 Macrophage PolarizationNutrientsMarch 23, 2023Meiyao Meng, Yuxiang Cao, Yankang Zhang, Shuang Liu, Yinzhao Zhong, Dongmei Wang, Dali Li, Lingyan Xu, Xinran MaAPC anti-mouse CD45, PE anti-PPmouse CD140a (PDGF Receptor a). Immune cells were firstly isolated using mouse CD45+ nanobeads (Biolegend, San Diego, CA, USA). Flow cytometry data were collected using Fortessa (BD LSR Fortessa,

(17) PDGFRα‐lineage origin directs monocytes to trafficking proficiency to support peripheral immunityEuropean Journal of ImmunologyFebruary 1, 2022Yu‐Tung Li, Sho Yamazaki, Eiichi Takaki, Yuya Ouchi, Tomomi Kitayama, Katsuto Tamaianti‐CD64‐APC (X54‐5/7.1). For staining of colonic cells, antibody staining was accompanied by addition of mouse CD45 nanobeads (Biolegend) for magnetic enrichment of leukocytes from colonic digest. Cells, if not fixed, were stained

(18) DNA hypomethylation leads to cGAS-induced autoinflammation in the epidermisThe EMBO journalNovember 15, 2021Beck MA, Fischer H, Grabner LM, Groffics T, Winter M, Tangermann S, Meischel T, Zaussinger-Haas B, Wagner P, Fischer C, et al.min at 37°C. After filtration using a 70‐µm cell strainer, CD45‐positive cells were depleted using Mojosort Mouse CD45 Nanobeads (480027, BioLegend) according to manufacturer’s instructions. Purity of epidermal keratinocytes was

(19) Invariant Natural Killer T cells coordinate removal of senescent cellsMedAugust 13, 2021Arora S, Thompson PJ, Wang Y, Bhattacharyya A, Apostolopoulou H, Hatano R, Naikawadi RP, Shah A, Wolters PJ, Koliwad S, et al.REAGENT or RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies CD45 nanobeads,BioLegend,480028 Anti-mouse Cd45 APC (Clone 30F-11),BioLegend,103112 Purified anti-mouse CD16/32 (2.4G2) antibody,UCSF

(20) Def6 regulates endogenous type-I interferon responses in osteoblasts and suppresses osteogenesiseLifeDecember 29, 2020Deng Z, Ng C, Inoue K, Chen Z, Xia Y, Hu X, Greenblatt M, Pernis A, Zhao B.All Subtype ELISA Kit and VeriKine-HS Mouse IFN Beta ELISA Kit were purchased from PBL Assay Science. MojoSort Mouse CD45 Nanobeads were obtained from Biolegend. Cell culture In vitro osteoblast differentiation: primary osteoblastic

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(1)

5/7/2018Good Depletion Kit Jingxin FengThe general aim is to explore some rare cells in bone marrow that are CD45-. CD45 depletion was performed to remove most cells unwanted. The reason this product was selected was because it was half ... Read Review
(1)

9/18/2017Isolation Of Mouse CD45- Cells (Negative Selection) Jeeho LeeThe purpose of using this kit is to enrich non-immune cells (CD45-) by depleting CD45+ cells.

MojoSort™ Mouse CD45 Nanobeads from BioLegend

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MojoSort™ Mouse CD45 Nanobeads from BioLegend

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