ProtoScript II Reverse Transcriptase M0368L from New England Biolabs

Product Specs
ItemProtoScript II Reverse Transcriptase
CompanyNew England Biolabs
Price $198.00 Inquire
Catalog NumberM0368L
Size10000 units
ApplicationsDNA Analysis
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(61) SIKs Regulate HDAC7 Stabilization and Cytokine Recall in Late-Stage T Cell Effector DifferentiationJournal of immunology (Baltimore, Md. : 1950)December 15, 2023Helms RS, Marin-Gonzalez A, Patel CH, Sun IH, Wen J, Leone RD, Duvall B, Gao RD, Ha T, Tsukamoto T, et al.equivalent amounts of cDNA were generated per condition using ProtoScript II Reverse Transcriptase (NEB Cat# M0368L) and random hexamers (Qiagen Cat# 79236) according to manufacturer’s protocol. qRT-PCR for Ifng (ThermoFisher Scientific

(62) A rapid, multiplex digital PCR assay to detect gene variants and fusions in non‐small cell lung cancerMolecular OncologyNovember 1, 2023Bryan Leatham, Katie McNall, Hari K. K. Subramanian, Lucien Jacky, John Alvarado, Dominic Yurk, Mimi Wang, Donald C. Green, Gregory J. Tsongalis, Aditya Rajagopal, et al.Thermo Fisher Scientific), 2.4 μL 5× primer–probe mix (Table S5), 0.6 μL reverse transcriptase (PN M0368S; New England Biolabs, Ipswich, MA, USA), 5 μL RNA sample (1–3 ng total RNA), and 1.6 μL 1× TE Buffer

(63) Fluoroquinolone and beta-lactam antimicrobials induce different transcriptome profiles in Salmonella enterica persister cellsScientific ReportsOctober 31, 2023S. P. Mattiello, V. C. Barth, J. Scaria, C. A. S. Ferreira, S. D. Oliveira(dT23VN) (New England BioLabs, S1330S) and ProtoScript II Reverse Transcriptase (New England BioLabs, M0368L), followed by synthesis of the second-strand cDNA (dsDNA) using the NEBNext Ultra II Non-directional

(64) Transcriptome-wide analysis of the function of Ded1 in translation preinitiation complex assembly in a reconstituted in vitro systembioRxivOctober 20, 2023Fujun Zhou, Julie M. Bocetti, Meizhen Hou, Daoming Qin, Alan G. Hinnebusch, Jon R. Lorschpurified for reverse transcription using RT primer NI-802 and ProtoScript II Reverse Transcriptase (NEB; M0368S). The ~105 nt cDNAs were separated from free RT primers on a 15% TBE-Urea gel and circularized using

(65) The impact of Parkinson’s disease-associated gut microbiota on the transcriptome in DrosophilaMicrobiology SpectrumOctober 17, 2023Xin Liu, Meng Yang, Runzhou Liu, Fan Zhou, Haibing Zhu, Xiaoyun Wangthe fragmented and primed mRNA was reversed into cDNA using ProtoScript II Reverse Transcriptase (#M0368S, NEB) in a 20 µL reaction with the procedure of 10 minutes at 25℃ and 15 minutes at 42℃ followed by

(66) Efficient genetic editing of human intestinal organoids using ribonucleoprotein-based CRISPRDisease Models & MechanismsOctober 1, 2023Nefeli Skoufou-Papoutsaki, Sam Adler, Paula D'Santos, Liz Mannion, Shenay Mehmed, Richard Kemp, Amy Smith, Francesca Perrone, Komal Nayak, Alasdair Russell, et al.P-value). qPCR validation cDNA conversion was performed using ProtoScript II RT standard protocol (NEB, M0368) and Random Primer Mix (NEB, S1330S). qPCR was performed using TaqMan Fast Universal PCR Master Mix

(67) Mechanisms of readthrough mitigation reveal principles of GCN1-mediated translational quality controlCellJuly 20, 2023Müller MBD, Kasturi P, Jayaraj GG, Hartl FU.instructions. Reverse transcription was performed using Protoscript II reverse transcriptase (NEB, M0368L). Ligation products were excised from a 15% TBE-urea polyacrylamide gel after hydrolyzing the RNA template

(68) Depositing centromere repeats induces heritable intragenic heterochromatin establishment and spreading in ArabidopsisNucleic acids researchJuly 7, 2023Liu ZW, Liu J, Liu F, Zhong X.300 ng total RNAs were used for reverse transcription by OligodT18VN primer and ProtoScript II (NEB, #M0368L) reverse transcriptase according to the manufacturer's instructions. Quantitative PCR was performed

(69) VarID2 quantifies gene expression noise dynamics and unveils functional heterogeneity of ageing hematopoietic stem cellsGenome BiologyJune 23, 2023Reyna Edith Rosales-Alvarez, Jasmin Rettkowski, Josip Stefan Herman, Gabrijela Dumbović, Nina Cabezas-Wallscheid, Dominic Grünwas used. For cDNA first-strand synthesis, Protoscript II and Protoscript II Reaction Buffer (NEB, M0368L) as well as murine RNase-Inhibitor (NEB, M0314S) was used instead of SuperScript II reverse transcriptase,

(70) Squid express conserved ADAR orthologs that possess novel featuresFrontiers in Genome EditingJune 5, 2023Isabel C. Vallecillo-Viejo, Gjendine Voss, Caroline B. Albertin, Noa Liscovitch-Brauer, Eli Eisenberg, Joshua J. C. RosenthalcDNA using Protoscript II Reverse Transcriptase and Oligo dT primers (New England Biolabs, Cat No. M0368). cDNA from 15 ng total RNA was then quantified using hydrolysis probe-based qPCR with primers specific

(71) Decapping factor Dcp2 controls mRNA abundance and translation to adjust metabolism and filamentation to nutrient availabilityeLifeJune 2, 2023Anil Kumar Vijjamarri, Xiao Niu, Matthew D Vandermeulen, Chisom Onu, Fan Zhang, Hongfang Qiu, Neha Gupta, Swati Gaikwad, Miriam L Greenberg, Paul J Cullen, et al.(McGlincy and Ingolia, 2017), followed by reverse transcription using Protoscript II (New England Biolabs, M0368L) and circularization of cDNA using CircLigase ssDNA Ligase (Epicenter, CL4111K). Each pooled library

(72) Systematic dissection of coordinated stromal remodeling identifies Sox10+ glial cells as a therapeutic target in myelofibrosisCell Stem CellJune 1, 2023Shawn M. Sarkaria, Junsong Zhou, Suying Bao, Wenqi Zhao, Yinshan Fang, Jianwen Que, Govind Bhagat, Chaolin Zhang, Lei Dingwas used for first-strand cDNA synthesis (New England BioLabs ProtoScript II reverse transcriptase, M0368L). Quantitative real-time PCR was carried out using GoTaq qPCR Master Mix (Promega) on a Bio-Rad CFX

(73) Molecular mechanisms of stress-induced reactivation in mumps virus condensatesCellApril 27, 2023Xiaojie Zhang, Sindhuja Sridharan, Ievgeniia Zagoriy, Christina Eugster Oegema, Cyan Ching, Tim Pflaesterer, Herman K.H. Fung, Isabelle Becher, Ina Poser, Christoph W. Müller, et al.101000027 RNeasy Micro Kit,Qiagen,Cat#74004 ProtoScript II Reverse Transcriptase Kit,New England Biolabs,Cat#M0368 "TaqPath qPCR Master Mix, CG",Applied Biosystems,Cat#A15297 RNA Nano 6000 Assay Kit,Agilent Technologies,Cat#5067-1511 NEBNext

(74) Transcriptional kinetic synergy: A complex landscape revealed by integrating modeling and synthetic biologyCell SystemsApril 19, 2023Rosa Martinez-Corral, Minhee Park, Kelly M. Biette, Dhana Friedrich, Clarissa Scholes, Ahmad S. Khalil, Jeremy Gunawardena, Angela H. DePace50-980-492 iTaq™Universal SYBR Green reagent,BioRad,Cat# 1725121 Protoscript II reverse transcriptase,New England Biolabs,M0368 oligo-dT primers,New England Biolabs,S1316 Puromycin Dihydrochloride,Gibco,Cat# A1113803 ,, Critical

(75) Bacterial drug-resistance and viability phenotyping upon disinfectant exposure revealed by single-nucleotide resolved-allele specific isothermal RNA amplificationJournal of Hazardous MaterialsApril 15, 2023Xinyu Liao, Xuhan Xia, Hao Yang, Yulin Zhu, Ruijie Deng, Tian DingThermo Fisher Scientific (Waltham, USA). RNase H (5 U/μL) (NO. M0297), ProtoScriptII reverse transcriptase (200 U/μL) (NO. M0368S), deoxynucleotide (dNTP) solution mix (10 mM) (NO. N0447S), and ribonucleotide (rNTPs) solution mix

(76) Chemical-guided SHAPE sequencing (cgSHAPE-seq) informs the binding site of RNA-degrading chimeras targeting SARS-CoV-2 5’ untranslated regionbioRxivApril 7, 2023Zhichao Tang, Shalakha Hegde, Siyuan Hao, Manikandan Selvaraju, Jianming Qiu, Jingxin Wang7.4, 2 μL), DTT (100 mM, 1 μL), dNTP (10 mM, 0.5 μL), ProtoScript II (0.5 μL, New England Biolabs, M0368L) and RNase inhibitor (0.2 μL, ApexBio, K1046) were added. The reaction was incubated at 42 °C for 1

(77) Programmable protein delivery with a bacterial contractile injection systemNatureApril 1, 2023Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F.We then generated cDNA from these bulk RNA extracts using ProtoScript II Reverse Transcriptase (NEB M0368) and random primers (NEB S1330) with the manufacturer’s protocol. Finally, we ran qPCR on the resulting

(78) The super elongation complex drives transcriptional addiction in MYCN-amplified neuroblastomaScience advancesMarch 29, 2023Wang D, Yin Z, Wang H, Wang L, Li T, Xiao R, Xie T, Han R, Dong R, Liu H, et al.#N401-02) and fragmented to 200 to 500 bp size long in 2× ProtoScript II buffer (New England Biolabs, #M0368). First- and second-strand DNA synthesis and adaptor ligation were conducted using an New England Biolabs

(79) ILF3 prion-like domain regulates gene expression and fear memory under chronic stressiScienceMarch 17, 2023Yamashita A, Shichino Y, Fujii K, Koshidaka Y, Adachi M, Sasagawa E, Mito M, Nakagawa S, Iwasaki S, Takao K, et al.I,Lucigen,Code: N6901K SUPERase In,Thermo Fisher Scientific,Cat#AM2694 ProtoScriptII,New England Biolabs,Cat#M0368L CircLigase II,Lucigen,Code: CL9021K ,, Critical commercial assays,Critical commercial assays,Critical

(80) Genomic diversity of SARS-CoV-2 can be accelerated by mutations in the nsp14 geneiScienceMarch 17, 2023Kosuke Takada, Mahoko Takahashi Ueda, Shintaro Shichinohe, Yurie Kida, Chikako Ono, Yoshiharu Matsuura, Tokiko Watanabe, So NakagawaPrimeSTAR GXL DNA polymerase,Takara,Cat# R050A ProtoScript II Reverse Transcriptase,New England Biolabs,Cat# M0368S NEBNext® Ultra™ II Non-Directional RNASecond Strand Synthesis Module,New England Biolabs,Cat# E6111S/L SureSelectXT

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ProtoScript II Reverse Transcriptase

ProtoScript II Reverse Transcriptase from New England Biolabs

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ProtoScript II Reverse Transcriptase from New England Biolabs

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