ProtoScript II Reverse Transcriptase M0368L from New England Biolabs

Product Specs
ItemProtoScript II Reverse Transcriptase
CompanyNew England Biolabs
Price $198.00 Inquire
Catalog NumberM0368L
Size10000 units
ApplicationsDNA Analysis
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(101) Mechanistic basis for maintenance of CHG DNA methylation in plantsNature communicationsJuly 5, 2022Fang J, Jiang J, Leichter SM, Liu J, Biswal M, Khudaverdyan N, Zhong X, Song J.synthesized from 2 μg of the extracted total RNA using anchored oligodT18VN and ProtoScript II (NEB, M0368) reverse transcriptase. For McrBC-qPCR, plant genomic DNA was extracted using the cetyltrimethylammonium

(102) CRISPR-Cas13a cascade-based viral RNA assay for detecting SARS-CoV-2 and its mutations in clinical samplesSensors and Actuators B: ChemicalJuly 1, 2022Yuxi Wang et al.procured from Thermo Fisher Scientific (Waltham, USA). ProtoScriptⅡ Reverse Transcriptase (200 U/μL, #M0368S), dNTPs (#N0447S) and rNTPs mixes (#N0466S) were procured from NEB (Ipswich, USA). Hifair® Ⅲ 1st Strand

(103) Persistent CAD activity in memory CD8(+) T cells supports rRNA synthesis and ribosomal biogenesis required at rechallengeScience immunologyMay 27, 2022Claiborne MD, Sengupta S, Zhao L, Arwood ML, Sun IM, Wen J, Thompson EA, Mitchell-Flack M, Laiho M, Powell JD.15596018). RNA was converted to cDNA using the ProtoScript II Reverse Transcriptase (New England BioLabs, M0368). qRT-PCR was performed with PowerSYBR® Green PCR Master Mix (ThermoFisher). 5’ ETS pre-rRNA, Ifng,

(104) Nationwide Trends in COVID-19 Cases and SARS-CoV-2 RNA Wastewater Concentrations in the United StatesACS ES&T WaterMay 3, 2022Claire Duvallet et al.quantify the RNA. First, 10 μL of RNA samples was first reverse transcribed in a 25 μL reaction (NEB M0368), and then 2 μL per reaction of cDNA was assayed by qPCR (ThermoFisher 4444557) using a SARS-CoV-2 nucleocapsid

(105) Ageing induces tissue‐specific transcriptomic changes in Caenorhabditis elegansThe EMBO JournalApril 19, 2022Xueqing Wang, Quanlong Jiang, Yuanyuan Song, Zhidong He, Hongdao Zhang, Mengjiao Song, Xiaona Zhang, Yumin Dai, Oezlem Karalay, Christoph Dieterich, et al.Protease from Streptomyces griseus,Sigma‐Aldrich,Cat# P6911 ProtoScript II Reverse Transcriptase,New England Biolabs,Cat # M0368L Q5® High‐Fidelity DNA Polymerase,New England Biolabs,Cat# M0491L RiboLock RNase Inhibitor,Thermo Fisher,Cat

(106) Akaby—Cell-free protein expression system for linear templatesPLOS ONEApril 7, 2022Wakana Sato, Judee Sharon, Christopher Deich, Nathaniel Gaut, Brock Cash, Aaron E. Engelhart, Katarzyna P. AdamalaProtoscript II Reverse Transcriptase Buffer, 1 μl of Protoscript II Reverse Transcriptase (200 U/μl, Catalog No. M0368, New England BioLabs Inc.), 2 μl of 0.1M dithiothreitol (DTT), 1 μl of 10mM dNTP, 0.2 μl of RNase Inhibitor

(107) Magnetic Bead Processing Enables Sensitive Ligation-Based Detection of HIV Drug Resistance MutationsAnalytical ChemistryFebruary 8, 2022Dalton J. Nelson, Mindy Leelawong, Megan E. Pask, C. William Wester, Muktar H. Aliyu, Frederick R. Haseltonoligonucleotide ligation assay that followed with the use of ProtoScript II reverse transcriptase (NEB #M0368). Each reaction consisted of 0.5 μL of ProtoScript II reverse transcriptase, 4 μL of 5× ProtoScript

(108) GCN5 maintains muscle integrity by acetylating YY1 to promote dystrophin expressionThe Journal of cell biologyFebruary 7, 2022Addicks GC, Zhang H, Ryu D, Vasam G, Green AE, Marshall PL, Patel S, Kang BE, Kim D, Katsyuba E, et al.Scientific), and, for each sample, 1 µg RNA was converted to cDNA using ProtoScript II reverse transcription (M0368; New England Biolabs) in a 20-μl reaction. 0.5 μl of reverse transcription was used per 10-μl qPCR (Luna Universal qPCR Master Mix

(109) Identification of the Cysteine Protease Legumain as a Potential Chronic Hypoxia-Specific Multiple Myeloma Target GeneCellsJanuary 15, 2022Clees AS, Stolp V, Häupl B, Fuhrmann DC, Wempe F, Seibert M, Weber S, Banning A, Tikkanen R, Williams R, et al.Moloney murine leukemia virus reverse transcriptase (ProtoScript II reverse transcriptase, New England Biolabs, #M0368) in a total volume of 20 μL. Real-time PCRs (Opticon 2 reader, MJ research, now BioRad, Dreieich, Germany)

(110) A mutation in transmembrane protein 135 impairs lipid metabolism in mouse eyecupsScientific reportsJanuary 14, 2022Landowski M, Bhute VJ, Takimoto T, Grindel S, Shahi PK, Pattnaik BR, Ikeda S, Ikeda A.using an Oligo d(T)18 primer (#S1316S, NEB, Ipswich, MA) and the ProtoScript II Reverse Transcriptase (#M0368L, NEB, Ipswich, MA). Triplicate reactions for each gene was run in the Roche Lightcycler 480 system using

(111) Multilayer omics analysis reveals a non-classical retinoic acid signaling axis that regulates hematopoietic stem cell identityCell stem cellJanuary 6, 2022Schönberger K, Obier N, Romero-Mulero MC, Cauchy P, Mess J, Pavlovich PV, Zhang YW, Mitterer M, Rettkowski J, Lalioti ME, et al.,Beckman Coulter,A63881 "Deoxynucleotide Solution, Mix",NEB,N0447S ProtoScript II Reverse Transcriptase,NEB,M0368L Murine Rnase Inhibitor,NEB,M0314L "Rnase H, recombinant",NEB,M0297L DNA Polymerase I,NEB,M0209L DAPI,Sigma-Aldrich,D9542 All-trans-retinoic

(112) Orphan cytochrome P450 20a1 CRISPR/Cas9 mutants and neurobehavioral phenotypes in zebrafishScientific ReportsDecember 13, 2021Nadja R. Brun, Matthew C. Salanga, Francisco X. Mora-Zamorano, David C. Lamb, Jared V. Goldstone, John J. Stegemancloning Up to 1 μg of DNA-free RNA was reverse transcribed using ProtoScript II Reverse Transcriptase (NEB, M0368) and anchored oligo dT primers according to the product instructions. T7E1 mutant survey (F0) T7 endonuclease

(113) Characterization of the Leucocyte Immunoglobulin-like Receptor B4 (Lilrb4) Expression in MicrogliaBiologyDecember 9, 2021Kretzschmar F, Piecha R, Jahn J, Potru PS, Spittau B.BioPhotometer D30). Reverse transcription was performed using the ProtoScript II First Strand cDNA Synthesis Kit (M0368L, New England BioLabs, Frankfurt/Main, Germany) according to the manufacturer´s instructions. Quantitative

(114) eIF2B-capturing viral protein NSs suppresses the integrated stress responseNature communicationsDecember 7, 2021Kashiwagi K, Shichino Y, Osaki T, Sakamoto A, Nishimoto M, Takahashi M, Mito M, Weber F, Ikeuchi Y, Iwasaki S, et al.Total RNA kit (Illumina, 20020598), and reverse-transcribed by Protoscript II (New England Biolabs, M0368) for 30 min at 50 °C with a primer (Eurofins, 5′-Phos-NNAGATCGGAAGAGCGTCGTGTAGGGAAAGAG-iSp18-GTGACTGGA

(115) A genome-scale CRISPR interference guide library enables comprehensive phenotypic profiling in yeastBMC GenomicsDecember 1, 2021Nicholas J. McGlincy, Zuriah A. Meacham, Kendra K. Reynaud, Ryan Muller, Rachel Baum, Nicholas T. IngoliaReverse transcription was carried out using 10 ng of purified RNA in a reaction with ProtoScript II (NEB M0368S) using 2.0 pmol NI-1032 as a gene-specific primer (Additional file 8: Table S8). Primer and template

(116) Mammalian SWI/SNF chromatin remodeler is essential for reductional meiosis in malesNature CommunicationsDecember 1, 2021Debashish U. Menon, Oleksandr Kirsanov, Christopher B. Geyer, Terry MagnusoncDNA was synthesized using random primer mix (NEB) and ProtoScript® II reverse transcriptase (NEB; M0368L). Real-time qPCR was performed using Sso Fast EvaGreen supermix (Bio-Rad; 172-5280) on a CFX96 thermocycler

(117) A chemical-enhanced system for CRISPR-Based nucleic acid detectionBiosensors and BioelectronicsNovember 15, 2021Zihan Li, Wenchang Zhao, Shixin Ma, Zexu Li, Yingjia Yao, Teng Fei(primer-free rehydration buffer), 224 mM of MgOAc, 40 U of ProtoScript II Reverse Transcriptase (NEB, #M0368S), 0.5 μM of forward primer, 0.5 μM of reverse primer and UltraPure water to a total volume of 16 μL.

(118) A novel mechanism of enhanced transcription activity and fidelity for influenza A viral RNA-dependent RNA polymeraseNucleic Acids ResearchSeptember 7, 2021Xinzhou Xu, Lu Zhang, Julie Tung Sem Chu, Yuqing Wang, Alex Wing Hong Chin, Tin Hang Chong, Zixi Dai, Leo Lit Man Poon, Peter Pak-Hang Cheung, Xuhui Huangviral RNA was then reverse-transcribed from 5 μl sample by ProtoScript II Reverse Transcriptase (NEB M0368) using the MBTuni-12 primer and amplified for 22 cycles using Q5 Hot Start High-Fidelity DNA Polymerase

(119) Liposome-mediated detection of SARS-CoV-2 RNA-positive extracellular vesicles in plasmaNature nanotechnologySeptember 1, 2021Ning B, Huang Z, Youngquist BM, Scott JW, Niu A, Bojanowski CM, Zwezdaryk KJ, Saba NS, Fan J, Yin XM, et al.3 pmol N gene primer pairs (Supplementary Table 1), 1 μL ProtoScript® II Reverse Transcriptase (NEB M0368L), 3 μL 80 mM MgOAc and 2.6 μL nuclease-free water. The mixture was aliquoted into 3 part for triplicates

(120) Thrombopoietin from hepatocytes promotes hematopoietic stem cell regeneration after myeloablationeLifeAugust 31, 2021Longfei Gao, Matthew Decker, Haidee Chen, Lei Dingalgorithm",Prism,GraphPad,, Other,SuperScript III,ThermoFisher,Cat# 18080093, Other,ProtoScript II,NEB,Cat# M0368S, Other,PELCO Cryo-Embedding compound,"Ted Pella, Inc","Cat# 27,300",

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ProtoScript II Reverse Transcriptase

ProtoScript II Reverse Transcriptase from New England Biolabs

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ProtoScript II Reverse Transcriptase from New England Biolabs

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