ProtoScript II Reverse Transcriptase M0368L from New England Biolabs

Product Specs
ItemProtoScript II Reverse Transcriptase
CompanyNew England Biolabs
Price $198.00 Inquire
Catalog NumberM0368L
Size10000 units
ApplicationsDNA Analysis
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(21) Regulation of a nickel tolerance operon conserved in Mesorhizobium strains from serpentine soilsApplied and Environmental MicrobiologyDecember 23, 2025Kyson T. Jensen, Joel S. GriffittsProtoScript II Buffer, 0.5 mM dNTPs, 10 µM DTT, and 200 U of ProtoScript II Reverse Transcriptase (NEB M0368). The mixture was incubated at 42°C for 1 h. After incubation, the product was purified using a Zymo

(22) Massively Scalable Single-cell Multiomic Profiling of T Cell Repertoire with REFLEXResearch SquareDecember 23, 2025Matthew R. Skene, Zachary J. Thomson, Saransh N. Thomson, Saransh Ilcisin, Matthieu Landreau, Tyanna J. Landreau, Peter J. Stuckey, Micheal Wittig, Chase D. Keller, Troy R. Torgerson, et al.dithiothreitol, 3.3 μL Superase-IN (see above), and 10 μL ProtoScript II RT enzyme (New England Biolabs, M0368L). 60 μL of RT master mix per sample/well was added to pre-existing 40 μL of volume for a final volume

(23) Collision-induced ribosome degradation driven by ribosome competition and translational perturbationsNat CommunDecember 12, 2025Sihan Li, Okuto Shounai, Misaki Kato, Ken Ikeuchi, Toshifumi Inadafragments were then subjected to reverse transcription using ProtoScript II Reverse Transcriptase (# M0368, NEB), circularization with CircLigase™ II ssDNA Ligase (# CL9025K, Epicenter), and subsequent PCR amplification

(24) REFLEX, a Novel Immune Profiling Assay, Combining TCR Repertoire and Multiome at Massively Scalable Single-cell Resolution to Catapult Exploration of T-cell Derived ImmunitybioRxivDecember 2, 2025Matthew R. Hart, Zachary J. Thomson, Saransh N. Kaul, Saskia Ilcisin, Matthieu Landreau, Tyanna J. Stuckey, Peter J. Wittig, Micheal Keller, Chase D. McCann, Troy R. Torgerson, et al.dithiothreitol, 3.3 μL Superase-IN (see above), and 10 μL ProtoScript II RT enzyme (New England Biolabs, M0368L). 60 μL of RT master mix per sample/well was added to pre-existing 40 μL of volume for a final volume

(25) Decapping activators Edc3 and Scd6 act redundantly with Dhh1 in post-transcriptional repression of starvation-induced pathwayseLifeNovember 25, 2025Kumar R, Zhang F, Niphadkar S, Onu C, Vijjamarri AK, Greenberg ML, Laxman S, Hinnebusch AG.column,Zymo Research,Cat # D4060, Commercial assay or kit,Protoscript II,New England Biolabs,Cat # M0368L, Commercial assay or kit,CircLigase ssDNA Ligase,Epicenter,Cat # CL4111K, Commercial assay or kit,Phusion

(26) TGFβ-Smad3 signaling restores cell-autonomous Srsf1-mediated splicing of fibronectin in aged skeletal muscle stem cellsNature CommunicationsNovember 21, 2025Yuguo Liu, Svenja C. Schüler, Simon Dumontier, Frederic Balg, Sonia Bedard, Thibaut Desgeorges, Jerome N. Feige, Pierre-Luc Boudreault, C. Florian Bentzingerthe manufacturer’s instructions. cDNA was generated using ProtoScript II Reverse Transcriptase (NEB, M0368) with 15-mers oligo-dT (Integrated DNA Technologies). For densitometric quantification of cDNA, PCR amplification

(27) The CCR4-NOT deadenylase complex mediates tubulin autoregulation via specific adapters CNOT10 and CNOT11MicroPubl BiolNovember 18, 2025Stephanie L. Sarbanes, J. Robert Hogg, Antonina Roll-Mecakconverted to cDNA using an oligod(T)18 primer (NEB S1316) and Protoscript II reverse transcriptase (NEB M0368) and quantified by RT-qPCR as above, with 5’-biased gene-specific primers including those for GAPDH

(28) Loss of histone macroH2A1.1 causes kidney abnormalities secondary to a change in nutrient metabolizationSci AdvOctober 24, 2025René Winkler, Gemma Comas-Armangué, David Corujo, Adrián Sanz-Moreno, Julia Calzada-Wack, Shubhra Ashish Bhattacharya, Birgit Rathkolb, Nathalia Romanelli Vicente Dragano, Colina X. Qiao, Valentina Chiodi, et al.manufacturer’s instructions. cDNA was obtained using the ProtoScript II Reverse Transcriptase (catalog # M0368, New England Biolabs Inc., Ipswich, MA, USA). A 1:5 dilution of the cDNA was used for PCR with the Macroh2a2_exon_2

(29) The circadian regulator PER1 inhibits osteoclastogenesis by activating inflammatory genesbioRxivSeptember 24, 2025Nobuko Katoku-Kikyo, Elizabeth K. Vu, Samuel Mitchell, Ismael Y. Karkache, Elizabeth W. Bradley, Nobuaki KikyoDS-11 FX+ (Denovix). cDNA was synthesized with ProtoScript II Reverse Transcriptase (New England Biolabs, M0368L). qPCR was performed with the primers listed in Supplementary Table 2 and qPCRBIO SyGreen Blue Mix Lo-ROX

(30) Mapping the genetic landscape of iron metabolism uncovers the SETD2 methyltransferase as a modulator of iron fluxScience AdvancesSeptember 19, 2025Anthony W. Martinelli, Chun-Pei Wu, Tristan Vornbäumen, Hudson W. Coates, Louise H. Jordon, Niek Wit, Jia J. Sia, Anneliese O. Speak, James A. Nathantranscribed using ProtoScript II Reverse Transcriptase (NEB) according to their standard protocol (NEB, #M0368). Template cDNA (40 ng) was amplified using SYBR Green PCR Master Mix (Thermo Fisher Scientific, 4309155)

(31) BACH2 promotes seeding and establishment of long-lived HIV-1 reservoir in memory CD4+ T cellsCell Reports MedicineSeptember 16, 2025Hongbo Gao, Yuhao Li, Ritudwhaj Tiwari, Marilia Pinzone, Xiwen Qin, Kolin M. Clark, Sara K. Nicholson, Tony Yao, Kelly Rome, Michael Scaglione, et al.protein,IDT,1081059 Random Primers,Invitrogen,48190–011 ProtoScript II Reverse Transcriptase,NEB,M0368 "RNase Inhibitor, Murine",NEB,M0314L Oligo(dT)12–18 Primer,Invitrogen,18418012 TaqMan™ Fast Advanced Master Mix for qPCR

(32) Restoration of Osimertinib sensitivity in lung cancer through BRD4 inhibitor-mediated depalmitoylation of mutant EGFR via APT1NPJ Precis OncolAugust 28, 2025Wolong Zhou, Shaoqiang Wang, Zhenyu Zhang, Linfeng Li, Jiebo Zhu, Hang Lin, Heng Zhangmin) generated 200–300 bp fragments for cDNA synthesis using ProtoScript II Reverse Transcriptase (#M0368, New England Biolabs) and dUTP-based strand marking. Adapter-ligated libraries were size-selected (200–500

(33) APOBEC3B Promotes SARS-CoV-2 Through Activation of PKR/eIF2⍺ and AMPD2 DysregulationVirusesAugust 28, 2025Benjamin Fixman, Lavanya Manjunath, Philip Sell, Shanshan Wang, Tamara Margaryan, Connor Qiu, Hanjing Yang, Rémi Buisson, Xiaojiang S. Chentotal RNA was reverse-transcribed into complementary cDNA using Protoscript II (New England Biolabs, #M0368S (Ipswich, MA, USA)) in a 20 µL reaction containing 1 µL of 100µM gene-specific reverse primer or 2 µL

(34) Targeted delivery of diverse biomolecules with engineered bacterial nanosyringesNature BiotechnologyAugust 12, 2025Joseph Kreitz, Victoria Yang, Mirco J. Friedrich, Julie Pham, Rhiannon K. Macrae, Feng Zhangsamples were then used to generate cDNA using ProtoScript II Reverse Transcriptase (New England Biolabs, M0368) and random primers (New England Biolabs, S1330), according to the manufacturer’s protocol. We then

(35) Interleukin-10 augments human endogenous retroviral E1B variant of cd5 in aged T cellsBlood ResAugust 11, 2025Bharat Singh, Smita Kumari, Amit Kumar Kureel, Arunim Shah, Shobhita Katiyar, Chandra Prakash Chaturvedi, Kulwant Singh, Ambak Kumar RaiThermo Scientific, USA) digestion assay. cDNA from RNA was synthesized using M-MLV reverse transcriptase (M0368L, New England BioLab, USA), OligodT (S0131, Thermo Fisher Scientific, USA), random hexamers (S0142, Thermo

(36) Developmental analysis of the cone photoreceptor-less little skate retinabioRxivJuly 31, 2025Chetan C. Rangachar, Denice D. Moran, Mark M. EmersonCatalog #B1043A, Lot #10122162), 1 μL of Protoscript II reverse transcriptase (20 U/μL) (NEB, Catalog #M0368S, Lot #10125099), 0.2 μL of RNase inhibitor (Invitrogen, Catalog #10777-019, Lot #1840422), and 2.8 μL

(37) Chromatin architecture mapping by multiplex proximity taggingMolecular CellJuly 17, 2025Axel Delamarre, Benton Bailey, Jennifer Yavid, Richard Koche, Neeman Mohibullah, Iestyn WhitehousePEG 20K, 0.5 μL RNase inhibitor (Pierce, AM2694), 1μL of Reverse Transcriptase (ProtoScript II, NEB M0368), 3μL of T7 RNA Polymerase (NEB, M0251)) for 1hr at 20°C. RNA was digested with RNase and DNA was migrated

(38) Evaluation of Apoptosis and Cytotoxicity Induction Using a Recombinant Newcastle Disease Virus Expressing Human IFN-γ in Human Prostate Cancer Cells In VitroBiomedicinesJuly 14, 2025Aldo Rojas-Neyra, Katherine Calderón, Brigith Carbajal-Lévano, Gloria Guerrero-Fonseca, Gisela Isasi-Rivas, Ana Chumbe, Ray W. Izquierdo-Lara, Astrid Poma-Acevedo, Freddy Ygnacio, Dora Rios-Matos, et al.deoxyribonucleic acid (cDNA) was generated from RNA using the ProtoScript® II cDNA Synthesis kit (catalog no. M0368, New England Biolabs, Ipswich, MA, USA), according to the manufacturer’s instructions. The cDNA was

(39) Precise measurement of molecular phenotypes with barcode-based CRISPRi systemsGenome BiolMay 25, 2025Joseph H. Lobel, Nicholas T. IngoliaRNA isolation, 4 µg of each sample was reverse transcribed using dT priming with Protoscript II (NEB, M0368L) and subsequently treated with 0.5 µL each of Rnase H and Rnase A (Thermo Fisher, EN0531 and NEB, M0297S)

(40) The anti-diabetic PPARγ agonist Pioglitazone inhibits cell proliferation and induces metabolic reprogramming in prostate cancerMol CancerMay 5, 2025Emine Atas, Kerstin Berchtold, Michaela Schlederer, Sophie Prodinger, Felix Sternberg, Perla Pucci, Christopher Steel, Jamie D. Matthews, Emily R. James, Cécile Philippe, et al.,Thermo Fisher Scientific,#23236 CyQUANT NF cell proliferation assay kit,Thermo Fisher Scientific,#C35006 cDNA synthesis kit,NEB,#M0368 Luna qRT-PCR master mix,NEB,#M3003 RNA isolation kit,NEB,#TS2010S Punch kit,Merck,#MAMP09608 Experimental models

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ProtoScript II Reverse Transcriptase

ProtoScript II Reverse Transcriptase from New England Biolabs

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ProtoScript II Reverse Transcriptase from New England Biolabs

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