ProtoScript II Reverse Transcriptase M0368L from New England Biolabs

Product Specs
ItemProtoScript II Reverse Transcriptase
CompanyNew England Biolabs
Price $198.00 Inquire
Catalog NumberM0368L
Size10000 units
ApplicationsDNA Analysis
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(21) Interleukin-10 augments human endogenous retroviral E1B variant of cd5 in aged T cellsBlood ResAugust 11, 2025Bharat Singh, Smita Kumari, Amit Kumar Kureel, Arunim Shah, Shobhita Katiyar, Chandra Prakash Chaturvedi, Kulwant Singh, Ambak Kumar RaiThermo Scientific, USA) digestion assay. cDNA from RNA was synthesized using M-MLV reverse transcriptase (M0368L, New England BioLab, USA), OligodT (S0131, Thermo Fisher Scientific, USA), random hexamers (S0142, Thermo

(22) Developmental analysis of the cone photoreceptor-less little skate retinabioRxivJuly 31, 2025Chetan C. Rangachar, Denice D. Moran, Mark M. EmersonCatalog #B1043A, Lot #10122162), 1 μL of Protoscript II reverse transcriptase (20 U/μL) (NEB, Catalog #M0368S, Lot #10125099), 0.2 μL of RNase inhibitor (Invitrogen, Catalog #10777-019, Lot #1840422), and 2.8 μL

(23) Chromatin architecture mapping by multiplex proximity taggingMolecular CellJuly 17, 2025Axel Delamarre, Benton Bailey, Jennifer Yavid, Richard Koche, Neeman Mohibullah, Iestyn WhitehousePEG 20K, 0.5 μL RNase inhibitor (Pierce, AM2694), 1μL of Reverse Transcriptase (ProtoScript II, NEB M0368), 3μL of T7 RNA Polymerase (NEB, M0251)) for 1hr at 20°C. RNA was digested with RNase and DNA was migrated

(24) Evaluation of Apoptosis and Cytotoxicity Induction Using a Recombinant Newcastle Disease Virus Expressing Human IFN-γ in Human Prostate Cancer Cells In VitroBiomedicinesJuly 14, 2025Aldo Rojas-Neyra, Katherine Calderón, Brigith Carbajal-Lévano, Gloria Guerrero-Fonseca, Gisela Isasi-Rivas, Ana Chumbe, Ray W. Izquierdo-Lara, Astrid Poma-Acevedo, Freddy Ygnacio, Dora Rios-Matos, et al.deoxyribonucleic acid (cDNA) was generated from RNA using the ProtoScript® II cDNA Synthesis kit (catalog no. M0368, New England Biolabs, Ipswich, MA, USA), according to the manufacturer’s instructions. The cDNA was

(25) Precise measurement of molecular phenotypes with barcode-based CRISPRi systemsGenome BiolMay 25, 2025Joseph H. Lobel, Nicholas T. IngoliaRNA isolation, 4 µg of each sample was reverse transcribed using dT priming with Protoscript II (NEB, M0368L) and subsequently treated with 0.5 µL each of Rnase H and Rnase A (Thermo Fisher, EN0531 and NEB, M0297S)

(26) The anti-diabetic PPARγ agonist Pioglitazone inhibits cell proliferation and induces metabolic reprogramming in prostate cancerMol CancerMay 5, 2025Emine Atas, Kerstin Berchtold, Michaela Schlederer, Sophie Prodinger, Felix Sternberg, Perla Pucci, Christopher Steel, Jamie D. Matthews, Emily R. James, Cécile Philippe, et al.,Thermo Fisher Scientific,#23236 CyQUANT NF cell proliferation assay kit,Thermo Fisher Scientific,#C35006 cDNA synthesis kit,NEB,#M0368 Luna qRT-PCR master mix,NEB,#M3003 RNA isolation kit,NEB,#TS2010S Punch kit,Merck,#MAMP09608 Experimental models

(27) METTL14 regulates chondrogenesis through the GDF5-RUNX-extracellular matrix gene axis during limb developmentNat CommunApril 30, 2025Nobuko Katoku-Kikyo, Hiroko Kawakami, Max Cantor, Yasuhiko Kawakami, Nobuaki KikyoDS-11 FX+ (Denovix). cDNA was synthesized with ProtoScript II Reverse Transcriptase (New England Biolabs, M0368L). qPCR was performed with the primers listed in Supplementary Table 2 and qPCRBIO SyGreen Blue Mix Lo-ROX

(28) Traffic Jam activates the Flamenco piRNA cluster locus and the Piwi pathway to ensure transposon silencing and Drosophila fertilityCell ReportsApril 22, 2025Austin J. Rivera, Jou-Hsuan Roxie Lee, Shruti Gupta, Linda Yang, Raghuveera Kumar Goel, Joseph Zaia, Nelson C. Laupeptides, and recombinant proteins" DNase I,NEB,Cat# M0303S Protoscript II Reverse Transcriptase,NEB,Cat# M0368 5X Protoscript II Buffer,NEB,Cat# M0368 0.1M DTT,NEB,Cat# M0368 10mM dNTP mix,NEB,Cat# N0447 "RNase Inhibitor, Murine

(29) The regulatory architecture of the primed pluripotent cell stateNat CommunApril 9, 2025Bo I. Li, Mariano J. Alvarez, Hui Zhao, Napon Chirathivat, Andrea Califano, Michael M. Shenbarcode-linked oligo-dT primer was added to each well with 5 µl of 5x ProtoScript RT buffer (New England Biolabs M0368). The plate was incubated at 94 °C for 2 min and immediately cooled on ice for at least 5 min, followed

(30) Viperin expression leads to downregulation of mitochondrial genes through misincorporation of ddhCTP by mitochondrial RNA polymeraseJournal of Biological ChemistryApril 1, 2025Srijoni Majhi, Pronay Roy, Minshik Jo, Jiying Liu, Rebecca Hurto, Lydia Freddolino, E. Neil G. MarshThe purified RNA (0.5–1 μg) was reverse transcribed using the ProtoScript II Reverse Transcriptase (M0368 L, NEB) using Random Primer Mix (S1330S, NEB) according to the manufacturer’s protocol. RT-qPCR was

(31) In Vitro Analysis of AKR1D1 Interactions with Clopidogrel: Effects on Enzyme Activity and Gene ExpressionBalkan J Med GenetMarch 12, 2025K Shutevska, Panovska T Kadifkova, Z Zhivikj, Nestorovska A Kapedanovskafrom HepG2 cells.ProtoScript® II Reverse Transcriptase (New England Biolabs, Ipswich, MA, USA; Cat# M0368): Used to synthesize complementary DNA (cDNA) from isolated RNA.XCEED qPCR SG 2x Mix Lo-ROX (Institute

(32) The levels of the long non-coding RNA MALAT1 affect cell viability and modulate TDP-43 binding to mRNA in the nucleusJournal of Biological ChemistryMarch 1, 2025Adarsh Balaji, Aileen C. Button, Simone D. Hall, Jonathan Zhu, Lauren Ellis, Ellen Lavorando, Ethan L. Ashley, Raul Johnson, Einollah Sarikhani, Zeinab Jahed, et al.(10977015). Complementary DNA was synthesized using 1 μg of RNA and the ProtoScript II reverse transcriptase (M0368S, NEB), using the protocol provided by the manufacturer. qPCR was performed on a QuantStudio 3 using

(33) Orphan G protein-coupled receptor GPRC5B controls macrophage function by facilitating prostaglandin E receptor 2 signalingNat CommunFebruary 7, 2025Jeonghyeon Kwon, Haruya Kawase, Kenny Mattonet, Stefan Guenther, Lisa Hahnefeld, Jamal Shamsara, Jan Heering, Michael Kurz, Sina Kirchhofer, Cornelius Krasel, et al.synthesized by reverse transcription of extracted RNA using and ProtoScriptII (New England Biolabs, M0368) according to the manufacturer’s instruction. Quantitative real-time PCR was performed using SYBR green

(34) The impact of glucocorticoid receptor transactivation on context-dependent cell migration dynamicsSci RepFebruary 4, 2025Szonja Polett Pósa, Éva Saskői, Lili Bársony, Lőrinc Pongor, Fanni Fekete, János Papp, Anikó Bozsik, Attila Patócs, Henriett Butzquantitative PCR (qPCR) A total of 750 ng of RNA was transcribed by the ProtoScript® II Reverse Transcriptase (M0368L, New England Biolabs, Ipswich, MA, USA). Gene expression was measured by reverse transcription–quantitative

(35) Developing, Characterizing, and Modeling CRISPR-Based Point-of-Use Pathogen DiagnosticsACS Synthetic BiologyJanuary 17, 2025Jaeyoung K. Jung, Kathleen S. Dreyer, Kate E. Dray, Joseph J. Muldoon, Jithin George, Sasha Shirman, Maria D. Cabezas, Anne E. d’Aquino, Matthew S. Verosloff, Kosuke Seki, et al.cDNA synthesis was initiated by adding 1 U/μL RT at final concentration (NEB #M0277L for AMV RT, NEB #M0368L for ProtoScript II RT, and NEB #M0253L for M-MuLV RT). After 20 min of incubation at 37 °C, 0.005 U/μL

(36) Quantitative profiling of human translation initiation reveals elements that potently regulate endogenous and therapeutically modified mRNAsMolecular CellJanuary 16, 2025Cole J.T. Lewis, Li H. Xie, Shivani Milind Bhandarkar, Danni Jin, Kyrillos Abdallah, Austin S. Draycott, Yixuan Chen, Carson C. Thoreen, Wendy V. Gilbertwith Turbo DNase (Thermo AM2238), 1 μg of total RNA was reserve transcribed with ProtoScript II (NEB M0368S). The expression levels of Nanoluc and GAPDH were determined by qPCR with the following primer sets:

(37) The M1/M2 Macrophage Polarization and Hepatoprotective Activity of Quercetin in Cyclophosphamide-Induced Experimental Liver ToxicityVet Med SciJanuary 10, 2025Ugur Seker, Emre Uyar, Gul Sahika Gokdemir, Deniz Evrim Kavak, Sevgi Irtegun-KandemircDNA was generated using total RNA and synthesized with ProtoScript II Reverse Transcriptase (Cat no: M0368S, New England Biolabs, MA, USA). All qPCRs were performed in triplicate on the LightCycler FastStart DNA MasterPLUS SYBR Green I

(38) An ADPRS variant disrupts ARH3 stability and subcellular localization in children with neurodegeneration and respiratory failureHuman Genetics and Genomics AdvancesJanuary 9, 2025Maxwell Bannister, Sarah Bray, Anjali Aggarwal, Charles Billington, Hai Dang Nguyenisolation. cDNA was generated using ProtoScript II Reverse Transcriptase (New England Biolabs, no. M0368L) and poly(dT) primers. Twenty microliter RT-qPCRs were performed using the PerfeCTa qPCR SuperMix (QuantaBio,

(39) Phosphorylation of endothelial histone H3.3 serine 31 by PKN1 links flow-induced signaling to proatherogenic gene expressionNature Cardiovascular ResearchJanuary 8, 2025Young-June Jin, Guozheng Liang, Rui Li, ShengPeng Wang, Mohamad Wessam Alnouri, Mette Bentsen, Carsten Kuenne, Stefan Günther, Yang Yan, Yongxin Li, et al.transcription was performed using the ProtoScript II Reverse Transcription Kit (New England Biolabs, M0368S). In brief, to synthesize cDNA, 1 µg of total RNA was combined with 0.5 µg of random hexamer primers

(40) Chemical-guided SHAPE sequencing (cgSHAPE-seq) informs the binding site of RNA-degrading chimeras targeting SARS-CoV-2 5' untranslated regionNat CommunJanuary 8, 2025Zhichao Tang, Shalakha Hegde, Siyuan Hao, Manikandan Selvaraju, Jianming Qiu, Jingxin Wang7.4, 2 μL), DTT (100 mM, 1 μL), dNTP (10 mM, 0.5 μL), ProtoScript II (0.5 μL, New England Biolabs, M0368L) and RNase inhibitor (0.2 μL, ApexBio, K1046) were added. The reaction was incubated at 42 °C for 1

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ProtoScript II Reverse Transcriptase

ProtoScript II Reverse Transcriptase from New England Biolabs

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ProtoScript II Reverse Transcriptase from New England Biolabs

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