ProtoScript II Reverse Transcriptase M0368L from New England Biolabs

Product Specs
ItemProtoScript II Reverse Transcriptase
CompanyNew England Biolabs
Price $198.00 Inquire
Catalog NumberM0368L
Size10000 units
ApplicationsDNA Analysis
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(41) METTL14 regulates chondrogenesis through the GDF5-RUNX-extracellular matrix gene axis during limb developmentNat CommunApril 30, 2025Nobuko Katoku-Kikyo, Hiroko Kawakami, Max Cantor, Yasuhiko Kawakami, Nobuaki KikyoDS-11 FX+ (Denovix). cDNA was synthesized with ProtoScript II Reverse Transcriptase (New England Biolabs, M0368L). qPCR was performed with the primers listed in Supplementary Table 2 and qPCRBIO SyGreen Blue Mix Lo-ROX

(42) Traffic Jam activates the Flamenco piRNA cluster locus and the Piwi pathway to ensure transposon silencing and Drosophila fertilityCell ReportsApril 22, 2025Austin J. Rivera, Jou-Hsuan Roxie Lee, Shruti Gupta, Linda Yang, Raghuveera Kumar Goel, Joseph Zaia, Nelson C. Laupeptides, and recombinant proteins" DNase I,NEB,Cat# M0303S Protoscript II Reverse Transcriptase,NEB,Cat# M0368 5X Protoscript II Buffer,NEB,Cat# M0368 0.1M DTT,NEB,Cat# M0368 10mM dNTP mix,NEB,Cat# N0447 "RNase Inhibitor, Murine

(43) The regulatory architecture of the primed pluripotent cell stateNat CommunApril 9, 2025Bo I. Li, Mariano J. Alvarez, Hui Zhao, Napon Chirathivat, Andrea Califano, Michael M. Shenbarcode-linked oligo-dT primer was added to each well with 5 µl of 5x ProtoScript RT buffer (New England Biolabs M0368). The plate was incubated at 94 °C for 2 min and immediately cooled on ice for at least 5 min, followed

(44) Viperin expression leads to downregulation of mitochondrial genes through misincorporation of ddhCTP by mitochondrial RNA polymeraseJournal of Biological ChemistryApril 1, 2025Srijoni Majhi, Pronay Roy, Minshik Jo, Jiying Liu, Rebecca Hurto, Lydia Freddolino, E. Neil G. MarshThe purified RNA (0.5–1 μg) was reverse transcribed using the ProtoScript II Reverse Transcriptase (M0368 L, NEB) using Random Primer Mix (S1330S, NEB) according to the manufacturer’s protocol. RT-qPCR was

(45) In Vitro Analysis of AKR1D1 Interactions with Clopidogrel: Effects on Enzyme Activity and Gene ExpressionBalkan J Med GenetMarch 12, 2025K Shutevska, Panovska T Kadifkova, Z Zhivikj, Nestorovska A Kapedanovskafrom HepG2 cells.ProtoScript® II Reverse Transcriptase (New England Biolabs, Ipswich, MA, USA; Cat# M0368): Used to synthesize complementary DNA (cDNA) from isolated RNA.XCEED qPCR SG 2x Mix Lo-ROX (Institute

(46) The levels of the long non-coding RNA MALAT1 affect cell viability and modulate TDP-43 binding to mRNA in the nucleusJournal of Biological ChemistryMarch 1, 2025Adarsh Balaji, Aileen C. Button, Simone D. Hall, Jonathan Zhu, Lauren Ellis, Ellen Lavorando, Ethan L. Ashley, Raul Johnson, Einollah Sarikhani, Zeinab Jahed, et al.(10977015). Complementary DNA was synthesized using 1 μg of RNA and the ProtoScript II reverse transcriptase (M0368S, NEB), using the protocol provided by the manufacturer. qPCR was performed on a QuantStudio 3 using

(47) Orphan G protein-coupled receptor GPRC5B controls macrophage function by facilitating prostaglandin E receptor 2 signalingNat CommunFebruary 7, 2025Jeonghyeon Kwon, Haruya Kawase, Kenny Mattonet, Stefan Guenther, Lisa Hahnefeld, Jamal Shamsara, Jan Heering, Michael Kurz, Sina Kirchhofer, Cornelius Krasel, et al.synthesized by reverse transcription of extracted RNA using and ProtoScriptII (New England Biolabs, M0368) according to the manufacturer’s instruction. Quantitative real-time PCR was performed using SYBR green

(48) The impact of glucocorticoid receptor transactivation on context-dependent cell migration dynamicsSci RepFebruary 4, 2025Szonja Polett Pósa, Éva Saskői, Lili Bársony, Lőrinc Pongor, Fanni Fekete, János Papp, Anikó Bozsik, Attila Patócs, Henriett Butzquantitative PCR (qPCR) A total of 750 ng of RNA was transcribed by the ProtoScript® II Reverse Transcriptase (M0368L, New England Biolabs, Ipswich, MA, USA). Gene expression was measured by reverse transcription–quantitative

(49) Developing, Characterizing, and Modeling CRISPR-Based Point-of-Use Pathogen DiagnosticsACS Synthetic BiologyJanuary 17, 2025Jaeyoung K. Jung, Kathleen S. Dreyer, Kate E. Dray, Joseph J. Muldoon, Jithin George, Sasha Shirman, Maria D. Cabezas, Anne E. d’Aquino, Matthew S. Verosloff, Kosuke Seki, et al.cDNA synthesis was initiated by adding 1 U/μL RT at final concentration (NEB #M0277L for AMV RT, NEB #M0368L for ProtoScript II RT, and NEB #M0253L for M-MuLV RT). After 20 min of incubation at 37 °C, 0.005 U/μL

(50) Quantitative profiling of human translation initiation reveals elements that potently regulate endogenous and therapeutically modified mRNAsMolecular CellJanuary 16, 2025Cole J.T. Lewis, Li H. Xie, Shivani Milind Bhandarkar, Danni Jin, Kyrillos Abdallah, Austin S. Draycott, Yixuan Chen, Carson C. Thoreen, Wendy V. Gilbertwith Turbo DNase (Thermo AM2238), 1 μg of total RNA was reserve transcribed with ProtoScript II (NEB M0368S). The expression levels of Nanoluc and GAPDH were determined by qPCR with the following primer sets:

(51) The M1/M2 Macrophage Polarization and Hepatoprotective Activity of Quercetin in Cyclophosphamide-Induced Experimental Liver ToxicityVet Med SciJanuary 10, 2025Ugur Seker, Emre Uyar, Gul Sahika Gokdemir, Deniz Evrim Kavak, Sevgi Irtegun-KandemircDNA was generated using total RNA and synthesized with ProtoScript II Reverse Transcriptase (Cat no: M0368S, New England Biolabs, MA, USA). All qPCRs were performed in triplicate on the LightCycler FastStart DNA MasterPLUS SYBR Green I

(52) An ADPRS variant disrupts ARH3 stability and subcellular localization in children with neurodegeneration and respiratory failureHuman Genetics and Genomics AdvancesJanuary 9, 2025Maxwell Bannister, Sarah Bray, Anjali Aggarwal, Charles Billington, Hai Dang Nguyenisolation. cDNA was generated using ProtoScript II Reverse Transcriptase (New England Biolabs, no. M0368L) and poly(dT) primers. Twenty microliter RT-qPCRs were performed using the PerfeCTa qPCR SuperMix (QuantaBio,

(53) Chemical-guided SHAPE sequencing (cgSHAPE-seq) informs the binding site of RNA-degrading chimeras targeting SARS-CoV-2 5' untranslated regionNat CommunJanuary 8, 2025Zhichao Tang, Shalakha Hegde, Siyuan Hao, Manikandan Selvaraju, Jianming Qiu, Jingxin Wang7.4, 2 μL), DTT (100 mM, 1 μL), dNTP (10 mM, 0.5 μL), ProtoScript II (0.5 μL, New England Biolabs, M0368L) and RNase inhibitor (0.2 μL, ApexBio, K1046) were added. The reaction was incubated at 42 °C for 1

(54) Phosphorylation of endothelial histone H3.3 serine 31 by PKN1 links flow-induced signaling to proatherogenic gene expressionNature Cardiovascular ResearchJanuary 8, 2025Young-June Jin, Guozheng Liang, Rui Li, ShengPeng Wang, Mohamad Wessam Alnouri, Mette Bentsen, Carsten Kuenne, Stefan Günther, Yang Yan, Yongxin Li, et al.transcription was performed using the ProtoScript II Reverse Transcription Kit (New England Biolabs, M0368S). In brief, to synthesize cDNA, 1 µg of total RNA was combined with 0.5 µg of random hexamer primers

(55) Mapping the Genetic Architecture of the Adaptive Integrated Stress Response in S. cerevisiaebioRxivDecember 22, 2024Rachel Baum, Jinyoung Kim, Ryan Y. Muller, Nicholas T. Ingoliatranscription was carried out using 4 μg of RNA template with the ProtoScript II reverse transcriptase (NEB #M0368L) and 0.25 μM gene-specific priming (P(UBC6)-mScarlet: NI-1267; P(PCL5)-citrine: NI-1268) according to

(56) The SV40 virus enhancer functions as a somatic hypermutation-targeting element with potential tumorigenic activityTumour Virus ResDecember 1, 2024Šenigl F, Soikkeli AI, Prost S, Schatz DG, Slavková M, Hejnar J, Alinikula J.performed. The prepared RNA was reversely transcribed with Protoscript II Reverse Transcriptase (NEB, M0368) using random primers from the kit. An amount of 2 μL of cDNA was then used for quantitative PCR with

(57) Uncovering the transcriptome-wide RNA modifications in Acinetobacter baumanniiMicrobial GenomicsNovember 20, 2024Kah Ern Ten, Sadequr Rahman, Hock Siew Tansynthesized using oligo d(T)23VN and reverse transcribed using ProtoScript® II Reverse Transcriptase (NEB, M0368S). The RNA template was removed by RNase H (NEB, M0297S), and LongAmp® Taq DNA Polymerase amplified the

(58) KDM5C is a sex-biased brake against germline gene expression programs in somatic lineagesbioRxivNovember 13, 2024Katherine M. Bonefas, Ilakkiya Venkatachalam, Shigeki Iwasereverse transcribed using the ProtoScript II Reverse transcriptase kit from New England Biolabs (NEB #M0368S) and primed with oligo dT. Expression of Kdm5c was detected using the primers 5’-CCCATGGAGGCCAGAGAATAAG-3’

(59) G3BP isoforms differentially affect stress granule assembly and gene expression during cellular stressMolecular Biology of the CellNovember 1, 2024José M. Liboy-Lugo, Carla A. Espinoza, Jessica Sheu-Gruttadauria, Jesslyn E. Park, Albert Xu, Ziad Jowhar, Angela L. Gao, José A. Carmona-Negrón, Torsten Wittmann, Natalia Jura, et al.T4 RNA ligase 2 truncated KQ (NEB, M0373L). Linker-ligated RNA was reverse transcribed with Protoscript II (NEB, M0368L) for 30 min at 50°C. Template RNA was hydrolyzed with 1M NaOH for 20 min at 70°C. Gel slices of reverse

(60) G3BP isoforms differentially affect stress granule assembly and gene expression during cellular stressMol Biol CellOctober 25, 2024José M. Liboy-Lugo, Carla A. Espinoza, Jessica Sheu-Gruttadauria, Jesslyn E. Park, Albert Xu, Ziad Jowhar, Angela L. Gao, José A. Carmona-Negrón, Torsten Wittmann, Natalia Jura, et al.T4 RNA ligase 2 truncated KQ (NEB, M0373L). Linker-ligated RNA was reverse transcribed with Protoscript II (NEB, M0368L) for 30 min at 50°C. Template RNA was hydrolyzed with 1M NaOH for 20 min at 70°C. Gel slices of reverse

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ProtoScript II Reverse Transcriptase

ProtoScript II Reverse Transcriptase from New England Biolabs

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ProtoScript II Reverse Transcriptase from New England Biolabs

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