ProtoScript II Reverse Transcriptase M0368L from New England Biolabs

Product Specs
ItemProtoScript II Reverse Transcriptase
CompanyNew England Biolabs
Price $198.00 Inquire
Catalog NumberM0368L
Size10000 units
ApplicationsDNA Analysis
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(41) The levels of the long non-coding RNA MALAT1 affect cell viability and modulate TDP-43 binding to mRNA in the nucleusJournal of Biological ChemistryMarch 1, 2025Adarsh Balaji, Aileen C. Button, Simone D. Hall, Jonathan Zhu, Lauren Ellis, Ellen Lavorando, Ethan L. Ashley, Raul Johnson, Einollah Sarikhani, Zeinab Jahed, et al.(10977015). Complementary DNA was synthesized using 1 μg of RNA and the ProtoScript II reverse transcriptase (M0368S, NEB), using the protocol provided by the manufacturer. qPCR was performed on a QuantStudio 3 using

(42) Orphan G protein-coupled receptor GPRC5B controls macrophage function by facilitating prostaglandin E receptor 2 signalingNat CommunFebruary 7, 2025Jeonghyeon Kwon, Haruya Kawase, Kenny Mattonet, Stefan Guenther, Lisa Hahnefeld, Jamal Shamsara, Jan Heering, Michael Kurz, Sina Kirchhofer, Cornelius Krasel, et al.synthesized by reverse transcription of extracted RNA using and ProtoScriptII (New England Biolabs, M0368) according to the manufacturer’s instruction. Quantitative real-time PCR was performed using SYBR green

(43) The impact of glucocorticoid receptor transactivation on context-dependent cell migration dynamicsSci RepFebruary 4, 2025Szonja Polett Pósa, Éva Saskői, Lili Bársony, Lőrinc Pongor, Fanni Fekete, János Papp, Anikó Bozsik, Attila Patócs, Henriett Butzquantitative PCR (qPCR) A total of 750 ng of RNA was transcribed by the ProtoScript® II Reverse Transcriptase (M0368L, New England Biolabs, Ipswich, MA, USA). Gene expression was measured by reverse transcription–quantitative

(44) Developing, Characterizing, and Modeling CRISPR-Based Point-of-Use Pathogen DiagnosticsACS Synthetic BiologyJanuary 17, 2025Jaeyoung K. Jung, Kathleen S. Dreyer, Kate E. Dray, Joseph J. Muldoon, Jithin George, Sasha Shirman, Maria D. Cabezas, Anne E. d’Aquino, Matthew S. Verosloff, Kosuke Seki, et al.cDNA synthesis was initiated by adding 1 U/μL RT at final concentration (NEB #M0277L for AMV RT, NEB #M0368L for ProtoScript II RT, and NEB #M0253L for M-MuLV RT). After 20 min of incubation at 37 °C, 0.005 U/μL

(45) Quantitative profiling of human translation initiation reveals elements that potently regulate endogenous and therapeutically modified mRNAsMolecular CellJanuary 16, 2025Cole J.T. Lewis, Li H. Xie, Shivani Milind Bhandarkar, Danni Jin, Kyrillos Abdallah, Austin S. Draycott, Yixuan Chen, Carson C. Thoreen, Wendy V. Gilbertwith Turbo DNase (Thermo AM2238), 1 μg of total RNA was reserve transcribed with ProtoScript II (NEB M0368S). The expression levels of Nanoluc and GAPDH were determined by qPCR with the following primer sets:

(46) The M1/M2 Macrophage Polarization and Hepatoprotective Activity of Quercetin in Cyclophosphamide-Induced Experimental Liver ToxicityVet Med SciJanuary 10, 2025Ugur Seker, Emre Uyar, Gul Sahika Gokdemir, Deniz Evrim Kavak, Sevgi Irtegun-KandemircDNA was generated using total RNA and synthesized with ProtoScript II Reverse Transcriptase (Cat no: M0368S, New England Biolabs, MA, USA). All qPCRs were performed in triplicate on the LightCycler FastStart DNA MasterPLUS SYBR Green I

(47) An ADPRS variant disrupts ARH3 stability and subcellular localization in children with neurodegeneration and respiratory failureHuman Genetics and Genomics AdvancesJanuary 9, 2025Maxwell Bannister, Sarah Bray, Anjali Aggarwal, Charles Billington, Hai Dang Nguyenisolation. cDNA was generated using ProtoScript II Reverse Transcriptase (New England Biolabs, no. M0368L) and poly(dT) primers. Twenty microliter RT-qPCRs were performed using the PerfeCTa qPCR SuperMix (QuantaBio,

(48) Phosphorylation of endothelial histone H3.3 serine 31 by PKN1 links flow-induced signaling to proatherogenic gene expressionNature Cardiovascular ResearchJanuary 8, 2025Young-June Jin, Guozheng Liang, Rui Li, ShengPeng Wang, Mohamad Wessam Alnouri, Mette Bentsen, Carsten Kuenne, Stefan Günther, Yang Yan, Yongxin Li, et al.transcription was performed using the ProtoScript II Reverse Transcription Kit (New England Biolabs, M0368S). In brief, to synthesize cDNA, 1 µg of total RNA was combined with 0.5 µg of random hexamer primers

(49) Chemical-guided SHAPE sequencing (cgSHAPE-seq) informs the binding site of RNA-degrading chimeras targeting SARS-CoV-2 5' untranslated regionNat CommunJanuary 8, 2025Zhichao Tang, Shalakha Hegde, Siyuan Hao, Manikandan Selvaraju, Jianming Qiu, Jingxin Wang7.4, 2 μL), DTT (100 mM, 1 μL), dNTP (10 mM, 0.5 μL), ProtoScript II (0.5 μL, New England Biolabs, M0368L) and RNase inhibitor (0.2 μL, ApexBio, K1046) were added. The reaction was incubated at 42 °C for 1

(50) Mapping the Genetic Architecture of the Adaptive Integrated Stress Response in S. cerevisiaebioRxivDecember 22, 2024Rachel Baum, Jinyoung Kim, Ryan Y. Muller, Nicholas T. Ingoliatranscription was carried out using 4 μg of RNA template with the ProtoScript II reverse transcriptase (NEB #M0368L) and 0.25 μM gene-specific priming (P(UBC6)-mScarlet: NI-1267; P(PCL5)-citrine: NI-1268) according to

(51) The SV40 virus enhancer functions as a somatic hypermutation-targeting element with potential tumorigenic activityTumour Virus ResDecember 1, 2024Šenigl F, Soikkeli AI, Prost S, Schatz DG, Slavková M, Hejnar J, Alinikula J.performed. The prepared RNA was reversely transcribed with Protoscript II Reverse Transcriptase (NEB, M0368) using random primers from the kit. An amount of 2 μL of cDNA was then used for quantitative PCR with

(52) Uncovering the transcriptome-wide RNA modifications in Acinetobacter baumanniiMicrobial GenomicsNovember 20, 2024Kah Ern Ten, Sadequr Rahman, Hock Siew Tansynthesized using oligo d(T)23VN and reverse transcribed using ProtoScript® II Reverse Transcriptase (NEB, M0368S). The RNA template was removed by RNase H (NEB, M0297S), and LongAmp® Taq DNA Polymerase amplified the

(53) KDM5C is a sex-biased brake against germline gene expression programs in somatic lineagesbioRxivNovember 13, 2024Katherine M. Bonefas, Ilakkiya Venkatachalam, Shigeki Iwasereverse transcribed using the ProtoScript II Reverse transcriptase kit from New England Biolabs (NEB #M0368S) and primed with oligo dT. Expression of Kdm5c was detected using the primers 5’-CCCATGGAGGCCAGAGAATAAG-3’

(54) G3BP isoforms differentially affect stress granule assembly and gene expression during cellular stressMolecular Biology of the CellNovember 1, 2024José M. Liboy-Lugo, Carla A. Espinoza, Jessica Sheu-Gruttadauria, Jesslyn E. Park, Albert Xu, Ziad Jowhar, Angela L. Gao, José A. Carmona-Negrón, Torsten Wittmann, Natalia Jura, et al.T4 RNA ligase 2 truncated KQ (NEB, M0373L). Linker-ligated RNA was reverse transcribed with Protoscript II (NEB, M0368L) for 30 min at 50°C. Template RNA was hydrolyzed with 1M NaOH for 20 min at 70°C. Gel slices of reverse

(55) G3BP isoforms differentially affect stress granule assembly and gene expression during cellular stressMol Biol CellOctober 25, 2024José M. Liboy-Lugo, Carla A. Espinoza, Jessica Sheu-Gruttadauria, Jesslyn E. Park, Albert Xu, Ziad Jowhar, Angela L. Gao, José A. Carmona-Negrón, Torsten Wittmann, Natalia Jura, et al.T4 RNA ligase 2 truncated KQ (NEB, M0373L). Linker-ligated RNA was reverse transcribed with Protoscript II (NEB, M0368L) for 30 min at 50°C. Template RNA was hydrolyzed with 1M NaOH for 20 min at 70°C. Gel slices of reverse

(56) CRISPRi with barcoded expression reporters dissects regulatory networks in human cellsbioRxivSeptember 11, 2024Jinyoung Kim, Ryan Y. Muller, Eliana R. Bondra, Nicholas T. Ingoliasynthesize cDNA, 1 μg total RNA was reverse transcribed using dT priming with Protoscript II (NEB, M0368L). qPCR was conducted using DyNAmo qPCR mastermix (ThermoFisher, F410L) according to manufacturer protocols

(57) Precise measurement of molecular phenotypes with barcode-based CRISPRi systemsbioRxivSeptember 10, 2024Joseph H. Lobel, Nicholas T. IngoliaRNA isolation, 4 μg of each sample was reverse transcribed using dT priming with Protoscript II (NEB, M0368L) and subsequently treated with 0.5 μL each of Rnase H and Rnase A (Thermo Fisher, EN0531 and NEB M0297S)

(58) BMP4-Induced Suppression of Breast Cancer Metastasis Is Associated with Inhibition of Cholesterol BiosynthesisInternational Journal of Molecular SciencesAugust 23, 2024Lap Hing Chi, Andrew D. Redfern, Terry C. C. Lim Kam Sian, Ian P. Street, Allan D. Burrows, Suraya Roslan, Roger J. Daly, Robin L. Andersonsynthesised using the ProtoScript II reverse transcriptase (New England Biolabs, Ipswich, MA, USA, #M0368) with random pentadecamers. Gene expression was determined using the SYBR green real-time PCR master

(59) Development of a highly sensitive platform for protein-protein interaction detection and regulation of T cell functionProceedings of the National Academy of Sciences of the United States of AmericaAugust 13, 2024Hayashi H, Mak TW, Tanaka Y, Kubo Y, Izumida M, Urae R, Matsuyama T.conducted for 60 min at 42 °C from 1 μg of total RNA using ProtoScript II Reverse Transcriptase (NEB # M0368L) and subjected to PCR using the specific primers (for anti-CD3ε-scFv-TM) listed in SI Appendix, Table

(60) Molecular consequences of acute versus chronic CDK12 loss in prostate carcinoma nominates distinct therapeutic strategiesbioRxiv : the preprint server for biologyJuly 19, 2024Frank S, Persse T, Coleman I, Bankhead A 3rd, Li D, De-Sarkar N, Wilson D, Rudoy D, Vashisth M, Galipeau P, et al.and quantified by Qubit (Thermo Q10211). Reverse transcription was performed with ProtoScript II (NEB M0368) using 4μM poly-dT plus 1μM random hexamer primers. qPCR was performed in 384-well plates (15μL reactions)

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ProtoScript II Reverse Transcriptase

ProtoScript II Reverse Transcriptase from New England Biolabs

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ProtoScript II Reverse Transcriptase from New England Biolabs

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