ProtoScript II Reverse Transcriptase M0368S from New England Biolabs

Product Specs
ItemProtoScript II Reverse Transcriptase
CompanyNew England Biolabs
Price $99.00 Inquire
Catalog NumberM0368S
Size4000 units
ApplicationsDNA Analysis
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(161) Adaptive substitutions underlying cardiac glycoside insensitivity in insects exhibit epistasis in vivoeLifeAugust 27, 2019Andrew M Taverner et al.transcribed into cDNA with the use of random primers and ProtoScript II Reverse Transcriptase (NEB M0368S). Two sets of primers for ATPα1 were designed and used in subsequent PCR, multiplexing and sequencing.

(162) Identification of murine gammaherpesvirus 68 miRNA-mRNA hybrids reveals miRNA target conservation among gammaherpesviruses including host translation and protein modification machineryPLoS pathogensAugust 1, 2019Bullard WL, Kara M, Gay LA, Sethuraman S, Wang Y, Nirmalan S, Esemenli A, Feswick A, Hoffman BA, Renne R, et al.cDNA was synthesized from 1μg of total RNA using the NEB ProtoScript II Reverse Transcriptase (NEB, M0368S) per the manufacturer’s instructions. Quantification of selected genes were performed on an iCycler

(163) A Gammaherpesvirus MicroRNA Targets EWSR1 (Ewing Sarcoma Breakpoint Region 1) In Vivo To Promote Latent Infection of Germinal Center B CellsmBioJuly 30, 2019Wang Y, Feldman ER, Bullard WL, Tibbetts SA.was reverse transcribed into cDNA using ProtoScript II reverse transcriptase (New England Biolabs, M0368S) with random primer mix (New England Biolabs, S1330S). All qPCRs were performed in triplicate on the

(164) Telomere Dysfunction Induces Sirtuin Repression that Drives Telomere-Dependent DiseaseCell metabolismJune 4, 2019Amano H, Chaudhury A, Rodriguez-Aguayo C, Lu L, Akhanov V, Catic A, Popov YV, Verdin E, Johnson H, Stossi F, et al.,Thermo Fisher Scientific,Cat# 15596026 DNase I,New England Biolabs,Cat# M0303 Protoscript II,New England Biolabs,Cat# M0368 SensiFAST™ Probes,Bioline,Cat# BIO-86005 SensiFAST™ SYBR Kit,Bioline,Cat# Bio-98020 Cycloheximide,Sigma,Cat#

(165) Cellular response to small molecules that selectively stall protein synthesis by the ribosomePLOS GeneticsMarch 15, 2019Nadège Liaud, Max A. Horlbeck, Luke A. Gilbert, Ketrin Gjoni, Jonathan S. Weissman, Jamie H. D. CateNew England Biolabs, M0242S). First-strand cDNA was synthesized using Protoscript II (200 U, New England Biolabs, M0368L) and circularized using Circligase I (100 U, Epicentre, CL4111K). Ribosomal RNA was depleted using custom

(166) High-resolution mapping of a standardized transcriptional reporter reveals positions of high and low transcription in the Escherichia coli chromosomeCell SystemsMarch 1, 2019Scott A. Scholz, Rucheng Diao, Michael B. Wolfe, Elayne M. Fivenson, Xiaoxia Nina Lin, Peter L. Freddolinopolymerase,NEB,Cat#M0493 10X ACGU,Teknova,cat # M2103 5x Supplement EZ,Teknova,cat # M2008 Protoscript II,NEB,Cat#M0368 AscI,NEB,Cat#R0558 PvuII,NEB,Cat#R0151 EvaGreen,Biotium,Catalog #: 31000 RNAProtect Bacteria Reagent,Qiagen,Cat

(167) Heterogeneous Responses of Hematopoietic Stem Cells to Inflammatory Stimuli Are Altered with AgeCell reportsDecember 11, 2018Mann M, Mehta A, de Boer CG, Kowalczyk MS, Lee K, Haldeman P, Rogel N, Knecht AR, Farouq D, Regev A, et al.(100x),Corning,30–002-Cl 0.25% Trypsin,Corning,25–053-Cl TRIzol Reagent,Invitrogen,15596026 Protoscript II,New England Biolabs,M0368L Antarctic phosphatase,New England Biolabs,M0289S Puromycin,InvivoGen,Cat#ant-pr-1 Murine SCF,PeproTech,250–03 Murine

(168) A low-cost paper-based synthetic biology platform for analyzing gut microbiota and host biomarkersNature CommunicationsDecember 1, 2018Melissa K. Takahashi, Xiao Tan, Aaron J. Dy, Dana Braff, Reid T. Akana, Yoshikazu Furuta, Nina Donghia, Ashwin Ananthakrishnan, James J. Collinsmix: 5 U/ml RNaseH (New England Biolabs M0297L), 1000 U/ml reverse transcriptase (New England Biolabs, M0368L), 2500 U/ml T7 RNA polymerase (New England Biolabs, M0251L), 43.75 mg/ml BSA. Initial denaturation of

(169) Promotion of Myoblast Differentiation by Fkbp5 via Cdk4 IsomerizationCell reportsNovember 27, 2018Ruiz-Estevez M, Staats J, Paatela E, Munson D, Katoku-Kikyo N, Yuan C, Asakura Y, Hostager R, Kobayashi H, Asakura A, et al.Fisher Scientific). cDNA was synthesized with ProtoScript II Reverse Transcriptase (New England Biolabs, M0368L). Quantitative PCR (qPCR) was performed with the primers listed in Table S2 and a GoTaq qPCR Master Mix

(170) Translational Reprogramming Provides a Blueprint for Cellular AdaptationCell Chemical BiologyNovember 1, 2018Max Berman Ferretti, Jennifer Louise Barre, Katrin KarbsteinS445 Tris-HCL,Fisher Scientific,CAT# BP1757 RQ1 DNase,Promega,CAT# M6101 ProtoScriptII,New England Biolabs,CAT# M0368S Oligo dT,New England Biolabs,CAT# S1316S Excella™ SYBR MasterMix IQ,Worldwide Medical Products,CAT#

(171) Piezo1 and G_q/G₁₁ promote endothelial inflammation depending on flow pattern and integrin activationThe Journal of experimental medicineOctober 1, 2018Albarrán-Juárez J, Iring A, Wang S, Joseph S, Grimm M, Strilic B, Wettschureck N, Althoff TF, Offermanns S.DNA synthesis was performed using the ProtoScript II Reverse Transcription kit (New England BioLabs, M0368S). Quantitative real-time PCR was performed using primers designed with the online tool provided by Roche

(172) A comprehensive spatial-temporal transcriptomic analysis of differentiating nascent mouse lens epithelial and fiber cellsExperimental Eye ResearchOctober 1, 2018Yilin Zhao, Deyou Zheng, Ales Cvekldescribed above (2.1) was reverse transcribed into cDNA with ProtoScript II reverse Transcriptase (NEB: M0368) with Random Hexamer, following manufacturer’s instructions. The cDNA was diluted 5* and qPCR was conducted

(173) Divergent Hemogen genes of teleosts and mammals share conserved roles in erythropoiesis: analysis using transgenic and mutant zebrafishBiology openAugust 28, 2018Peters MJ, Parker SK, Grim J, Allard CAH, Levin J, Detrich HW 3rd.DNase treated RNA was reverse transcribed with a polyT(23) primer using Protoscript II RT-PCR kit (NEB; M0368S). Target genes were amplified in triplicate from cDNA by qRT-PCR with 1 µM primers (Table S1). Standard

(174) Numerous recursive sites contribute to accuracy of splicing in long introns in fliesPLOS GeneticsAugust 27, 2018Athma A. Pai, Joseph M. Paggi, Paul Yan, Karen Adelman, Christopher B. Burgedescribed above) and reverse transcribed to first-strand cDNA using ProtoScript II Reverse Transcriptase (M0368S, NEB) primed with random hexamers according to manufacturer’s protocol. cDNA was diluted 1:5 and 1uL

(175) Heterogeneous Responses of Hematopoietic Stem Cells to Inflammatory Stimuli are Altered with AgeExperimental HematologyAugust 1, 2018Mati Mann, Arnav Mehta, Carl de Boer, Monika Kowalczyk, Aviv Regev, David BaltimoreTrypsin Corning 25-053-Cl TRIzol Reagent Invitrogen 15596026 Protoscript II New England Biolabs M0368L Antarctic phosphatase New England Biolabs M0289S Puromycin InvivoGen Cat#ant-pr-1 Murine SCF PeproTech 250-03 Murine

(176) Engineered protein degradation of farnesyl pyrophosphate synthase is an effective regulatory mechanism to increase monoterpene production in Saccharomyces cerevisiaeMetabolic EngineeringMay 1, 2018Bingyin Peng, Lars K. Nielsen, Sotirios C. Kampranis, Claudia E. Vickersfor first-strand cDNA synthesis in a 10 μl reaction using ProtoScript® II Reverse Transcriptase (NEB #M0368). The diluted cDNA was used as the template for quantitative real-time (qRT) PCR (primers are listed

(177) La-related protein 1 (LARP1) repression of TOP mRNA translation is mediated through its cap-binding domain and controlled by an adjacent regulatory regionNucleic Acids ResearchFebruary 16, 2018Lucas Philippe, Jean-Jacques Vasseur, Françoise Debart, Carson C Thoreenmanufacturers instructions. cDNA was synthesized with the ProtoScript II reverse transcriptase (NEB M0368) using oligo dT18 primers and 100 ng total RNA (quantified by Nanodrop, A260/A280 > 1.8) according to

(178) PD-1 is a haploinsufficient suppressor of T cell lymphomagenesisNatureDecember 7, 2017Wartewig T, Kurgyis Z, Keppler S, Pechloff K, Hameister E, Öllinger R, Maresch R, Buch T, Steiger K, Winter C, et al.from eGFP+ FACS-sorted spleen-derived lymphocytes using spin-columns and then reverse transcribed (M0368L, NEB, Ipswich, Massachusetts, USA). The Pdcd1 and eGFP cDNA was simultaneously quantified using transcript-specific

(179) Consideration of non-canonical splice sites improves gene prediction on the Arabidopsis thaliana Niederzenz-1 genome sequenceBMC Research NotesDecember 4, 2017Boas Pucker, Daniela Holtgräwe, Bernd Weisshaarprotocol. cDNA synthesis was carried out using 1 µg of total RNA and ProtoScript II Reverse Transcriptase (M0368L, New England Biolabs) based on the suppliers’ protocol. Q5 High-Fidelity DNA polymerase (M0491L, New England Biolabs

(180) Coupling gene regulatory patterns to bioprocess conditions to optimize synthetic metabolic modules for improved sesquiterpene production in yeastBiotechnology for BiofuelsDecember 1, 2017Bingyin Peng, Manuel R. Plan, Alexander Carpenter, Lars K. Nielsen, Claudia E. Vickersfor firststrand cDNA synthesis in a 10 μL reaction using ProtoScript® II Reverse Transcriptase (NEB #M0368). The diluted cDNA was used as the template for quantitative real-time (qRT) PCR (primers are listed

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ProtoScript II Reverse Transcriptase

ProtoScript II Reverse Transcriptase from New England Biolabs

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ProtoScript II Reverse Transcriptase from New England Biolabs

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